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The map label for this gene is ispH [H]

Identifier: 160874279

GI number: 160874279

Start: 1373207

End: 1374163

Strand: Direct

Name: ispH [H]

Synonym: Sbal195_1159

Alternate gene names: 160874279

Gene position: 1373207-1374163 (Clockwise)

Preceding gene: 160874278

Following gene: 160874280

Centisome position: 25.68

GC content: 47.02

Gene sequence:

>957_bases
ATGAAACTCGATCCTACCAGCCCAAGTTTGAATATTATGCTCGCGAACCCGCGTGGTTTTTGTGCGGGGGTTGATCGTGC
TATCAGTATTGTTGAACGGGCGTTGGAATTGTTTTCACCGCCTATCTATGTTCGACATGAAGTCGTACATAATCGCTATG
TCGTGCAGAATTTGAAGGATCGCGGTGCGGTTTTTGTCGAAGAGTTAGATCAAGTGCCGGATAACAACATTGTTATCTTC
AGTGCCCATGGTGTATCTCAAGCGGTGAGACAAGAAGCTAAGGCTCGAGGGTTGAGAGTTTTCGATGCGACTTGTCCCTT
AGTGACCAAAGTGCACCTTCAAGTGACGCGTGCAAGCCGTAAAGGTATTGAATGTATCTTGATTGGCCACGAGGGGCACC
CTGAAGTCGAAGGCACTATGGGTCAGTATGATAATCCTAACGGCGGCGTTTATCTGATTGAATCGCCTGCCGATGTAGAT
GCTCTCGAAGTTCGCGATCCTAACAATCTCTGCTTCGTGACCCAAACAACTTTATCTGTCGATGATACGTTAGACATTAT
TGCCGCGTTGCAAAAAAGGTTTCCTTCAATTGAAGGACCTCGTAAAGACGATATCTGTTATGCAACACAGAATCGTCAAG
ATGCGGTACGTAATCTGTCTGCAGATGTGGATTTGTTGATTGTTGTTGGTTCAAAAAACAGCTCTAACTCTAATCGGTTA
CGCGAGCTCGCATTGAAGACCGGAACCCAGTCTTATCTGGTGGATACTGCTGACGATGTTGAATCAAGCTGGTTCGATAA
TGTTTCGCGTGTTGCTGTTACCGCAGGCGCTTCTGCACCTGAGGTCTTAGTTCAACAAGTTGTCCAAGCGATTGCGAAAC
TCGCTCCTAGTGTGGTGACTGAAGTCGAAGGACGTAAGGAAGACACTGTCTTTGCGGTGCCTGCCGAACTGCGTTAA

Upstream 100 bases:

>100_bases
GTCCGTGATGTCGCGGGTGATTCCATCACAGTCGATCTGAATCATCCTCTGGCAGGGCACAAGATCACTTTTGAACTGGA
CGTTGTAAAGGTACTTTAAG

Downstream 100 bases:

>100_bases
ATCTATGTGATGTTAAGTTATTTATAAAAAAGAGGCGATAAGCCTCTTTTTTATTGCTTCAAATTCATTCAAGGCATAAT
GATGTCAATGCTTATATGCT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKLDPTSPSLNIMLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKDRGAVFVEELDQVPDNNIVIF
SAHGVSQAVRQEAKARGLRVFDATCPLVTKVHLQVTRASRKGIECILIGHEGHPEVEGTMGQYDNPNGGVYLIESPADVD
ALEVRDPNNLCFVTQTTLSVDDTLDIIAALQKRFPSIEGPRKDDICYATQNRQDAVRNLSADVDLLIVVGSKNSSNSNRL
RELALKTGTQSYLVDTADDVESSWFDNVSRVAVTAGASAPEVLVQQVVQAIAKLAPSVVTEVEGRKEDTVFAVPAELR

Sequences:

>Translated_318_residues
MKLDPTSPSLNIMLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKDRGAVFVEELDQVPDNNIVIF
SAHGVSQAVRQEAKARGLRVFDATCPLVTKVHLQVTRASRKGIECILIGHEGHPEVEGTMGQYDNPNGGVYLIESPADVD
ALEVRDPNNLCFVTQTTLSVDDTLDIIAALQKRFPSIEGPRKDDICYATQNRQDAVRNLSADVDLLIVVGSKNSSNSNRL
RELALKTGTQSYLVDTADDVESSWFDNVSRVAVTAGASAPEVLVQQVVQAIAKLAPSVVTEVEGRKEDTVFAVPAELR
>Mature_318_residues
MKLDPTSPSLNIMLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKDRGAVFVEELDQVPDNNIVIF
SAHGVSQAVRQEAKARGLRVFDATCPLVTKVHLQVTRASRKGIECILIGHEGHPEVEGTMGQYDNPNGGVYLIESPADVD
ALEVRDPNNLCFVTQTTLSVDDTLDIIAALQKRFPSIEGPRKDDICYATQNRQDAVRNLSADVDLLIVVGSKNSSNSNRL
RELALKTGTQSYLVDTADDVESSWFDNVSRVAVTAGASAPEVLVQQVVQAIAKLAPSVVTEVEGRKEDTVFAVPAELR

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=65.3721682847896, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34741; Mature: 34741

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDPTSPSLNIMLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKD
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHC
RGAVFVEELDQVPDNNIVIFSAHGVSQAVRQEAKARGLRVFDATCPLVTKVHLQVTRASR
CCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECCC
KGIECILIGHEGHPEVEGTMGQYDNPNGGVYLIESPADVDALEVRDPNNLCFVTQTTLSV
CCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCEEEEEEEEECC
DDTLDIIAALQKRFPSIEGPRKDDICYATQNRQDAVRNLSADVDLLIVVGSKNSSNSNRL
CHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCHHHH
RELALKTGTQSYLVDTADDVESSWFDNVSRVAVTAGASAPEVLVQQVVQAIAKLAPSVVT
HHHHHHCCCCCEEEECHHHHHHHHHCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
EVEGRKEDTVFAVPAELR
HCCCCCCCEEEECCCCCC
>Mature Secondary Structure
MKLDPTSPSLNIMLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKD
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHC
RGAVFVEELDQVPDNNIVIFSAHGVSQAVRQEAKARGLRVFDATCPLVTKVHLQVTRASR
CCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECCC
KGIECILIGHEGHPEVEGTMGQYDNPNGGVYLIESPADVDALEVRDPNNLCFVTQTTLSV
CCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCEEEEEEEEECC
DDTLDIIAALQKRFPSIEGPRKDDICYATQNRQDAVRNLSADVDLLIVVGSKNSSNSNRL
CHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCHHHH
RELALKTGTQSYLVDTADDVESSWFDNVSRVAVTAGASAPEVLVQQVVQAIAKLAPSVVT
HHHHHHCCCCCEEEECHHHHHHHHHCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
EVEGRKEDTVFAVPAELR
HCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]