Click here to switch to the map view.

The map label for this gene is garR [C]

Identifier: 160874879

GI number: 160874879

Start: 2111586

End: 2112461

Strand: Direct

Name: garR [C]

Synonym: Sbal195_1764

Alternate gene names: 160874879

Gene position: 2111586-2112461 (Clockwise)

Preceding gene: 160874878

Following gene: 160874881

Centisome position: 39.49

GC content: 51.03

Gene sequence:

>876_bases
ATGGCAAAAGTCGCATTTATTGGTTTAGGCGTAATGGGATATCCCATGGCGAGGCATTTATTGAACAAAGGCCATGAGGT
CACTGTTTATAATCGCACTTTTGCCAAGGCACAAACTTGGGTCGACACCTATGGCGGACGCTGCTGCCCAACGCCAAAGG
AAGCGGCAGAGGGTCAAGATATTGTGTTTACTTGCGTGGGCAATGATAACGATCTGCGTGAAGTGGTATTAGGCAGTGAT
GGCGTTATTCATGGGATGAAAAAGGGCGCAGTGCTGGTTGACCATACTACGGCTTCGGCCGATGTCGCTCGTGAGCTTCA
TCAAGTGTTAGCGGAAAAGGGCATTGATTTCCTCGATGCGCCTGTGTCGGGCGGTCAAGCGGGCGCCGAAAATGGCGCCT
TGACTGTGATGGTTGGCGGCGATGAAGCTGTGTTTGCCCGTGTTAAACCTGTGATTGAAGCCTTTGCCCGCTGCGCCGAG
CGTTTAGGTGAGGTTGGTGCAGGTCAGTTAACTAAGATGGTGAACCAAATCTGTATCGCGGGCGTGGTGCAAGGTTTAGC
TGAAGCATTACAGTTTGCGCGCAAGGCAGGACTCGATGGCGAGAAAGTCATTGAAGTGATTAGCAAAGGCGCGGCGCAAA
GCTGGCAAATGGAAAATCGCTACAAGACGATGTGGGGCCAAAGCTATGATTTTGGTTTTGCCGTCGATTGGATGCGTAAA
GATTTAGGCATCGCCTTAGAAGAAGCTCGCCGCAATGGTTCACACTTGCCATTAACCGCGTTAGTCGATCAATTCTATTC
AGAAGTACAGGGCATGGGCGGTAACCGTTGGGATACGTCTAGCCTGTTAGCGCGCCTCGAGAAATCGGCGAAATAA

Upstream 100 bases:

>100_bases
TTGTTGAGGGTTTAGCGGTAACATATTGGGCAGACGAAATACAACTCGATTCAGCAATTTCTACTTGATTCACCTTGTTT
ACCTATTTGAGGATCACTTC

Downstream 100 bases:

>100_bases
GCTTATAAAACTTAGATTTTGCACTGATAAAAATGCCCAGTCCCGATTGCGTTGGCAAATCGGACTGGGCATTTTTGTGT
GTATCGTTTTTGACTAGGAT

Product: NAD-binding 6-phosphogluconate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVDTYGGRCCPTPKEAAEGQDIVFTCVGNDNDLREVVLGSD
GVIHGMKKGAVLVDHTTASADVARELHQVLAEKGIDFLDAPVSGGQAGAENGALTVMVGGDEAVFARVKPVIEAFARCAE
RLGEVGAGQLTKMVNQICIAGVVQGLAEALQFARKAGLDGEKVIEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRK
DLGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLLARLEKSAK

Sequences:

>Translated_291_residues
MAKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVDTYGGRCCPTPKEAAEGQDIVFTCVGNDNDLREVVLGSD
GVIHGMKKGAVLVDHTTASADVARELHQVLAEKGIDFLDAPVSGGQAGAENGALTVMVGGDEAVFARVKPVIEAFARCAE
RLGEVGAGQLTKMVNQICIAGVVQGLAEALQFARKAGLDGEKVIEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRK
DLGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLLARLEKSAK
>Mature_290_residues
AKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVDTYGGRCCPTPKEAAEGQDIVFTCVGNDNDLREVVLGSDG
VIHGMKKGAVLVDHTTASADVARELHQVLAEKGIDFLDAPVSGGQAGAENGALTVMVGGDEAVFARVKPVIEAFARCAER
LGEVGAGQLTKMVNQICIAGVVQGLAEALQFARKAGLDGEKVIEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRKD
LGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLLARLEKSAK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=31.3993174061433, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=28.5714285714286, Blast_Score=144, Evalue=9e-35,
Organism=Escherichia coli, GI145693186, Length=289, Percent_Identity=38.4083044982699, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=36.3321799307958, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1790315, Length=295, Percent_Identity=29.8305084745763, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1789092, Length=295, Percent_Identity=33.2203389830508, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788341, Length=302, Percent_Identity=25.8278145695364, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=27.6363636363636, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24655230, Length=298, Percent_Identity=31.5436241610738, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI19922568, Length=298, Percent_Identity=31.5436241610738, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI28574115, Length=281, Percent_Identity=24.5551601423488, Blast_Score=117, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31323; Mature: 31192

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVDTYGGRCCPTPKEAAEGQD
CCEEEEECCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCHHHCCCCC
IVFTCVGNDNDLREVVLGSDGVIHGMKKGAVLVDHTTASADVARELHQVLAEKGIDFLDA
EEEEEECCCCCHHHHHCCCCCCEEHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEECC
PVSGGQAGAENGALTVMVGGDEAVFARVKPVIEAFARCAERLGEVGAGQLTKMVNQICIA
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GVVQGLAEALQFARKAGLDGEKVIEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DLGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLLARLEKSAK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AKVAFIGLGVMGYPMARHLLNKGHEVTVYNRTFAKAQTWVDTYGGRCCPTPKEAAEGQD
CEEEEECCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCHHHCCCCC
IVFTCVGNDNDLREVVLGSDGVIHGMKKGAVLVDHTTASADVARELHQVLAEKGIDFLDA
EEEEEECCCCCHHHHHCCCCCCEEHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEECC
PVSGGQAGAENGALTVMVGGDEAVFARVKPVIEAFARCAERLGEVGAGQLTKMVNQICIA
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GVVQGLAEALQFARKAGLDGEKVIEVISKGAAQSWQMENRYKTMWGQSYDFGFAVDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DLGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLLARLEKSAK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA