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The map label for this gene is bglA

Identifier: 16081063

GI number: 16081063

Start: 4121166

End: 4122605

Strand: Reverse

Name: bglA

Synonym: BSU40110

Alternate gene names: 16081063

Gene position: 4122605-4121166 (Counterclockwise)

Preceding gene: 16081064

Following gene: 255767865

Centisome position: 97.79

GC content: 45.28

Gene sequence:

>1440_bases
ATGGGAAACATGCCAAAGGATTTTTTATGGGGCGGTGCTTTAGCTGCCCACCAATTTGAAGGCGGATGGAATCAAGGCGG
TAAAGGGCCGAGTGTCGTCGATGTGATGACAGCGGGTGCGCACGGCGTGCCGAGAAAAATCACTGACACGATTGAAGAAA
ACGAATTTTATCCAAACCATGAAGCCATTGATTTCTATCACAGATACAAGGAAGATATCGCTTTGTTTGCGGAAATGGGA
TTAAAATGTCTGCGGACGTCCATCGGCTGGAGCCGGATTTTTCCGAAAGGCGATGAGGCTGAACCGAACGAAGCTGGTTT
GCAGTTTTATGATGATGTGTTTGATGAATTGCTGAAGCATGGGATCGAGCCGGTCATTACCCTGTCTCACTTTGAAATGC
CGCTGCACCTGGCAAGAGAATATGGCGGCTTCAGAAACCGGAAAGTCGTGGACTTCTTCGTCAACTTCGCAGAGGCTTGC
TTTACCCGTTACAAAGATAAAGTGAAGTACTGGATGACATTTAACGAGATTAACAACCAAATGGATGTGAACAATCCGCT
GTTCCTATGGACGAATTCAGGTGTTGTGGTTGGCGAAAACGAAAACGCCAAGGAAGTCATGTATCAAACGGCGCACCATG
AATTAGTGGCGAGCGCCTTAGCAGTGGCAAAAGGAAAAGACATCAATCCAGAGTTCCAGATTGGGGCCATGGTATCACAC
GTGCCGATTTATCCTTTCTCCTCCAATCCTGAAGATGTGATGCTGGCTGAAGAAGAAATGAGGCAGCGCTATTTCTTCCC
TGATGTGCAGGTTCGCGGTTATTATCCAAGCTACGCGTTGAAGGAATTTGAACGCGAAGGCTACAACATCACATTCGAAG
ACGGCGATGATGAGATTTTAAGAAACGGAACCGTCGATTACTTAGGCTTCAGCTATTACATGTCAACAACTGTGAAAAGC
GATGTGAAAAATGATAACACTGGCGATATTGTAAATGGCGGCTTGCCGAATGGTGTCGAAAATCCGTACATCACTTCGAG
TGACTGGGGATGGGCGATTGATCCAACCGGGTTGAGATATACGTTAAATCGTTTTTATGACCGATACCAAATCCCGTTAT
TCATCGTTGAAAATGGCTTTGGCGCAGTGGATACGCTGGAGGAGGACGGCAAGGTTCATGATCCGGAACGCATTCAATAT
TTAAAATCGCATATTGAAGCATTGAAAAAAGCGGTCACGTACGATGGTGTTGATTTAATTGGCTACACGCCGTGGGGCAT
TATTGATATTGTCTCCTTCACAACTGGCGAAATGAAAAAACGCTACGGCATGATCTATGTTGATCGTGACAATGAAGGAA
ACGGATCCATGAAACGATATAAGAAAGACTCCTTTGAGTGGTATAAAAATGTCATTCAAACGAATGGTGAGGAACTATAA

Upstream 100 bases:

>100_bases
GAAGTGCTTGTTGAGTTTGATAAAGCTTTCATTGAAGACATTGAGGAAAGTGCATTACATCAAAAAATATTTACAGTCGT
AAGCTGAGGAGGAATTAAAC

Downstream 100 bases:

>100_bases
GAAGCGCTCGAATCGAGCACTTTTACCGTAGTGCCTGGTTCGGTTTTTTTCTGTAGAAAAAGAAAAATCCGCTATCCTGC
CAATCTGGCAATATAGCGGA

Product: aryl-6-phospho-beta-glucosidase

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MGNMPKDFLWGGALAAHQFEGGWNQGGKGPSVVDVMTAGAHGVPRKITDTIEENEFYPNHEAIDFYHRYKEDIALFAEMG
LKCLRTSIGWSRIFPKGDEAEPNEAGLQFYDDVFDELLKHGIEPVITLSHFEMPLHLAREYGGFRNRKVVDFFVNFAEAC
FTRYKDKVKYWMTFNEINNQMDVNNPLFLWTNSGVVVGENENAKEVMYQTAHHELVASALAVAKGKDINPEFQIGAMVSH
VPIYPFSSNPEDVMLAEEEMRQRYFFPDVQVRGYYPSYALKEFEREGYNITFEDGDDEILRNGTVDYLGFSYYMSTTVKS
DVKNDNTGDIVNGGLPNGVENPYITSSDWGWAIDPTGLRYTLNRFYDRYQIPLFIVENGFGAVDTLEEDGKVHDPERIQY
LKSHIEALKKAVTYDGVDLIGYTPWGIIDIVSFTTGEMKKRYGMIYVDRDNEGNGSMKRYKKDSFEWYKNVIQTNGEEL

Sequences:

>Translated_479_residues
MGNMPKDFLWGGALAAHQFEGGWNQGGKGPSVVDVMTAGAHGVPRKITDTIEENEFYPNHEAIDFYHRYKEDIALFAEMG
LKCLRTSIGWSRIFPKGDEAEPNEAGLQFYDDVFDELLKHGIEPVITLSHFEMPLHLAREYGGFRNRKVVDFFVNFAEAC
FTRYKDKVKYWMTFNEINNQMDVNNPLFLWTNSGVVVGENENAKEVMYQTAHHELVASALAVAKGKDINPEFQIGAMVSH
VPIYPFSSNPEDVMLAEEEMRQRYFFPDVQVRGYYPSYALKEFEREGYNITFEDGDDEILRNGTVDYLGFSYYMSTTVKS
DVKNDNTGDIVNGGLPNGVENPYITSSDWGWAIDPTGLRYTLNRFYDRYQIPLFIVENGFGAVDTLEEDGKVHDPERIQY
LKSHIEALKKAVTYDGVDLIGYTPWGIIDIVSFTTGEMKKRYGMIYVDRDNEGNGSMKRYKKDSFEWYKNVIQTNGEEL
>Mature_478_residues
GNMPKDFLWGGALAAHQFEGGWNQGGKGPSVVDVMTAGAHGVPRKITDTIEENEFYPNHEAIDFYHRYKEDIALFAEMGL
KCLRTSIGWSRIFPKGDEAEPNEAGLQFYDDVFDELLKHGIEPVITLSHFEMPLHLAREYGGFRNRKVVDFFVNFAEACF
TRYKDKVKYWMTFNEINNQMDVNNPLFLWTNSGVVVGENENAKEVMYQTAHHELVASALAVAKGKDINPEFQIGAMVSHV
PIYPFSSNPEDVMLAEEEMRQRYFFPDVQVRGYYPSYALKEFEREGYNITFEDGDDEILRNGTVDYLGFSYYMSTTVKSD
VKNDNTGDIVNGGLPNGVENPYITSSDWGWAIDPTGLRYTLNRFYDRYQIPLFIVENGFGAVDTLEEDGKVHDPERIQYL
KSHIEALKKAVTYDGVDLIGYTPWGIIDIVSFTTGEMKKRYGMIYVDRDNEGNGSMKRYKKDSFEWYKNVIQTNGEEL

Specific function: Catalyzes the hydrolysis of aryl-phospho-beta-D- glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. Bgl

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family

Homologues:

Organism=Homo sapiens, GI110681710, Length=501, Percent_Identity=28.9421157684631, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI13273313, Length=491, Percent_Identity=27.0875763747454, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI32481206, Length=500, Percent_Identity=28, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI28376633, Length=493, Percent_Identity=27.3833671399594, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI24497614, Length=495, Percent_Identity=26.6666666666667, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI2367174, Length=477, Percent_Identity=65.8280922431866, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1789070, Length=483, Percent_Identity=50.7246376811594, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI2367270, Length=479, Percent_Identity=49.6868475991649, Blast_Score=458, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17552856, Length=486, Percent_Identity=30.0411522633745, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17539390, Length=506, Percent_Identity=28.0632411067194, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21356577, Length=503, Percent_Identity=27.6341948310139, Blast_Score=149, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BGLA_BACSU (P42973)

Other databases:

- EMBL:   L19710
- EMBL:   D78193
- EMBL:   AL009126
- PIR:   I39953
- RefSeq:   NP_391891.1
- ProteinModelPortal:   P42973
- SMR:   P42973
- EnsemblBacteria:   EBBACT00000000121
- GeneID:   937718
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU40110
- NMPDR:   fig|224308.1.peg.4017
- GenoList:   BSU40110
- GeneTree:   EBGT00050000000632
- HOGENOM:   HBG316462
- OMA:   GENENAK
- PhylomeDB:   P42973
- ProtClustDB:   CLSK884519
- BioCyc:   BSUB:BSU40110-MONOMER
- BRENDA:   3.2.1.86
- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781
- Gene3D:   G3DSA:3.20.20.80
- PANTHER:   PTHR10353
- PRINTS:   PR00131

Pfam domain/function: PF00232 Glyco_hydro_1; SSF51445 Glyco_hydro_cat

EC number: =3.2.1.86

Molecular weight: Translated: 54842; Mature: 54711

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: ACT_SITE 176-176 ACT_SITE 377-377

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNMPKDFLWGGALAAHQFEGGWNQGGKGPSVVDVMTAGAHGVPRKITDTIEENEFYPNH
CCCCCCHHHHCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCC
EAIDFYHRYKEDIALFAEMGLKCLRTSIGWSRIFPKGDEAEPNEAGLQFYDDVFDELLKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GIEPVITLSHFEMPLHLAREYGGFRNRKVVDFFVNFAEACFTRYKDKVKYWMTFNEINNQ
CCCEEEEEHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHCCC
MDVNNPLFLWTNSGVVVGENENAKEVMYQTAHHELVASALAVAKGKDINPEFQIGAMVSH
CCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHC
VPIYPFSSNPEDVMLAEEEMRQRYFFPDVQVRGYYPSYALKEFEREGYNITFEDGDDEIL
CEEECCCCCCCCEEECHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCEEEEECCCHHHH
RNGTVDYLGFSYYMSTTVKSDVKNDNTGDIVNGGLPNGVENPYITSSDWGWAIDPTGLRY
HCCCCHHEEHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEECCCCCEEECCCCHHH
TLNRFYDRYQIPLFIVENGFGAVDTLEEDGKVHDPERIQYLKSHIEALKKAVTYDGVDLI
HHHHHHHHHCCEEEEEECCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
GYTPWGIIDIVSFTTGEMKKRYGMIYVDRDNEGNGSMKRYKKDSFEWYKNVIQTNGEEL
CCCCCCHHHHHHCCHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GNMPKDFLWGGALAAHQFEGGWNQGGKGPSVVDVMTAGAHGVPRKITDTIEENEFYPNH
CCCCCHHHHCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCC
EAIDFYHRYKEDIALFAEMGLKCLRTSIGWSRIFPKGDEAEPNEAGLQFYDDVFDELLKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GIEPVITLSHFEMPLHLAREYGGFRNRKVVDFFVNFAEACFTRYKDKVKYWMTFNEINNQ
CCCEEEEEHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHCCC
MDVNNPLFLWTNSGVVVGENENAKEVMYQTAHHELVASALAVAKGKDINPEFQIGAMVSH
CCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHC
VPIYPFSSNPEDVMLAEEEMRQRYFFPDVQVRGYYPSYALKEFEREGYNITFEDGDDEIL
CEEECCCCCCCCEEECHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCEEEEECCCHHHH
RNGTVDYLGFSYYMSTTVKSDVKNDNTGDIVNGGLPNGVENPYITSSDWGWAIDPTGLRY
HCCCCHHEEHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEECCCCCEEECCCCHHH
TLNRFYDRYQIPLFIVENGFGAVDTLEEDGKVHDPERIQYLKSHIEALKKAVTYDGVDLI
HHHHHHHHHCCEEEEEECCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
GYTPWGIIDIVSFTTGEMKKRYGMIYVDRDNEGNGSMKRYKKDSFEWYKNVIQTNGEEL
CCCCCCHHHHHHCCHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8125345; 9384377