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The map label for this gene is hprA [H]

Identifier: 160874207

GI number: 160874207

Start: 1285717

End: 1286667

Strand: Direct

Name: hprA [H]

Synonym: Sbal195_1087

Alternate gene names: 160874207

Gene position: 1285717-1286667 (Clockwise)

Preceding gene: 160874206

Following gene: 160874208

Centisome position: 24.04

GC content: 49.74

Gene sequence:

>951_bases
ATGAAGATAGTTGTACTCGATGGCGAAACCTTAAATCCTGGCGATCTCAGTTGGCAATCCTTGACTGAATTAGGCGAGTT
TGCGTGTTTCCCTCGCACCGCTGGCGCCGATATTGTTGCGCGTGCCCAAAGTGCAGAGATCATTCTCACCAATAAAACGC
CGCTCAATGCTAAGACTCTGGCGCAACTTCCGGCACTGAAATACATAGGCGTATTGGCAACGGGTACTAACGTTGTCGAT
ATAGCGGCTGCAAAAGCGCGCGGAATAGTGGTAACGAATGTGCCCGCCTATGCGCCCGATGCCGTAGCGCAGATGGTTTT
TGCCCATATACTGCATCACACCCAAGCCGTGGCTGCCCATCATCAAGCCGTGGTCGATGGTCTGTGGGTGAACTGCCAAG
ATTTTTGTTTCACCTTAATGCCGCTGCAATCCTTAAAAGGCAAGACTTTAGGTTTAATAGGTTATGGTGATATCGGCCAG
CAGGTTGGGACGCTCGCCCTTGCATTCGGGATGAAAGTGCTCGTCAATACCCGCTCTGTTATCAAAGACTTGCCCCAAGG
TTCCACTTGGGTACCGCGTGATACTGTGCTGAGCGAATCAGATATTATCTCGCTGCATTGCCCGCTTACCCCTGAGACTG
AGCAATTTATCAATCACCAAGCACTCGCCTTGATGAAGCCTAATGCGCTGCTTATTAATACGGCGCGCGGTGGATTAATT
GATGAGTCCGCGCTTGCAGCAGCGCTTTCACAAAACCACTTATTTGCCGGTGTCGATGTATTATCGACCGAGCCGCCCAG
CGCGGATAATCCCTTATTGCATGCACCAAATATCAGCATTAGTCCCCATAATGCGTGGGCGACTAAGGAAGCCAGACAAA
GTCTACTCAATATTGCCGTTGCGAATGTGCACGGTTATTTACAGGGTGAAGTGATTAATTGTGTGAATTAA

Upstream 100 bases:

>100_bases
GGCACATTACGTCGGATGTGATGTGGCAGGCGCAGGCTAACCTTGGGCTCAAAGTCAGTTTTTAGCGCTAACACATCGTC
TTAAAAGGAGATTTAGCGGC

Downstream 100 bases:

>100_bases
GGAATGCATTAGAACAAGGAATGTTATGTATAAGATAATTTTGATTATAGTTTTTGGATTAGTGTTTTGTGGGTGTGCTA
GCAAGAGTCGAGTTCCTAAA

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKIVVLDGETLNPGDLSWQSLTELGEFACFPRTAGADIVARAQSAEIILTNKTPLNAKTLAQLPALKYIGVLATGTNVVD
IAAAKARGIVVTNVPAYAPDAVAQMVFAHILHHTQAVAAHHQAVVDGLWVNCQDFCFTLMPLQSLKGKTLGLIGYGDIGQ
QVGTLALAFGMKVLVNTRSVIKDLPQGSTWVPRDTVLSESDIISLHCPLTPETEQFINHQALALMKPNALLINTARGGLI
DESALAAALSQNHLFAGVDVLSTEPPSADNPLLHAPNISISPHNAWATKEARQSLLNIAVANVHGYLQGEVINCVN

Sequences:

>Translated_316_residues
MKIVVLDGETLNPGDLSWQSLTELGEFACFPRTAGADIVARAQSAEIILTNKTPLNAKTLAQLPALKYIGVLATGTNVVD
IAAAKARGIVVTNVPAYAPDAVAQMVFAHILHHTQAVAAHHQAVVDGLWVNCQDFCFTLMPLQSLKGKTLGLIGYGDIGQ
QVGTLALAFGMKVLVNTRSVIKDLPQGSTWVPRDTVLSESDIISLHCPLTPETEQFINHQALALMKPNALLINTARGGLI
DESALAAALSQNHLFAGVDVLSTEPPSADNPLLHAPNISISPHNAWATKEARQSLLNIAVANVHGYLQGEVINCVN
>Mature_316_residues
MKIVVLDGETLNPGDLSWQSLTELGEFACFPRTAGADIVARAQSAEIILTNKTPLNAKTLAQLPALKYIGVLATGTNVVD
IAAAKARGIVVTNVPAYAPDAVAQMVFAHILHHTQAVAAHHQAVVDGLWVNCQDFCFTLMPLQSLKGKTLGLIGYGDIGQ
QVGTLALAFGMKVLVNTRSVIKDLPQGSTWVPRDTVLSESDIISLHCPLTPETEQFINHQALALMKPNALLINTARGGLI
DESALAAALSQNHLFAGVDVLSTEPPSADNPLLHAPNISISPHNAWATKEARQSLLNIAVANVHGYLQGEVINCVN

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=273, Percent_Identity=34.4322344322344, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI4557499, Length=273, Percent_Identity=34.4322344322344, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI145580575, Length=273, Percent_Identity=34.4322344322344, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI61743967, Length=273, Percent_Identity=33.3333333333333, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4557497, Length=273, Percent_Identity=33.3333333333333, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI23308577, Length=260, Percent_Identity=30.7692307692308, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI6912396, Length=251, Percent_Identity=28.6852589641434, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI87082289, Length=280, Percent_Identity=34.2857142857143, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789279, Length=278, Percent_Identity=34.1726618705036, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787645, Length=263, Percent_Identity=33.8403041825095, Blast_Score=121, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17532191, Length=278, Percent_Identity=29.136690647482, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25147481, Length=273, Percent_Identity=29.6703296703297, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320925, Length=282, Percent_Identity=32.6241134751773, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6322116, Length=284, Percent_Identity=32.3943661971831, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324055, Length=324, Percent_Identity=26.2345679012346, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324964, Length=252, Percent_Identity=27.3809523809524, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6321253, Length=182, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571528, Length=288, Percent_Identity=33.6805555555556, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45552429, Length=263, Percent_Identity=32.6996197718631, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI28574286, Length=270, Percent_Identity=31.4814814814815, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI28574284, Length=263, Percent_Identity=32.6996197718631, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24585514, Length=263, Percent_Identity=32.6996197718631, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28574282, Length=263, Percent_Identity=32.6996197718631, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45551003, Length=263, Percent_Identity=32.6996197718631, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646446, Length=273, Percent_Identity=31.8681318681319, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24646448, Length=273, Percent_Identity=31.8681318681319, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24646452, Length=273, Percent_Identity=31.8681318681319, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24646450, Length=273, Percent_Identity=31.8681318681319, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI62472511, Length=273, Percent_Identity=31.8681318681319, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24585516, Length=261, Percent_Identity=28.735632183908, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19921140, Length=253, Percent_Identity=28.4584980237154, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 33545; Mature: 33545

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVLDGETLNPGDLSWQSLTELGEFACFPRTAGADIVARAQSAEIILTNKTPLNAKTL
CEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHEEECCCCEEEEECCCCCCHHHH
AQLPALKYIGVLATGTNVVDIAAAKARGIVVTNVPAYAPDAVAQMVFAHILHHTQAVAAH
HHCCHHHHEEEEECCCCEEEEEHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
HQAVVDGLWVNCQDFCFTLMPLQSLKGKTLGLIGYGDIGQQVGTLALAFGMKVLVNTRSV
HHHHHHCEECCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHH
IKDLPQGSTWVPRDTVLSESDIISLHCPLTPETEQFINHQALALMKPNALLINTARGGLI
HHHCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHHCCCEEEEECCCEEEEECCCCCCC
DESALAAALSQNHLFAGVDVLSTEPPSADNPLLHAPNISISPHNAWATKEARQSLLNIAV
CHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEECCCCEECCCCCHHHHHHHHHHHHHHH
ANVHGYLQGEVINCVN
HHHCCEEECCEEECCC
>Mature Secondary Structure
MKIVVLDGETLNPGDLSWQSLTELGEFACFPRTAGADIVARAQSAEIILTNKTPLNAKTL
CEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHEEECCCCEEEEECCCCCCHHHH
AQLPALKYIGVLATGTNVVDIAAAKARGIVVTNVPAYAPDAVAQMVFAHILHHTQAVAAH
HHCCHHHHEEEEECCCCEEEEEHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
HQAVVDGLWVNCQDFCFTLMPLQSLKGKTLGLIGYGDIGQQVGTLALAFGMKVLVNTRSV
HHHHHHCEECCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHH
IKDLPQGSTWVPRDTVLSESDIISLHCPLTPETEQFINHQALALMKPNALLINTARGGLI
HHHCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHHCCCEEEEECCCEEEEECCCCCCC
DESALAAALSQNHLFAGVDVLSTEPPSADNPLLHAPNISISPHNAWATKEARQSLLNIAV
CHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEECCCCEECCCCCHHHHHHHHHHHHHHH
ANVHGYLQGEVINCVN
HHHCCEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]