Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is ywfA

Identifier: 16080827

GI number: 16080827

Start: 3874332

End: 3875570

Strand: Reverse

Name: ywfA

Synonym: BSU37750

Alternate gene names: 16080827

Gene position: 3875570-3874332 (Counterclockwise)

Preceding gene: 16080828

Following gene: 16080826

Centisome position: 91.93

GC content: 46.97

Gene sequence:

>1239_bases
ATGAACAGCAATCAAAACAATGACATCAAAACAAAACATCATTTTCCGTTATTGTTGGCACTGGCTTTAACGATGGGGGT
ATTTGCAGCCGGATCTGAAGAGCTTGTGATCTCACCGCTGCTGCCGGATTTAGCAAAAGCCTTCAGCTCAGATGTCAGCG
TCCTTGCGTTATCTATCAGTATTTACGGCGTTATGATTTTTATCGGTGCGCCGCTTTTGGTTCCATTAGGCGATAAATAC
TCCAGAGAACTGAGCTTATTGGCCGGGCTTATGATTTTTATCATAGGAACGGTGATATGCGCTTTAGCTCAAAATATTTT
TTTCTTCTTTTTAGGCCGGGCGCTTTCGGGATTGGCTGCGGGAGCGTTTGTTCCGACCGCTTACGCAGTTGTCGGTGATC
GTGTTCCTTATACATACCGGGGCAAGGTGATGGGACTGATCGTTTCAAGCTGGTCGCTGGCGCTGATTTTCGGAGTGCCG
CTCGGATCATTTATCGGCGGCGTCCTGCATTGGAGATGGACGTTTTGGATTTTCGCCCTGATGGGTGTTTTAGTTGTTCT
GCTGATTCTCCTTGAAATGCGCCGCCATGCCCAACATAAAAACAGCGGCAAGGAAGAAATAGAAGAGCCCGCAGGAACAT
TCCGGGATGCGCTGAAAGTCCCTCGTGTACCTGTTTATATCACAATAACCTTTTGTAATATGATTGGTTTTTACGGTATG
TATTCCTTTTTAGGCACCTATCTTCAGGACGTTTTTACCGGAGGAAACACGGCTGCGGGCTTATTTATCATGATTTATGG
AATCGGGTTTTCCATGAGTGTGATTACAGGAAAGATAGCCGACCGCATCGGAAAAATGCGCTCCCTTTTGATTGCGCTGG
GGGTCATCAGCGTTCTGCTGGCGTGTTTACCATATGCGCCGGCGTCTATGTTTCTGTTAATCGCCAGCCTATTTATTTGG
GGATTAATGCAAAGTTTGACGGTGACGCTGCTCAGCACCATTTTGAGTGACTGCTCAGAGCGTCATCGAGGAAAAGTCAT
GGTGTTTTACAGTCTCGCCTCGAATCTGGCCGTGACATTAGGTTCTGCTTTAATGGGACCCGTATATGTGGCATACGGCT
ATGCAGCTGTCGGATTGATTTGCGCCGCCATCACAGTCCTCGGATTTGTGCTCAGTGTATTCGCATATAAAAAGTACGGC
AAACTTGAGCAGAAGGCCGATCAGTCCTTATCTCAATAA

Upstream 100 bases:

>100_bases
TTATAAATATTTCGTTCGAATTGTAAATAGAGAAAAAACAATGCTAAACTATAATACAACTTTATGATAAGAAGATTATG
GAAGAGGTGTTTGAGAAAGA

Downstream 100 bases:

>100_bases
ACAAAGTTTCTAAATTCCTATAATTAAAAACCCTTTTTAAAGGGTTTTTTTGTTTGTATGGGAATAATTATCAATATTTT
CAAAAAACATTGACAGTTCA

Product: efflux transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MNSNQNNDIKTKHHFPLLLALALTMGVFAAGSEELVISPLLPDLAKAFSSDVSVLALSISIYGVMIFIGAPLLVPLGDKY
SRELSLLAGLMIFIIGTVICALAQNIFFFFLGRALSGLAAGAFVPTAYAVVGDRVPYTYRGKVMGLIVSSWSLALIFGVP
LGSFIGGVLHWRWTFWIFALMGVLVVLLILLEMRRHAQHKNSGKEEIEEPAGTFRDALKVPRVPVYITITFCNMIGFYGM
YSFLGTYLQDVFTGGNTAAGLFIMIYGIGFSMSVITGKIADRIGKMRSLLIALGVISVLLACLPYAPASMFLLIASLFIW
GLMQSLTVTLLSTILSDCSERHRGKVMVFYSLASNLAVTLGSALMGPVYVAYGYAAVGLICAAITVLGFVLSVFAYKKYG
KLEQKADQSLSQ

Sequences:

>Translated_412_residues
MNSNQNNDIKTKHHFPLLLALALTMGVFAAGSEELVISPLLPDLAKAFSSDVSVLALSISIYGVMIFIGAPLLVPLGDKY
SRELSLLAGLMIFIIGTVICALAQNIFFFFLGRALSGLAAGAFVPTAYAVVGDRVPYTYRGKVMGLIVSSWSLALIFGVP
LGSFIGGVLHWRWTFWIFALMGVLVVLLILLEMRRHAQHKNSGKEEIEEPAGTFRDALKVPRVPVYITITFCNMIGFYGM
YSFLGTYLQDVFTGGNTAAGLFIMIYGIGFSMSVITGKIADRIGKMRSLLIALGVISVLLACLPYAPASMFLLIASLFIW
GLMQSLTVTLLSTILSDCSERHRGKVMVFYSLASNLAVTLGSALMGPVYVAYGYAAVGLICAAITVLGFVLSVFAYKKYG
KLEQKADQSLSQ
>Mature_412_residues
MNSNQNNDIKTKHHFPLLLALALTMGVFAAGSEELVISPLLPDLAKAFSSDVSVLALSISIYGVMIFIGAPLLVPLGDKY
SRELSLLAGLMIFIIGTVICALAQNIFFFFLGRALSGLAAGAFVPTAYAVVGDRVPYTYRGKVMGLIVSSWSLALIFGVP
LGSFIGGVLHWRWTFWIFALMGVLVVLLILLEMRRHAQHKNSGKEEIEEPAGTFRDALKVPRVPVYITITFCNMIGFYGM
YSFLGTYLQDVFTGGNTAAGLFIMIYGIGFSMSVITGKIADRIGKMRSLLIALGVISVLLACLPYAPASMFLLIASLFIW
GLMQSLTVTLLSTILSDCSERHRGKVMVFYSLASNLAVTLGSALMGPVYVAYGYAAVGLICAAITVLGFVLSVFAYKKYG
KLEQKADQSLSQ

Specific function: Unknown

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily

Homologues:

Organism=Escherichia coli, GI1787947, Length=314, Percent_Identity=26.4331210191083, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786595, Length=401, Percent_Identity=22.9426433915212, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790146, Length=203, Percent_Identity=26.1083743842365, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193209775, Length=215, Percent_Identity=24.6511627906977, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YWFA_BACSU (P39637)

Other databases:

- EMBL:   X73124
- EMBL:   AL009126
- PIR:   S39734
- RefSeq:   NP_391655.1
- ProteinModelPortal:   P39637
- EnsemblBacteria:   EBBACT00000002884
- GeneID:   937061
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37750
- NMPDR:   fig|224308.1.peg.3781
- GenoList:   BSU37750
- GeneTree:   EBGT00070000031967
- HOGENOM:   HBG338441
- OMA:   FYSLASN
- PhylomeDB:   P39637
- ProtClustDB:   CLSK888075
- BioCyc:   BSUB:BSU37750-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 44550; Mature: 44550

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x126d2e60)-; HASH(0x12c9d528)-; HASH(0x124abd40)-; HASH(0x12c75848)-; HASH(0xffb8894)-; HASH(0x12c2ceb8)-; HASH(0x1320fdd8)-; HASH(0x121730bc)-; HASH(0x1291c69c)-; HASH(0x13256a08)-; HASH(0x12b4b07c)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MNSNQNNDIKTKHHFPLLLALALTMGVFAAGSEELVISPLLPDLAKAFSSDVSVLALSIS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCCHHHHHHHHH
IYGVMIFIGAPLLVPLGDKYSRELSLLAGLMIFIIGTVICALAQNIFFFFLGRALSGLAA
HHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAFVPTAYAVVGDRVPYTYRGKVMGLIVSSWSLALIFGVPLGSFIGGVLHWRWTFWIFAL
HHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
MGVLVVLLILLEMRRHAQHKNSGKEEIEEPAGTFRDALKVPRVPVYITITFCNMIGFYGM
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHH
YSFLGTYLQDVFTGGNTAAGLFIMIYGIGFSMSVITGKIADRIGKMRSLLIALGVISVLL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACLPYAPASMFLLIASLFIWGLMQSLTVTLLSTILSDCSERHRGKVMVFYSLASNLAVTL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHH
GSALMGPVYVAYGYAAVGLICAAITVLGFVLSVFAYKKYGKLEQKADQSLSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCH
>Mature Secondary Structure
MNSNQNNDIKTKHHFPLLLALALTMGVFAAGSEELVISPLLPDLAKAFSSDVSVLALSIS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCCHHHHHHHHH
IYGVMIFIGAPLLVPLGDKYSRELSLLAGLMIFIIGTVICALAQNIFFFFLGRALSGLAA
HHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAFVPTAYAVVGDRVPYTYRGKVMGLIVSSWSLALIFGVPLGSFIGGVLHWRWTFWIFAL
HHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
MGVLVVLLILLEMRRHAQHKNSGKEEIEEPAGTFRDALKVPRVPVYITITFCNMIGFYGM
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHH
YSFLGTYLQDVFTGGNTAAGLFIMIYGIGFSMSVITGKIADRIGKMRSLLIALGVISVLL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACLPYAPASMFLLIASLFIWGLMQSLTVTLLSTILSDCSERHRGKVMVFYSLASNLAVTL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHH
GSALMGPVYVAYGYAAVGLICAAITVLGFVLSVFAYKKYGKLEQKADQSLSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377