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The map label for this gene is cysJ [H]

Identifier: 160874073

GI number: 160874073

Start: 1128318

End: 1130117

Strand: Direct

Name: cysJ [H]

Synonym: Sbal195_0953

Alternate gene names: 160874073

Gene position: 1128318-1130117 (Clockwise)

Preceding gene: 160874070

Following gene: 160874074

Centisome position: 21.1

GC content: 49.22

Gene sequence:

>1800_bases
ATGTTGTTAAAAGAACTTTCATCGCTGGCTTCGCCATTGTCTCAAGGACAAGTCGAGAAGCTAAAACAACTCACTTCTGA
GTTAAGTGCTGTGCAACTGGCTTGGGTGAGTGGCTATCTTGCTGCCACCGCCAATGCTGGCCAACTAGCTCCCGCCACCG
AAGCGCAAACTGCTCAAACAGTGACGATTCTTTATGGCAGTCAAACGGGTAATGGCCGTGGTGTCGCCAAAGCGCTCGCC
GATAAAGCACAGGCGCAAGGTTATGCGGTCAATCTGGCTTCAATGGGCGAATACAATGTTCGCCAACTGAAACAAGAAAC
TGTGCTCTTGCTGGTTGTCAGCACCCATGGTGAAGGTGAAGCGCCGGATGACGCGATTGAATTACATAAGTTTTTAGCAT
CGAAACGTGCGCCTAAGTTAGACAATCTACATTACTCAGTACTTGCGCTGGGGGATTCAAGTTATGAATTCTTCTGCCAA
ACCGGTAAAGACTTTAGTACTCGTTTAGCGCTACTGGGTGCTAAGTCATTGTTACCTTTGGTTGAATGTGATGTGGACTA
TGAAGCTGCCGCCGGTCAGTGGCACGCCGATGTATTAGAGGCCGTTAAGCCGCTTATCGAAACCTCGTCCGCCAGTGTGG
TATCTATCGGTACCGCTAAAACGATTGGCGAAAGCGAGTTTACTAAGCAAAACCCCTATAGCGCTGAAGTGCTAGCGAGC
CAAAAAATTACCGGCCGCGGTTCAGACCGAGATGTGCGCCACGTTGAAATTGACCTTGGCGACTCAGGCTTAAGCTATCA
AGCGGGCGATGCCCTCGGTGTGTGGTTTAGCAATAATGAAGTGTTAGTCGATGAAGTCCTCGTGGGTCTGTCGTTGGCCG
CCGATGAGTTAGTGACCCTAGGCACAGAATCTTTGACCCTTAAGCAAGCTTTGCTTGAGAAGAAAGAGCTGACTCAGTTG
TATCCTGGATTAGTCAAAGCGTGGGCCGAACTGAGCGGCAGCGCTAAACTGCTGGCATTGAGCGAAGATAAAGAACAAGT
GCGTCACTTTATCCTTAAGCATCAATTTGCGGATTTAGTCGCCCAGTATCCGTTAACAAATAGCGCTGTCACGTTAAGCG
CGGCAAGATTGCTTGAGTTACTGCGCCCATTAACCCCAAGACTTTACTCTATCGCCTCAAGCCAAAGTGAAGTGGAAACC
GAAGTCCATTTAACCGTGGCGTTAGTGGAAGATGAGCGTCACGGCGCGGCCCGTTTTGGCGGAGCGTCACACTTCTTAGC
CTCTGCGCAGGAAGGCACTCAGGTTAAGGTTTATGTTGAGTCGAATAAGCATTTCCGTTTACCTGATAATCCAGACACGC
CAGTGATTATGGTTGGCCCAGGCACAGGTGTTGCGCCATTCCGTGCCTTTATGCAGGAGCGCGTGGCCCAAGGTATTCAA
GGTGATAGCTGGTTATTCTTCGGCAATCCACACTTTGAGCAGGATTTCTTGTATCAAACTGAGTGGCAACAGTATTTGAA
AAATGGCGATTTAAGCCGTATCGACGTGGCGTTCTCGCGCGATCAAGCCCATAAAGTTTATGTGCAGCACCGTATTAAAG
AACAAGGCCAAGCCCTGTGGCAATGGTTGCAAAACGGCGCGCATCTGTACATTTGTGGTGATGCTGAACGTATGGCCAAA
GATGTTCACCAAGCCTTGATTGAGGTGGCTGTCGAAGTCGGTGGTTTGAATAACGAAGCGGCTGAAGCCTACTTTGAAAC
ACTGCGCAGCGATAAACGTTACCAAAAAGACGTGTATTAA

