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The map label for this gene is moaE [H]

Identifier: 160873414

GI number: 160873414

Start: 329395

End: 329862

Strand: Direct

Name: moaE [H]

Synonym: Sbal195_0288

Alternate gene names: 160873414

Gene position: 329395-329862 (Clockwise)

Preceding gene: 160873413

Following gene: 160873415

Centisome position: 6.16

GC content: 50.21

Gene sequence:

>468_bases
ATGCGCTTATTACCCGCAGTTCGAGTGCAAGAAGTCGATTTTAGTGTGGCCGACGAATATCGGCAGTTAGCTCAAGATGA
TAGCGATGGTGCCGTAGTCACTTTCGTCGGTAAAGTACGTGATTTTAACGATGGCGCCGCCGTGACTGACTTAACCTTAG
AGCACTATCCGGGCATGACAGAAGCCGTGCTAGAGCAAATCGTTGTCGAGGCGCGCAGTCGCTGGCCACTGAACAAGGTC
ACTGTGATTCACCGCGTGGGCACTATGGCCTTAGGTGAGCAAATTGTATTTATCGGCGTAACCAGCGCTCATCGCAAAGG
TGCTTTTGCTGCCTGTGAATTTTTAATCGACTTTTTAAAAACCAAAGCGCCATTCTGGAAATTAGAAGCGGGCGAGCAAG
GTAAAAGCTGGGTTGAGGCGAAAGATGCCGATGAACAAGCGGCCGAACTGTGGCAACACAGAGACTAG

Upstream 100 bases:

>100_bases
AAATTGCTGGTAGCGGTAAATCAGACCATCAGTCAATGGGATACGCCTGTAAACGATGGTGATGAAGTCGCCTTTTTCCC
ACCCGTTACCGGAGGTTAAT

Downstream 100 bases:

>100_bases
TTGCTGCAAAAATACGCGCAGTGGCAAGGCAGCTTGCTAGCTCAAGATGAACGCGACCACGGCACTGAGGTGACCCAATG
CAGAGTTTGACCCGTTTCTT

Product: molybdopterin biosynthesis MoaE protein

Products: NA

Alternate protein names: MPT synthase subunit 2; Molybdenum cofactor biosynthesis protein E; Molybdopterin-converting factor large subunit; Molybdopterin-converting factor subunit 2 [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MRLLPAVRVQEVDFSVADEYRQLAQDDSDGAVVTFVGKVRDFNDGAAVTDLTLEHYPGMTEAVLEQIVVEARSRWPLNKV
TVIHRVGTMALGEQIVFIGVTSAHRKGAFAACEFLIDFLKTKAPFWKLEAGEQGKSWVEAKDADEQAAELWQHRD

Sequences:

>Translated_155_residues
MRLLPAVRVQEVDFSVADEYRQLAQDDSDGAVVTFVGKVRDFNDGAAVTDLTLEHYPGMTEAVLEQIVVEARSRWPLNKV
TVIHRVGTMALGEQIVFIGVTSAHRKGAFAACEFLIDFLKTKAPFWKLEAGEQGKSWVEAKDADEQAAELWQHRD
>Mature_155_residues
MRLLPAVRVQEVDFSVADEYRQLAQDDSDGAVVTFVGKVRDFNDGAAVTDLTLEHYPGMTEAVLEQIVVEARSRWPLNKV
TVIHRVGTMALGEQIVFIGVTSAHRKGAFAACEFLIDFLKTKAPFWKLEAGEQGKSWVEAKDADEQAAELWQHRD

Specific function: Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by moaD [H]

COG id: COG0314

COG function: function code H; Molybdopterin converting factor, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaE family [H]

Homologues:

Organism=Homo sapiens, GI4758732, Length=120, Percent_Identity=36.6666666666667, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1787003, Length=138, Percent_Identity=62.3188405797101, Blast_Score=184, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17509279, Length=125, Percent_Identity=36.8, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356259, Length=125, Percent_Identity=36.8, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003448 [H]

Pfam domain/function: PF02391 MoaE [H]

EC number: NA

Molecular weight: Translated: 17318; Mature: 17318

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLPAVRVQEVDFSVADEYRQLAQDDSDGAVVTFVGKVRDFNDGAAVTDLTLEHYPGMT
CCCCCCCCHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCEECCCCCEEEEEEHHHCCCHH
EAVLEQIVVEARSRWPLNKVTVIHRVGTMALGEQIVFIGVTSAHRKGAFAACEFLIDFLK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHH
TKAPFWKLEAGEQGKSWVEAKDADEQAAELWQHRD
CCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MRLLPAVRVQEVDFSVADEYRQLAQDDSDGAVVTFVGKVRDFNDGAAVTDLTLEHYPGMT
CCCCCCCCHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCEECCCCCEEEEEEHHHCCCHH
EAVLEQIVVEARSRWPLNKVTVIHRVGTMALGEQIVFIGVTSAHRKGAFAACEFLIDFLK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHH
TKAPFWKLEAGEQGKSWVEAKDADEQAAELWQHRD
CCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]