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The map label for this gene is glyS [H]

Identifier: 160873138

GI number: 160873138

Start: 12342

End: 14411

Strand: Reverse

Name: glyS [H]

Synonym: Sbal195_0012

Alternate gene names: 160873138

Gene position: 14411-12342 (Counterclockwise)

Preceding gene: 160873139

Following gene: 160873136

Centisome position: 0.27

GC content: 47.49

Gene sequence:

>2070_bases
ATGAATTTTGAAAACTTACTCATAGAGTTAGGCACAGAAGAGTTACCACCTAAGTCACTGCGTAAACTGGCTGAATCCTT
TTTAGCTAACTTTACCGAAGAACTCACCAAAGCGGATTTAGCATTTAGCTCAGCAGTTTGGTACGCAGCGCCACGTCGTC
TGGCGATTAAAGTCACTGAACTTGCGCTTGCACAAGCCGACAAAGTGGTTGAGAAACGTGGCCCCGCCGTGAGTTCAGCC
TTTGATGCTGAAGGTAAGCCAACTAAAGCCGCCGAAGGTTGGGCACGTGGTAATGGTATTACTGTTGAGCAAGCGGAACG
TTTAGTGACAGATAAAGGCGAATGGTTAGTCCATAACGCTAAAGTTGAAGGCGTCGAAACAAAAAGTTTGATCGCTGCTA
TGGCTCAACGTGCACTCGATAAATTGCCCATTCCTAAGCCAATGCGTTGGGGTAATAATAAAACCCAATTTATTCGCCCT
GTGCACACCGCGACTATGTTGTTAGGCAGTGAGTTGATTGAAGGGGAATTGCTGGGTATCAAATCGGCACGCACTGTTCG
CGGTCACCGCTTTATGGGACTCAAGCAGTTTGAATTAGCCCATGCTGATCACTACCTTGCCGACCTGAAAGAAAAAGGCA
AAGTCATCGCCGATTACGAAAGCCGTAAAGCATTGATCAAAGCCGATGCCGAAAAAGCCGCCGCTAAGATTGGTGGTACA
GCCGATATCGAAGATAGCCTACTTGAAGAAGTCGCCTCTTTGGTTGAATGGCCAGTGGTATTGACCGCCAGCTTCGAAGA
AAAATTCTTAGCCGTACCGTCTGAAGCTTTGGTCTACACCATGAAGGGCGATCAAAAATACTTCCCAGTCTTTGATGACG
CGGGCAAGTTACTGCCAAACTTTATCTTTGTGACCAACATAGAATCTAAAGATCCTGCGCAAATTATCTCTGGTAACGAG
AAAGTCGTTCGTCCACGTCTTGCCGACGCCGAATTCTTCTTTAATACCGATAAAAAACACACGCTGGAATCACGTTTACC
TAGCTTAGAAACAGTCTTGTTCCAACAGCAGCTGGGTACATTAAAAGACAAAGTAAATCGTATCTCTGCCCTAGCCGCCT
TTATTGCCGAGCAAACTGGCGCGAATGCGGTTGATGCAGCCCGTGCAGGTTTGTTATCTAAAACCGATTTGATGACCAAT
ATGGTGATGGAATTTACCGATACTCAAGGCACTATGGGCATGCATTACGCCCGCCTCGATGGTGAAACCGAAGCCGTTGC
AGTTGCGATGGAAGAGCAATACAAGCCTAAGTTCTCTGGCGATACAGTGCCAAGTGCAGGTGTATCTTGCGCAGTTGCAT
TAGCAGACAAGCTCGATACTTTAGTCGGCATCTTTGGTATTGGCCAAGCACCAAAAGGCGCAGCCGATCCCTTTGCCCTA
CGCCGCGCGGCGATTGGCGTGCTACGTATTATCGTTGAGAACAAACTGCCACTGGATTTAGTAACCCTGATTGCTAAGGC
TCAGGAACTCCACGGTACTAACTTAAGCAACGCAAACGCAGCAGAGGAAGTGCTTGAATTCTTAATGGCTCGTTTCCGTG
CTTGGTATCAAGATAAAGGTATTGGTGTTGATGTGATCTTAGCCGTGCTAGCACGTCGCCCAACACGCCCTGCTGATTTT
GATAGCCGTATCAATGCGGTATCACACTTTAGAAGCCTAGAAGCTTCTAGCGCCCTTGCGGCAGCGAACAAACGAGTATC
CAACATTCTGGCTAAGGTTGAAGGTGCTTTACCGACAACGATCAATGCGAGTCTGTTAACCGAAGCCGCAGAGCAAGCAT
TAGCGGCCAAGTTGAACGAGCTGCAACCACAGTTGGCGCCTTTGTTTGCCAATGCCGATTACCAGCAAGCCTTAACTTTG
TTGGCTGGCCTACGTGAAAGCGTGGATCAGTTCTTCGAAGATGTGATGGTAATGGCAGATGATGAAGCGTTGAAAAACAA
TCGCTTAGCATTGTTGAATAATCTACGCGAGCAGTTCCTGCACGTCGCGGATATATCACTACTGCAATAA

Upstream 100 bases:

>100_bases
TACATCTTACGCGTTCGTACTATGGCGAAAGCGGTCGCTGAATCTTATTATCAGGCACGCGAAGCGCTTGGCTTCCCAAT
GTGTAAGTAGAGGTAAAAAC

Downstream 100 bases:

>100_bases
TGATACTAAAGCTTTTAAAATTATCGATTTATAAAAAACGCGCTCAATAGCGCGTTTTTTATTGCCCTGATTTCTTAAAC
AGCCCAATCAGAACTGCGTC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 689; Mature: 689

Protein sequence:

>689_residues
MNFENLLIELGTEELPPKSLRKLAESFLANFTEELTKADLAFSSAVWYAAPRRLAIKVTELALAQADKVVEKRGPAVSSA
FDAEGKPTKAAEGWARGNGITVEQAERLVTDKGEWLVHNAKVEGVETKSLIAAMAQRALDKLPIPKPMRWGNNKTQFIRP
VHTATMLLGSELIEGELLGIKSARTVRGHRFMGLKQFELAHADHYLADLKEKGKVIADYESRKALIKADAEKAAAKIGGT
ADIEDSLLEEVASLVEWPVVLTASFEEKFLAVPSEALVYTMKGDQKYFPVFDDAGKLLPNFIFVTNIESKDPAQIISGNE
KVVRPRLADAEFFFNTDKKHTLESRLPSLETVLFQQQLGTLKDKVNRISALAAFIAEQTGANAVDAARAGLLSKTDLMTN
MVMEFTDTQGTMGMHYARLDGETEAVAVAMEEQYKPKFSGDTVPSAGVSCAVALADKLDTLVGIFGIGQAPKGAADPFAL
RRAAIGVLRIIVENKLPLDLVTLIAKAQELHGTNLSNANAAEEVLEFLMARFRAWYQDKGIGVDVILAVLARRPTRPADF
DSRINAVSHFRSLEASSALAAANKRVSNILAKVEGALPTTINASLLTEAAEQALAAKLNELQPQLAPLFANADYQQALTL
LAGLRESVDQFFEDVMVMADDEALKNNRLALLNNLREQFLHVADISLLQ

Sequences:

>Translated_689_residues
MNFENLLIELGTEELPPKSLRKLAESFLANFTEELTKADLAFSSAVWYAAPRRLAIKVTELALAQADKVVEKRGPAVSSA
FDAEGKPTKAAEGWARGNGITVEQAERLVTDKGEWLVHNAKVEGVETKSLIAAMAQRALDKLPIPKPMRWGNNKTQFIRP
VHTATMLLGSELIEGELLGIKSARTVRGHRFMGLKQFELAHADHYLADLKEKGKVIADYESRKALIKADAEKAAAKIGGT
ADIEDSLLEEVASLVEWPVVLTASFEEKFLAVPSEALVYTMKGDQKYFPVFDDAGKLLPNFIFVTNIESKDPAQIISGNE
KVVRPRLADAEFFFNTDKKHTLESRLPSLETVLFQQQLGTLKDKVNRISALAAFIAEQTGANAVDAARAGLLSKTDLMTN
MVMEFTDTQGTMGMHYARLDGETEAVAVAMEEQYKPKFSGDTVPSAGVSCAVALADKLDTLVGIFGIGQAPKGAADPFAL
RRAAIGVLRIIVENKLPLDLVTLIAKAQELHGTNLSNANAAEEVLEFLMARFRAWYQDKGIGVDVILAVLARRPTRPADF
DSRINAVSHFRSLEASSALAAANKRVSNILAKVEGALPTTINASLLTEAAEQALAAKLNELQPQLAPLFANADYQQALTL
LAGLRESVDQFFEDVMVMADDEALKNNRLALLNNLREQFLHVADISLLQ
>Mature_689_residues
MNFENLLIELGTEELPPKSLRKLAESFLANFTEELTKADLAFSSAVWYAAPRRLAIKVTELALAQADKVVEKRGPAVSSA
FDAEGKPTKAAEGWARGNGITVEQAERLVTDKGEWLVHNAKVEGVETKSLIAAMAQRALDKLPIPKPMRWGNNKTQFIRP
VHTATMLLGSELIEGELLGIKSARTVRGHRFMGLKQFELAHADHYLADLKEKGKVIADYESRKALIKADAEKAAAKIGGT
ADIEDSLLEEVASLVEWPVVLTASFEEKFLAVPSEALVYTMKGDQKYFPVFDDAGKLLPNFIFVTNIESKDPAQIISGNE
KVVRPRLADAEFFFNTDKKHTLESRLPSLETVLFQQQLGTLKDKVNRISALAAFIAEQTGANAVDAARAGLLSKTDLMTN
MVMEFTDTQGTMGMHYARLDGETEAVAVAMEEQYKPKFSGDTVPSAGVSCAVALADKLDTLVGIFGIGQAPKGAADPFAL
RRAAIGVLRIIVENKLPLDLVTLIAKAQELHGTNLSNANAAEEVLEFLMARFRAWYQDKGIGVDVILAVLARRPTRPADF
DSRINAVSHFRSLEASSALAAANKRVSNILAKVEGALPTTINASLLTEAAEQALAAKLNELQPQLAPLFANADYQQALTL
LAGLRESVDQFFEDVMVMADDEALKNNRLALLNNLREQFLHVADISLLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=67.343976777939, Blast_Score=931, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75148; Mature: 75148

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFENLLIELGTEELPPKSLRKLAESFLANFTEELTKADLAFSSAVWYAAPRRLAIKVTE
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
LALAQADKVVEKRGPAVSSAFDAEGKPTKAAEGWARGNGITVEQAERLVTDKGEWLVHNA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEC
KVEGVETKSLIAAMAQRALDKLPIPKPMRWGNNKTQFIRPVHTATMLLGSELIEGELLGI
EECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCC
KSARTVRGHRFMGLKQFELAHADHYLADLKEKGKVIADYESRKALIKADAEKAAAKIGGT
CCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCCC
ADIEDSLLEEVASLVEWPVVLTASFEEKFLAVPSEALVYTMKGDQKYFPVFDDAGKLLPN
CCHHHHHHHHHHHHHHCCEEEEECCCHHHHCCCCCCEEEEEECCCEECCCCCCHHHHCCC
FIFVTNIESKDPAQIISGNEKVVRPRLADAEFFFNTDKKHTLESRLPSLETVLFQQQLGT
EEEEEECCCCCCHHHHCCCCCEECCCCCCCHHEECCCCHHHHHHHCCHHHHHHHHHHHHH
LKDKVNRISALAAFIAEQTGANAVDAARAGLLSKTDLMTNMVMEFTDTQGTMGMHYARLD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECC
GETEAVAVAMEEQYKPKFSGDTVPSAGVSCAVALADKLDTLVGIFGIGQAPKGAADPFAL
CCCHHEEEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAAIGVLRIIVENKLPLDLVTLIAKAQELHGTNLSNANAAEEVLEFLMARFRAWYQDKG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
IGVDVILAVLARRPTRPADFDSRINAVSHFRSLEASSALAAANKRVSNILAKVEGALPTT
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
INASLLTEAAEQALAAKLNELQPQLAPLFANADYQQALTLLAGLRESVDQFFEDVMVMAD
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DEALKNNRLALLNNLREQFLHVADISLLQ
CHHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFENLLIELGTEELPPKSLRKLAESFLANFTEELTKADLAFSSAVWYAAPRRLAIKVTE
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
LALAQADKVVEKRGPAVSSAFDAEGKPTKAAEGWARGNGITVEQAERLVTDKGEWLVHNA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEC
KVEGVETKSLIAAMAQRALDKLPIPKPMRWGNNKTQFIRPVHTATMLLGSELIEGELLGI
EECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCC
KSARTVRGHRFMGLKQFELAHADHYLADLKEKGKVIADYESRKALIKADAEKAAAKIGGT
CCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCCC
ADIEDSLLEEVASLVEWPVVLTASFEEKFLAVPSEALVYTMKGDQKYFPVFDDAGKLLPN
CCHHHHHHHHHHHHHHCCEEEEECCCHHHHCCCCCCEEEEEECCCEECCCCCCHHHHCCC
FIFVTNIESKDPAQIISGNEKVVRPRLADAEFFFNTDKKHTLESRLPSLETVLFQQQLGT
EEEEEECCCCCCHHHHCCCCCEECCCCCCCHHEECCCCHHHHHHHCCHHHHHHHHHHHHH
LKDKVNRISALAAFIAEQTGANAVDAARAGLLSKTDLMTNMVMEFTDTQGTMGMHYARLD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECC
GETEAVAVAMEEQYKPKFSGDTVPSAGVSCAVALADKLDTLVGIFGIGQAPKGAADPFAL
CCCHHEEEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAAIGVLRIIVENKLPLDLVTLIAKAQELHGTNLSNANAAEEVLEFLMARFRAWYQDKG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
IGVDVILAVLARRPTRPADFDSRINAVSHFRSLEASSALAAANKRVSNILAKVEGALPTT
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
INASLLTEAAEQALAAKLNELQPQLAPLFANADYQQALTLLAGLRESVDQFFEDVMVMAD
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DEALKNNRLALLNNLREQFLHVADISLLQ
CHHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA