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The map label for this gene is gshA [H]

Identifier: 160874252

GI number: 160874252

Start: 1341826

End: 1343475

Strand: Direct

Name: gshA [H]

Synonym: Sbal195_1132

Alternate gene names: 160874252

Gene position: 1341826-1343475 (Clockwise)

Preceding gene: 160874251

Following gene: 160874253

Centisome position: 25.09

GC content: 46.91

Gene sequence:

>1650_bases
TTGCAAGATAAGCCTCAAAATAAAGGTCTGACTGTTAACCTTGTCGCGATAGCGAATCACCAATTAGAGAAGCGCAAATT
GAAGTCATTCAATGAATTAGTACAACATCTCTCGGACGCCCATGGGCGCACCGCACTCTTGGGTATGTTACGTGGCATAG
AACGTGAGGCGTTGCGGATTGATGAGTCGGGTTATCTCGCCACCGATTCGCATCCTGAAGCCTTAGGCTCAGCGTTAATG
CATTCGCGTATTACGACGGATTACAGTGAAGCCTTGCTCGAGTTTATTACTCCGGTACATAACAATGTTGAGCAACTGCT
AAACGGATTAACTGAAACCCACGCCTTCAGCGTGCGCCATTTAAATGGTCAGCGCCTGTGGCCTGTGAGCATGCCTTGTT
ATGTGAAGGACGAGAAACAGATCCCCGTTGCCCGTTATGGTTCATCAAATACCGGTATGATGAAAACCTTATATCGTAAG
GGCTTAACCTATCGTTACGGCGCCTTGATGCAGATTATTTCTGGGGTGCACTTTAACTTTTCGGTATCACAAGAGTTATG
GCAAAGCCTGTACGAATTGAGCGATAAGCAGTTAAGTTACGATGATTTTATTTCTGAATCCTATTTTGGCTTGATCCGTA
ACTACCGCCGTTTAGTCTGGGTGCTACCTTATTTATTTGGTGCCTCACCAGCATTATGTAGCTCCTTCATTAAAGATCAA
AAAACCGATCTCAAGTTTGAAAAAACGGGTCACGGTACTCTGTATCTACCCTATGCGACTTCGTTGCGTATGAGCGATTT
AGGCTACACCAACAAAGAGCAAGAAGAGCTGAATATCAGCTATAACTCTTTGCCTGAGTATCTGGCTGGCGTGAATGCGG
CCATCAAAATGCCTTCGGCAAACTTTGCCAATATCGGCGTTAAAGTCGATGGTGAGTATCGTCAGCTCAATGCCAACGTA
TTGCAGATTGAAAACGAGTTCTACTCGCCAATCCGTGCAAAACGGGTGACGCGCAGTGGCGAGAAGCCGTCAGAAGCCTT
AGCCCGTGCAGGGGTGGAATACATTGAAGTGCGTGCTTTAGATGTTAATCCATTTAGCCCGATTGGCATTGAGGCGACCC
AAGTGCGCTTCCTTGATTTATTCCTACTCTATTGTTTATTAACGCAGTCGCCTAAGTCTGAAGCCAGCGAAGAGGCGAGA
TTAAGCGCGAATCTTAAAGCTGTGGTGCTTGAAGGCCGTAAACCTGGGCTTGAGTTACTAACCGCAAACGGTTCACGTTC
GCTTAAATCTTGGTTAGAAGAGTTGTTTGATGCCTTGCAACCTTTGGCGGTATTGCTCGATGGTGAGGCGACGGATTATC
AAGTGGCGTTAGCCCATTGGCGTAAAGCGGTTGAAGATCCTAACTTGACCCTGTCGGGACAAGTGCTGCAAAACGTAATC
AACAAAGGCCAAGACCATGGTCAGTGGGTGATGACGTTGGCACAGCAATACTATCAATTCTTTGTCGATTATCCCTTGTC
GAGCGAGACCATAGCGCAGTATCAAGCCGAAGCATCCAGCTCGCTTAACAAGCAGGCCGAGCTAGAAGCGGCGCAGCGTT
CAGTTTCGTTTGATGATTATCTGGAAGATTACTTTGGAGCTCAGTCGTGA

Upstream 100 bases:

>100_bases
AGACGGTGACAGCATTAGCTGTCACCGTCTGCTTTTAACAGTACAAGTTGTGAATTTGGCTTGCCCCACAGACAGGTTTA
GATTTATTTTGAGGCGTATT

Downstream 100 bases:

>100_bases
AAGAGGGCTTATTCTCTTTCACTCGCTTGCGTATCAGCGCAGCTTTGATTGGGGCCAGTGCCTTATTATCAGGCTGCGCC
GCTTCACCAGAAGATCCACT

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MQDKPQNKGLTVNLVAIANHQLEKRKLKSFNELVQHLSDAHGRTALLGMLRGIEREALRIDESGYLATDSHPEALGSALM
HSRITTDYSEALLEFITPVHNNVEQLLNGLTETHAFSVRHLNGQRLWPVSMPCYVKDEKQIPVARYGSSNTGMMKTLYRK
GLTYRYGALMQIISGVHFNFSVSQELWQSLYELSDKQLSYDDFISESYFGLIRNYRRLVWVLPYLFGASPALCSSFIKDQ
KTDLKFEKTGHGTLYLPYATSLRMSDLGYTNKEQEELNISYNSLPEYLAGVNAAIKMPSANFANIGVKVDGEYRQLNANV
LQIENEFYSPIRAKRVTRSGEKPSEALARAGVEYIEVRALDVNPFSPIGIEATQVRFLDLFLLYCLLTQSPKSEASEEAR
LSANLKAVVLEGRKPGLELLTANGSRSLKSWLEELFDALQPLAVLLDGEATDYQVALAHWRKAVEDPNLTLSGQVLQNVI
NKGQDHGQWVMTLAQQYYQFFVDYPLSSETIAQYQAEASSSLNKQAELEAAQRSVSFDDYLEDYFGAQS

Sequences:

>Translated_549_residues
MQDKPQNKGLTVNLVAIANHQLEKRKLKSFNELVQHLSDAHGRTALLGMLRGIEREALRIDESGYLATDSHPEALGSALM
HSRITTDYSEALLEFITPVHNNVEQLLNGLTETHAFSVRHLNGQRLWPVSMPCYVKDEKQIPVARYGSSNTGMMKTLYRK
GLTYRYGALMQIISGVHFNFSVSQELWQSLYELSDKQLSYDDFISESYFGLIRNYRRLVWVLPYLFGASPALCSSFIKDQ
KTDLKFEKTGHGTLYLPYATSLRMSDLGYTNKEQEELNISYNSLPEYLAGVNAAIKMPSANFANIGVKVDGEYRQLNANV
LQIENEFYSPIRAKRVTRSGEKPSEALARAGVEYIEVRALDVNPFSPIGIEATQVRFLDLFLLYCLLTQSPKSEASEEAR
LSANLKAVVLEGRKPGLELLTANGSRSLKSWLEELFDALQPLAVLLDGEATDYQVALAHWRKAVEDPNLTLSGQVLQNVI
NKGQDHGQWVMTLAQQYYQFFVDYPLSSETIAQYQAEASSSLNKQAELEAAQRSVSFDDYLEDYFGAQS
>Mature_549_residues
MQDKPQNKGLTVNLVAIANHQLEKRKLKSFNELVQHLSDAHGRTALLGMLRGIEREALRIDESGYLATDSHPEALGSALM
HSRITTDYSEALLEFITPVHNNVEQLLNGLTETHAFSVRHLNGQRLWPVSMPCYVKDEKQIPVARYGSSNTGMMKTLYRK
GLTYRYGALMQIISGVHFNFSVSQELWQSLYELSDKQLSYDDFISESYFGLIRNYRRLVWVLPYLFGASPALCSSFIKDQ
KTDLKFEKTGHGTLYLPYATSLRMSDLGYTNKEQEELNISYNSLPEYLAGVNAAIKMPSANFANIGVKVDGEYRQLNANV
LQIENEFYSPIRAKRVTRSGEKPSEALARAGVEYIEVRALDVNPFSPIGIEATQVRFLDLFLLYCLLTQSPKSEASEEAR
LSANLKAVVLEGRKPGLELLTANGSRSLKSWLEELFDALQPLAVLLDGEATDYQVALAHWRKAVEDPNLTLSGQVLQNVI
NKGQDHGQWVMTLAQQYYQFFVDYPLSSETIAQYQAEASSSLNKQAELEAAQRSVSFDDYLEDYFGAQS

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=494, Percent_Identity=50.6072874493927, Blast_Score=485, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 61827; Mature: 61827

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDKPQNKGLTVNLVAIANHQLEKRKLKSFNELVQHLSDAHGRTALLGMLRGIEREALRI
CCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHEE
DESGYLATDSHPEALGSALMHSRITTDYSEALLEFITPVHNNVEQLLNGLTETHAFSVRH
CCCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEE
LNGQRLWPVSMPCYVKDEKQIPVARYGSSNTGMMKTLYRKGLTYRYGALMQIISGVHFNF
CCCCEEEECCCCEEECCCCCCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCE
SVSQELWQSLYELSDKQLSYDDFISESYFGLIRNYRRLVWVLPYLFGASPALCSSFIKDQ
EHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
KTDLKFEKTGHGTLYLPYATSLRMSDLGYTNKEQEELNISYNSLPEYLAGVNAAIKMPSA
CCCCEEEECCCCEEEECCCCCEEHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCEEECCCC
NFANIGVKVDGEYRQLNANVLQIENEFYSPIRAKRVTRSGEKPSEALARAGVEYIEVRAL
CCEEEEEEECCCHHHHCCCEEEECHHHHCHHHHHHHHCCCCCHHHHHHHCCCEEEEEEEE
DVNPFSPIGIEATQVRFLDLFLLYCLLTQSPKSEASEEARLSANLKAVVLEGRKPGLELL
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEE
TANGSRSLKSWLEELFDALQPLAVLLDGEATDYQVALAHWRKAVEDPNLTLSGQVLQNVI
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHH
NKGQDHGQWVMTLAQQYYQFFVDYPLSSETIAQYQAEASSSLNKQAELEAAQRSVSFDDY
HCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHH
LEDYFGAQS
HHHHCCCCC
>Mature Secondary Structure
MQDKPQNKGLTVNLVAIANHQLEKRKLKSFNELVQHLSDAHGRTALLGMLRGIEREALRI
CCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHEE
DESGYLATDSHPEALGSALMHSRITTDYSEALLEFITPVHNNVEQLLNGLTETHAFSVRH
CCCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEE
LNGQRLWPVSMPCYVKDEKQIPVARYGSSNTGMMKTLYRKGLTYRYGALMQIISGVHFNF
CCCCEEEECCCCEEECCCCCCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCE
SVSQELWQSLYELSDKQLSYDDFISESYFGLIRNYRRLVWVLPYLFGASPALCSSFIKDQ
EHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
KTDLKFEKTGHGTLYLPYATSLRMSDLGYTNKEQEELNISYNSLPEYLAGVNAAIKMPSA
CCCCEEEECCCCEEEECCCCCEEHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCEEECCCC
NFANIGVKVDGEYRQLNANVLQIENEFYSPIRAKRVTRSGEKPSEALARAGVEYIEVRAL
CCEEEEEEECCCHHHHCCCEEEECHHHHCHHHHHHHHCCCCCHHHHHHHCCCEEEEEEEE
DVNPFSPIGIEATQVRFLDLFLLYCLLTQSPKSEASEEARLSANLKAVVLEGRKPGLELL
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEE
TANGSRSLKSWLEELFDALQPLAVLLDGEATDYQVALAHWRKAVEDPNLTLSGQVLQNVI
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHH
NKGQDHGQWVMTLAQQYYQFFVDYPLSSETIAQYQAEASSSLNKQAELEAAQRSVSFDDY
HCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHH
LEDYFGAQS
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]