Click here to switch to the map view.

The map label for this gene is cobQ [H]

Identifier: 160874134

GI number: 160874134

Start: 1208951

End: 1210648

Strand: Direct

Name: cobQ [H]

Synonym: Sbal195_1014

Alternate gene names: 160874134

Gene position: 1208951-1210648 (Clockwise)

Preceding gene: 160874133

Following gene: 160874135

Centisome position: 22.61

GC content: 51.12

Gene sequence:

>1698_bases
ATGCCCTCTCCAAACACAGACAACACAGGCTGTGCAACTGCACTCGATACCGCTAACGCTTTAGCTATTCATTCTGCTGC
TAATCTTGTGGTGAATACGCCAGCGGTTAATTCTGCGGTGAATACGCCTGCGGTTAATTCGCTTCGTCGTGCAAAAGTCT
TGATGGTGCAGGGCACAACGTCGGATGCGGGCAAGAGCACCTTAGTGGCAGGCATTTGTCGTTTGCTCGCCCGAGAGGGC
GTAAAGGTTGCGCCCTTTAAGCCGCAAAACATGGCGCTCAATAGCGCAGTGACTATTGATGGCGGCGAGATTGGCCGCGC
ACAGGCGTTGCAAGCCGTGGCCTGTTACCTTGAGCCCCATACCGATTTTAATCCTATTCTGCTCAAACCTAGCTCGGACA
CGGGCGCGCAAATTATCGTTCACGGCAAAGCCTTGACGACGCTGGAAGCCTCGGCCTTTTTTGGGCTTAAATCTAAGGAC
TACAAGGCAATGGCGCTCGGCGCTGTGCTGGAATCCTTCGAGCGATTAAGTGGCCAGTATCAAACCATAGTGGTGGAAGG
CGCGGGGAGTCCGGCGGAGATCAATCTGCGCGAGGGCGATATTGCCAACATGGGATTTGCCGAAGCCGTAGATTGCCCTG
TGATCATCATTGCCGATATCGATAAGGGCGGCGTATTTGCCCATTTGGTCGGGACGCTGGCGCTGTTATCTGAGTCTGAA
CAGGCGCGGGTAAAGGGCTTTGTGATCAATCGTTTCCGTGGCGATATTAGCTTGTTGCAATCTGGCCTCGATTGGCTTGA
AGCCTATACTCAAAAGCCTGTTTTAGGTGTGTTGCCTTATCTGCATGATTTACATCTCGATGCCGAAGATGCGCTGATAA
GCTCGCCGACTAAAGGCGCTAACAGCCGTTTAAAAGTGCGGGTATTAGTGTACCCAAGGATCAGTAATCATACGGATTTT
GATCCGCTGCGGTTGCATCCCGATATCGATTTTGACTATGTGTCATTGCAAACCCAAGCCGCGAGTCACGCCACTCATTT
ACCCGCTGCCGATTTATTGATCTTGCCCGGCAGTAAGAATGTGCGCGCCGATTTGGCCTTTTTACGCCAGCAAGGTTGGG
ATAAGGATATCGCCACTCACCTGCGTTACGGCGGCAAAGTGCTTGGTATTTGCGGTGGTTACCAAATGTTAGGTCAAAGC
ATTGCCGATCCCTTAGGGATTGAGGACAGTGCGGGCGATACCGCAGGACTTGGCTACTTACCATTGAGCACAGAACTGAA
AGCCGATAAACAGTTACGGCGGGTGAGCGGTGAGCTAACGTTACTTGGACAAAAGGTTGCTGTGCAGGGCTATGAAATCC
ACTGCGGCGAATCACGTTATCTTATCTCTAGGCTTGAACAAACGAATCCGCCCTTGCAGTTAATCCCAGAATCGACATCA
TTAACCGCGCCAGCGATGAGTTTTGCCGATGGCCTGCAAAGTGATGATGGGCAAATCTTAGGTACTTATTTACACGGGCT
GTTTGATAGCCCTGATGCCTGTCAACTGATCCTGCGCTGGGCGGGATTAAACGATGCTAAGGCGATAGATATCAATCAAA
TACGTGAGCAGCAACTTGAGCGTTTAGCCGATGTACTAGCCGAGCATCTAGATATGCAAAAATTACAGACTATTTGGGAT
GCCGCAGTTCGGCGTTGA

