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The map label for this gene is msbB [H]

Identifier: 160875025

GI number: 160875025

Start: 2279399

End: 2280337

Strand: Direct

Name: msbB [H]

Synonym: Sbal195_1910

Alternate gene names: 160875025

Gene position: 2279399-2280337 (Clockwise)

Preceding gene: 160875024

Following gene: 160875031

Centisome position: 42.63

GC content: 48.78

Gene sequence:

>939_bases
TTGTCAAGACAGCCCAAACATTTCGACCGTCGCTTTAAACTCGCCTTATTACATCCTAAATACTGGGCCACTTGGTTGGC
GATTGGTCTATTAGTGATTTTCGGACTCATGCCCGCATGGCTGCGTGATCCCATTGCAAGATTATTGGCAAAAATAGTGA
TGCCAATTGCCAAAAAGCCCATTAGTGTTGCAAGAGCCAACTTAAGAGCGTGCTTTCCTGAACAATCTGCCGCCGAGATC
GAAGCATTAATCCGTGAGAACGTAAAACACTTCGTTTTAGTCTTGTTAGCTCAAGGCGAATTGCTGGTGCGCTCAAAAGC
GCATTTGCGTCGTCGAGTGAATTTAATCGGCTTTGAGCATGTGACGAAAGCACAAGCAGCGGGGCAACCGATCATTTTCA
TCATGCCCCATGTGTGGCCAATCGATTATGCCGGCTTACGCCTTAACCTAGAGTTGCCTATGGTGACTATGGCCAAAGCC
CATAAAAACGGCTTATTCAATTGGTTTAGTAATCGTGTGCGCAGCAGTTGTGGTGGCCATGTGTATATGCGCGAGGCGGG
CATTCGGGCGTTATTGGCTGAGCTTAAGCGCGGTAACAGTTTCTTTTATCTTCCCGATGAAGATTTAGGCCCAGAACAAA
GCGTGTTTGCACCTTTTTTAGGCACAGTGAAGGCAACGCTGCCTGTCGTTGGGCGTTTAGCACAGGCGGGTAATGCCCAA
GTGCTACCTGTTAAGATTGGTTACAACCAAGCGACACGTCAGTTTGATCTCACTGTGATGACGGCCGTTGCGCCTGAAGA
CATGGTGGGCAAAGAAAACGAGGCGTTGGCACTGAACAAAGTGGTCGAGCAAGTGATCTTAGCTTATCCAGAGCAGTATA
TGTGGTTCCTGCGGGTGCTCAAGACGCGCCCAGAGGGTGAAGCTTCTATCTACAGCTGA

Upstream 100 bases:

>100_bases
GTTTGTTTGATGGGAAAGCCACTCCTTAGAGAGTGGCTTTCCTGTTTATTTTTTTGTGCAATATTTCAACTATTCTTATC
CACTGTCTGAGGTAAATTCT

Downstream 100 bases:

>100_bases
TGTAGCGTGACTGAAATAGTGTGTTTTAAAATGGCTTTGAGCGCGAAGTGCTTGAAGCCATTTTTGTGTTGAAGGGCGTT
CTTTATTGAGCACTCTTTAT

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSRQPKHFDRRFKLALLHPKYWATWLAIGLLVIFGLMPAWLRDPIARLLAKIVMPIAKKPISVARANLRACFPEQSAAEI
EALIRENVKHFVLVLLAQGELLVRSKAHLRRRVNLIGFEHVTKAQAAGQPIIFIMPHVWPIDYAGLRLNLELPMVTMAKA
HKNGLFNWFSNRVRSSCGGHVYMREAGIRALLAELKRGNSFFYLPDEDLGPEQSVFAPFLGTVKATLPVVGRLAQAGNAQ
VLPVKIGYNQATRQFDLTVMTAVAPEDMVGKENEALALNKVVEQVILAYPEQYMWFLRVLKTRPEGEASIYS

Sequences:

>Translated_312_residues
MSRQPKHFDRRFKLALLHPKYWATWLAIGLLVIFGLMPAWLRDPIARLLAKIVMPIAKKPISVARANLRACFPEQSAAEI
EALIRENVKHFVLVLLAQGELLVRSKAHLRRRVNLIGFEHVTKAQAAGQPIIFIMPHVWPIDYAGLRLNLELPMVTMAKA
HKNGLFNWFSNRVRSSCGGHVYMREAGIRALLAELKRGNSFFYLPDEDLGPEQSVFAPFLGTVKATLPVVGRLAQAGNAQ
VLPVKIGYNQATRQFDLTVMTAVAPEDMVGKENEALALNKVVEQVILAYPEQYMWFLRVLKTRPEGEASIYS
>Mature_311_residues
SRQPKHFDRRFKLALLHPKYWATWLAIGLLVIFGLMPAWLRDPIARLLAKIVMPIAKKPISVARANLRACFPEQSAAEIE
ALIRENVKHFVLVLLAQGELLVRSKAHLRRRVNLIGFEHVTKAQAAGQPIIFIMPHVWPIDYAGLRLNLELPMVTMAKAH
KNGLFNWFSNRVRSSCGGHVYMREAGIRALLAELKRGNSFFYLPDEDLGPEQSVFAPFLGTVKATLPVVGRLAQAGNAQV
LPVKIGYNQATRQFDLTVMTAVAPEDMVGKENEALALNKVVEQVILAYPEQYMWFLRVLKTRPEGEASIYS

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=309, Percent_Identity=32.6860841423948, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87082094, Length=307, Percent_Identity=30.6188925081433, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1787292, Length=304, Percent_Identity=28.9473684210526, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35129; Mature: 34998

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQPKHFDRRFKLALLHPKYWATWLAIGLLVIFGLMPAWLRDPIARLLAKIVMPIAKKP
CCCCCCCHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ISVARANLRACFPEQSAAEIEALIRENVKHFVLVLLAQGELLVRSKAHLRRRVNLIGFEH
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VTKAQAAGQPIIFIMPHVWPIDYAGLRLNLELPMVTMAKAHKNGLFNWFSNRVRSSCGGH
HHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCE
VYMREAGIRALLAELKRGNSFFYLPDEDLGPEQSVFAPFLGTVKATLPVVGRLAQAGNAQ
EEEHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VLPVKIGYNQATRQFDLTVMTAVAPEDMVGKENEALALNKVVEQVILAYPEQYMWFLRVL
EEEEEECCCCCCCEEEEEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
KTRPEGEASIYS
HCCCCCCCCCCC
>Mature Secondary Structure 
SRQPKHFDRRFKLALLHPKYWATWLAIGLLVIFGLMPAWLRDPIARLLAKIVMPIAKKP
CCCCCCHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ISVARANLRACFPEQSAAEIEALIRENVKHFVLVLLAQGELLVRSKAHLRRRVNLIGFEH
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VTKAQAAGQPIIFIMPHVWPIDYAGLRLNLELPMVTMAKAHKNGLFNWFSNRVRSSCGGH
HHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCE
VYMREAGIRALLAELKRGNSFFYLPDEDLGPEQSVFAPFLGTVKATLPVVGRLAQAGNAQ
EEEHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VLPVKIGYNQATRQFDLTVMTAVAPEDMVGKENEALALNKVVEQVILAYPEQYMWFLRVL
EEEEEECCCCCCCEEEEEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
KTRPEGEASIYS
HCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]