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The map label for this gene is yyaF

Identifier: 16081144

GI number: 16081144

Start: 4199843

End: 4200943

Strand: Reverse

Name: yyaF

Synonym: BSU40920

Alternate gene names: 16081144

Gene position: 4200943-4199843 (Counterclockwise)

Preceding gene: 16081145

Following gene: 16081143

Centisome position: 99.65

GC content: 45.69

Gene sequence:

>1101_bases
ATGGCTTTAACAGCTGGAATTGTTGGTTTGCCGAACGTCGGAAAATCAACACTATTTAACGCAATTACACAGGCCGGTGC
CGAATCAGCCAACTACCCGTTCTGTACAATTGATCCGAACGTGGGAATTGTAGAGGTGCCGGATGATCGTCTGCAAAAGC
TGACTGAGCTGGTCAACCCGAAGAAGACTGTGCCGACTGCTTTTGAATTCACTGATATTGCAGGGATTGTAAAGGGCGCA
TCTAAAGGAGAAGGACTCGGCAACAAGTTCCTATCTCATATTCGCCAAGTTGACGCGATTTGCCACGTTGTCCGTGCTTT
TTCCGATGACAACATTACACACGTTTCCGGAAAGGTTGATCCGATTGATGACATTGAAACGATCAACCTTGAGCTGATCC
TCGCTGATATGGAAACAGTTGAAAAGCGGATTACACGCGTCAGCAAGCTTGCGAAGCAAAAAGATAAAGACGCTGTATTT
GAGTTTGAGATTTTATCTAAACTGAAAGAAGCGTTTGAGAGTGAAAAGCCTGCTCGATCTGTAGAATTCACTGAAGAGCA
GCAAAAGCTTGTGAAGCAGCTCCATTTACTGACTTCGAAGCCTATTTTGTACGTGGCCAACGTAAGTGAAGATGAAGTTG
CTGATCCATCTGGCAATGAAAATGTAGCGAAGATTCGCGAGTACGCTGCCGGCGAAAACGCAGAAGTTATCGTTGTGTGT
GCAAAAATCGAATCTGAAATCGCTGAGCTTGAAGGCGAAGAAAAGCAAATGTTCTTGGAAGAGCTGGGCATTCAAGAATC
AGGTCTTGATCAGCTGATCAAAGCGTCTTACTCTCTTCTCGGCCTCGCAACATATTTCACAGCCGGAGAACAGGAAGTCA
GAGCTTGGACATTCAAAAAAGGCATGAAAGCCCCTGAGTGTGCAGGAATTATTCATAGTGACTTTGAGCGGGGCTTCATT
CGTGCTGAGACAGTTGCTTATGAGGATCTTCTTGCGGGCGGCGGTATGGCAGGAGCTAAAGAGGCAGGAAAAGTCCGCCT
TGAAGGGAAAGAATATGTGGTCCAAGACGGAGATGTTATTCATTTCCGATTTAATGTATAG

Upstream 100 bases:

>100_bases
CTTTTTTGTCAGCTTGTCTTTTCCAGTGAACCTCCTTATAATTGCTCTAGGTTCAAAATACGTTCATACAATGACGGTTT
TATATAGAGGAGATGAAAGA

Downstream 100 bases:

>100_bases
GATGCAGTTGTAAAGGGACAAGAGCTTTGGTATAATATAAAATTGTGAGTAATAGAATTATTGCTCCTTGCCCATTATGG
GCCGCTTAGTCCAAAAGGAG

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MALTAGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGIVEVPDDRLQKLTELVNPKKTVPTAFEFTDIAGIVKGA
SKGEGLGNKFLSHIRQVDAICHVVRAFSDDNITHVSGKVDPIDDIETINLELILADMETVEKRITRVSKLAKQKDKDAVF
EFEILSKLKEAFESEKPARSVEFTEEQQKLVKQLHLLTSKPILYVANVSEDEVADPSGNENVAKIREYAAGENAEVIVVC
AKIESEIAELEGEEKQMFLEELGIQESGLDQLIKASYSLLGLATYFTAGEQEVRAWTFKKGMKAPECAGIIHSDFERGFI
RAETVAYEDLLAGGGMAGAKEAGKVRLEGKEYVVQDGDVIHFRFNV

Sequences:

>Translated_366_residues
MALTAGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGIVEVPDDRLQKLTELVNPKKTVPTAFEFTDIAGIVKGA
SKGEGLGNKFLSHIRQVDAICHVVRAFSDDNITHVSGKVDPIDDIETINLELILADMETVEKRITRVSKLAKQKDKDAVF
EFEILSKLKEAFESEKPARSVEFTEEQQKLVKQLHLLTSKPILYVANVSEDEVADPSGNENVAKIREYAAGENAEVIVVC
AKIESEIAELEGEEKQMFLEELGIQESGLDQLIKASYSLLGLATYFTAGEQEVRAWTFKKGMKAPECAGIIHSDFERGFI
RAETVAYEDLLAGGGMAGAKEAGKVRLEGKEYVVQDGDVIHFRFNV
>Mature_365_residues
ALTAGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGIVEVPDDRLQKLTELVNPKKTVPTAFEFTDIAGIVKGAS
KGEGLGNKFLSHIRQVDAICHVVRAFSDDNITHVSGKVDPIDDIETINLELILADMETVEKRITRVSKLAKQKDKDAVFE
FEILSKLKEAFESEKPARSVEFTEEQQKLVKQLHLLTSKPILYVANVSEDEVADPSGNENVAKIREYAAGENAEVIVVCA
KIESEIAELEGEEKQMFLEELGIQESGLDQLIKASYSLLGLATYFTAGEQEVRAWTFKKGMKAPECAGIIHSDFERGFIR
AETVAYEDLLAGGGMAGAKEAGKVRLEGKEYVVQDGDVIHFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI58761500, Length=370, Percent_Identity=45.945945945946, Blast_Score=312, Evalue=2e-85,
Organism=Homo sapiens, GI58761502, Length=216, Percent_Identity=41.2037037037037, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI24308117, Length=111, Percent_Identity=37.8378378378378, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1787454, Length=366, Percent_Identity=55.7377049180328, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1789574, Length=134, Percent_Identity=29.1044776119403, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17509631, Length=366, Percent_Identity=42.896174863388, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI17508313, Length=104, Percent_Identity=34.6153846153846, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319499, Length=374, Percent_Identity=40.9090909090909, Blast_Score=256, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6321773, Length=385, Percent_Identity=36.6233766233766, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6321649, Length=154, Percent_Identity=38.961038961039, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321962, Length=141, Percent_Identity=29.7872340425532, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640873, Length=369, Percent_Identity=42.8184281842818, Blast_Score=290, Evalue=9e-79,
Organism=Drosophila melanogaster, GI24640877, Length=369, Percent_Identity=42.8184281842818, Blast_Score=290, Evalue=9e-79,
Organism=Drosophila melanogaster, GI24640875, Length=369, Percent_Identity=42.8184281842818, Blast_Score=290, Evalue=9e-79,
Organism=Drosophila melanogaster, GI24640879, Length=328, Percent_Identity=40.5487804878049, Blast_Score=234, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24585318, Length=137, Percent_Identity=32.1167883211679, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ENGD_BACSU (P37518)

Other databases:

- EMBL:   D26185
- EMBL:   AL009126
- PIR:   S66016
- RefSeq:   NP_391972.1
- ProteinModelPortal:   P37518
- SMR:   P37518
- EnsemblBacteria:   EBBACT00000001666
- GeneID:   937916
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU40920
- NMPDR:   fig|224308.1.peg.4098
- GenoList:   BSU40920
- GeneTree:   EBGT00050000000577
- HOGENOM:   HBG586249
- OMA:   QAANFPF
- PhylomeDB:   P37518
- ProtClustDB:   PRK09601
- BioCyc:   BSUB:BSU40920-MONOMER
- GO:   GO:0005622
- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192
- Gene3D:   G3DSA:3.10.20.30
- PANTHER:   PTHR23305:SF4
- PIRSF:   PIRSF006641
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00092

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C; SSF81271 TGS-like

EC number: NA

Molecular weight: Translated: 40069; Mature: 39938

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTAGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGIVEVPDDRLQKLTELVNP
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHCC
KKTVPTAFEFTDIAGIVKGASKGEGLGNKFLSHIRQVDAICHVVRAFSDDNITHVSGKVD
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
PIDDIETINLELILADMETVEKRITRVSKLAKQKDKDAVFEFEILSKLKEAFESEKPARS
CCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
VEFTEEQQKLVKQLHLLTSKPILYVANVSEDEVADPSGNENVAKIREYAAGENAEVIVVC
CCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEE
AKIESEIAELEGEEKQMFLEELGIQESGLDQLIKASYSLLGLATYFTAGEQEVRAWTFKK
EHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
GMKAPECAGIIHSDFERGFIRAETVAYEDLLAGGGMAGAKEAGKVRLEGKEYVVQDGDVI
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEECCCEE
HFRFNV
EEEECC
>Mature Secondary Structure 
ALTAGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGIVEVPDDRLQKLTELVNP
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHCC
KKTVPTAFEFTDIAGIVKGASKGEGLGNKFLSHIRQVDAICHVVRAFSDDNITHVSGKVD
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
PIDDIETINLELILADMETVEKRITRVSKLAKQKDKDAVFEFEILSKLKEAFESEKPARS
CCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
VEFTEEQQKLVKQLHLLTSKPILYVANVSEDEVADPSGNENVAKIREYAAGENAEVIVVC
CCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEE
AKIESEIAELEGEEKQMFLEELGIQESGLDQLIKASYSLLGLATYFTAGEQEVRAWTFKK
EHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
GMKAPECAGIIHSDFERGFIRAETVAYEDLLAGGGMAGAKEAGKVRLEGKEYVVQDGDVI
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEECCCEE
HFRFNV
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377