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The map label for this gene is folD [H]

Identifier: 160874742

GI number: 160874742

Start: 1963384

End: 1964238

Strand: Reverse

Name: folD [H]

Synonym: Sbal195_1625

Alternate gene names: 160874742

Gene position: 1964238-1963384 (Counterclockwise)

Preceding gene: 160874749

Following gene: 160874740

Centisome position: 36.73

GC content: 49.71

Gene sequence:

>855_bases
ATGACAGCCCAAATAATCGACGGCAAGGCGATTGCTCAATCCATCCGCACTAAGCTCAGCGAAAAAGTCACCGCCCGCAA
AGAGGCTGGACAGCGCATTCCGGGTTTAGCCGTAGTATTAGTGGGAGCCGATCCGGCATCACAGGTTTATGTTGGAAGCA
AACGTAAGGCCTGTGAGGAAGTTGGATTTATTTCTCGTTCATACGATTTAGAAACAAGTTGCAGTGAAGATGAGTTGCTG
AGCCTTATTGATAGTCTGAATGAAGATCCGACCATCGATGGCATTTTAGTGCAACTGCCACTACCCGCCCATATCGAAGA
TTCAAAAGTCATCGAACGTATTCGCCCAGATAAAGATGTCGATGGATTTCACCCGTACAACGTTGGCCGTTTAGCCCAGC
GTATTCCTGTGCTGCGCTCTTGCACGCCGATGGGCATAATGACACTCATCAAATCCACCGGCGTTGATACCTATGGTTTA
GATGCTGTCGTGGTTGGTGCGTCTAATATTGTCGGCCGCCCTATGACGTTAGAATTACTGTTGGCGGGTTGCACTACGAC
CACTTGCCACCGCTTCACGAAGAATCTTGAGCAAAAAATTCGTATCGCAGATCTCGTGGTTGTCGCAGTCGGTAAACCCG
GCTTCATTCCTGGTGAGTGGATCAAGCCCGGCGCTATCGTCATTGACGTAGGCATTAACCGTTTAGATAACGGTACCTTA
GTCGGCGATGTGCAATATGACGTGGCGGCGCAAAATGCCAGCTTTATCACCCCAGTCCCCGGCGGTGTAGGCCCGATGAC
AATTGCGAGTCTATTGGAAAACACCTTATACGCTGCCGAGCAATATCACGACTAA

Upstream 100 bases:

>100_bases
CATGACACAGTAGCTGTGCCCTGTTAAAATAGCCGCGATTGCCAATCTCTGTGTTAACTACTGTTTTAACCATCGCCCAT
ATAAAGGATATCCTACACCC

Downstream 100 bases:

>100_bases
GTTCTAAGTTCTAAGTTCTAAGTTCTAAGTTCTAAGTTCTAAGTTCTAAGTTCTAAGTTCCAAGTTCTAAGTTCTGCAAG
CATAAAAAACCTGCATGATG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTAQIIDGKAIAQSIRTKLSEKVTARKEAGQRIPGLAVVLVGADPASQVYVGSKRKACEEVGFISRSYDLETSCSEDELL
SLIDSLNEDPTIDGILVQLPLPAHIEDSKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGL
DAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKNLEQKIRIADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLDNGTL
VGDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD

Sequences:

>Translated_284_residues
MTAQIIDGKAIAQSIRTKLSEKVTARKEAGQRIPGLAVVLVGADPASQVYVGSKRKACEEVGFISRSYDLETSCSEDELL
SLIDSLNEDPTIDGILVQLPLPAHIEDSKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGL
DAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKNLEQKIRIADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLDNGTL
VGDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD
>Mature_283_residues
TAQIIDGKAIAQSIRTKLSEKVTARKEAGQRIPGLAVVLVGADPASQVYVGSKRKACEEVGFISRSYDLETSCSEDELLS
LIDSLNEDPTIDGILVQLPLPAHIEDSKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGLD
AVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKNLEQKIRIADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLDNGTLV
GDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=298, Percent_Identity=46.6442953020134, Blast_Score=235, Evalue=3e-62,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=42.1768707482993, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=43.1972789115646, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI36796743, Length=220, Percent_Identity=28.1818181818182, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=71.4788732394366, Blast_Score=425, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17568735, Length=294, Percent_Identity=39.4557823129252, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=41.2751677852349, Blast_Score=229, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=41.8118466898955, Blast_Score=218, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6322933, Length=311, Percent_Identity=26.6881028938907, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=47.8114478114478, Blast_Score=257, Evalue=7e-69,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=47.8114478114478, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=45.1724137931035, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=45.1724137931035, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=45.1724137931035, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=45.1724137931035, Blast_Score=228, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30449; Mature: 30318

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQIIDGKAIAQSIRTKLSEKVTARKEAGQRIPGLAVVLVGADPASQVYVGSKRKACEE
CCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFISRSYDLETSCSEDELLSLIDSLNEDPTIDGILVQLPLPAHIEDSKVIERIRPDKDV
HHCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGLDAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCCHHHCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKNLEQKIRIADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLDNGTL
HHCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEE
VGDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD
EEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAQIIDGKAIAQSIRTKLSEKVTARKEAGQRIPGLAVVLVGADPASQVYVGSKRKACEE
CCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFISRSYDLETSCSEDELLSLIDSLNEDPTIDGILVQLPLPAHIEDSKVIERIRPDKDV
HHCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGLDAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCCHHHCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKNLEQKIRIADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLDNGTL
HHCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEE
VGDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD
EEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA