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The map label for this gene is mlaE [H]

Identifier: 160873845

GI number: 160873845

Start: 873756

End: 874541

Strand: Direct

Name: mlaE [H]

Synonym: Sbal195_0723

Alternate gene names: 160873845

Gene position: 873756-874541 (Clockwise)

Preceding gene: 160873844

Following gene: 160873846

Centisome position: 16.34

GC content: 50.0

Gene sequence:

>786_bases
GTGAAGTTAATAGACAGTATTGCCGACTTGGGCCGTAGCGCGATTGAGTTAGTACTCGGTTTTGGCCGCGCAGGACTCAT
GTTGTGGGGGGCGATTTTTCGCTGGCCACGCGTGCGCAAAGGTTTTCCACTATTAGTTCGCCAGATTTATGTGGTCGGCG
TGCAATCTATGGTGATTATTTTAGTTTCAGGGCTTTTTATCGGCATGGTGTTGGCGCTGCAAGGCTACAACATCTTAGTT
GGCTTTGGCACCGAAGAAAGCTTAGGCCCTATGGTGGCGCTCAGTTTACTGCGTGAGTTAGGGCCAGTGGTTACCGCACT
TTTGTTTGCGGGACGTGCGGGTTCAGCCTTAACCGCTGAAATTGGTTTAATGAAAAGTACTGAGCAGCTATCGAGTTTAG
AGATGATGGCTATCGACCCATTAAGGCAGATTATTGCACCACGCTTTTGGGCTGGGGTGATCAGTATGCCATTGCTGGCG
TTAATGTTTACCGCTGTGGGTATTTACGGTGGTCACATAGTAGGCGTGGAATGGAAGGGAATCGACAGCGGGGCGTTTTG
GTCGATTCTGCAGGCGTCGGTCGAATGGCGTCAGGACATAGTTAACTGCTTGATCAAGAGTGGTGTGTTTGCCGTAGTGG
TGACTTGGATTGCGTTATATCGCGGTTATCAAGTGATCCCAAATCCGGAAGGCATCAGCCGTGCAACGACTCAGACTGTG
GTTCAGTCGAGCCTCGCTGTATTGGCATTAGATTTTTTACTGACAGCCCTGATGTTTGGCCGCTAA

Upstream 100 bases:

>100_bases
AACTTAAACAGTTTATTCAAGGCGCGCCGGATGGCCCTGTACCGTTCCATTATCCGGCGAATGATTACCAACAAGAATTA
ATGACAAAAGGAGTTGGCAA

Downstream 100 bases:

>100_bases
CGGAACACTGCGGATTTCGACACAGGTAAGATGGATTAACAGAGTGGTAGCTGAACATGTTGACACGAAAAATTGAATTA
TTAGTGGGTGTGTTTCTTTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKLIDSIADLGRSAIELVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVIILVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIYGGHIVGVEWKGIDSGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVIPNPEGISRATTQTV
VQSSLAVLALDFLLTALMFGR

Sequences:

>Translated_261_residues
MKLIDSIADLGRSAIELVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVIILVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIYGGHIVGVEWKGIDSGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVIPNPEGISRATTQTV
VQSSLAVLALDFLLTALMFGR
>Mature_261_residues
MKLIDSIADLGRSAIELVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVIILVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIYGGHIVGVEWKGIDSGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVIPNPEGISRATTQTV
VQSSLAVLALDFLLTALMFGR

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=259, Percent_Identity=68.7258687258687, Blast_Score=355, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28242; Mature: 28242

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIDSIADLGRSAIELVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVII
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LVSGLFIGMVLALQGYNILVGFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLALMFTAVGIYGGHIVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
IDSGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVIPNPEGISRATTQTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHH
VQSSLAVLALDFLLTALMFGR
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLIDSIADLGRSAIELVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVII
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LVSGLFIGMVLALQGYNILVGFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLALMFTAVGIYGGHIVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
IDSGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVIPNPEGISRATTQTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHH
VQSSLAVLALDFLLTALMFGR
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]