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The map label for this gene is ycdT [H]

Identifier: 160874459

GI number: 160874459

Start: 1591295

End: 1593040

Strand: Direct

Name: ycdT [H]

Synonym: Sbal195_1340

Alternate gene names: 160874459

Gene position: 1591295-1593040 (Clockwise)

Preceding gene: 160874458

Following gene: 160874460

Centisome position: 29.76

GC content: 41.29

Gene sequence:

>1746_bases
ATGCCCTTAATGGCTGCAACTATCAATTTTGATGAGGTATTAGCTGATCTGGAGCAGCTACAACATACCGATATGCAAAG
TGCAATCACTAAGCTCCATGAACTTGAAGCTGATTTCGAGCATATGACGCGTTTTCAGCAAGGTCGTTTATTGGTATTTA
AAGGGACTTCATTTATTTATTCAGGTCAATATCAAGAAGCCCTAGATAATTTAATTCAATCTGAAAATTTAGTTCAAAAT
AGCGATTATTTATTTTCTGCCTATAGCTATGAGGCAACTGCGTACATCGCCCTACGGAACTTTAAAGACGCTTTTGTTGT
AATGAGCAAAAGCCTAGGGCTAATAGATCGAATAGATAATATAGATATCAAACGTGCTGCATATCTACGTTTAGCCAGTT
TGTACTCTGCAATGGGCATAAAAGAAGAGGTGGGAACCTACGCGGCTAAGGCAGAATCGCTTGCTTCAGCAGATAATGTA
AAAGATATCTGTACTGCAAAGTTGTATTTATCAGTTTATCAATTAGAGCTAGAGGCCTTTACTAAGGCGTTTGATGAATT
CCAGTACACACACGATTTTTGTGAGTCTAATGGCTTCCCATTGATTTCGTTTATTGCATTGAAAGGGATGGGTGAGTCTA
AGTTAAGGTTGCAAGAATACTCTGCTGCAGTTGCTTTACTTTTGAAAGCATTAAAGGGATATGAGTCTTTTAATTTTCAA
CTAGAAATTAATAGTGTCCATTCTTTGTTAACCGAAGCGTATTTTGCACTAAAGCAATCTGCTGAGGTGGTCGAGCATGC
CAAGTTCGTTACGTCATTACCTAATGATCCTAGTTATACCGAGTTAAAACATACTGTCTTTAAAGTGTTAGCGGGCTTAA
ATGCTGAGAATAAACAGTTTGAACAAGCCTATGAATATTCGAAAAAAGAGCAGTATTACAATCAGCTGATGTTTGATGAA
GCGAAAATGAAAACGTTAGCGTATCAGGCGGCAAAGTTTAATGCTGATGAACAGTCACGTGAGATTAATTTATTGAATAA
GGAACGTGAACTGTATGTCGCTCAGCAAACGGTAAAAGAGCGCGAATACACCAATATGCTGATGTTTGTCACTATTCTAG
TAGGCGGACTGTTCTTCCTCGGGATTTTGTTAGCTGTAGGCCAGATGCAGAAACGCAAGTTTATGCGTATGGCGAGAATG
GATGCATTAACAGGCATCTTGAATCGCGGTGCAGGGCAAGAGTTAGGGGAAAACCTTTTTGTGCAAGCCACGGCCCGCGG
CGGGGATTTTTGTGTCATTTTATTCGATTTAGATTTCTTTAAACGCATCAACGATTCTTTTGGTCATGGCACTGGCGATT
GGGCGCTTAAAAAGGTGGTTGATTCCCTTAAATCTCATATCCGAAATGGTGATGTTTTTGCTCGTATCGGCGGTGAGGAG
TTTGCGATTTTATTACCCTATGCCGATGAAGAGAAAGGTATGGAAGTGGCAGAGCAGTGCCGAGCCCGTATCGAGGCCAT
TGATACCCAACATTCAGGACATAAGTACGTCATTACGGCGAGTTTTGGCGTGAGCGGTTTAATGGCTGATGATCTGAGTT
TAGACCCATTATTTCACCGAGCCGACATGGCGCTTTATGCCGCTAAGGCCAATGGCCGCAATGCCGTAGTACGCTATTCT
GACATCTTAAAATGTGACAAGCAGGCAACTGATTTACGGCGTCATATCGCATCGCCCTCTGTGTAA

Upstream 100 bases:

>100_bases
GGATGATGATTGTAAAGCCTACTTAGTTATTTCCCATGGTAATGGTGAGTAATTGAAACTCCTGCGCGCTTTTTTGTGGT
TATGTTCGGGCATATTTTGT

Downstream 100 bases:

>100_bases
TGTAAGTCGAGTGAACATTTAGTCTCATCCTGAGTTGGCAAAGTCCTTACTGGTTTTTATCGTTGCTTCATTTGTTTCTC
GTTTTGCGTTTTGCCGCCAC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MPLMAATINFDEVLADLEQLQHTDMQSAITKLHELEADFEHMTRFQQGRLLVFKGTSFIYSGQYQEALDNLIQSENLVQN
SDYLFSAYSYEATAYIALRNFKDAFVVMSKSLGLIDRIDNIDIKRAAYLRLASLYSAMGIKEEVGTYAAKAESLASADNV
KDICTAKLYLSVYQLELEAFTKAFDEFQYTHDFCESNGFPLISFIALKGMGESKLRLQEYSAAVALLLKALKGYESFNFQ
LEINSVHSLLTEAYFALKQSAEVVEHAKFVTSLPNDPSYTELKHTVFKVLAGLNAENKQFEQAYEYSKKEQYYNQLMFDE
AKMKTLAYQAAKFNADEQSREINLLNKERELYVAQQTVKEREYTNMLMFVTILVGGLFFLGILLAVGQMQKRKFMRMARM
DALTGILNRGAGQELGENLFVQATARGGDFCVILFDLDFFKRINDSFGHGTGDWALKKVVDSLKSHIRNGDVFARIGGEE
FAILLPYADEEKGMEVAEQCRARIEAIDTQHSGHKYVITASFGVSGLMADDLSLDPLFHRADMALYAAKANGRNAVVRYS
DILKCDKQATDLRRHIASPSV

Sequences:

>Translated_581_residues
MPLMAATINFDEVLADLEQLQHTDMQSAITKLHELEADFEHMTRFQQGRLLVFKGTSFIYSGQYQEALDNLIQSENLVQN
SDYLFSAYSYEATAYIALRNFKDAFVVMSKSLGLIDRIDNIDIKRAAYLRLASLYSAMGIKEEVGTYAAKAESLASADNV
KDICTAKLYLSVYQLELEAFTKAFDEFQYTHDFCESNGFPLISFIALKGMGESKLRLQEYSAAVALLLKALKGYESFNFQ
LEINSVHSLLTEAYFALKQSAEVVEHAKFVTSLPNDPSYTELKHTVFKVLAGLNAENKQFEQAYEYSKKEQYYNQLMFDE
AKMKTLAYQAAKFNADEQSREINLLNKERELYVAQQTVKEREYTNMLMFVTILVGGLFFLGILLAVGQMQKRKFMRMARM
DALTGILNRGAGQELGENLFVQATARGGDFCVILFDLDFFKRINDSFGHGTGDWALKKVVDSLKSHIRNGDVFARIGGEE
FAILLPYADEEKGMEVAEQCRARIEAIDTQHSGHKYVITASFGVSGLMADDLSLDPLFHRADMALYAAKANGRNAVVRYS
DILKCDKQATDLRRHIASPSV
>Mature_580_residues
PLMAATINFDEVLADLEQLQHTDMQSAITKLHELEADFEHMTRFQQGRLLVFKGTSFIYSGQYQEALDNLIQSENLVQNS
DYLFSAYSYEATAYIALRNFKDAFVVMSKSLGLIDRIDNIDIKRAAYLRLASLYSAMGIKEEVGTYAAKAESLASADNVK
DICTAKLYLSVYQLELEAFTKAFDEFQYTHDFCESNGFPLISFIALKGMGESKLRLQEYSAAVALLLKALKGYESFNFQL
EINSVHSLLTEAYFALKQSAEVVEHAKFVTSLPNDPSYTELKHTVFKVLAGLNAENKQFEQAYEYSKKEQYYNQLMFDEA
KMKTLAYQAAKFNADEQSREINLLNKERELYVAQQTVKEREYTNMLMFVTILVGGLFFLGILLAVGQMQKRKFMRMARMD
ALTGILNRGAGQELGENLFVQATARGGDFCVILFDLDFFKRINDSFGHGTGDWALKKVVDSLKSHIRNGDVFARIGGEEF
AILLPYADEEKGMEVAEQCRARIEAIDTQHSGHKYVITASFGVSGLMADDLSLDPLFHRADMALYAAKANGRNAVVRYSD
ILKCDKQATDLRRHIASPSV