Upstream 100 bases:

>100_bases
TGTATATTCCAAAATGGAATTAAAAATCCGATTTTATTCTTTTTTGCTTTTCTTAAACCGCGATAAGCTTGTGGACATTA
CACTGTCATAGGGCCGTCTC

Downstream 100 bases:

>100_bases
TCTCAACCGTTGCGCATATAAGAAACGCATAAAAATAGCCAAAGCTGCACACGTGATGACCTGTGCAGTCAGCGAACCGA
ATTTGAAGAGGCAGTTGCCA

Product: sulfite reductase (NADPH) flavoprotein subunit alpha

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPATEAQTAQTVTILYGSQTGNGRGVAKALA
DKAQAQGYAVNLASMGEYNVRQLKQETVLLLVVSTHGEGEAPDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQ
TGKDFSTRLALLGAKSLLPLVECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKTIGESEFTKQNPYSAEVLAS
QKITGRGSDRDVRHVEIDLGDSGLSYQAGDALGVWFSNNEVLVDEVLVGLSLAADELVTLGTESLTLKQALLEKKELTQL
YPGLVKAWAELSGSAKLLALSEDKEQVRHFILKHQFADLVAQYPLTNSAVTLSAARLLELLRPLTPRLYSIASSQSEVET
EVHLTVALVEDERHGAARFGGASHFLASAQEGTQVKVYVESNKHFRLPDNPDTPVIMVGPGTGVAPFRAFMQERVAQGIQ
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKVYVQHRIKEQGQALWQWLQNGAHLYICGDAERMAK
DVHQALIEVAVEVGGLNNEAAEAYFETLRSDKRYQKDVY

Sequences:

>Translated_599_residues
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPATEAQTAQTVTILYGSQTGNGRGVAKALA
DKAQAQGYAVNLASMGEYNVRQLKQETVLLLVVSTHGEGEAPDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQ
TGKDFSTRLALLGAKSLLPLVECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKTIGESEFTKQNPYSAEVLAS
QKITGRGSDRDVRHVEIDLGDSGLSYQAGDALGVWFSNNEVLVDEVLVGLSLAADELVTLGTESLTLKQALLEKKELTQL
YPGLVKAWAELSGSAKLLALSEDKEQVRHFILKHQFADLVAQYPLTNSAVTLSAARLLELLRPLTPRLYSIASSQSEVET
EVHLTVALVEDERHGAARFGGASHFLASAQEGTQVKVYVESNKHFRLPDNPDTPVIMVGPGTGVAPFRAFMQERVAQGIQ
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKVYVQHRIKEQGQALWQWLQNGAHLYICGDAERMAK
DVHQALIEVAVEVGGLNNEAAEAYFETLRSDKRYQKDVY
>Mature_599_residues
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPATEAQTAQTVTILYGSQTGNGRGVAKALA
DKAQAQGYAVNLASMGEYNVRQLKQETVLLLVVSTHGEGEAPDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQ
TGKDFSTRLALLGAKSLLPLVECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKTIGESEFTKQNPYSAEVLAS
QKITGRGSDRDVRHVEIDLGDSGLSYQAGDALGVWFSNNEVLVDEVLVGLSLAADELVTLGTESLTLKQALLEKKELTQL
YPGLVKAWAELSGSAKLLALSEDKEQVRHFILKHQFADLVAQYPLTNSAVTLSAARLLELLRPLTPRLYSIASSQSEVET
EVHLTVALVEDERHGAARFGGASHFLASAQEGTQVKVYVESNKHFRLPDNPDTPVIMVGPGTGVAPFRAFMQERVAQGIQ
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKVYVQHRIKEQGQALWQWLQNGAHLYICGDAERMAK
DVHQALIEVAVEVGGLNNEAAEAYFETLRSDKRYQKDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI7657393, Length=592, Percent_Identity=31.5878378378378, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI221316709, Length=592, Percent_Identity=31.5878378378378, Blast_Score=250, Evalue=3e-66,
Organism=Homo sapiens, GI221316705, Length=603, Percent_Identity=31.1774461028192, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI127139033, Length=617, Percent_Identity=30.9562398703404, Blast_Score=245, Evalue=9e-65,
Organism=Homo sapiens, GI221316707, Length=522, Percent_Identity=31.992337164751, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI24041029, Length=598, Percent_Identity=29.4314381270903, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI10835173, Length=646, Percent_Identity=27.7089783281734, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI40254422, Length=647, Percent_Identity=27.047913446677, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI169790956, Length=410, Percent_Identity=30.7317073170732, Blast_Score=159, Evalue=9e-39,
Organism=Homo sapiens, GI169790958, Length=410, Percent_Identity=30.7317073170732, Blast_Score=159, Evalue=1e-38,
Organism=Escherichia coli, GI1789123, Length=593, Percent_Identity=51.0961214165261, Blast_Score=600, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI17554134, Length=596, Percent_Identity=29.8657718120805, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17566446, Length=579, Percent_Identity=28.3246977547496, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17531441, Length=415, Percent_Identity=24.8192771084337, Blast_Score=95, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321832, Length=648, Percent_Identity=30.5555555555556, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6321143, Length=392, Percent_Identity=31.1224489795918, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6325305, Length=635, Percent_Identity=23.1496062992126, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322597, Length=160, Percent_Identity=30.625, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17137192, Length=615, Percent_Identity=29.1056910569106, Blast_Score=224, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24582192, Length=524, Percent_Identity=29.3893129770992, Blast_Score=191, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24660907, Length=596, Percent_Identity=27.6845637583893, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24660903, Length=596, Percent_Identity=27.6845637583893, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI78706872, Length=535, Percent_Identity=27.8504672897196, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24583543, Length=535, Percent_Identity=27.8504672897196, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI78706876, Length=535, Percent_Identity=27.8504672897196, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24647438, Length=236, Percent_Identity=27.1186440677966, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 65451; Mature: 65451

