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The map label for this gene is sbcB [H]

Identifier: 160874834

GI number: 160874834

Start: 2065304

End: 2066722

Strand: Reverse

Name: sbcB [H]

Synonym: Sbal195_1717

Alternate gene names: 160874834

Gene position: 2066722-2065304 (Counterclockwise)

Preceding gene: 160874836

Following gene: 160874833

Centisome position: 38.65

GC content: 44.75

Gene sequence:

>1419_bases
ATGAAGATGAAAGACGATAAAGCAGGGTCGATATTTTGGCACGACTACGAAACCTTCGGGGCTAACCCGGCGAAAGACAG
ACCCTCGCAATTTGCCGGTATTCGCACGGATTTAGATCTCAATATTATCGGCGAGCCTGAGACATTTTATTGCAAGCAAG
CCCCTGATTATTTGCCGTCACCAGAAGCGATATTGATCACAGGGATCACGCCGCAGTTAGCCAATTTAAAAGGAGTCCCT
GAAACTGAGTTTATGGGGCGTATTCAAGCACTGTTTAGCCAGCCAAACACCTGTGTCGCGGGCTACAACTCACTGCGTTT
TGATGATGAAGTGACCCGTTACGGCTTTTATAGAAATTTTATCGATCCCTATGCCCGTGAATGGCAAAATGGTAACTCTC
GTTGGGATATCATTGATTTAGTGCGCGCGTGTTACGCATTTAGACCCGAAGGGATCAATTGGCCAACCAAGGAAGATGGA
TCTCCTAGTTTTAAGCTAGAACATTTGACCCAAGCGAATGGTTTAAGCCATGAGAAAGCCCATGATGCCATGTCTGATGT
GTATGCAACCATCGCTATGGCAAAACTCATTCGCGAGAAACAACCTAAGTTATATCAGTACTATTTTGACCTGCGCCGTA
AGCAAGCGGTGAGCGATCAAATCGATGTGCTCAATATGCAGCCGTTAGTACACGTGAGTTCTAAGATCAGTGCGCGCAAT
GGTTGTACGACGTTGATTTCCACCGTGGCGCACCATCCAAACAATAAAAATGCGGTTATTTGCGTTAATTTAGCCATGGA
TTTAACGCCACTCTTTGAGCTAGATATCGAGCAAATTAAAACGCGTATGTATACGCCAAGAGCCGATCTTGCCGAAGACG
AATTACCGATCGCGGTAAAACAAATTCATTTAAATAAGTGCCCTTTTATCACTTCGGCTAAAATCCTCAGCGATGAACAT
GCCGCTCGTTTAGGCATAGATAAAGAATTTGCCAGAGAGCAATATAAACGCCTTAAACTTCACCCAGAACTACGTGAAAA
ACTGGCGCTATTATTTGAGCACGATGACAGCAGAGCGACCACTGATCCCGATTTGATGCTGTATTCTGGTGGTTTCTTTA
GCCAAGCTGATAAAGCGAAAATGGAAATCATCCGTCATACCCTACCGCAAAATTTAGCGGCGCTAGACTTGCAGTTTGAT
GACCCACGTATCCCTGAAATGCTGTTTCGTTACCGTGCCCGTAATTACCCTGAACTACTCGATTACGAAGAGTCACAGCG
CTGGCGTGAATTCTGTCAAAATCGCTTAGCCGATCAAGACTACGTACTTAAATTAGAAAATCTGCTGCAAGATACACAAG
CGGATGAGACTAAACAGAAGTTACTGGCTGCCCTCTGTCATTATTTGCGTGAACTTTAA

Upstream 100 bases:

>100_bases
CGAAGAGTAGCAAACTAAATTGATGTGAATGACAACGAATCCAAATAAAGTCATTTGACGTTAAATAACATGATGTTAAA
TGCTTTAGAGAACGAAATAA

Downstream 100 bases:

>100_bases
GTTTAATCAATTCAAGCTTAAGTCTTTTATTTAATAGTCTTAAGCTTGTTTATATTCGTGAATAGACTCACTCTATTAGG
CGCATAACTTAAATTGTTTA

Product: exonuclease I

Products: NA

Alternate protein names: Exonuclease I; DNA deoxyribophosphodiesterase; dRPase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKMKDDKAGSIFWHDYETFGANPAKDRPSQFAGIRTDLDLNIIGEPETFYCKQAPDYLPSPEAILITGITPQLANLKGVP
ETEFMGRIQALFSQPNTCVAGYNSLRFDDEVTRYGFYRNFIDPYAREWQNGNSRWDIIDLVRACYAFRPEGINWPTKEDG
SPSFKLEHLTQANGLSHEKAHDAMSDVYATIAMAKLIREKQPKLYQYYFDLRRKQAVSDQIDVLNMQPLVHVSSKISARN
GCTTLISTVAHHPNNKNAVICVNLAMDLTPLFELDIEQIKTRMYTPRADLAEDELPIAVKQIHLNKCPFITSAKILSDEH
AARLGIDKEFAREQYKRLKLHPELREKLALLFEHDDSRATTDPDLMLYSGGFFSQADKAKMEIIRHTLPQNLAALDLQFD
DPRIPEMLFRYRARNYPELLDYEESQRWREFCQNRLADQDYVLKLENLLQDTQADETKQKLLAALCHYLREL

Sequences:

