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The map label for this gene is mltB [H]

Identifier: 160875019

GI number: 160875019

Start: 2271797

End: 2272909

Strand: Direct

Name: mltB [H]

Synonym: Sbal195_1904

Alternate gene names: 160875019

Gene position: 2271797-2272909 (Clockwise)

Preceding gene: 160875018

Following gene: 160875020

Centisome position: 42.49

GC content: 48.61

Gene sequence:

>1113_bases
ATGCATCGATACCGTGCTTATCTAACCCAAGCTTATCAAACCAATACTTACCCAGTTAAGGTTTGTCACTTTGGGGTTTA
TCAGAATAAGCTTTATCGACTCGCAGCATATATCTTCGCTCCCATCAGGGCATTTGTCTGTTTTATCATTTGGCTGAGTG
CATGCAGTTTGCCTGCCATGGCGCAGCAGTCGGATGAGAAAATCAGCTTTAGTGATTATTTAATTCAGTTAAAGCAAGAA
GCCCGTCTTCAGGGCATCAGCCAAACGACCTTAGATTTAGCCTTTCCCCAGATCAAACTCTTCAAAAAAGCCCGAGCGAC
AGACAGCCTCCAGACAGAAAAAACTCACAACCTCGATACTTATCTCGCCGACACTGTGCCCGAGTGGAAAGTTGACACCG
CACGGGTGCTGTTTAAAGAGCATGAACAACAACTCAACGCCATCGCCAGCCAATACTCTGTTCAGCCACGCTTCTTAGTC
GCGCTCTGGGGGCTTGTCTCCGACTTTGGTGAAGCTTCTGGCGATTATCCTGTGCTATCTGTGACAGCGTCACTGGCCTT
TAGCGGCGAGCGCGAAGCTTTCTTCAAAAAAGAGTTTATTGCAGCGCTCAGCATAATGGATAAAGATCATATCGGTTTTC
AAGCTCTTAAAAGTCGTTGGAATGGCGCCATGGGACAAACGCAGCTGATGCCAACGGATTATCTTGCCTATGGGCAGGAC
GGCGATGGCGACGGTAAGAAAGACATTTGGCACAACACACAAGATGCGTTTGCCTCCGCGGCGTATTTGCTACAGCAACT
CGGTTGGAACGGCAATGATACTTGGGGGCGACAGGTACAAGTGCCCGCAACGCTTGATTTGGCCGTCGCTGGATTAGATA
AACCCCAGTCCTTAGCCCAGTGGCAAACTCTCGGTATTCGCAAGTTTGATCATACCGATTTGCCGTCCCGCGAAGACATC
AACGCCTCGCTAATCATGCCCGATGGTGTTACCGGTCGAAAATACCTCGTTTATGGCAATTACCGCGCATTAATGCACTG
GCAGAATACCGATTATTTCGGGATTTCTGTCGCGCATCTGTCCGAGAGAATAAAGTATCCGCCGATAAACTAG

Upstream 100 bases:

>100_bases
TTTACAAATTCCACCACCTCAGGTGAATTTATTGGCAGAGCATAAACTCAGTCTTGGGATCAAAGATTAAGCCAATAGCA
TAAAAATAACAGGAAGGTAT

Downstream 100 bases:

>100_bases
TGTAAAATAGCCCGTATTAAGCGGGCTTTTTATTGAGAAGATACAGTTAATCATGGCATTTTGGCAGAGCAAAACATTAG
CGCAGATGAGCGCAACCGAG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MHRYRAYLTQAYQTNTYPVKVCHFGVYQNKLYRLAAYIFAPIRAFVCFIIWLSACSLPAMAQQSDEKISFSDYLIQLKQE
ARLQGISQTTLDLAFPQIKLFKKARATDSLQTEKTHNLDTYLADTVPEWKVDTARVLFKEHEQQLNAIASQYSVQPRFLV
ALWGLVSDFGEASGDYPVLSVTASLAFSGEREAFFKKEFIAALSIMDKDHIGFQALKSRWNGAMGQTQLMPTDYLAYGQD
GDGDGKKDIWHNTQDAFASAAYLLQQLGWNGNDTWGRQVQVPATLDLAVAGLDKPQSLAQWQTLGIRKFDHTDLPSREDI
NASLIMPDGVTGRKYLVYGNYRALMHWQNTDYFGISVAHLSERIKYPPIN

Sequences:

>Translated_370_residues
MHRYRAYLTQAYQTNTYPVKVCHFGVYQNKLYRLAAYIFAPIRAFVCFIIWLSACSLPAMAQQSDEKISFSDYLIQLKQE
ARLQGISQTTLDLAFPQIKLFKKARATDSLQTEKTHNLDTYLADTVPEWKVDTARVLFKEHEQQLNAIASQYSVQPRFLV
ALWGLVSDFGEASGDYPVLSVTASLAFSGEREAFFKKEFIAALSIMDKDHIGFQALKSRWNGAMGQTQLMPTDYLAYGQD
GDGDGKKDIWHNTQDAFASAAYLLQQLGWNGNDTWGRQVQVPATLDLAVAGLDKPQSLAQWQTLGIRKFDHTDLPSREDI
NASLIMPDGVTGRKYLVYGNYRALMHWQNTDYFGISVAHLSERIKYPPIN
>Mature_370_residues
MHRYRAYLTQAYQTNTYPVKVCHFGVYQNKLYRLAAYIFAPIRAFVCFIIWLSACSLPAMAQQSDEKISFSDYLIQLKQE
ARLQGISQTTLDLAFPQIKLFKKARATDSLQTEKTHNLDTYLADTVPEWKVDTARVLFKEHEQQLNAIASQYSVQPRFLV
ALWGLVSDFGEASGDYPVLSVTASLAFSGEREAFFKKEFIAALSIMDKDHIGFQALKSRWNGAMGQTQLMPTDYLAYGQD
GDGDGKKDIWHNTQDAFASAAYLLQQLGWNGNDTWGRQVQVPATLDLAVAGLDKPQSLAQWQTLGIRKFDHTDLPSREDI
NASLIMPDGVTGRKYLVYGNYRALMHWQNTDYFGISVAHLSERIKYPPIN

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=240, Percent_Identity=27.0833333333333, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 41941; Mature: 41941

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYRAYLTQAYQTNTYPVKVCHFGVYQNKLYRLAAYIFAPIRAFVCFIIWLSACSLPAM
CCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AQQSDEKISFSDYLIQLKQEARLQGISQTTLDLAFPQIKLFKKARATDSLQTEKTHNLDT
HCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHH
YLADTVPEWKVDTARVLFKEHEQQLNAIASQYSVQPRFLVALWGLVSDFGEASGDYPVLS
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEE
VTASLAFSGEREAFFKKEFIAALSIMDKDHIGFQALKSRWNGAMGQTQLMPTDYLAYGQD
EEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHEEECCC
GDGDGKKDIWHNTQDAFASAAYLLQQLGWNGNDTWGRQVQVPATLDLAVAGLDKPQSLAQ
CCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEEEECCCCCHHHHH
WQTLGIRKFDHTDLPSREDINASLIMPDGVTGRKYLVYGNYRALMHWQNTDYFGISVAHL
HHHHCCEECCCCCCCCCCCCCEEEECCCCCCCCEEEEEECEEEEEEECCCCEEEEEHHHH
SERIKYPPIN
HHHCCCCCCC
>Mature Secondary Structure
MHRYRAYLTQAYQTNTYPVKVCHFGVYQNKLYRLAAYIFAPIRAFVCFIIWLSACSLPAM
CCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AQQSDEKISFSDYLIQLKQEARLQGISQTTLDLAFPQIKLFKKARATDSLQTEKTHNLDT
HCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHH
YLADTVPEWKVDTARVLFKEHEQQLNAIASQYSVQPRFLVALWGLVSDFGEASGDYPVLS
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEE
VTASLAFSGEREAFFKKEFIAALSIMDKDHIGFQALKSRWNGAMGQTQLMPTDYLAYGQD
EEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHEEECCC
GDGDGKKDIWHNTQDAFASAAYLLQQLGWNGNDTWGRQVQVPATLDLAVAGLDKPQSLAQ
CCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEEEECCCCCHHHHH
WQTLGIRKFDHTDLPSREDINASLIMPDGVTGRKYLVYGNYRALMHWQNTDYFGISVAHL
HHHHCCEECCCCCCCCCCCCCEEEECCCCCCCCEEEEEECEEEEEEECCCCEEEEEHHHH
SERIKYPPIN
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]