Click here to switch to the map view.

The map label for this gene is rimK [H]

Identifier: 160874997

GI number: 160874997

Start: 2251639

End: 2252625

Strand: Reverse

Name: rimK [H]

Synonym: Sbal195_1882

Alternate gene names: 160874997

Gene position: 2252625-2251639 (Counterclockwise)

Preceding gene: 160874998

Following gene: 160874996

Centisome position: 42.13

GC content: 44.17

Gene sequence:

>987_bases
ATGCGTGGTTGGATTTTATATAAAGAAACCGCGACTCAGCTAAAACCTGAATGGTATGAAATTGAGCGTCTCTTGGCGGC
AGCAAAAGCCGACAACATACAGTTGGAAGTTTACGCACCTGATGAATTTGATCTGACTGTCACCAGAGAAGATAACAAGA
GCATTTTATTGAATGGGCAAGCCGTCGAGCTGCCCGATTTTATTATTCCGCGAATGGGATCAGGCACAACTTACTTTGCC
TTAGCCATTATTCGCCACTTAGAAAGATTAGGGGTGTATTGCCTCAATCCTTCTAAAGCCATTGAAATCGTTAAAGACAA
ATTATTCTCCCAGCAATTGTTGGCAGAGAAAAATTTGCCTACGCCTAAAACTATGTTGGTTAAATTCCCCGTTGATATCG
CCCTTGTAGAGCGCCATCTCGGCTTTCCTGTGGTGATTAAAACGCTTTCTGGCTCACAGGGCAGCGGTGTGTTTTTATCC
CACAAGGCACGTGAATTTGACGATCTAATGCAGTTGATTGAAGCGACCAACAAAAACGCTAACATCATTCTGCAAGAGTT
TATCGCTAACAGCCATGGGCGCGATCTTCGGGTATTTACCATCGGTGGTCGTGTTGCAGGTTGCTACGAAAGGCGTGGTC
AAGAAGACAGCTTTAAAGCCAATGTCAGTGCCGGCGGCTCAGCGCGTCCATTTCAAATCACTCCTGAAATTGAATGGCTT
GCCACGCAAACAGCCAATATTCTCGATCTCGATGTTGCGGGTATCGATTTATTGTTTGATAACGGCCATTACAAAATTTG
CGAAGCAAACTCATCCCCGGGATTTGAAGGCTTAGAATCCTGTTTGAATGTGGATATTGCCGCGCAAATTTTACACTTTA
TTCGCATCCGTTTAGGCATGTTTAATAAAGATGAGCCAAGTAGCCCCAAAATGGTCATTACTGACAATGTAGAAACGTCT
GTGACGTCCGTTATCGAGCCTAAATAG

Upstream 100 bases:

>100_bases
AGCTAGAAGTTGAGGCCGAGGCTGTATTTGATATTGAAGATGATGTTCAGGATGAACCTGAACCCGAGGATAAAGAATGA
AAATTAAGGTTTGCTACTAA

Downstream 100 bases:

>100_bases
CGGACCAGCGATATAATATCAATATCTAGCGGTATCACGCCGCTAGATATTCTTAAATTGATTACTATCCCCCATTTATC
ATGTTTCACATCCATTGGCA

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRGWILYKETATQLKPEWYEIERLLAAAKADNIQLEVYAPDEFDLTVTREDNKSILLNGQAVELPDFIIPRMGSGTTYFA
LAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLPTPKTMLVKFPVDIALVERHLGFPVVIKTLSGSQGSGVFLS
HKAREFDDLMQLIEATNKNANIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGMFNKDEPSSPKMVITDNVETS
VTSVIEPK

Sequences:

>Translated_328_residues
MRGWILYKETATQLKPEWYEIERLLAAAKADNIQLEVYAPDEFDLTVTREDNKSILLNGQAVELPDFIIPRMGSGTTYFA
LAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLPTPKTMLVKFPVDIALVERHLGFPVVIKTLSGSQGSGVFLS
HKAREFDDLMQLIEATNKNANIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGMFNKDEPSSPKMVITDNVETS
VTSVIEPK
>Mature_328_residues
MRGWILYKETATQLKPEWYEIERLLAAAKADNIQLEVYAPDEFDLTVTREDNKSILLNGQAVELPDFIIPRMGSGTTYFA
LAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLPTPKTMLVKFPVDIALVERHLGFPVVIKTLSGSQGSGVFLS
HKAREFDDLMQLIEATNKNANIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGMFNKDEPSSPKMVITDNVETS
VTSVIEPK

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=297, Percent_Identity=27.6094276094276, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI134254462, Length=297, Percent_Identity=29.6296296296296, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1787076, Length=277, Percent_Identity=34.2960288808664, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 36553; Mature: 36553

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGWILYKETATQLKPEWYEIERLLAAAKADNIQLEVYAPDEFDLTVTREDNKSILLNGQ
CCCEEEEECHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCEEEEECC
AVELPDFIIPRMGSGTTYFALAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLP
EEECCHHHCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC
TPKTMLVKFPVDIALVERHLGFPVVIKTLSGSQGSGVFLSHKAREFDDLMQLIEATNKNA
CCCEEEEEECCHHHHHHHHCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCC
NIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
CEEHHHHHCCCCCCEEEEEEECCCEEHHHHHCCCCCCEEEEECCCCCCCCEEECCCEEEE
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGM
EECCCCEEEEEECCEEEEEECCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
FNKDEPSSPKMVITDNVETSVTSVIEPK
CCCCCCCCCCEEEECCCCHHHHHHCCCC
>Mature Secondary Structure
MRGWILYKETATQLKPEWYEIERLLAAAKADNIQLEVYAPDEFDLTVTREDNKSILLNGQ
CCCEEEEECHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCEEEEECC
AVELPDFIIPRMGSGTTYFALAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLP
EEECCHHHCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC
TPKTMLVKFPVDIALVERHLGFPVVIKTLSGSQGSGVFLSHKAREFDDLMQLIEATNKNA
CCCEEEEEECCHHHHHHHHCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCC
NIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
CEEHHHHHCCCCCCEEEEEEECCCEEHHHHHCCCCCCEEEEECCCCCCCCEEECCCEEEE
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGM
EECCCCEEEEEECCEEEEEECCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
FNKDEPSSPKMVITDNVETSVTSVIEPK
CCCCCCCCCCEEEECCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA