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The map label for this gene is rlmB [H]

Identifier: 160873860

GI number: 160873860

Start: 887840

End: 888580

Strand: Direct

Name: rlmB [H]

Synonym: Sbal195_0738

Alternate gene names: 160873860

Gene position: 887840-888580 (Clockwise)

Preceding gene: 160873859

Following gene: 160873863

Centisome position: 16.6

GC content: 49.8

Gene sequence:

>741_bases
ATGAAAAAACAAGATATTATTTTTGGGATCCACGCAGTTGAAGCCCTGTTAAAACACAGCCCTGAGCGCATTATCGAGTT
ATGGCTGTTACAAGGTCGTGACGATGAACGCTTAACTGTGCTGGCCAATCAGGCCAAAGCCTTTGGCACGACGATTCAAG
TCGCTTCTCGCAAAGCCTTGGATGACAAAGCGGAAAGCAGCCAACATCAAGGTATAGTCGCGCGCGTTAAAGCGGCGAAA
GTGTTGAACGAGCAAGATTTAGATGTGCTGCTTGCTAACACTGAGCAACCATTTCTGCTGATTTTGGATGGCGTGACGGA
TCCCCATAACTTAGGCGCTTGCCTACGTAATGCCGATGCGGCAGGCGTGCAAGGTATTATCGTGCCTAAAGATAACTCAG
TCGGCTTAACTGCGACTGTGAGTAAAGTGGCATGTGGCGCGGCGGAAACTGTGCCTTTATTCCAAGTGACTAATCTTGCG
CGCACTATGCGTCACCTGCAGGATAAAGGTGTATGGATTGTGGGTACGGCGGGCGAGGCAGATTGCGAGCTTTATCAAGC
CGATCTTAAAGGGCCACTGGCGATTGCCATGGGTGCGGAAGATAAAGGTTTACGCCGTTTAAGCCGTGAGTCTTGCGATT
CACTGATCTCTATCCCTATGGCCGGTAGTGTGTCTAGCTTGAACGTATCGGTTGCGACCGGTATTTGCTTATTTGAAGCT
GTGCGTCAGCGTCGTAGCTAA

Upstream 100 bases:

>100_bases
CAAACCTAAGGCTAAAGCGGGCACTAAAGCGAAAAAGACAGCAGTAAAAGATCCGGCTAAAAAGCCAAAAGCCGCTAAGC
GGAGCACGAGAAAGAAATAA

Downstream 100 bases:

>100_bases
TATGCTCATGCTTGCCTGATATCGCACATGCGGTCAGGCTTGCAGTAACGACTTGTTGCAGTCACTATTTGTTGTAGTTA
GCACTTGCAGTAATAAAAAG

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGRDDERLTVLANQAKAFGTTIQVASRKALDDKAESSQHQGIVARVKAAK
VLNEQDLDVLLANTEQPFLLILDGVTDPHNLGACLRNADAAGVQGIIVPKDNSVGLTATVSKVACGAAETVPLFQVTNLA
RTMRHLQDKGVWIVGTAGEADCELYQADLKGPLAIAMGAEDKGLRRLSRESCDSLISIPMAGSVSSLNVSVATGICLFEA
VRQRRS

Sequences:

>Translated_246_residues
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGRDDERLTVLANQAKAFGTTIQVASRKALDDKAESSQHQGIVARVKAAK
VLNEQDLDVLLANTEQPFLLILDGVTDPHNLGACLRNADAAGVQGIIVPKDNSVGLTATVSKVACGAAETVPLFQVTNLA
RTMRHLQDKGVWIVGTAGEADCELYQADLKGPLAIAMGAEDKGLRRLSRESCDSLISIPMAGSVSSLNVSVATGICLFEA
VRQRRS
>Mature_246_residues
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGRDDERLTVLANQAKAFGTTIQVASRKALDDKAESSQHQGIVARVKAAK
VLNEQDLDVLLANTEQPFLLILDGVTDPHNLGACLRNADAAGVQGIIVPKDNSVGLTATVSKVACGAAETVPLFQVTNLA
RTMRHLQDKGVWIVGTAGEADCELYQADLKGPLAIAMGAEDKGLRRLSRESCDSLISIPMAGSVSSLNVSVATGICLFEA
VRQRRS

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=263, Percent_Identity=28.1368821292776, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1790623, Length=240, Percent_Identity=65.4166666666667, Blast_Score=328, Evalue=2e-91,
Organism=Escherichia coli, GI1788935, Length=243, Percent_Identity=27.5720164609054, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790083, Length=158, Percent_Identity=31.6455696202532, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17555908, Length=258, Percent_Identity=27.1317829457364, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=33.9393939393939, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26387; Mature: 26387

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGRDDERLTVLANQAKAFGTTIQVASRKAL
CCCCCEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHCHHHHHHHHHHH
DDKAESSQHQGIVARVKAAKVLNEQDLDVLLANTEQPFLLILDGVTDPHNLGACLRNADA
CCHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCC
AGVQGIIVPKDNSVGLTATVSKVACGAAETVPLFQVTNLARTMRHLQDKGVWIVGTAGEA
CCCEEEEEECCCCCCEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCC
DCELYQADLKGPLAIAMGAEDKGLRRLSRESCDSLISIPMAGSVSSLNVSVATGICLFEA
CHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHEECCCCCCCCCCEEHHHHHHHHHHH
VRQRRS
HHHHCC
>Mature Secondary Structure
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGRDDERLTVLANQAKAFGTTIQVASRKAL
CCCCCEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHCHHHHHHHHHHH
DDKAESSQHQGIVARVKAAKVLNEQDLDVLLANTEQPFLLILDGVTDPHNLGACLRNADA
CCHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCC
AGVQGIIVPKDNSVGLTATVSKVACGAAETVPLFQVTNLARTMRHLQDKGVWIVGTAGEA
CCCEEEEEECCCCCCEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCC
DCELYQADLKGPLAIAMGAEDKGLRRLSRESCDSLISIPMAGSVSSLNVSVATGICLFEA
CHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHEECCCCCCCCCCEEHHHHHHHHHHH
VRQRRS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]