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The map label for this gene is nemA [H]

Identifier: 160874446

GI number: 160874446

Start: 1576705

End: 1577772

Strand: Direct

Name: nemA [H]

Synonym: Sbal195_1327

Alternate gene names: 160874446

Gene position: 1576705-1577772 (Clockwise)

Preceding gene: 160874444

Following gene: 160874448

Centisome position: 29.49

GC content: 48.69

Gene sequence:

>1068_bases
ATGACCACACAAACCACGCCAAATTCCTTGGCAAATCTGTTCGAAACCTACTCGCTTAATGACACTATTACCTTAAACAA
TCGTTTCTTAATGGCACCTTTGACCCGTTGTATGGCCGATGCCGACTTAGTGCCAACCGGTGATATGGTTGCCTATTATG
CACGCCGCGCCGAAGCGGGTTTGATCATCACAGAGGCGACCATTATTCGTCCCGATGCCCAAGGTTATCCAAATACCCCT
GGTATTTTTACTCAAGCGCAAATTGCTGGCTGGCGCAAAGTGACCGATGCAGTACATGCCAATGGCGGTAAGATTTTTGT
GCAGTTATGGCATACGGGTCGTGTTGCACATCCACACTTTTTTGGTGGTGGCGATGTGCTTGCCGCATCAGCACAGAAGA
TTGAAGGCAGTGTGCCAAGAATGCGTGAGCTAACTTACGTCACGCCAAAACCAGCCACACTCGAGGATATTCAAGGCCTA
GTGCGTGATTACGCCAAAGCGGCGGAAAATGCCATTGAAGCAGGTTTTGATGGCGTTGAAATCCACGGTGCAAATGGTTA
TTTAATTGATACTTTCTTACATCATGACAGCAATCGCCGCACCGATGAATACGGTGGCACGCCAGCCAATATGTCCCGTT
TTGCTCTTGAAGTTGTTGACGCTGTAGTGGCTCGTATCGGCAAAGACAGAACTGGTCTACGGATTTCACCTGGGGCTTAT
TTCAATATGGCGGCCGATAGCCGTGACCGCGAAGTGTTCGATTACTTCTTGCCGGAACTAGAGTCACGCGATATCGCCTT
TGTGCATATTGGGATTTTCGATGACAGTATGGAATTCGACTATCTTGGCGGCAGAGCTTCAAGCTATGTGCGCGCACACT
ATGGCAAAACCTTAGTGGGAGTGGGAAGCTACAGCGCTGAAACTGCCAGTGAAGCGATTGCAGCGAATAAGTTCGATTTG
ATTGCTATCGGTCGTCCATTCATTGCTAACCCTGACTATGTTGCTCGCGTGCGTGATGGCGTTGAACTTGTTCCTTACAG
CGACGAAATGCTAGCAAGCTTAGTGTAA

Upstream 100 bases:

>100_bases
CCGAATTCTACCTAATTTTGAATGAACATTCAAAAATTAACTTGAATGATCGTTCAAATAATAATATCTTGAATGCATAT
TCAAGAATGGGACTGAGAAA

Downstream 100 bases:

>100_bases
TTTCACTTAATAGCTGAATAAAGAAAGGGTTAGCATTGGCTAACCCTTTTTGTTTGTCTTTCTCGCGTTTTTATTTAATC
CGCTTTAACTAAGGATCTAA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTTQTTPNSLANLFETYSLNDTITLNNRFLMAPLTRCMADADLVPTGDMVAYYARRAEAGLIITEATIIRPDAQGYPNTP
GIFTQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHFFGGGDVLAASAQKIEGSVPRMRELTYVTPKPATLEDIQGL
VRDYAKAAENAIEAGFDGVEIHGANGYLIDTFLHHDSNRRTDEYGGTPANMSRFALEVVDAVVARIGKDRTGLRISPGAY
FNMAADSRDREVFDYFLPELESRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVGVGSYSAETASEAIAANKFDL
IAIGRPFIANPDYVARVRDGVELVPYSDEMLASLV

Sequences:

>Translated_355_residues
MTTQTTPNSLANLFETYSLNDTITLNNRFLMAPLTRCMADADLVPTGDMVAYYARRAEAGLIITEATIIRPDAQGYPNTP
GIFTQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHFFGGGDVLAASAQKIEGSVPRMRELTYVTPKPATLEDIQGL
VRDYAKAAENAIEAGFDGVEIHGANGYLIDTFLHHDSNRRTDEYGGTPANMSRFALEVVDAVVARIGKDRTGLRISPGAY
FNMAADSRDREVFDYFLPELESRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVGVGSYSAETASEAIAANKFDL
IAIGRPFIANPDYVARVRDGVELVPYSDEMLASLV
>Mature_354_residues
TTQTTPNSLANLFETYSLNDTITLNNRFLMAPLTRCMADADLVPTGDMVAYYARRAEAGLIITEATIIRPDAQGYPNTPG
IFTQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHFFGGGDVLAASAQKIEGSVPRMRELTYVTPKPATLEDIQGLV
RDYAKAAENAIEAGFDGVEIHGANGYLIDTFLHHDSNRRTDEYGGTPANMSRFALEVVDAVVARIGKDRTGLRISPGAYF
NMAADSRDREVFDYFLPELESRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVGVGSYSAETASEAIAANKFDLI
AIGRPFIANPDYVARVRDGVELVPYSDEMLASLV

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=341, Percent_Identity=41.0557184750733, Blast_Score=240, Evalue=9e-65,
Organism=Escherichia coli, GI1789463, Length=347, Percent_Identity=28.8184438040346, Blast_Score=118, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17559804, Length=359, Percent_Identity=27.2980501392758, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17565138, Length=342, Percent_Identity=28.3625730994152, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI72001454, Length=350, Percent_Identity=27.1428571428571, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=350, Percent_Identity=27.1428571428571, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17559802, Length=365, Percent_Identity=27.3972602739726, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=363, Percent_Identity=25.3443526170799, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325086, Length=364, Percent_Identity=37.3626373626374, Blast_Score=195, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6321973, Length=358, Percent_Identity=37.1508379888268, Blast_Score=188, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38832; Mature: 38701

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQTTPNSLANLFETYSLNDTITLNNRFLMAPLTRCMADADLVPTGDMVAYYARRAEAG
CCCCCCHHHHHHHHHHCCCCCEEEECCEEEHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
LIITEATIIRPDAQGYPNTPGIFTQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHF
EEEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEE
FGGGDVLAASAQKIEGSVPRMRELTYVTPKPATLEDIQGLVRDYAKAAENAIEAGFDGVE
ECCCCEEEHHHHHHCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHGANGYLIDTFLHHDSNRRTDEYGGTPANMSRFALEVVDAVVARIGKDRTGLRISPGAY
EECCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCE
FNMAADSRDREVFDYFLPELESRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVG
EEECCCCCCHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHCCCEEEE
VGSYSAETASEAIAANKFDLIAIGRPFIANPDYVARVRDGVELVPYSDEMLASLV
ECCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCEEECCCHHHHHHCC
>Mature Secondary Structure 
TTQTTPNSLANLFETYSLNDTITLNNRFLMAPLTRCMADADLVPTGDMVAYYARRAEAG
CCCCCHHHHHHHHHHCCCCCEEEECCEEEHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
LIITEATIIRPDAQGYPNTPGIFTQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHF
EEEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEE
FGGGDVLAASAQKIEGSVPRMRELTYVTPKPATLEDIQGLVRDYAKAAENAIEAGFDGVE
ECCCCEEEHHHHHHCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHGANGYLIDTFLHHDSNRRTDEYGGTPANMSRFALEVVDAVVARIGKDRTGLRISPGAY
EECCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCE
FNMAADSRDREVFDYFLPELESRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVG
EEECCCCCCHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHCCCEEEE
VGSYSAETASEAIAANKFDLIAIGRPFIANPDYVARVRDGVELVPYSDEMLASLV
ECCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]