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The map label for this gene is cysL

Identifier: 16080817

GI number: 16080817

Start: 3864309

End: 3865208

Strand: Reverse

Name: cysL

Synonym: BSU37650

Alternate gene names: 16080817

Gene position: 3865208-3864309 (Counterclockwise)

Preceding gene: 16080818

Following gene: 16080816

Centisome position: 91.69

GC content: 44.0

Gene sequence:

>900_bases
TTGTATTACGACGTGCTGAAAACGTTTATAGCGGTGGTAGAAGAAAAAAACTTCACAAAAGCGGCGGAAAAGCTGATGAT
CTCCCAGCCCAGCGTCAGTCTGCATATCAAAAATCTTGAGAAAGAATTTCAAACGGCTCTTCTGAACAGGTCGCCAAAAC
ACTTTACGACAACGCCGACAGGAGACATTTTATATCAAAGAGCAAAGCAAATGGTGTTTCTCTACGAACAAGCCAAGGCT
GAAATCTATGCGCATCATCACTATGTGAAGGGTGAATTGAAAATAGCTGCAAGCTTTACAATCGGTGAGTATATTCTTCC
GCCGCTATTGGCCCAGCTGCAAAAGCTGTATCCAGAGCTGAATCTAGATGTCATGATTGGCAATACAGAGGAGGTCAGCG
AGCGTGTGCGGATGCTTCAGGCTGATATCGGGCTGATTGAAGGACATACAAACGAGAATGAGCTGGAAATTGAACCGTTC
ATGGAGGATGAAATGTGCATTGCCGCTCCAAATCAGCATCCGCTCGCCGGGCGGAAAGAGATTTCGATCAGCGATTTGCA
GAACGAAGCGTGGGTGACAAGAGAGAAAGGCTCCGGGACGAGAGAATATCTCGATCATGTACTCAGTTCAAATGGACTTC
GGCCGAAATCAATGTTTACCATCAGCAGCAATCAAGGTGTAAAGGAAGCGGTGATTAACGGCATGGGACTTTCGGTTCTA
TCCAGAAGCGTGCTGCGTAAGGATTTGATCCACCGCGAAATTTCAATTCTGCATATTAATAATTTCTCGTTAAAGCGCAA
GCTGTCTTACATTCATTCTCCTCTCATGGAGAATACAAAAAATAAAGAAATTTTTATTACGATGCTGAAAAGCAATTATC
AATCACAGCTGCTGAAATGA

Upstream 100 bases:

>100_bases
GCGCTTCTGTATAGCCCTTCTTTACCAAGACGGATGATATGAGTACAATAAAGAAAACTAATCATAACCATATATTTCAT
TAATGACAGGGGTGGGGTTT

Downstream 100 bases:

>100_bases
TATAATAGTTTTCGTTCAATAAGCCTAACATGAAAGGGTTTTACATAGATGGCAAACCAACCGATTGATTTACTGATGCA
GCCGAAATGGAGAGTCATTG

Product: regulator of sulfur assimilation CysL, activates cysJI expression

Products: NA

Alternate protein names: CysJI operon transcriptional activator

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MYYDVLKTFIAVVEEKNFTKAAEKLMISQPSVSLHIKNLEKEFQTALLNRSPKHFTTTPTGDILYQRAKQMVFLYEQAKA
EIYAHHHYVKGELKIAASFTIGEYILPPLLAQLQKLYPELNLDVMIGNTEEVSERVRMLQADIGLIEGHTNENELEIEPF
MEDEMCIAAPNQHPLAGRKEISISDLQNEAWVTREKGSGTREYLDHVLSSNGLRPKSMFTISSNQGVKEAVINGMGLSVL
SRSVLRKDLIHREISILHINNFSLKRKLSYIHSPLMENTKNKEIFITMLKSNYQSQLLK

Sequences:

>Translated_299_residues
MYYDVLKTFIAVVEEKNFTKAAEKLMISQPSVSLHIKNLEKEFQTALLNRSPKHFTTTPTGDILYQRAKQMVFLYEQAKA
EIYAHHHYVKGELKIAASFTIGEYILPPLLAQLQKLYPELNLDVMIGNTEEVSERVRMLQADIGLIEGHTNENELEIEPF
MEDEMCIAAPNQHPLAGRKEISISDLQNEAWVTREKGSGTREYLDHVLSSNGLRPKSMFTISSNQGVKEAVINGMGLSVL
SRSVLRKDLIHREISILHINNFSLKRKLSYIHSPLMENTKNKEIFITMLKSNYQSQLLK
>Mature_299_residues
MYYDVLKTFIAVVEEKNFTKAAEKLMISQPSVSLHIKNLEKEFQTALLNRSPKHFTTTPTGDILYQRAKQMVFLYEQAKA
EIYAHHHYVKGELKIAASFTIGEYILPPLLAQLQKLYPELNLDVMIGNTEEVSERVRMLQADIGLIEGHTNENELEIEPF
MEDEMCIAAPNQHPLAGRKEISISDLQNEAWVTREKGSGTREYLDHVLSSNGLRPKSMFTISSNQGVKEAVINGMGLSVL
SRSVLRKDLIHREISILHINNFSLKRKLSYIHSPLMENTKNKEIFITMLKSNYQSQLLK

