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The map label for this gene is thiI

Identifier: 160874505

GI number: 160874505

Start: 1660653

End: 1662107

Strand: Direct

Name: thiI

Synonym: Sbal195_1387

Alternate gene names: 160874505

Gene position: 1660653-1662107 (Clockwise)

Preceding gene: 160874504

Following gene: 160874507

Centisome position: 31.06

GC content: 45.77

Gene sequence:

>1455_bases
ATGAAGTTTATTGTAAAGCTGTACCCAGAAATCATGATGAAGAGCAAACCCGTAAGGATGCGCTTCACCAAAATGCTCGA
AACCAACATACGTAATGTACTCAAAAAAGTCGACGAAGACGCTAAAGTTCAACGTCAGTGGGACCGCATTATGGTAATGG
TGCCAAAGGATAAGCCTGAATTGGCCCAAGCCTTCGGTGAGCGTCTGGCGTGTATTCCTGGCATTGCACACGTAGTGCAA
GTGGATGAGTACAGCTTTGAATCTGTGGACGATATTTATCAGCAAGTCTTGCCTGTTTACCGTGACCAACTCGCGGGTAA
AACCTTCTGCGTGCGAGTGAAGCGCACTGGCGATCACGATTTTAATTCTATCGAAGTGGAGCGTTACGTCGGCGGCGGCT
TAAATCAGTTTACCGATGCTTTAGGTGTGCGTTTAAAGAATCCAGATATTACCGTTAACCTTGAAATTGAACGCGATAAT
CTTTACATGGTCACTAAGCGTATTGAAGGCTTAGGTGGTTTCCCGATGGCGACGCAGGAAGATGTGTTGTCTTTGATTTC
AGGTGGTTTTGACTCTGGTGTGTCTAGCTATCAATTTATCAAAAAGGGCGCGCGTACCCATTACTGCTTCTTCAATCTGG
GCGGCGCGCAGCATGAAATTGGCGTAAAACAAGTGGCTTACCATTTGTGGAAAACCTACGGCGAATCCCACAAGGTTAAG
TTTATCTCTGTGCCTTTTGAGCCTGTGGTCGCTGAGATTTTAGAGCGTATCGATAATGGCCAAATGGGCGTTGTGCTCAA
GCGTATGATGATGCGCACCGCGGCGCGTATTGCCGACCGTATGGGCATTCAAGCCTTAGTAACCGGTGAAAGTTTAGGCC
AAGTATCGAGCCAAACGCTGACCAACTTAAACGTGATCGACCGCTGTACTGAGCTGTTGATTTTACGTCCGCTGATCGCC
ATGGATAAGCAAGACATTATCAACGAGAGCCGTAAAATCGGCACCGAAGATTTTGCCAAGTCGATGCCTGAATATTGCGG
CGTGATTTCGCAAAAGCCAACAGTTAAAGCGGTATTAGCTAAAGTTGAAGCGGAAGAGAAGAAGTTCTCTGAAGATTTGA
TCGATCAAATCATCGCCCAGTCTGTCACTATTGATATCAGGGAGATCGCAGAACAAATGGATACTCGGATTACTGAGACT
GAAACGGTCGCCAGTATTGATACCAACCAAGTGGTCATTGATATTCGCGCCCCTGAAGAAGAGGAAAGCAAGCCGTTGCA
AATTGAAGGTATTGAGATTAAACGTATCCCATTCTTCAAGTTAGCGACTCAATTTGCCGATCTCGATAAGCAAAAAACCT
ATTTGCTGTATTGTGAGCGTGGGGTGATGAGTAAATTGCAGGCATTGTATTTAATCGAGCAAGGCTACACTAACGTCAAA
GTGTATCGCCCATAA

Upstream 100 bases:

>100_bases
ATGCGCGCTGAAACTCGCATTTTTAGGTCGCTTGGGTATATAATCCCCCGATTATTCTTTTAACCAATTTTGCTGTTTTA
AAATTGCCCGCGGAAATCCC

Downstream 100 bases:

>100_bases
ATCCATGCAAACTTGCCCACTGACATGTCTTGTCAGTGGGAAGTGCCCATAGAGATTGAATACAAAAAAGCCGCATATGA
ATGCGGCTTTTTCTTTTGGT

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MKFIVKLYPEIMMKSKPVRMRFTKMLETNIRNVLKKVDEDAKVQRQWDRIMVMVPKDKPELAQAFGERLACIPGIAHVVQ
VDEYSFESVDDIYQQVLPVYRDQLAGKTFCVRVKRTGDHDFNSIEVERYVGGGLNQFTDALGVRLKNPDITVNLEIERDN
LYMVTKRIEGLGGFPMATQEDVLSLISGGFDSGVSSYQFIKKGARTHYCFFNLGGAQHEIGVKQVAYHLWKTYGESHKVK
FISVPFEPVVAEILERIDNGQMGVVLKRMMMRTAARIADRMGIQALVTGESLGQVSSQTLTNLNVIDRCTELLILRPLIA
MDKQDIINESRKIGTEDFAKSMPEYCGVISQKPTVKAVLAKVEAEEKKFSEDLIDQIIAQSVTIDIREIAEQMDTRITET
ETVASIDTNQVVIDIRAPEEEESKPLQIEGIEIKRIPFFKLATQFADLDKQKTYLLYCERGVMSKLQALYLIEQGYTNVK
VYRP

Sequences:

>Translated_484_residues
MKFIVKLYPEIMMKSKPVRMRFTKMLETNIRNVLKKVDEDAKVQRQWDRIMVMVPKDKPELAQAFGERLACIPGIAHVVQ
VDEYSFESVDDIYQQVLPVYRDQLAGKTFCVRVKRTGDHDFNSIEVERYVGGGLNQFTDALGVRLKNPDITVNLEIERDN
LYMVTKRIEGLGGFPMATQEDVLSLISGGFDSGVSSYQFIKKGARTHYCFFNLGGAQHEIGVKQVAYHLWKTYGESHKVK
FISVPFEPVVAEILERIDNGQMGVVLKRMMMRTAARIADRMGIQALVTGESLGQVSSQTLTNLNVIDRCTELLILRPLIA
MDKQDIINESRKIGTEDFAKSMPEYCGVISQKPTVKAVLAKVEAEEKKFSEDLIDQIIAQSVTIDIREIAEQMDTRITET
ETVASIDTNQVVIDIRAPEEEESKPLQIEGIEIKRIPFFKLATQFADLDKQKTYLLYCERGVMSKLQALYLIEQGYTNVK
VYRP
>Mature_484_residues
MKFIVKLYPEIMMKSKPVRMRFTKMLETNIRNVLKKVDEDAKVQRQWDRIMVMVPKDKPELAQAFGERLACIPGIAHVVQ
VDEYSFESVDDIYQQVLPVYRDQLAGKTFCVRVKRTGDHDFNSIEVERYVGGGLNQFTDALGVRLKNPDITVNLEIERDN
LYMVTKRIEGLGGFPMATQEDVLSLISGGFDSGVSSYQFIKKGARTHYCFFNLGGAQHEIGVKQVAYHLWKTYGESHKVK
FISVPFEPVVAEILERIDNGQMGVVLKRMMMRTAARIADRMGIQALVTGESLGQVSSQTLTNLNVIDRCTELLILRPLIA
MDKQDIINESRKIGTEDFAKSMPEYCGVISQKPTVKAVLAKVEAEEKKFSEDLIDQIIAQSVTIDIREIAEQMDTRITET
ETVASIDTNQVVIDIRAPEEEESKPLQIEGIEIKRIPFFKLATQFADLDKQKTYLLYCERGVMSKLQALYLIEQGYTNVK
VYRP

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=484, Percent_Identity=60.5371900826446, Blast_Score=622, Evalue=1e-179,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_SHEB2 (B8EAU2)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002358907.1
- ProteinModelPortal:   B8EAU2
- GeneID:   7089066
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_2998
- HOGENOM:   HBG646072
- OMA:   GYPIGTQ
- ProtClustDB:   PRK01269
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.250.10
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP; SSF52821 Rhodanese-like

EC number: =2.8.1.4

Molecular weight: Translated: 55048; Mature: 55048

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50206 RHODANESE_3; PS51165 THUMP

Important sites: ACT_SITE 458-458 BINDING 267-267 BINDING 289-289 BINDING 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIVKLYPEIMMKSKPVRMRFTKMLETNIRNVLKKVDEDAKVQRQWDRIMVMVPKDKPE
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEEECCCCHH
LAQAFGERLACIPGIAHVVQVDEYSFESVDDIYQQVLPVYRDQLAGKTFCVRVKRTGDHD
HHHHHHHHHHHHCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
FNSIEVERYVGGGLNQFTDALGVRLKNPDITVNLEIERDNLYMVTKRIEGLGGFPMATQE
CCCEEEHHHHCCCHHHHHHHHCCEECCCCEEEEEEEECCCEEEEEEHHCCCCCCCCCCHH
DVLSLISGGFDSGVSSYQFIKKGARTHYCFFNLGGAQHEIGVKQVAYHLWKTYGESHKVK
HHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
FISVPFEPVVAEILERIDNGQMGVVLKRMMMRTAARIADRMGIQALVTGESLGQVSSQTL
EEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
TNLNVIDRCTELLILRPLIAMDKQDIINESRKIGTEDFAKSMPEYCGVISQKPTVKAVLA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCHHHHCCCCCCCHHHHHHH
KVEAEEKKFSEDLIDQIIAQSVTIDIREIAEQMDTRITETETVASIDTNQVVIDIRAPEE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHEEECCCEEEEEEECCCC
EESKPLQIEGIEIKRIPFFKLATQFADLDKQKTYLLYCERGVMSKLQALYLIEQGYTNVK
CCCCCEEEECEEEECCCHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEE
VYRP
EECC
>Mature Secondary Structure
MKFIVKLYPEIMMKSKPVRMRFTKMLETNIRNVLKKVDEDAKVQRQWDRIMVMVPKDKPE
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEEECCCCHH
LAQAFGERLACIPGIAHVVQVDEYSFESVDDIYQQVLPVYRDQLAGKTFCVRVKRTGDHD
HHHHHHHHHHHHCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
FNSIEVERYVGGGLNQFTDALGVRLKNPDITVNLEIERDNLYMVTKRIEGLGGFPMATQE
CCCEEEHHHHCCCHHHHHHHHCCEECCCCEEEEEEEECCCEEEEEEHHCCCCCCCCCCHH
DVLSLISGGFDSGVSSYQFIKKGARTHYCFFNLGGAQHEIGVKQVAYHLWKTYGESHKVK
HHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
FISVPFEPVVAEILERIDNGQMGVVLKRMMMRTAARIADRMGIQALVTGESLGQVSSQTL
EEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
TNLNVIDRCTELLILRPLIAMDKQDIINESRKIGTEDFAKSMPEYCGVISQKPTVKAVLA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCHHHHCCCCCCCHHHHHHH
KVEAEEKKFSEDLIDQIIAQSVTIDIREIAEQMDTRITETETVASIDTNQVVIDIRAPEE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHEEECCCEEEEEEECCCC
EESKPLQIEGIEIKRIPFFKLATQFADLDKQKTYLLYCERGVMSKLQALYLIEQGYTNVK
CCCCCEEEECEEEECCCHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEE
VYRP
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA