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The map label for this gene is mdeA [H]

Identifier: 160873441

GI number: 160873441

Start: 361680

End: 362840

Strand: Direct

Name: mdeA [H]

Synonym: Sbal195_0316

Alternate gene names: 160873441

Gene position: 361680-362840 (Clockwise)

Preceding gene: 160873440

Following gene: 160873444

Centisome position: 6.76

GC content: 47.8

Gene sequence:

>1161_bases
ATGCAGTTTAAAACCAAATTACTTCACACCCATCATACCGCTGAAAACTCAACGGGTGCGCTCACAACACCTATCTATCA
AAGTAGCACTTTTGCCTATGGCAGTGCCGAAGATGGTCGAGCGCGTTTTGCCGGTGAACAGCCGGGTTTTATCTACAGTC
GTATGGCGAACCCCACAGTACAGGCACTCGAACACCAATTGGCCCAGCTAGAGGGGGCTGATGACGCGCTCGTTGTTGCG
AGTGGTATGGCGGCAATCAGCAGTATTTTCTATGCCTTGGCCAGTCAAGGTGATGAAATCGCATTTATCGATCCTGTATA
TGGTGGTACTGCGGCATTTCTAATCCAAACACTGACCCGCGCGGGCATTACAGTGCGCCGTTATCAAGATGACGACCACT
TATTGTCTGAGATAAGCCCCGAGACAAAAATTATCTTATTTGAGCCTATTACCAATCCGACCTTAAAAGTCAGTGATTAT
CGTAAGGTCAAGCTCGCCGCTGAGCGGGTTGGGGCAATCACCATTTGTGATAATACGTTTTTAACGCCGTATTTATTCAA
ACCGCTGGAACATGGCATAGATATCGTGATGCACAGTGCCACTAAATATTTAAGTGGGCATGGTGATTTAATCGCGGGTG
TGGTCGCGGGCCGTAAAGCATTAATGCAGCCAATTCGCACCATAGCACTTAAGCATATTGGGGCTGCTATTGGGCCACAG
GAAGCCTATTTACTGCAGCGCGGTTTACGTACTTTAGCGTTGCGAATGGATGCCCATCTTGCGGGAGCCGCTAAAGTCGC
TGCTTTTTTAGCATCACATCCGAACGTCGCAAAAGTCTATTATCCAGGGCTTTCACAGGACCCAGGGCATCAAGTTTTGA
GTGAAACGATAAATGCCTTTGGCGGTATGGTGAGTATTGAGCTTGAAGGCGGGTTTAGCCGAGCGGCGCAGTTTTTAGAT
AATTTAAACCTATTTACTCAAGCGGTCAGTTTAGGCGATCTAGAAAGCTTAGCTTGCCACCCAGCAAGTACCACGCATGC
GGCAATGGATGTCGCGAGCAGGCTAGCGTCTGGGGTGAATGATAACTTGATCAGACTGAGTATTGGCGTTGAAGATCCTC
AAGATTTAATCGCCGATCTTGAACAAGCATTAGCGATATAA

Upstream 100 bases:

>100_bases
CCAAAACGTATTAAAAGGGCGTTTACGCTCACATCCTGATGTTGCGAAAGCGCATTACGCCTCATTTGCCAATGCTATTT
AATTGTTATTAGGATCTATT

Downstream 100 bases:

>100_bases
GACGGATAATTTGAATCCGGCCTGTAGCTAAGATCGTGTGTACAACTCGCGGTAGATTTTTCCTGCGGGATAATTTGATG
AATGGTCAATCACTTAGTGT

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MQFKTKLLHTHHTAENSTGALTTPIYQSSTFAYGSAEDGRARFAGEQPGFIYSRMANPTVQALEHQLAQLEGADDALVVA
SGMAAISSIFYALASQGDEIAFIDPVYGGTAAFLIQTLTRAGITVRRYQDDDHLLSEISPETKIILFEPITNPTLKVSDY
RKVKLAAERVGAITICDNTFLTPYLFKPLEHGIDIVMHSATKYLSGHGDLIAGVVAGRKALMQPIRTIALKHIGAAIGPQ
EAYLLQRGLRTLALRMDAHLAGAAKVAAFLASHPNVAKVYYPGLSQDPGHQVLSETINAFGGMVSIELEGGFSRAAQFLD
NLNLFTQAVSLGDLESLACHPASTTHAAMDVASRLASGVNDNLIRLSIGVEDPQDLIADLEQALAI

Sequences:

>Translated_386_residues
MQFKTKLLHTHHTAENSTGALTTPIYQSSTFAYGSAEDGRARFAGEQPGFIYSRMANPTVQALEHQLAQLEGADDALVVA
SGMAAISSIFYALASQGDEIAFIDPVYGGTAAFLIQTLTRAGITVRRYQDDDHLLSEISPETKIILFEPITNPTLKVSDY
RKVKLAAERVGAITICDNTFLTPYLFKPLEHGIDIVMHSATKYLSGHGDLIAGVVAGRKALMQPIRTIALKHIGAAIGPQ
EAYLLQRGLRTLALRMDAHLAGAAKVAAFLASHPNVAKVYYPGLSQDPGHQVLSETINAFGGMVSIELEGGFSRAAQFLD
NLNLFTQAVSLGDLESLACHPASTTHAAMDVASRLASGVNDNLIRLSIGVEDPQDLIADLEQALAI
>Mature_386_residues
MQFKTKLLHTHHTAENSTGALTTPIYQSSTFAYGSAEDGRARFAGEQPGFIYSRMANPTVQALEHQLAQLEGADDALVVA
SGMAAISSIFYALASQGDEIAFIDPVYGGTAAFLIQTLTRAGITVRRYQDDDHLLSEISPETKIILFEPITNPTLKVSDY
RKVKLAAERVGAITICDNTFLTPYLFKPLEHGIDIVMHSATKYLSGHGDLIAGVVAGRKALMQPIRTIALKHIGAAIGPQ
EAYLLQRGLRTLALRMDAHLAGAAKVAAFLASHPNVAKVYYPGLSQDPGHQVLSETINAFGGMVSIELEGGFSRAAQFLD
NLNLFTQAVSLGDLESLACHPASTTHAAMDVASRLASGVNDNLIRLSIGVEDPQDLIADLEQALAI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=388, Percent_Identity=40.7216494845361, Blast_Score=241, Evalue=8e-64,
Organism=Homo sapiens, GI299473759, Length=387, Percent_Identity=37.984496124031, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI25453487, Length=385, Percent_Identity=36.6233766233766, Blast_Score=196, Evalue=4e-50,
Organism=Escherichia coli, GI1790375, Length=369, Percent_Identity=37.3983739837398, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789383, Length=394, Percent_Identity=30.7106598984772, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71980708, Length=398, Percent_Identity=35.1758793969849, Blast_Score=241, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI17560056, Length=397, Percent_Identity=37.7833753148615, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17538055, Length=392, Percent_Identity=37.5, Blast_Score=222, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6319307, Length=395, Percent_Identity=38.9873417721519, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=31.4814814814815, Blast_Score=190, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6321170, Length=335, Percent_Identity=29.2537313432836, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6321254, Length=390, Percent_Identity=28.2051282051282, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321904, Length=401, Percent_Identity=29.6758104738155, Blast_Score=126, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19922554, Length=390, Percent_Identity=37.1794871794872, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 41340; Mature: 41340

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFKTKLLHTHHTAENSTGALTTPIYQSSTFAYGSAEDGRARFAGEQPGFIYSRMANPTV
CCCHHHHHHHHCCCCCCCCCEECCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHCCHHH
QALEHQLAQLEGADDALVVASGMAAISSIFYALASQGDEIAFIDPVYGGTAAFLIQTLTR
HHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHH
AGITVRRYQDDDHLLSEISPETKIILFEPITNPTLKVSDYRKVKLAAERVGAITICDNTF
CCCEEEEECCHHHHHHHCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHCCEEEEECCCC
LTPYLFKPLEHGIDIVMHSATKYLSGHGDLIAGVVAGRKALMQPIRTIALKHIGAAIGPQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EAYLLQRGLRTLALRMDAHLAGAAKVAAFLASHPNVAKVYYPGLSQDPGHQVLSETINAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHH
GGMVSIELEGGFSRAAQFLDNLNLFTQAVSLGDLESLACHPASTTHAAMDVASRLASGVN
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHCCCC
DNLIRLSIGVEDPQDLIADLEQALAI
CCEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQFKTKLLHTHHTAENSTGALTTPIYQSSTFAYGSAEDGRARFAGEQPGFIYSRMANPTV
CCCHHHHHHHHCCCCCCCCCEECCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHCCHHH
QALEHQLAQLEGADDALVVASGMAAISSIFYALASQGDEIAFIDPVYGGTAAFLIQTLTR
HHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHH
AGITVRRYQDDDHLLSEISPETKIILFEPITNPTLKVSDYRKVKLAAERVGAITICDNTF
CCCEEEEECCHHHHHHHCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHCCEEEEECCCC
LTPYLFKPLEHGIDIVMHSATKYLSGHGDLIAGVVAGRKALMQPIRTIALKHIGAAIGPQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EAYLLQRGLRTLALRMDAHLAGAAKVAAFLASHPNVAKVYYPGLSQDPGHQVLSETINAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHH
GGMVSIELEGGFSRAAQFLDNLNLFTQAVSLGDLESLACHPASTTHAAMDVASRLASGVN
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHCCCC
DNLIRLSIGVEDPQDLIADLEQALAI
CCEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]