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The map label for this gene is cobS [H]

Identifier: 160874132

GI number: 160874132

Start: 1207471

End: 1208259

Strand: Direct

Name: cobS [H]

Synonym: Sbal195_1012

Alternate gene names: 160874132

Gene position: 1207471-1208259 (Clockwise)

Preceding gene: 160874131

Following gene: 160874133

Centisome position: 22.58

GC content: 50.7

Gene sequence:

>789_bases
ATGTCAGAACGCGAAAGCTGGCACAAAGAGATAGATTTGTTTTTAGTTGCCATGGGCTATTTTACCCGCATTCCTATGCC
TAAATGGGTCGAAGTCGATAGCGATAAGTTGAATAAGGCCAGCCGTTATTTCGGTCTCGTCGGTCTTTTGGTCGGCCTGT
TAAGTGCCATTGTATTTTGGTTGACGCAGAACTGGCTCCCCGCCGGCGTCTCAGTATTGTTAGCTATGTTGGTTGGCGTC
TTGCTCACGGGCGGATTTCACGAGGATGGCCTAGCCGATACCTTCGACGGTTTTGGCGGTGGTTGGACGGCGGAAGACAA
GCTACGGATCATGAAGGACTCGCGCCTTGGCAGTTATGGCGCACTGGCGTTGATGTTGGCCCTGCTGCTGAAATGGCAGT
TGTTAGTCGAGTTGGCGCTGTACGATCCTGTGGTCGCGGGCTCGGCGCTTATCGTCGCGCACACAGTCAGCCGCGTGGTA
TCAGCCAGTATCATCTTTAGTGAAAAATATGTCCGCGATGACGAGACCAGTAAGTCTAAGCCGCTATCGCAACATCAAGG
CATTAATGAGCTGCTTATCCTGATCGCCAGCGGCGTATTAGTGTTGTTATTCCTCAAGGGGTTAGCGGCATTGTCATTGC
TGTTAGTGATGATAGGACTGCGCCGTTTGATCATAGTGATCTTTAGACGCCAGATTGGCGGTTACACGGGTGATACCTTA
GGCGCGGCGCAGCAAATAGCTGAAATCGTGTGTTATTTTGTATTGCTCGTTGTAGGTAACATACTGTGA

Upstream 100 bases:

>100_bases
CTGCTAAGTCGCTTATTTTACTTATTTAAAATAATAACTTTTTAGCCAACCGTTTTAATCTTAAACCTTGCAGCAAGTGT
TAATTCACTAAGGATGCTTT

Downstream 100 bases:

>100_bases
TCCACCTCGTCCTAGGCGGCGCCCGTAGCGGTAAGAGTCGCTACGGCGAAGCCTTAGTGCGCCAGTACACTGCGCTCGGC
TTCGATGCCTGCTATGTGGC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSERESWHKEIDLFLVAMGYFTRIPMPKWVEVDSDKLNKASRYFGLVGLLVGLLSAIVFWLTQNWLPAGVSVLLAMLVGV
LLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYGALALMLALLLKWQLLVELALYDPVVAGSALIVAHTVSRVV
SASIIFSEKYVRDDETSKSKPLSQHQGINELLILIASGVLVLLFLKGLAALSLLLVMIGLRRLIIVIFRRQIGGYTGDTL
GAAQQIAEIVCYFVLLVVGNIL

Sequences:

>Translated_262_residues
MSERESWHKEIDLFLVAMGYFTRIPMPKWVEVDSDKLNKASRYFGLVGLLVGLLSAIVFWLTQNWLPAGVSVLLAMLVGV
LLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYGALALMLALLLKWQLLVELALYDPVVAGSALIVAHTVSRVV
SASIIFSEKYVRDDETSKSKPLSQHQGINELLILIASGVLVLLFLKGLAALSLLLVMIGLRRLIIVIFRRQIGGYTGDTL
GAAQQIAEIVCYFVLLVVGNIL
>Mature_261_residues
SERESWHKEIDLFLVAMGYFTRIPMPKWVEVDSDKLNKASRYFGLVGLLVGLLSAIVFWLTQNWLPAGVSVLLAMLVGVL
LTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYGALALMLALLLKWQLLVELALYDPVVAGSALIVAHTVSRVVS
ASIIFSEKYVRDDETSKSKPLSQHQGINELLILIASGVLVLLFLKGLAALSLLLVMIGLRRLIIVIFRRQIGGYTGDTLG
AAQQIAEIVCYFVLLVVGNIL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=35.2226720647773, Blast_Score=131, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 28677; Mature: 28546

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERESWHKEIDLFLVAMGYFTRIPMPKWVEVDSDKLNKASRYFGLVGLLVGLLSAIVFW
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTQNWLPAGVSVLLAMLVGVLLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYG
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCHH
ALALMLALLLKWQLLVELALYDPVVAGSALIVAHTVSRVVSASIIFSEKYVRDDETSKSK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PLSQHQGINELLILIASGVLVLLFLKGLAALSLLLVMIGLRRLIIVIFRRQIGGYTGDTL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
GAAQQIAEIVCYFVLLVVGNIL
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SERESWHKEIDLFLVAMGYFTRIPMPKWVEVDSDKLNKASRYFGLVGLLVGLLSAIVFW
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTQNWLPAGVSVLLAMLVGVLLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYG
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCHH
ALALMLALLLKWQLLVELALYDPVVAGSALIVAHTVSRVVSASIIFSEKYVRDDETSKSK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PLSQHQGINELLILIASGVLVLLFLKGLAALSLLLVMIGLRRLIIVIFRRQIGGYTGDTL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
GAAQQIAEIVCYFVLLVVGNIL
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA