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The map label for this gene is rsmB [H]

Identifier: 160873155

GI number: 160873155

Start: 33950

End: 35236

Strand: Reverse

Name: rsmB [H]

Synonym: Sbal195_0029

Alternate gene names: 160873155

Gene position: 35236-33950 (Counterclockwise)

Preceding gene: 160873156

Following gene: 160873154

Centisome position: 0.66

GC content: 50.89

Gene sequence:

>1287_bases
ATGAACTTGCGCGCGCTGGCTGCGAAAGCCATCTTCGAAGTATTAGAAAAAGGCGTGTCACTCTCAGTGGCACTGCCAGA
TCAACAAAAGCACCTTGCCAGCGGCAAAGATAAAGCCCTACTCGCCGAGCTCTGTTACGGCGTGATGCGCACTCTGCCGC
AAATAGAGAAACGCATCAGTGAATGCCTAGCGAAACCACTCAAAGGTAAGCAAAGGATTATCCACCAGCTGCTGATCATA
GGTTGCTATCAACTTTACTTCACTCGCATTCCAAGCCACGCCGCGATTTCAGAAACCGCCGAAGCCTGTCGCCAGTTAAA
ATTTGAAGGCATGGTCAAGGTCGTTAACGGTGTACTGCGCAACATTCAGCGCCAGCTTTCGCCGCTGAGTACTGAGTCAG
ATACCTTAAGCTATAACACGCCCGCTTGGTTGATTAAGCGCTTGAAAGAAGCCTATCCCGAGCAGTGGCAAGATATCATT
CAACAAAGCCATGAACGCCCACCTATGTGGCTGCGTAATAATCGCTTATTCCAGAGCCGTAGCGAATATCTTGCCACCTT
AGCCGAACTCGAGATTGAAGCGAGCGAAGGCAGCAGTGCCGATGCCATCCTTTTAGCGCATCCAAAAGATGTCGCCACAC
TGCCACGCTTCCACGAAGGCGCGGCCTCAGTGCAAGATGGCGCGGCGCAATGGGCGGCGACATTACTGACGCCACAAGCG
AATGAATTAATACTCGATGCCTGCGCGGCGCCCGGCGGTAAAAGCTGTCATCTACTCGAACTTGAGCCAAGTATCAAACT
GGTTGCCGTCGACTTTGATGCCAAACGCCTCGAGCGCGTACAACAGAACCTTGACCGCTTGTCATTAAAAGCGGAGCTTA
TCCATGGCGATGCGGCCAATATTGACTCATGGTGGCAAGGTGGACAATTTGATCGTATCTTACTGGACGCGCCATGCTCG
GCCACAGGAGTGATCCGCCGCCATCCCGATATTAAATGGTTACGTAAAAACCATGACATCGAAGAACTGGCTGAACTACA
ACAGCAAATTCTCGATCATTGCTGGAAGTGGCTCAAACCCGGTGGCACGCTTCTTTATGCTACTTGCTCGATTTTACCGC
AGGAAAACCGCGACCAGATCAGTGCGTTTTTAGAGAGAACGGCAGATGCTAAGCTAGACACACTCTCCCAGCAAAATTCG
CCACAGGATATAGGTTGGCAAATCACGCCAGGACAAGACAATATGGATGGGTTCTATTACGCCCGTTTATTAAAAGCGAC
CGATTAG

Upstream 100 bases:

>100_bases
CAGCTGCCGGGGAAAAAGCCGCTCAATGTTGCCGATATTCTCAATGCTCGAGGTGAATGGTTTAGCCCGAATACTCGCCT
CGCAAACGAGGCACAATAGT

Downstream 100 bases:

>100_bases
GAAGTAAGCAAGGCCATGAAAATTATCATATTAGGTGCGGGTCAAGTCGGGGGAACCTTGGCCGAGAATTTGGTAGGTGA
GAATAATGACATCACCATAG

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNLRALAAKAIFEVLEKGVSLSVALPDQQKHLASGKDKALLAELCYGVMRTLPQIEKRISECLAKPLKGKQRIIHQLLII
GCYQLYFTRIPSHAAISETAEACRQLKFEGMVKVVNGVLRNIQRQLSPLSTESDTLSYNTPAWLIKRLKEAYPEQWQDII
QQSHERPPMWLRNNRLFQSRSEYLATLAELEIEASEGSSADAILLAHPKDVATLPRFHEGAASVQDGAAQWAATLLTPQA
NELILDACAAPGGKSCHLLELEPSIKLVAVDFDAKRLERVQQNLDRLSLKAELIHGDAANIDSWWQGGQFDRILLDAPCS
ATGVIRRHPDIKWLRKNHDIEELAELQQQILDHCWKWLKPGGTLLYATCSILPQENRDQISAFLERTADAKLDTLSQQNS
PQDIGWQITPGQDNMDGFYYARLLKATD

Sequences:

>Translated_428_residues
MNLRALAAKAIFEVLEKGVSLSVALPDQQKHLASGKDKALLAELCYGVMRTLPQIEKRISECLAKPLKGKQRIIHQLLII
GCYQLYFTRIPSHAAISETAEACRQLKFEGMVKVVNGVLRNIQRQLSPLSTESDTLSYNTPAWLIKRLKEAYPEQWQDII
QQSHERPPMWLRNNRLFQSRSEYLATLAELEIEASEGSSADAILLAHPKDVATLPRFHEGAASVQDGAAQWAATLLTPQA
NELILDACAAPGGKSCHLLELEPSIKLVAVDFDAKRLERVQQNLDRLSLKAELIHGDAANIDSWWQGGQFDRILLDAPCS
ATGVIRRHPDIKWLRKNHDIEELAELQQQILDHCWKWLKPGGTLLYATCSILPQENRDQISAFLERTADAKLDTLSQQNS
PQDIGWQITPGQDNMDGFYYARLLKATD
>Mature_428_residues
MNLRALAAKAIFEVLEKGVSLSVALPDQQKHLASGKDKALLAELCYGVMRTLPQIEKRISECLAKPLKGKQRIIHQLLII
GCYQLYFTRIPSHAAISETAEACRQLKFEGMVKVVNGVLRNIQRQLSPLSTESDTLSYNTPAWLIKRLKEAYPEQWQDII
QQSHERPPMWLRNNRLFQSRSEYLATLAELEIEASEGSSADAILLAHPKDVATLPRFHEGAASVQDGAAQWAATLLTPQA
NELILDACAAPGGKSCHLLELEPSIKLVAVDFDAKRLERVQQNLDRLSLKAELIHGDAANIDSWWQGGQFDRILLDAPCS
ATGVIRRHPDIKWLRKNHDIEELAELQQQILDHCWKWLKPGGTLLYATCSILPQENRDQISAFLERTADAKLDTLSQQNS
PQDIGWQITPGQDNMDGFYYARLLKATD

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=381, Percent_Identity=26.246719160105, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI76150623, Length=381, Percent_Identity=26.246719160105, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI32698918, Length=226, Percent_Identity=30.0884955752212, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI301336155, Length=203, Percent_Identity=31.0344827586207, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI39995082, Length=203, Percent_Identity=31.0344827586207, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI40316918, Length=194, Percent_Identity=28.8659793814433, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI11545785, Length=176, Percent_Identity=32.3863636363636, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI270288818, Length=195, Percent_Identity=32.3076923076923, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI270288816, Length=187, Percent_Identity=32.0855614973262, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI8922322, Length=195, Percent_Identity=32.3076923076923, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI23199998, Length=187, Percent_Identity=32.0855614973262, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI2367212, Length=427, Percent_Identity=51.9906323185012, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI87081985, Length=206, Percent_Identity=29.126213592233, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17536757, Length=314, Percent_Identity=25.796178343949, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25143471, Length=179, Percent_Identity=34.0782122905028, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71998419, Length=183, Percent_Identity=31.6939890710383, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI115534021, Length=183, Percent_Identity=31.6939890710383, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17509941, Length=191, Percent_Identity=28.7958115183246, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=309, Percent_Identity=26.8608414239482, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6319447, Length=190, Percent_Identity=31.0526315789474, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI22024126, Length=316, Percent_Identity=25.6329113924051, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19920494, Length=175, Percent_Identity=31.4285714285714, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355201, Length=238, Percent_Identity=27.7310924369748, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356513, Length=222, Percent_Identity=28.8288288288288, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24668781, Length=222, Percent_Identity=28.8288288288288, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48052; Mature: 48052

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRALAAKAIFEVLEKGVSLSVALPDQQKHLASGKDKALLAELCYGVMRTLPQIEKRIS
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ECLAKPLKGKQRIIHQLLIIGCYQLYFTRIPSHAAISETAEACRQLKFEGMVKVVNGVLR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIQRQLSPLSTESDTLSYNTPAWLIKRLKEAYPEQWQDIIQQSHERPPMWLRNNRLFQSR
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEECCCHHHHHH
SEYLATLAELEIEASEGSSADAILLAHPKDVATLPRFHEGAASVQDGAAQWAATLLTPQA
HHHHHHHHHHEEECCCCCCCCEEEEECCCCHHHCCCHHCCCHHHHHHHHHHHHHHCCCCH
NELILDACAAPGGKSCHLLELEPSIKLVAVDFDAKRLERVQQNLDRLSLKAELIHGDAAN
HHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IDSWWQGGQFDRILLDAPCSATGVIRRHPDIKWLRKNHDIEELAELQQQILDHCWKWLKP
CHHHHCCCCCCEEEEECCCCCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
GGTLLYATCSILPQENRDQISAFLERTADAKLDTLSQQNSPQDIGWQITPGQDNMDGFYY
CCEEEEEEEHHCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEECCCCCCCCHHHH
ARLLKATD
HHHHHCCC
>Mature Secondary Structure
MNLRALAAKAIFEVLEKGVSLSVALPDQQKHLASGKDKALLAELCYGVMRTLPQIEKRIS
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ECLAKPLKGKQRIIHQLLIIGCYQLYFTRIPSHAAISETAEACRQLKFEGMVKVVNGVLR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIQRQLSPLSTESDTLSYNTPAWLIKRLKEAYPEQWQDIIQQSHERPPMWLRNNRLFQSR
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEECCCHHHHHH
SEYLATLAELEIEASEGSSADAILLAHPKDVATLPRFHEGAASVQDGAAQWAATLLTPQA
HHHHHHHHHHEEECCCCCCCCEEEEECCCCHHHCCCHHCCCHHHHHHHHHHHHHHCCCCH
NELILDACAAPGGKSCHLLELEPSIKLVAVDFDAKRLERVQQNLDRLSLKAELIHGDAAN
HHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IDSWWQGGQFDRILLDAPCSATGVIRRHPDIKWLRKNHDIEELAELQQQILDHCWKWLKP
CHHHHCCCCCCEEEEECCCCCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
GGTLLYATCSILPQENRDQISAFLERTADAKLDTLSQQNSPQDIGWQITPGQDNMDGFYY
CCEEEEEEEHHCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEECCCCCCCCHHHH
ARLLKATD
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]