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The map label for this gene is speB

Identifier: 16080801

GI number: 16080801

Start: 3847853

End: 3848725

Strand: Reverse

Name: speB

Synonym: BSU37490

Alternate gene names: 16080801

Gene position: 3848725-3847853 (Counterclockwise)

Preceding gene: 16080802

Following gene: 16080797

Centisome position: 91.3

GC content: 50.63

Gene sequence:

>873_bases
ATGAGATTTGATGAAGCTTATTCAGGAAAAGTATTTATCGCGAGCCGTCCCGAGTGGGAAGAGGCGGACGCCATTCTTTA
CGGCATGCCGATGGACTGGACAGTCAGCTATCGCCCGGGCTCACGCTTTGGTCCAAGCAGAATCCGTGAGGTGTCAATCG
GCCTTGAGGAATACAGCCCATATTTAGACCGCGATCTGGCTGACTTGAACTTCTTTGACGCTGGCGACATTCCGCTGCCG
TTCGGAAATCCGCAGCGAAGCCTGGATATGATTGAAGAGTACGTGGACAGCATTTTAGAAAAAGGGAAGTTCCCAATGGG
TATGGGCGGGGAGCACCTCGTGTCATGGCCTGTCATTAAAGCGATGTACAAGAAATACCCAGACCTTGCGATTATCCATT
TTGACGCACATACAGATCTTCGTGTTGATTATGAGGGAGAACCGCTTTCTCACTCCACGCCGATCCGTAAGGCGGCAGAA
TTGATCGGACCGCACAATGTGTATTCATTCGGAATCCGTTCCGGCATGAAAGAAGAGTTTGAATGGGCGAAGGAAAACGG
CATGCACATCTCAAAATTTGAAGTGCTTGAACCGCTGAAAGAGGTGCTTCCGAAGCTGGCGGGCCGTCCGGTTTATGTCA
CAATTGACATTGACGTATTAGACCCTGCACACGCACCGGGTACAGGAACGGTTGACGCCGGCGGCATCACGTCTAAAGAA
CTGCTTGCATCGGTCCATGAAATTGCGCGTTCAGAGGTAAACGTAAAGGGCGCTGATTTAGTGGAAGTCGCGCCGGTATA
CGACCACTCAGAGCAAACGGCAAACACAGCCAGCAAAATCATCCGTGAAATGCTGCTTGGGTTTGTGAAATAA

Upstream 100 bases:

>100_bases
GTTTGTTCTGCCGAAATTTGTGAGTGACCTGATTAAATAATGAAGGTATGGCGCAGGAGCGAATCTTGCGCTTTTTTACA
GGAAAGCGAGGGTATTATAG

Downstream 100 bases:

>100_bases
GTAAACATCCAGACGATGTCTGGATGTTTTTTTATTCCCAGTCTTTACAAACATCAATCCACACTTTTGAGAAAGAAAAT
TCGGATAATTCTTTCGGTGT

Product: agmatinase

Products: NA

Alternate protein names: Agmatine ureohydrolase; AUH

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRFDEAYSGKVFIASRPEWEEADAILYGMPMDWTVSYRPGSRFGPSRIREVSIGLEEYSPYLDRDLADLNFFDAGDIPLP
FGNPQRSLDMIEEYVDSILEKGKFPMGMGGEHLVSWPVIKAMYKKYPDLAIIHFDAHTDLRVDYEGEPLSHSTPIRKAAE
LIGPHNVYSFGIRSGMKEEFEWAKENGMHISKFEVLEPLKEVLPKLAGRPVYVTIDIDVLDPAHAPGTGTVDAGGITSKE
LLASVHEIARSEVNVKGADLVEVAPVYDHSEQTANTASKIIREMLLGFVK

Sequences:

>Translated_290_residues
MRFDEAYSGKVFIASRPEWEEADAILYGMPMDWTVSYRPGSRFGPSRIREVSIGLEEYSPYLDRDLADLNFFDAGDIPLP
FGNPQRSLDMIEEYVDSILEKGKFPMGMGGEHLVSWPVIKAMYKKYPDLAIIHFDAHTDLRVDYEGEPLSHSTPIRKAAE
LIGPHNVYSFGIRSGMKEEFEWAKENGMHISKFEVLEPLKEVLPKLAGRPVYVTIDIDVLDPAHAPGTGTVDAGGITSKE
LLASVHEIARSEVNVKGADLVEVAPVYDHSEQTANTASKIIREMLLGFVK
>Mature_290_residues
MRFDEAYSGKVFIASRPEWEEADAILYGMPMDWTVSYRPGSRFGPSRIREVSIGLEEYSPYLDRDLADLNFFDAGDIPLP
FGNPQRSLDMIEEYVDSILEKGKFPMGMGGEHLVSWPVIKAMYKKYPDLAIIHFDAHTDLRVDYEGEPLSHSTPIRKAAE
LIGPHNVYSFGIRSGMKEEFEWAKENGMHISKFEVLEPLKEVLPKLAGRPVYVTIDIDVLDPAHAPGTGTVDAGGITSKE
LLASVHEIARSEVNVKGADLVEVAPVYDHSEQTANTASKIIREMLLGFVK

Specific function: Catalyzes the formation of putrescine from agmatine

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family. Agmatinase subfamily

Homologues:

Organism=Homo sapiens, GI37537722, Length=284, Percent_Identity=37.6760563380282, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI10947139, Length=282, Percent_Identity=26.241134751773, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789306, Length=275, Percent_Identity=34.9090909090909, Blast_Score=153, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6325146, Length=229, Percent_Identity=26.6375545851528, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEB_BACSU (P70999)

Other databases:

- EMBL:   Z80360
- EMBL:   AL009126
- PIR:   H70057
- RefSeq:   NP_391629.1
- ProteinModelPortal:   P70999
- SMR:   P70999
- EnsemblBacteria:   EBBACT00000000019
- GeneID:   937205
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37490
- NMPDR:   fig|224308.1.peg.3755
- GenoList:   BSU37490
- GeneTree:   EBGT00050000001627
- HOGENOM:   HBG391953
- OMA:   KIIREML
- PhylomeDB:   P70999
- ProtClustDB:   CLSK888062
- BioCyc:   BSUB:BSU37490-MONOMER
- BioCyc:   MetaCyc:SPEBBACSU-MONOMER
- BRENDA:   3.5.3.11
- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855
- Gene3D:   G3DSA:3.40.800.10
- PANTHER:   PTHR11358
- TIGRFAMs:   TIGR01230

Pfam domain/function: PF00491 Arginase

EC number: =3.5.3.11

Molecular weight: Translated: 32417; Mature: 32417

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01053 ARGINASE_1; PS51409 ARGINASE_2; PS00147 ARGINASE_1 ; PS00148 ARGINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDEAYSGKVFIASRPEWEEADAILYGMPMDWTVSYRPGSRFGPSRIREVSIGLEEYSP
CCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHCCHHHHCC
YLDRDLADLNFFDAGDIPLPFGNPQRSLDMIEEYVDSILEKGKFPMGMGGEHLVSWPVIK
HHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AMYKKYPDLAIIHFDAHTDLRVDYEGEPLSHSTPIRKAAELIGPHNVYSFGIRSGMKEEF
HHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHH
EWAKENGMHISKFEVLEPLKEVLPKLAGRPVYVTIDIDVLDPAHAPGTGTVDAGGITSKE
HHHHHCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHH
LLASVHEIARSEVNVKGADLVEVAPVYDHSEQTANTASKIIREMLLGFVK
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRFDEAYSGKVFIASRPEWEEADAILYGMPMDWTVSYRPGSRFGPSRIREVSIGLEEYSP
CCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHCCHHHHCC
YLDRDLADLNFFDAGDIPLPFGNPQRSLDMIEEYVDSILEKGKFPMGMGGEHLVSWPVIK
HHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AMYKKYPDLAIIHFDAHTDLRVDYEGEPLSHSTPIRKAAELIGPHNVYSFGIRSGMKEEF
HHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHH
EWAKENGMHISKFEVLEPLKEVLPKLAGRPVYVTIDIDVLDPAHAPGTGTVDAGGITSKE
HHHHHCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHH
LLASVHEIARSEVNVKGADLVEVAPVYDHSEQTANTASKIIREMLLGFVK
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377