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPATEAQTAQT
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
VTILYGSQTGNGRGVAKALADKAQAQGYAVNLASMGEYNVRQLKQETVLLLVVSTHGEGE
EEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHCCCEEEEEEEECCCCCC
APDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQTGKDFSTRLALLGAKSLLPL
CCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCHH
VECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKTIGESEFTKQNPYSAEVLAS
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHC
QKITGRGSDRDVRHVEIDLGDSGLSYQAGDALGVWFSNNEVLVDEVLVGLSLAADELVTL
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHC
GTESLTLKQALLEKKELTQLYPGLVKAWAELSGSAKLLALSEDKEQVRHFILKHQFADLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
AQYPLTNSAVTLSAARLLELLRPLTPRLYSIASSQSEVETEVHLTVALVEDERHGAARFG
HHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCEEEEEEEEEEECCCCCCHHCC
GASHFLASAQEGTQVKVYVESNKHFRLPDNPDTPVIMVGPGTGVAPFRAFMQERVAQGIQ
CHHHHHHCCCCCCEEEEEEECCCEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCC
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKVYVQHRIKEQGQALW
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
QWLQNGAHLYICGDAERMAKDVHQALIEVAVEVGGLNNEAAEAYFETLRSDKRYQKDVY
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MLLKELSSLASPLSQGQVEKLKQLTSELSAVQLAWVSGYLAATANAGQLAPATEAQTAQT
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
VTILYGSQTGNGRGVAKALADKAQAQGYAVNLASMGEYNVRQLKQETVLLLVVSTHGEGE
EEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHCCCEEEEEEEECCCCCC
APDDAIELHKFLASKRAPKLDNLHYSVLALGDSSYEFFCQTGKDFSTRLALLGAKSLLPL
CCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCHH
VECDVDYEAAAGQWHADVLEAVKPLIETSSASVVSIGTAKTIGESEFTKQNPYSAEVLAS
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHC
QKITGRGSDRDVRHVEIDLGDSGLSYQAGDALGVWFSNNEVLVDEVLVGLSLAADELVTL
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHC
GTESLTLKQALLEKKELTQLYPGLVKAWAELSGSAKLLALSEDKEQVRHFILKHQFADLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
AQYPLTNSAVTLSAARLLELLRPLTPRLYSIASSQSEVETEVHLTVALVEDERHGAARFG
HHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCEEEEEEEEEEECCCCCCHHCC
GASHFLASAQEGTQVKVYVESNKHFRLPDNPDTPVIMVGPGTGVAPFRAFMQERVAQGIQ
CHHHHHHCCCCCCEEEEEEECCCEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCC
GDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLSRIDVAFSRDQAHKVYVQHRIKEQGQALW
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
QWLQNGAHLYICGDAERMAKDVHQALIEVAVEVGGLNNEAAEAYFETLRSDKRYQKDVY
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]