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The map label for this gene is narH

Identifier: 16080780

GI number: 16080780

Start: 3824806

End: 3826269

Strand: Reverse

Name: narH

Synonym: BSU37270

Alternate gene names: 16080780

Gene position: 3826269-3824806 (Counterclockwise)

Preceding gene: 255767790

Following gene: 16080779

Centisome position: 90.76

GC content: 48.09

Gene sequence:

>1464_bases
TTGAAGATTAAAGCGCAAATCGGTATGGTCATGAACTTGGATAAATGCATCGGCTGCCACACGTGCAGCGTCACCTGCAA
AAACACGTGGACAAACCGTTCCGGTGCGGAATATATGTACTTCAATAATGTAGAAACAAAGCCGGGCATCGGCTACCCGA
AGCAATGGGAGGACCAGGACAAATATAAAGGCGGCTGGACATTGAAAAAAGGAAAGCTCGAGCTGAAATCGGGCTCGAAA
ACCAATCGGCTTGCAGGCCTTTTCTATAATCCGAATCAGCCGTCAATTGATGATTACTATGAACCTTGGAACTATGATTA
TGAAACATTAACGAACAGCCCGCAGAAAAAACACCAGCCGGTAGCACGCCCGAAATCGTCCTTGACGGGGGATTTCATGA
ATATCGAATGGGGACCGAACTGGGAGGACGATCTCGCAGGCGGCCACATTACGGGACTTGAAGATCCCAACGTACAAAAG
ATGGAGGAATCGATCAAAACAGAATTCGATGACGTCTTTATGATGTATTTGCCCCGTATTTGCGAGCACTGCATCAACCC
GGCATGCGTCTCATCCTGTCCATCCGGCGCCATGTACAAACGCGAGGAGGACGGCATTGTGCTTGTGGATCAAAACGCAT
GCCGTTCATGGAGATATTGCGTCTCATCCTGTCCTTATAAAAAAGTCTATTTTAACTGGCAAACGAACAAAGCGGAAAAA
TGCACACTCTGCTTTCCGCGTTTGGAGGCGGGACTGCCAACCATCTGCTCTGAGACGTGTGTTGGCAGAATCCGCTACCT
CGGCGTCATGCTATATGACGCGGACAAAGTGGAGGAAGCGGCATCTGTTGAAAATGAAAAGGATCTCTACCATTCCCAAT
TGGACGTTTTTCTTGATCCGAATGATCCTGAGGTTGCCAAACTGGCAAAAGAACAAGGCATTCCGGCTGAATGGATAGAG
GCCGCGCAGCAATCACCGATCTATAAAATGATCATTGACTGGAAGATCGCGCTGCCGCTTCATCCTGAGTACCGCACGCT
GCCAATGGTGTGGTACATTCCGCCGCTCAGCCCGATTATGAATCTCTTTGAAGGAAAAGGCAGCCGGCAAACGGCGGAAG
ATATTTTTCCGGCTATCGACCAAATGAGAATCCCGATAGATTATTTGGCGCAGCTGTTAACAGCCGGTGATACGGATCAT
ATTCGGTCAACATTAAAGAAAATGTCTGTCATGCGCCAGTATATGAGAGCGGTCCAGACGAATAAATCAATCGATCCGGA
ACTGATCTCCAGTGTCGGCTTAACAGAACAGCAAATTGAAGATATGTATCGGCTGCTTGCGATTGCCAAATATGATGACC
GCTTTGTGATTCCGAGCAGCCATCGAGAAGAAGTATCAGATTTATACGCTGAACAAGGAAGCTGCGGCTTATCATTTTCA
GGCGGCCCCGGCTCCTGTTTCTAA

Upstream 100 bases:

>100_bases
GGCTACGCCCAGCTCAGCTACGGCTTTAATTATTACGGTCCAACGGGGAATCAGCGCGACCTGAACGTCGTCATCCGCAA
GCTGAAGGAGGTCGATTGGC

Downstream 100 bases:

>100_bases
CTTTTGATAAATGAGGTGTAACAAGATGAACACCACAGACCGGCAAATCACGTTCTCTGCTCTTTCCTGTCTTCTCTCTT
ATCCGGATGAAGAGTGGAGA

Product: nitrate reductase beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKIKAQIGMVMNLDKCIGCHTCSVTCKNTWTNRSGAEYMYFNNVETKPGIGYPKQWEDQDKYKGGWTLKKGKLELKSGSK
TNRLAGLFYNPNQPSIDDYYEPWNYDYETLTNSPQKKHQPVARPKSSLTGDFMNIEWGPNWEDDLAGGHITGLEDPNVQK
MEESIKTEFDDVFMMYLPRICEHCINPACVSSCPSGAMYKREEDGIVLVDQNACRSWRYCVSSCPYKKVYFNWQTNKAEK
CTLCFPRLEAGLPTICSETCVGRIRYLGVMLYDADKVEEAASVENEKDLYHSQLDVFLDPNDPEVAKLAKEQGIPAEWIE
AAQQSPIYKMIIDWKIALPLHPEYRTLPMVWYIPPLSPIMNLFEGKGSRQTAEDIFPAIDQMRIPIDYLAQLLTAGDTDH
IRSTLKKMSVMRQYMRAVQTNKSIDPELISSVGLTEQQIEDMYRLLAIAKYDDRFVIPSSHREEVSDLYAEQGSCGLSFS
GGPGSCF

Sequences:

>Translated_487_residues
MKIKAQIGMVMNLDKCIGCHTCSVTCKNTWTNRSGAEYMYFNNVETKPGIGYPKQWEDQDKYKGGWTLKKGKLELKSGSK
TNRLAGLFYNPNQPSIDDYYEPWNYDYETLTNSPQKKHQPVARPKSSLTGDFMNIEWGPNWEDDLAGGHITGLEDPNVQK
MEESIKTEFDDVFMMYLPRICEHCINPACVSSCPSGAMYKREEDGIVLVDQNACRSWRYCVSSCPYKKVYFNWQTNKAEK
CTLCFPRLEAGLPTICSETCVGRIRYLGVMLYDADKVEEAASVENEKDLYHSQLDVFLDPNDPEVAKLAKEQGIPAEWIE
AAQQSPIYKMIIDWKIALPLHPEYRTLPMVWYIPPLSPIMNLFEGKGSRQTAEDIFPAIDQMRIPIDYLAQLLTAGDTDH
IRSTLKKMSVMRQYMRAVQTNKSIDPELISSVGLTEQQIEDMYRLLAIAKYDDRFVIPSSHREEVSDLYAEQGSCGLSFS
GGPGSCF
>Mature_487_residues
MKIKAQIGMVMNLDKCIGCHTCSVTCKNTWTNRSGAEYMYFNNVETKPGIGYPKQWEDQDKYKGGWTLKKGKLELKSGSK
TNRLAGLFYNPNQPSIDDYYEPWNYDYETLTNSPQKKHQPVARPKSSLTGDFMNIEWGPNWEDDLAGGHITGLEDPNVQK
MEESIKTEFDDVFMMYLPRICEHCINPACVSSCPSGAMYKREEDGIVLVDQNACRSWRYCVSSCPYKKVYFNWQTNKAEK
CTLCFPRLEAGLPTICSETCVGRIRYLGVMLYDADKVEEAASVENEKDLYHSQLDVFLDPNDPEVAKLAKEQGIPAEWIE
AAQQSPIYKMIIDWKIALPLHPEYRTLPMVWYIPPLSPIMNLFEGKGSRQTAEDIFPAIDQMRIPIDYLAQLLTAGDTDH
IRSTLKKMSVMRQYMRAVQTNKSIDPELISSVGLTEQQIEDMYRLLAIAKYDDRFVIPSSHREEVSDLYAEQGSCGLSFS
GGPGSCF

Specific function: The Nitrate Reductase Enzyme Complex Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In The Formation Of Iron-Sulfur Centers. Electrons A

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1787478, Length=485, Percent_Identity=59.3814432989691, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=484, Percent_Identity=58.4710743801653, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=131, Percent_Identity=36.6412213740458, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1787122, Length=131, Percent_Identity=36.6412213740458, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367345, Length=134, Percent_Identity=30.5970149253731, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=39.5348837209302, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789370, Length=91, Percent_Identity=38.4615384615385, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790326, Length=135, Percent_Identity=29.6296296296296, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NARH_BACSU (P42176)

Other databases:

- EMBL:   Z49884
- EMBL:   X91819
- EMBL:   X85014
- EMBL:   AL009126
- PIR:   S60086
- RefSeq:   NP_391608.1
- ProteinModelPortal:   P42176
- SMR:   P42176
- EnsemblBacteria:   EBBACT00000003792
- GeneID:   937047
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37270
- NMPDR:   fig|224308.1.peg.3734
- GenoList:   BSU37270
- GeneTree:   EBGT00050000002637
- HOGENOM:   HBG461345
- OMA:   MRIPIDY
- PhylomeDB:   P42176
- ProtClustDB:   CLSK868287
- BioCyc:   BSUB:BSU37270-MONOMER
- BRENDA:   1.7.99.4
- GO:   GO:0006810
- InterPro:   IPR017896
- InterPro:   IPR006547
- TIGRFAMs:   TIGR01660

Pfam domain/function: NA

EC number: =1.7.99.4

Molecular weight: Translated: 55473; Mature: 55473

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKAQIGMVMNLDKCIGCHTCSVTCKNTWTNRSGAEYMYFNNVETKPGIGYPKQWEDQD
CEEHHHHCCEEEHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCH
KYKGGWTLKKGKLELKSGSKTNRLAGLFYNPNQPSIDDYYEPWNYDYETLTNSPQKKHQP
HCCCCEEEECCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCHHHCCC
VARPKSSLTGDFMNIEWGPNWEDDLAGGHITGLEDPNVQKMEESIKTEFDDVFMMYLPRI
CCCCCHHCCCCEEEECCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CEHCINPACVSSCPSGAMYKREEDGIVLVDQNACRSWRYCVSSCPYKKVYFNWQTNKAEK
HHHHCCHHHHHCCCCCCCEEECCCCEEEECCHHHHHHHHHHHCCCCEEEEEEEECCCCCE
CTLCFPRLEAGLPTICSETCVGRIRYLGVMLYDADKVEEAASVENEKDLYHSQLDVFLDP
EEEECHHHHCCCCHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCHHHHHHCCEEEEECC
NDPEVAKLAKEQGIPAEWIEAAQQSPIYKMIIDWKIALPLHPEYRTLPMVWYIPPLSPIM
CCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCHHHHH
NLFEGKGSRQTAEDIFPAIDQMRIPIDYLAQLLTAGDTDHIRSTLKKMSVMRQYMRAVQT
HHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
NKSIDPELISSVGLTEQQIEDMYRLLAIAKYDDRFVIPSSHREEVSDLYAEQGSCGLSFS
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCCEEEC
GGPGSCF
CCCCCCC
>Mature Secondary Structure
MKIKAQIGMVMNLDKCIGCHTCSVTCKNTWTNRSGAEYMYFNNVETKPGIGYPKQWEDQD
CEEHHHHCCEEEHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCH
KYKGGWTLKKGKLELKSGSKTNRLAGLFYNPNQPSIDDYYEPWNYDYETLTNSPQKKHQP
HCCCCEEEECCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCHHHCCC
VARPKSSLTGDFMNIEWGPNWEDDLAGGHITGLEDPNVQKMEESIKTEFDDVFMMYLPRI
CCCCCHHCCCCEEEECCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CEHCINPACVSSCPSGAMYKREEDGIVLVDQNACRSWRYCVSSCPYKKVYFNWQTNKAEK
HHHHCCHHHHHCCCCCCCEEECCCCEEEECCHHHHHHHHHHHCCCCEEEEEEEECCCCCE
CTLCFPRLEAGLPTICSETCVGRIRYLGVMLYDADKVEEAASVENEKDLYHSQLDVFLDP
EEEECHHHHCCCCHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCHHHHHHCCEEEEECC
NDPEVAKLAKEQGIPAEWIEAAQQSPIYKMIIDWKIALPLHPEYRTLPMVWYIPPLSPIM
CCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCHHHHH
NLFEGKGSRQTAEDIFPAIDQMRIPIDYLAQLLTAGDTDHIRSTLKKMSVMRQYMRAVQT
HHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
NKSIDPELISSVGLTEQQIEDMYRLLAIAKYDDRFVIPSSHREEVSDLYAEQGSCGLSFS
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCCEEEC
GGPGSCF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377