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The map label for this gene is potH [H]

Identifier: 160874320

GI number: 160874320

Start: 1424866

End: 1425771

Strand: Direct

Name: potH [H]

Synonym: Sbal195_1201

Alternate gene names: 160874320

Gene position: 1424866-1425771 (Clockwise)

Preceding gene: 160874319

Following gene: 160874321

Centisome position: 26.65

GC content: 45.03

Gene sequence:

>906_bases
ATGAAGATACCGACTAAATTAAAGTGGCTCAAAGGCCGCATTTGGACCATAGGCATACCCTATGCTTGGCTGTTATTGTT
CTTCGCCTTGCCATTTGCGATTGTCCTTAAACTGAGTTTTTCGACGGCGGCGATTGCGATCCCACCCTATGAGGCCACGT
TCCAATATGCCGATGATGTGTTAAATATCTTCCTGCATTTTGGTAACTATTTGATGTTGCTCGATGATTCGCTGTATTAC
ATGGCGTATCTCAGCTCATTGAAAATGGCGTTAATTTCGACCATCGGCTGTTTGATTATTGGCTATCCAATGGCCTATGC
CATTGCCCGTGCCCCAGCGCGTTTGCAAACTGTATTGCTGCTGTTAGTCATGTTGCCTTCTTGGACCTCATTTTTAATCC
GGGTTTACGCTTGGATGGGGATCTTAAGTAACACAGGGATCATCAACCATTCGCTGATGTGGCTGGGAATCATCTCTGAG
CCGTTACAAATCCTCAATACCAATATTGCCGTGTATATCGGCATCATCTATGCGTATTTACCTTTTATGATTTTGCCCTT
GTACGCCACCTTAGTGAAACTCGATATGAGTTTGATTGAAGCCGCGTCTGACTTAGGCTCAAGCCGTTTAAATACCTTCT
GGAAAATCACTTTTCCGCTGTCAAAAAGCGGTGTGATTGCCGGTTCTATGTTGGTGTTTATTCCTGCCGTGGGTGAGTTT
GTGATCCCTGAGCTACTCGGTGGTCCAGATTCCTTAATGATAGGTCGAGTCTTATGGCAAGAATTCTTCAATAACCGCGA
CTGGCCTGTGGCTTCGTCACTGGCGATTGTAATGCTGGGCTTATTGATTATTCCTATTACCTTATTCCATCGCTATCAGG
CGCGTAGTTTGGAGAAAGACCTATGA

Upstream 100 bases:

>100_bases
AGTGCAGTATGACTAACCGTGAACGCCGCGCCGATCATGCCACATGGGATGACGAAGTGTTCATCAGTTGGGAGGACACC
AGTGGCGTGGTGTTAAGATC

Downstream 100 bases:

>100_bases
AAAAGTTAAGTTTTTCTTCAGCGATGTTGTGGTTTGGCTTGTTCTTCCTGTATGCACCTATGTTAGTGCTGGTGATTTAC
TCCTTTAACGACTCTAAATT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKIPTKLKWLKGRIWTIGIPYAWLLLFFALPFAIVLKLSFSTAAIAIPPYEATFQYADDVLNIFLHFGNYLMLLDDSLYY
MAYLSSLKMALISTIGCLIIGYPMAYAIARAPARLQTVLLLLVMLPSWTSFLIRVYAWMGILSNTGIINHSLMWLGIISE
PLQILNTNIAVYIGIIYAYLPFMILPLYATLVKLDMSLIEAASDLGSSRLNTFWKITFPLSKSGVIAGSMLVFIPAVGEF
VIPELLGGPDSLMIGRVLWQEFFNNRDWPVASSLAIVMLGLLIIPITLFHRYQARSLEKDL

Sequences:

>Translated_301_residues
MKIPTKLKWLKGRIWTIGIPYAWLLLFFALPFAIVLKLSFSTAAIAIPPYEATFQYADDVLNIFLHFGNYLMLLDDSLYY
MAYLSSLKMALISTIGCLIIGYPMAYAIARAPARLQTVLLLLVMLPSWTSFLIRVYAWMGILSNTGIINHSLMWLGIISE
PLQILNTNIAVYIGIIYAYLPFMILPLYATLVKLDMSLIEAASDLGSSRLNTFWKITFPLSKSGVIAGSMLVFIPAVGEF
VIPELLGGPDSLMIGRVLWQEFFNNRDWPVASSLAIVMLGLLIIPITLFHRYQARSLEKDL
>Mature_301_residues
MKIPTKLKWLKGRIWTIGIPYAWLLLFFALPFAIVLKLSFSTAAIAIPPYEATFQYADDVLNIFLHFGNYLMLLDDSLYY
MAYLSSLKMALISTIGCLIIGYPMAYAIARAPARLQTVLLLLVMLPSWTSFLIRVYAWMGILSNTGIINHSLMWLGIISE
PLQILNTNIAVYIGIIYAYLPFMILPLYATLVKLDMSLIEAASDLGSSRLNTFWKITFPLSKSGVIAGSMLVFIPAVGEF
VIPELLGGPDSLMIGRVLWQEFFNNRDWPVASSLAIVMLGLLIIPITLFHRYQARSLEKDL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=296, Percent_Identity=61.4864864864865, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1787369, Length=266, Percent_Identity=41.7293233082707, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1787713, Length=225, Percent_Identity=30.6666666666667, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33830; Mature: 33830

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPTKLKWLKGRIWTIGIPYAWLLLFFALPFAIVLKLSFSTAAIAIPPYEATFQYADDV
CCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
LNIFLHFGNYLMLLDDSLYYMAYLSSLKMALISTIGCLIIGYPMAYAIARAPARLQTVLL
HHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LLVMLPSWTSFLIRVYAWMGILSNTGIINHSLMWLGIISEPLQILNTNIAVYIGIIYAYL
HHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
PFMILPLYATLVKLDMSLIEAASDLGSSRLNTFWKITFPLSKSGVIAGSMLVFIPAVGEF
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
VIPELLGGPDSLMIGRVLWQEFFNNRDWPVASSLAIVMLGLLIIPITLFHRYQARSLEKD
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
L
C
>Mature Secondary Structure
MKIPTKLKWLKGRIWTIGIPYAWLLLFFALPFAIVLKLSFSTAAIAIPPYEATFQYADDV
CCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
LNIFLHFGNYLMLLDDSLYYMAYLSSLKMALISTIGCLIIGYPMAYAIARAPARLQTVLL
HHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LLVMLPSWTSFLIRVYAWMGILSNTGIINHSLMWLGIISEPLQILNTNIAVYIGIIYAYL
HHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
PFMILPLYATLVKLDMSLIEAASDLGSSRLNTFWKITFPLSKSGVIAGSMLVFIPAVGEF
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
VIPELLGGPDSLMIGRVLWQEFFNNRDWPVASSLAIVMLGLLIIPITLFHRYQARSLEKD
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]