Upstream 100 bases:

>100_bases
TACCCTTGGCACTCAAACCACTTTAATCTAACTAAGTTAAGTGATGTTGAGACTGAGCTTTTATCTTTATCCCTGCAAAC
TAGAATAAGTTTTTAACCCT

Downstream 100 bases:

>100_bases
ATTCGGCAAAGAATGGATATTGTGAGAGCGGCATTTAGAAACTAACCATTTGCCGCTGTCTATTGAACCATTTATAAAGC
AGACATTTTTAGCAGCAGAG

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MPSPNTDNTGCATALDTANALAIHSAANLVVNTPAVNSAVNTPAVNSLRRAKVLMVQGTTSDAGKSTLVAGICRLLAREG
VKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVACYLEPHTDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGLKSKD
YKAMALGAVLESFERLSGQYQTIVVEGAGSPAEINLREGDIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSESE
QARVKGFVINRFRGDISLLQSGLDWLEAYTQKPVLGVLPYLHDLHLDAEDALISSPTKGANSRLKVRVLVYPRISNHTDF
DPLRLHPDIDFDYVSLQTQAASHATHLPAADLLILPGSKNVRADLAFLRQQGWDKDIATHLRYGGKVLGICGGYQMLGQS
IADPLGIEDSAGDTAGLGYLPLSTELKADKQLRRVSGELTLLGQKVAVQGYEIHCGESRYLISRLEQTNPPLQLIPESTS
LTAPAMSFADGLQSDDGQILGTYLHGLFDSPDACQLILRWAGLNDAKAIDINQIREQQLERLADVLAEHLDMQKLQTIWD
AAVRR

Sequences:

>Translated_565_residues
MPSPNTDNTGCATALDTANALAIHSAANLVVNTPAVNSAVNTPAVNSLRRAKVLMVQGTTSDAGKSTLVAGICRLLAREG
VKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVACYLEPHTDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGLKSKD
YKAMALGAVLESFERLSGQYQTIVVEGAGSPAEINLREGDIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSESE
QARVKGFVINRFRGDISLLQSGLDWLEAYTQKPVLGVLPYLHDLHLDAEDALISSPTKGANSRLKVRVLVYPRISNHTDF
DPLRLHPDIDFDYVSLQTQAASHATHLPAADLLILPGSKNVRADLAFLRQQGWDKDIATHLRYGGKVLGICGGYQMLGQS
IADPLGIEDSAGDTAGLGYLPLSTELKADKQLRRVSGELTLLGQKVAVQGYEIHCGESRYLISRLEQTNPPLQLIPESTS
LTAPAMSFADGLQSDDGQILGTYLHGLFDSPDACQLILRWAGLNDAKAIDINQIREQQLERLADVLAEHLDMQKLQTIWD
AAVRR
>Mature_564_residues
PSPNTDNTGCATALDTANALAIHSAANLVVNTPAVNSAVNTPAVNSLRRAKVLMVQGTTSDAGKSTLVAGICRLLAREGV
KVAPFKPQNMALNSAVTIDGGEIGRAQALQAVACYLEPHTDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGLKSKDY
KAMALGAVLESFERLSGQYQTIVVEGAGSPAEINLREGDIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSESEQ
ARVKGFVINRFRGDISLLQSGLDWLEAYTQKPVLGVLPYLHDLHLDAEDALISSPTKGANSRLKVRVLVYPRISNHTDFD
PLRLHPDIDFDYVSLQTQAASHATHLPAADLLILPGSKNVRADLAFLRQQGWDKDIATHLRYGGKVLGICGGYQMLGQSI
ADPLGIEDSAGDTAGLGYLPLSTELKADKQLRRVSGELTLLGQKVAVQGYEIHCGESRYLISRLEQTNPPLQLIPESTSL
TAPAMSFADGLQSDDGQILGTYLHGLFDSPDACQLILRWAGLNDAKAIDINQIREQQLERLADVLAEHLDMQKLQTIWDA
AVRR