>Translated_472_residues
MKMKDDKAGSIFWHDYETFGANPAKDRPSQFAGIRTDLDLNIIGEPETFYCKQAPDYLPSPEAILITGITPQLANLKGVP
ETEFMGRIQALFSQPNTCVAGYNSLRFDDEVTRYGFYRNFIDPYAREWQNGNSRWDIIDLVRACYAFRPEGINWPTKEDG
SPSFKLEHLTQANGLSHEKAHDAMSDVYATIAMAKLIREKQPKLYQYYFDLRRKQAVSDQIDVLNMQPLVHVSSKISARN
GCTTLISTVAHHPNNKNAVICVNLAMDLTPLFELDIEQIKTRMYTPRADLAEDELPIAVKQIHLNKCPFITSAKILSDEH
AARLGIDKEFAREQYKRLKLHPELREKLALLFEHDDSRATTDPDLMLYSGGFFSQADKAKMEIIRHTLPQNLAALDLQFD
DPRIPEMLFRYRARNYPELLDYEESQRWREFCQNRLADQDYVLKLENLLQDTQADETKQKLLAALCHYLREL
>Mature_472_residues
MKMKDDKAGSIFWHDYETFGANPAKDRPSQFAGIRTDLDLNIIGEPETFYCKQAPDYLPSPEAILITGITPQLANLKGVP
ETEFMGRIQALFSQPNTCVAGYNSLRFDDEVTRYGFYRNFIDPYAREWQNGNSRWDIIDLVRACYAFRPEGINWPTKEDG
SPSFKLEHLTQANGLSHEKAHDAMSDVYATIAMAKLIREKQPKLYQYYFDLRRKQAVSDQIDVLNMQPLVHVSSKISARN
GCTTLISTVAHHPNNKNAVICVNLAMDLTPLFELDIEQIKTRMYTPRADLAEDELPIAVKQIHLNKCPFITSAKILSDEH
AARLGIDKEFAREQYKRLKLHPELREKLALLFEHDDSRATTDPDLMLYSGGFFSQADKAKMEIIRHTLPQNLAALDLQFD
DPRIPEMLFRYRARNYPELLDYEESQRWREFCQNRLADQDYVLKLENLLQDTQADETKQKLLAALCHYLREL

Specific function: Also functions as a DNA deoxyribophosphodiesterase that releases deoxyribose-phosphate moieties following the cleavage DNA at an apurinic/apyrimidinic (AP) site by either an AP endonuclease AP lyase [H]

COG id: COG2925

COG function: function code L; Exonuclease I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788321, Length=475, Percent_Identity=52.6315789473684, Blast_Score=499, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013620
- InterPro:   IPR013520
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T; PF08411 Exonuc_X-T_C [H]

EC number: =3.1.11.1 [H]

Molecular weight: Translated: 54495; Mature: 54495

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKDDKAGSIFWHDYETFGANPAKDRPSQFAGIRTDLDLNIIGEPETFYCKQAPDYLPS
CCCCCCCCCCEEEECHHHCCCCCCCCCCHHHCCCEECCEEEEECCCCCEEECCCCCCCCC
PEAILITGITPQLANLKGVPETEFMGRIQALFSQPNTCVAGYNSLRFDDEVTRYGFYRNF
CCEEEEECCCHHHHCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHH
IDPYAREWQNGNSRWDIIDLVRACYAFRPEGINWPTKEDGSPSFKLEHLTQANGLSHEKA
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCHHHH
HDAMSDVYATIAMAKLIREKQPKLYQYYFDLRRKQAVSDQIDVLNMQPLVHVSSKISARN
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
GCTTLISTVAHHPNNKNAVICVNLAMDLTPLFELDIEQIKTRMYTPRADLAEDELPIAVK
CHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
QIHLNKCPFITSAKILSDEHAARLGIDKEFAREQYKRLKLHPELREKLALLFEHDDSRAT
HHCCCCCCCCCHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
TDPDLMLYSGGFFSQADKAKMEIIRHTLPQNLAALDLQFDDPRIPEMLFRYRARNYPELL
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHH
DYEESQRWREFCQNRLADQDYVLKLENLLQDTQADETKQKLLAALCHYLREL
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKMKDDKAGSIFWHDYETFGANPAKDRPSQFAGIRTDLDLNIIGEPETFYCKQAPDYLPS
CCCCCCCCCCEEEECHHHCCCCCCCCCCHHHCCCEECCEEEEECCCCCEEECCCCCCCCC
PEAILITGITPQLANLKGVPETEFMGRIQALFSQPNTCVAGYNSLRFDDEVTRYGFYRNF
CCEEEEECCCHHHHCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHH
IDPYAREWQNGNSRWDIIDLVRACYAFRPEGINWPTKEDGSPSFKLEHLTQANGLSHEKA
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCHHHH
HDAMSDVYATIAMAKLIREKQPKLYQYYFDLRRKQAVSDQIDVLNMQPLVHVSSKISARN
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
GCTTLISTVAHHPNNKNAVICVNLAMDLTPLFELDIEQIKTRMYTPRADLAEDELPIAVK
CHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
QIHLNKCPFITSAKILSDEHAARLGIDKEFAREQYKRLKLHPELREKLALLFEHDDSRAT
HHCCCCCCCCCHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
TDPDLMLYSGGFFSQADKAKMEIIRHTLPQNLAALDLQFDDPRIPEMLFRYRARNYPELL
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHH
DYEESQRWREFCQNRLADQDYVLKLENLLQDTQADETKQKLLAALCHYLREL
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3539937; 9097040; 9278503; 1329027 [H]