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The map label for this gene is ywbI

Identifier: 16080882

GI number: 16080882

Start: 3932198

End: 3933103

Strand: Reverse

Name: ywbI

Synonym: BSU38310

Alternate gene names: 16080882

Gene position: 3933103-3932198 (Counterclockwise)

Preceding gene: 16080887

Following gene: 16080881

Centisome position: 93.3

GC content: 45.92

Gene sequence:

>906_bases
ATGGATATCCGCCATTTAACATATTTCTTGGAAGTCGCCCGTTTAAAAAGTTTTACGAAGGCTTCCCAATCCCTTTATGT
TTCTCAGCCGACGATATCAAAAATGATCAAAAATTTAGAAGAAGAGCTCGGAATTGAACTGTTTTACCGAAACGGCAGGC
AGGTGGAACTGACTGATGCCGGTCATAGCATGTATGTGCAGGCGCAGGAAATCATTAAATCATTTCAAAACCTGACGTCA
GAATTAAATGATATTATGGAAGTCAAGAAAGGGCACGTACGGATTGGACTGCCGCCGATGATCGGATCGGGGTTTTTCCC
CAGAGTGCTCGGCGATTTCCGTGAAAACTATCCGAATGTCACCTTTCAGCTAGTCGAAGACGGTTCTATAAAAGTCCAAG
AAGGTGTCGGGGACGGCTCTCTTGATATCGGCGTTGTGGTCCTTCCCGCAAACGAAGATATCTTTCATTCGTTTACAATC
GTAAAAGAAACCTTAATGCTTGTCGTTCACCCTTCCCACCGGCTGGCTGATGAGAAAGAATGTCAGCTGCGTGAGTTGAA
GGACGAGCCTTTTATTTTCTTCAGAGAGGATTTTGTCCTGCACAACCGAATTATGACGGAATGTATCAAGGCTGGGTTCC
GGCCCCATATCATTTATGAAACATCACAATGGGATTTTATTAGTGAGATGGTATCCGCCAACCTTGGGATCGGGCTTTTG
CCGGAACGGATCTGCCGCGGCCTTGACCCGGAAAAAGTGAAAGTCATTCCGCTTGTCGATCCGGTCATCCCTTGGCACCT
CGCCATTATTTGGAGAAAGGACAGGTACTTGTCGTTTGCCGCCAGAGCTTGGCTCGAACATACGAAATCGTATTTATGGG
ACCCGAAAAAAGATAGTAAAGGATGA

Upstream 100 bases:

>100_bases
TGCCTCCCTTATATACTTATTGTACATAAGGTCTCTCTATAGGTAAAATATATATTAAGAATAAGAAGTATTCCTTTTGT
CTATAGAAAGGGTGAAGCGC

Downstream 100 bases:

>100_bases
TGCAAATATGGATGCACAATCAGCAGCAAAATGTCTTACGGCTGTCCGCCGGCATAGCCCACTGGTGCATAGCATAACCA
ACAATGTCGTAACGAATTTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDIRHLTYFLEVARLKSFTKASQSLYVSQPTISKMIKNLEEELGIELFYRNGRQVELTDAGHSMYVQAQEIIKSFQNLTS
ELNDIMEVKKGHVRIGLPPMIGSGFFPRVLGDFRENYPNVTFQLVEDGSIKVQEGVGDGSLDIGVVVLPANEDIFHSFTI
VKETLMLVVHPSHRLADEKECQLRELKDEPFIFFREDFVLHNRIMTECIKAGFRPHIIYETSQWDFISEMVSANLGIGLL
PERICRGLDPEKVKVIPLVDPVIPWHLAIIWRKDRYLSFAARAWLEHTKSYLWDPKKDSKG

Sequences:

>Translated_301_residues
MDIRHLTYFLEVARLKSFTKASQSLYVSQPTISKMIKNLEEELGIELFYRNGRQVELTDAGHSMYVQAQEIIKSFQNLTS
ELNDIMEVKKGHVRIGLPPMIGSGFFPRVLGDFRENYPNVTFQLVEDGSIKVQEGVGDGSLDIGVVVLPANEDIFHSFTI
VKETLMLVVHPSHRLADEKECQLRELKDEPFIFFREDFVLHNRIMTECIKAGFRPHIIYETSQWDFISEMVSANLGIGLL
PERICRGLDPEKVKVIPLVDPVIPWHLAIIWRKDRYLSFAARAWLEHTKSYLWDPKKDSKG
>Mature_301_residues
MDIRHLTYFLEVARLKSFTKASQSLYVSQPTISKMIKNLEEELGIELFYRNGRQVELTDAGHSMYVQAQEIIKSFQNLTS
ELNDIMEVKKGHVRIGLPPMIGSGFFPRVLGDFRENYPNVTFQLVEDGSIKVQEGVGDGSLDIGVVVLPANEDIFHSFTI
VKETLMLVVHPSHRLADEKECQLRELKDEPFIFFREDFVLHNRIMTECIKAGFRPHIIYETSQWDFISEMVSANLGIGLL
PERICRGLDPEKVKVIPLVDPVIPWHLAIIWRKDRYLSFAARAWLEHTKSYLWDPKKDSKG

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI157672245, Length=282, Percent_Identity=31.9148936170213, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI145693105, Length=300, Percent_Identity=29.6666666666667, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790399, Length=297, Percent_Identity=26.2626262626263, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787879, Length=298, Percent_Identity=28.8590604026846, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788748, Length=298, Percent_Identity=26.1744966442953, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788887, Length=278, Percent_Identity=28.0575539568345, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787530, Length=260, Percent_Identity=26.9230769230769, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789506, Length=290, Percent_Identity=23.448275862069, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI2367136, Length=245, Percent_Identity=27.3469387755102, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788297, Length=254, Percent_Identity=25.1968503937008, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=25.3424657534247, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788296, Length=243, Percent_Identity=26.3374485596708, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790208, Length=282, Percent_Identity=25.531914893617, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=31.7241379310345, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786713, Length=285, Percent_Identity=27.719298245614, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081904, Length=179, Percent_Identity=27.9329608938547, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787806, Length=274, Percent_Identity=23.3576642335766, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789204, Length=249, Percent_Identity=24.8995983935743, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787128, Length=141, Percent_Identity=32.6241134751773, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082132, Length=256, Percent_Identity=25.78125, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786401, Length=143, Percent_Identity=29.3706293706294, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788706, Length=160, Percent_Identity=30.625, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789173, Length=133, Percent_Identity=32.3308270676692, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693193, Length=122, Percent_Identity=27.8688524590164, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789440, Length=201, Percent_Identity=23.8805970149254, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI48994962, Length=116, Percent_Identity=30.1724137931034, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87081978, Length=146, Percent_Identity=32.1917808219178, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YWBI_BACSU (P39592)

Other databases:

- EMBL:   X73124
- EMBL:   AL009126
- PIR:   S39679
- RefSeq:   NP_391710.1
- ProteinModelPortal:   P39592
- SMR:   P39592
- EnsemblBacteria:   EBBACT00000000910
- GeneID:   937626
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38310
- NMPDR:   fig|224308.1.peg.3836
- GenoList:   BSU38310
- GeneTree:   EBGT00070000031895
- HOGENOM:   HBG685425
- OMA:   PERICRG
- PhylomeDB:   P39592
- ProtClustDB:   CLSK888104
- BioCyc:   BSUB:BSU38310-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 34678; Mature: 34678

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRHLTYFLEVARLKSFTKASQSLYVSQPTISKMIKNLEEELGIELFYRNGRQVELTDA
CCHHHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHCCEEEEECCCCEEEEECC
GHSMYVQAQEIIKSFQNLTSELNDIMEVKKGHVRIGLPPMIGSGFFPRVLGDFRENYPNV
CCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCE
TFQLVEDGSIKVQEGVGDGSLDIGVVVLPANEDIFHSFTIVKETLMLVVHPSHRLADEKE
EEEEEECCCEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHEEEECCCHHCCCCHH
CQLRELKDEPFIFFREDFVLHNRIMTECIKAGFRPHIIYETSQWDFISEMVSANLGIGLL
HHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCC
PERICRGLDPEKVKVIPLVDPVIPWHLAIIWRKDRYLSFAARAWLEHTKSYLWDPKKDSK
HHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCC
G
C
>Mature Secondary Structure
MDIRHLTYFLEVARLKSFTKASQSLYVSQPTISKMIKNLEEELGIELFYRNGRQVELTDA
CCHHHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHCCEEEEECCCCEEEEECC
GHSMYVQAQEIIKSFQNLTSELNDIMEVKKGHVRIGLPPMIGSGFFPRVLGDFRENYPNV
CCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCE
TFQLVEDGSIKVQEGVGDGSLDIGVVVLPANEDIFHSFTIVKETLMLVVHPSHRLADEKE
EEEEEECCCEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHEEEECCCHHCCCCHH
CQLRELKDEPFIFFREDFVLHNRIMTECIKAGFRPHIIYETSQWDFISEMVSANLGIGLL
HHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCC
PERICRGLDPEKVKVIPLVDPVIPWHLAIIWRKDRYLSFAARAWLEHTKSYLWDPKKDSK
HHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377