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=35.9756097560976, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI87081881, Length=160, Percent_Identity=39.375, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=35.4430379746835, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI87082007, Length=191, Percent_Identity=34.5549738219895, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788381, Length=177, Percent_Identity=31.638418079096, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=33.9393939393939, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=29.0909090909091, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787802, Length=187, Percent_Identity=33.1550802139037, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=33.7423312883436, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI87081974, Length=194, Percent_Identity=28.3505154639175, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87081977, Length=151, Percent_Identity=33.112582781457, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788956, Length=174, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787056, Length=205, Percent_Identity=32.6829268292683, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=28.6624203821656, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 65575; Mature: 65443

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLMAATINFDEVLADLEQLQHTDMQSAITKLHELEADFEHMTRFQQGRLLVFKGTSFIY
CCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCEEE
SGQYQEALDNLIQSENLVQNSDYLFSAYSYEATAYIALRNFKDAFVVMSKSLGLIDRIDN
CCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHCCCHHHHCCC
IDIKRAAYLRLASLYSAMGIKEEVGTYAAKAESLASADNVKDICTAKLYLSVYQLELEAF
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TKAFDEFQYTHDFCESNGFPLISFIALKGMGESKLRLQEYSAAVALLLKALKGYESFNFQ
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LEINSVHSLLTEAYFALKQSAEVVEHAKFVTSLPNDPSYTELKHTVFKVLAGLNAENKQF
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
EQAYEYSKKEQYYNQLMFDEAKMKTLAYQAAKFNADEQSREINLLNKERELYVAQQTVKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHCHHHHHHHHHHHHHH
REYTNMLMFVTILVGGLFFLGILLAVGQMQKRKFMRMARMDALTGILNRGAGQELGENLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEE
VQATARGGDFCVILFDLDFFKRINDSFGHGTGDWALKKVVDSLKSHIRNGDVFARIGGEE
EEEEECCCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
FAILLPYADEEKGMEVAEQCRARIEAIDTQHSGHKYVITASFGVSGLMADDLSLDPLFHR
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCHHHHH
ADMALYAAKANGRNAVVRYSDILKCDKQATDLRRHIASPSV
HHHEEEEECCCCCCEEEEHHHHHHHCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PLMAATINFDEVLADLEQLQHTDMQSAITKLHELEADFEHMTRFQQGRLLVFKGTSFIY
CCEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCEEE
SGQYQEALDNLIQSENLVQNSDYLFSAYSYEATAYIALRNFKDAFVVMSKSLGLIDRIDN
CCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHCCCHHHHCCC
IDIKRAAYLRLASLYSAMGIKEEVGTYAAKAESLASADNVKDICTAKLYLSVYQLELEAF
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TKAFDEFQYTHDFCESNGFPLISFIALKGMGESKLRLQEYSAAVALLLKALKGYESFNFQ
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LEINSVHSLLTEAYFALKQSAEVVEHAKFVTSLPNDPSYTELKHTVFKVLAGLNAENKQF
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
EQAYEYSKKEQYYNQLMFDEAKMKTLAYQAAKFNADEQSREINLLNKERELYVAQQTVKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHCHHHHHHHHHHHHHH
REYTNMLMFVTILVGGLFFLGILLAVGQMQKRKFMRMARMDALTGILNRGAGQELGENLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEE
VQATARGGDFCVILFDLDFFKRINDSFGHGTGDWALKKVVDSLKSHIRNGDVFARIGGEE
EEEEECCCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
FAILLPYADEEKGMEVAEQCRARIEAIDTQHSGHKYVITASFGVSGLMADDLSLDPLFHR
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCHHHHH
ADMALYAAKANGRNAVVRYSDILKCDKQATDLRRHIASPSV
HHHEEEEECCCCCCEEEEHHHHHHHCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]