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 60618; Mature: 60487

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPNTDNTGCATALDTANALAIHSAANLVVNTPAVNSAVNTPAVNSLRRAKVLMVQGTT
CCCCCCCCCCCHHHHCCCCEEEEECCCCEEEECCCCCCCCCCCHHHHHCCEEEEEEECCC
SDAGKSTLVAGICRLLAREGVKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVACYLEPH
CCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEECCEEEECCCCCCHHHHHHHHHHEECCC
TDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGLKSKDYKAMALGAVLESFERLSGQY
CCCCCEEEECCCCCCCEEEEECCEEEEEEHHHEECCCCCCCHHHHHHHHHHHHHHHCCCE
QTIVVEGAGSPAEINLREGDIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSESE
EEEEEECCCCCCEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
QARVKGFVINRFRGDISLLQSGLDWLEAYTQKPVLGVLPYLHDLHLDAEDALISSPTKGA
HHHHHEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCCC
NSRLKVRVLVYPRISNHTDFDPLRLHPDIDFDYVSLQTQAASHATHLPAADLLILPGSKN
CCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEHHHHHHHCCCCCCCEEEECCCCC
VRADLAFLRQQGWDKDIATHLRYGGKVLGICGGYQMLGQSIADPLGIEDSAGDTAGLGYL
HHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCEEE
PLSTELKADKQLRRVSGELTLLGQKVAVQGYEIHCGESRYLISRLEQTNPPLQLIPESTS
CCCCCHHHHHHHHHHCCCEEEEECEEEEECEEEEECCHHHHHHHHHHCCCCEEEECCCCC
LTAPAMSFADGLQSDDGQILGTYLHGLFDSPDACQLILRWAGLNDAKAIDINQIREQQLE
CCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECHHHHHHHHHH
RLADVLAEHLDMQKLQTIWDAAVRR
HHHHHHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSPNTDNTGCATALDTANALAIHSAANLVVNTPAVNSAVNTPAVNSLRRAKVLMVQGTT
CCCCCCCCCCHHHHCCCCEEEEECCCCEEEECCCCCCCCCCCHHHHHCCEEEEEEECCC
SDAGKSTLVAGICRLLAREGVKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVACYLEPH
CCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEECCEEEECCCCCCHHHHHHHHHHEECCC
TDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGLKSKDYKAMALGAVLESFERLSGQY
CCCCCEEEECCCCCCCEEEEECCEEEEEEHHHEECCCCCCCHHHHHHHHHHHHHHHCCCE
QTIVVEGAGSPAEINLREGDIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSESE
EEEEEECCCCCCEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
QARVKGFVINRFRGDISLLQSGLDWLEAYTQKPVLGVLPYLHDLHLDAEDALISSPTKGA
HHHHHEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCCC
NSRLKVRVLVYPRISNHTDFDPLRLHPDIDFDYVSLQTQAASHATHLPAADLLILPGSKN
CCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEHHHHHHHCCCCCCCEEEECCCCC
VRADLAFLRQQGWDKDIATHLRYGGKVLGICGGYQMLGQSIADPLGIEDSAGDTAGLGYL
HHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCEEE
PLSTELKADKQLRRVSGELTLLGQKVAVQGYEIHCGESRYLISRLEQTNPPLQLIPESTS
CCCCCHHHHHHHHHHCCCEEEEECEEEEECEEEEECCHHHHHHHHHHCCCCEEEECCCCC
LTAPAMSFADGLQSDDGQILGTYLHGLFDSPDACQLILRWAGLNDAKAIDINQIREQQLE
CCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECHHHHHHHHHH
RLADVLAEHLDMQKLQTIWDAAVRR
HHHHHHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]