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788481, Length=267, Percent_Identity=34.4569288389513, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI157672245, Length=232, Percent_Identity=28.8793103448276, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787530, Length=228, Percent_Identity=28.9473684210526, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI145693105, Length=238, Percent_Identity=23.109243697479, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788748, Length=235, Percent_Identity=24.6808510638298, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1789204, Length=277, Percent_Identity=25.6317689530686, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790399, Length=236, Percent_Identity=25.4237288135593, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787879, Length=253, Percent_Identity=28.8537549407115, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786401, Length=253, Percent_Identity=25.6916996047431, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=25.1012145748988, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788296, Length=235, Percent_Identity=24.2553191489362, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1789639, Length=122, Percent_Identity=34.4262295081967, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788887, Length=192, Percent_Identity=27.6041666666667, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI2367136, Length=246, Percent_Identity=26.0162601626016, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082132, Length=252, Percent_Identity=24.2063492063492, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787806, Length=289, Percent_Identity=20.0692041522491, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=189, Percent_Identity=22.2222222222222, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=248, Percent_Identity=25, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789506, Length=237, Percent_Identity=22.3628691983122, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): CYSL_BACSU (P39647)

Other databases:

- EMBL:   X73124
- EMBL:   AL009126
- PIR:   S39744
- RefSeq:   NP_391645.1
- ProteinModelPortal:   P39647
- SMR:   P39647
- EnsemblBacteria:   EBBACT00000002692
- GeneID:   936502
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37650
- NMPDR:   fig|224308.1.peg.3771
- GenoList:   BSU37650
- GeneTree:   EBGT00070000031793
- HOGENOM:   HBG685425
- OMA:   ISSNQGV
- PhylomeDB:   P39647
- ProtClustDB:   CLSK888068
- BioCyc:   BSUB:BSU37650-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 34191; Mature: 34191

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYDVLKTFIAVVEEKNFTKAAEKLMISQPSVSLHIKNLEKEFQTALLNRSPKHFTTTPT
CHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCC
GDILYQRAKQMVFLYEQAKAEIYAHHHYVKGELKIAASFTIGEYILPPLLAQLQKLYPEL
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECEEEEEEEECHHHHHHHHHHHHHHHHCCCC
NLDVMIGNTEEVSERVRMLQADIGLIEGHTNENELEIEPFMEDEMCIAAPNQHPLAGRKE
CEEEEECCHHHHHHHHHHHHHHCCEEECCCCCCCEEECCCCCCCEEEECCCCCCCCCCCC
ISISDLQNEAWVTREKGSGTREYLDHVLSSNGLRPKSMFTISSNQGVKEAVINGMGLSVL
CCHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHCCCCHHHH
SRSVLRKDLIHREISILHINNFSLKRKLSYIHSPLMENTKNKEIFITMLKSNYQSQLLK
HHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHCC
>Mature Secondary Structure
MYYDVLKTFIAVVEEKNFTKAAEKLMISQPSVSLHIKNLEKEFQTALLNRSPKHFTTTPT
CHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCC
GDILYQRAKQMVFLYEQAKAEIYAHHHYVKGELKIAASFTIGEYILPPLLAQLQKLYPEL
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECEEEEEEEECHHHHHHHHHHHHHHHHCCCC
NLDVMIGNTEEVSERVRMLQADIGLIEGHTNENELEIEPFMEDEMCIAAPNQHPLAGRKE
CEEEEECCHHHHHHHHHHHHHHCCEEECCCCCCCEEECCCCCCCEEEECCCCCCCCCCCC
ISISDLQNEAWVTREKGSGTREYLDHVLSSNGLRPKSMFTISSNQGVKEAVINGMGLSVL
CCHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHCCCCHHHH
SRSVLRKDLIHREISILHINNFSLKRKLSYIHSPLMENTKNKEIFITMLKSNYQSQLLK
HHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377