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The map label for this gene is pheS [H]

Identifier: 160875022

GI number: 160875022

Start: 2275596

End: 2276591

Strand: Direct

Name: pheS [H]

Synonym: Sbal195_1907

Alternate gene names: 160875022

Gene position: 2275596-2276591 (Clockwise)

Preceding gene: 160875020

Following gene: 160875023

Centisome position: 42.56

GC content: 45.38

Gene sequence:

>996_bases
GTGGAAGAGAAAATGCAGCAGTTAACTGAGATCGTAGAACAAGCCTTAGTCATTATTGACCAGGCCAGTGATCTAAAAGC
ACTGGATGATATCCGTGTCGATTACCTGGGTAAGAAAGGTAAGATCACCGACATGATGAAAATGATGGGTAGCTTAAGCC
CTGAAGAAAAGCCAGCCTTTGGCCAAGCGGTCAATGATGCTAAACAGGCGATTCAACAAAAATTAACTGAGCGCATCGAT
GGTCTGAAGAGTTCTGAACTGGAAGCCAAATTAATCGCTGAAAAGATTGATGTGACTTTGCCAGGCCGCACCCAAGAGAT
CGGTGGCTTACATCCAGTGACTCGCACCATTGAACGTATCGAAACCTTTTTCGGGGAATTAGGGTTCTCGGTAAAGCAGG
GTCCTGAAATTGAAGATGATTTCCACAATTTCGATGCGCTGAATATCTCTGAGCACCACCCAGCCCGTGCCGATCATGAT
ACCTTTTATTTCAATCCTAAACTGATGTTGCGTACCCAAACTTCTGGCGTGCAAATCCGTACTATGGAAACTGAAAAGCC
ACCATTACGCATTATTTCGCCGGGTCGTGTTTATCGTAACGATTACGACCAAACGCACACACCAATGTTCCACCAAGTGG
AAGGGCTGTTGGTTGATGAGCATGTGAACTTTGCCGAATTGAAAGGCATTTTGCATGACTTCCTGCGTAACTTCTTCGAA
GAAGATCTGCAAGTGCGTTTCCGTCCATCTTACTTCCCATTCACTGAACCTTCAGCGGAAGTGGACGTGATGGGCAAAAA
TGGTAAATGGCTAGAAGTGCTTGGCTGCGGTATGGTACATCCGAATGTGCTGCGCAGCGTTGGCATAGATCCAGAAAAAT
ACTCTGGTTTTGCTTTTGGTATGGGTGTCGAGCGCTTAACTATGTTGCGTTACGGCGTAAACGATTTACGTGCGTTTTTC
GAAAACGACCTACGTTTCCTGAAGCAATTTAAATAA

Upstream 100 bases:

>100_bases
AGCTAGCAAAGCGTCTGGCATTAACGCCCGATAACCTGAGCACCCAAATAGCCTGAAAGTTTGGCGCCCATAGCACTTGT
TTATCAAGCGCGACTATGCA

Downstream 100 bases:

>100_bases
CGGAGCTGTAATAGCATGAAATTTAGCGAATCTTGGCTTCGTGAATGGGTAAACCCTTCAGTCAGCCGTGAAGCATTATC
CCACCAGATCACTATGGCCG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MEEKMQQLTEIVEQALVIIDQASDLKALDDIRVDYLGKKGKITDMMKMMGSLSPEEKPAFGQAVNDAKQAIQQKLTERID
GLKSSELEAKLIAEKIDVTLPGRTQEIGGLHPVTRTIERIETFFGELGFSVKQGPEIEDDFHNFDALNISEHHPARADHD
TFYFNPKLMLRTQTSGVQIRTMETEKPPLRIISPGRVYRNDYDQTHTPMFHQVEGLLVDEHVNFAELKGILHDFLRNFFE
EDLQVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFF
ENDLRFLKQFK

Sequences:

>Translated_331_residues
MEEKMQQLTEIVEQALVIIDQASDLKALDDIRVDYLGKKGKITDMMKMMGSLSPEEKPAFGQAVNDAKQAIQQKLTERID
GLKSSELEAKLIAEKIDVTLPGRTQEIGGLHPVTRTIERIETFFGELGFSVKQGPEIEDDFHNFDALNISEHHPARADHD
TFYFNPKLMLRTQTSGVQIRTMETEKPPLRIISPGRVYRNDYDQTHTPMFHQVEGLLVDEHVNFAELKGILHDFLRNFFE
EDLQVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFF
ENDLRFLKQFK
>Mature_331_residues
MEEKMQQLTEIVEQALVIIDQASDLKALDDIRVDYLGKKGKITDMMKMMGSLSPEEKPAFGQAVNDAKQAIQQKLTERID
GLKSSELEAKLIAEKIDVTLPGRTQEIGGLHPVTRTIERIETFFGELGFSVKQGPEIEDDFHNFDALNISEHHPARADHD
TFYFNPKLMLRTQTSGVQIRTMETEKPPLRIISPGRVYRNDYDQTHTPMFHQVEGLLVDEHVNFAELKGILHDFLRNFFE
EDLQVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFF
ENDLRFLKQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=257, Percent_Identity=33.4630350194552, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI5729820, Length=278, Percent_Identity=32.7338129496403, Blast_Score=128, Evalue=7e-30,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=73.7003058103976, Blast_Score=520, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI32566635, Length=238, Percent_Identity=35.2941176470588, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17508957, Length=259, Percent_Identity=30.8880308880309, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=259, Percent_Identity=30.8880308880309, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321087, Length=366, Percent_Identity=28.4153005464481, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6325304, Length=152, Percent_Identity=33.5526315789474, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI18858079, Length=258, Percent_Identity=31.7829457364341, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137424, Length=289, Percent_Identity=29.0657439446367, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 37901; Mature: 37901

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKMQQLTEIVEQALVIIDQASDLKALDDIRVDYLGKKGKITDMMKMMGSLSPEEKPAF
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCH
GQAVNDAKQAIQQKLTERIDGLKSSELEAKLIAEKIDVTLPGRTQEIGGLHPVTRTIERI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEECCCCCHHHCCCCHHHHHHHHH
ETFFGELGFSVKQGPEIEDDFHNFDALNISEHHPARADHDTFYFNPKLMLRTQTSGVQIR
HHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCEEEE
TMETEKPPLRIISPGRVYRNDYDQTHTPMFHQVEGLLVDEHVNFAELKGILHDFLRNFFE
EECCCCCCEEEECCCHHHCCCCCCCCCCHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHH
EDLQVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFG
HHHEEEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEEC
MGVERLTMLRYGVNDLRAFFENDLRFLKQFK
CCHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEEKMQQLTEIVEQALVIIDQASDLKALDDIRVDYLGKKGKITDMMKMMGSLSPEEKPAF
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCH
GQAVNDAKQAIQQKLTERIDGLKSSELEAKLIAEKIDVTLPGRTQEIGGLHPVTRTIERI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEECCCCCHHHCCCCHHHHHHHHH
ETFFGELGFSVKQGPEIEDDFHNFDALNISEHHPARADHDTFYFNPKLMLRTQTSGVQIR
HHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCEEEE
TMETEKPPLRIISPGRVYRNDYDQTHTPMFHQVEGLLVDEHVNFAELKGILHDFLRNFFE
EECCCCCCEEEECCCHHHCCCCCCCCCCHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHH
EDLQVRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFG
HHHEEEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEEC
MGVERLTMLRYGVNDLRAFFENDLRFLKQFK
CCHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA