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The map label for this gene is clsB

Identifier: 16080772

GI number: 16080772

Start: 3816654

End: 3817850

Strand: Direct

Name: clsB

Synonym: BSU37190

Alternate gene names: 16080772

Gene position: 3816654-3817850 (Clockwise)

Preceding gene: 16080767

Following gene: 16080773

Centisome position: 90.54

GC content: 44.19

Gene sequence:

>1197_bases
ATGAAGGTATTTATCGTGATTATGATCATTGTCGTTATTTTCTTTGCCTTGATTCTGCTTGATATCTTCATGGGCCGTGC
CGGATACAGAAAAAAGGCGTACGAGCCTGTTTTTTCAAAAAAGAAGAGTGACATCGAATTGATCCACTGCGGCGCTGATC
TCGTTGAGCGGATGATGAATGATATTCGCCAGGCCGCCTCTTCTGTTCACATGATGTTTTTTATTATGAAAAATGATGAG
GTCAGCCACAATATGTATACCTTGCTGAAAACAAAAGCCCAAGCTGGCGTATCCGTTTATTTGCTTCTGGATTGGGCGGG
CTGCCGGGCAATTAAAAAAACGGCGCTTCAGACGATGAAAAATGCCGGCGTCCATGTCCATGTGATGAATCGTCCCCGTT
TTCCTTTTTTCTTTTTTCACATGCAAAAACGGAATCACCGGAAGATTACGGTGATAGACGGAAAAATCGGTTATATCGGC
GGGTTTAATATCGCCGAAGAATATCTTGGAAAGAAGGCGAAGTTCGGCAATTGGGAGGATTATCATCTGCGCATGATCGG
AGAAGGCGTACATGATCTTCAAACGCTTTTTGCTTCTGATCTAAAACGAAATACCGGAATCGAACTGGGTTCGGATGTTT
GGCCAAAGCTTCAGCAAGGGACTATTTCTCACAAGATCTATGCGACCGACGGTTATTCCCTTGAAAACATATACTTGGCC
AACATTGCCCAAGCGAAGAATCGCCTTACGGTTTGCACGCCTTATTACATTCCATCAAAACCGCTTCAAGAGGCCCTTAT
TAACGCAAGAAAAAACGGCGTATCCGTGAGAATCATTGTACCGATGAAATCAGACCATCCGCTGGTACGAGAAGCGGCTT
TTACGTATTATTCGGAGCTCTTGGATGCCGGCTGTCTTATTTATCGTTATTATCAAGGGTTTTATCATGTAAAAGCCCTC
ATCATAGACGACCATCTCAGCATTATCGGAACAGCCAACTTCGATAAACGGAGCCTGTTCTTAAATGAAGAGGTGAATGT
TGAAATTGATGACGAAGCGTTTACAAGTGAAGTCTATGCCACAATTGAGGAGGATATGAAAAAGTCGGAGCTGCTGACGA
TGGAGGATTTCAGCAAGCGCACATTCAGGCAGCGCCCGGCGGAATGGCTCGGCAGGGCATTATCTTATTTCTTATAA

Upstream 100 bases:

>100_bases
ATAAGATTATCTTTATGATAAATAATGAATGAGTGTTCAGTCAATAATTAAATTCCTGCTGTTCGGGCACACTACCTTTA
CTACGGAAAGGAGTGCCGGT

Downstream 100 bases:

>100_bases
AGGAGAGTTCCTATGATTTTCAGATTCGGGTTCGTTTCAAATGCGATGAGCCTTTGGGATGCGTCTCCCGCTAAAACATT
GACATTCGCGCGTTATTCAA

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKVFIVIMIIVVIFFALILLDIFMGRAGYRKKAYEPVFSKKKSDIELIHCGADLVERMMNDIRQAASSVHMMFFIMKNDE
VSHNMYTLLKTKAQAGVSVYLLLDWAGCRAIKKTALQTMKNAGVHVHVMNRPRFPFFFFHMQKRNHRKITVIDGKIGYIG
GFNIAEEYLGKKAKFGNWEDYHLRMIGEGVHDLQTLFASDLKRNTGIELGSDVWPKLQQGTISHKIYATDGYSLENIYLA
NIAQAKNRLTVCTPYYIPSKPLQEALINARKNGVSVRIIVPMKSDHPLVREAAFTYYSELLDAGCLIYRYYQGFYHVKAL
IIDDHLSIIGTANFDKRSLFLNEEVNVEIDDEAFTSEVYATIEEDMKKSELLTMEDFSKRTFRQRPAEWLGRALSYFL

Sequences:

>Translated_398_residues
MKVFIVIMIIVVIFFALILLDIFMGRAGYRKKAYEPVFSKKKSDIELIHCGADLVERMMNDIRQAASSVHMMFFIMKNDE
VSHNMYTLLKTKAQAGVSVYLLLDWAGCRAIKKTALQTMKNAGVHVHVMNRPRFPFFFFHMQKRNHRKITVIDGKIGYIG
GFNIAEEYLGKKAKFGNWEDYHLRMIGEGVHDLQTLFASDLKRNTGIELGSDVWPKLQQGTISHKIYATDGYSLENIYLA
NIAQAKNRLTVCTPYYIPSKPLQEALINARKNGVSVRIIVPMKSDHPLVREAAFTYYSELLDAGCLIYRYYQGFYHVKAL
IIDDHLSIIGTANFDKRSLFLNEEVNVEIDDEAFTSEVYATIEEDMKKSELLTMEDFSKRTFRQRPAEWLGRALSYFL
>Mature_398_residues
MKVFIVIMIIVVIFFALILLDIFMGRAGYRKKAYEPVFSKKKSDIELIHCGADLVERMMNDIRQAASSVHMMFFIMKNDE
VSHNMYTLLKTKAQAGVSVYLLLDWAGCRAIKKTALQTMKNAGVHVHVMNRPRFPFFFFHMQKRNHRKITVIDGKIGYIG
GFNIAEEYLGKKAKFGNWEDYHLRMIGEGVHDLQTLFASDLKRNTGIELGSDVWPKLQQGTISHKIYATDGYSLENIYLA
NIAQAKNRLTVCTPYYIPSKPLQEALINARKNGVSVRIIVPMKSDHPLVREAAFTYYSELLDAGCLIYRYYQGFYHVKAL
IIDDHLSIIGTANFDKRSLFLNEEVNVEIDDEAFTSEVYATIEEDMKKSELLTMEDFSKRTFRQRPAEWLGRALSYFL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1787502, Length=360, Percent_Identity=31.1111111111111, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1787007, Length=332, Percent_Identity=28.0120481927711, Blast_Score=121, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YWJE_BACSU (P45865)

Other databases:

- EMBL:   Z49782
- EMBL:   AL009126
- PIR:   S55419
- RefSeq:   NP_391600.1
- ProteinModelPortal:   P45865
- EnsemblBacteria:   EBBACT00000001433
- GeneID:   938460
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37190
- NMPDR:   fig|224308.1.peg.3726
- GenoList:   BSU37190
- GeneTree:   EBGT00070000031955
- HOGENOM:   HBG694312
- OMA:   TANFDKR
- PhylomeDB:   P45865
- ProtClustDB:   CLSK888052
- BioCyc:   BSUB:BSU37190-MONOMER
- InterPro:   IPR001736
- SMART:   SM00155

Pfam domain/function: PF00614 PLDc

EC number: 2.7.8.- [C]

Molecular weight: Translated: 45843; Mature: 45843

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1307a838)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFIVIMIIVVIFFALILLDIFMGRAGYRKKAYEPVFSKKKSDIELIHCGADLVERMMN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCCCCEEEEECCHHHHHHHHH
DIRQAASSVHMMFFIMKNDEVSHNMYTLLKTKAQAGVSVYLLLDWAGCRAIKKTALQTMK
HHHHHHHCEEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHH
NAGVHVHVMNRPRFPFFFFHMQKRNHRKITVIDGKIGYIGGFNIAEEYLGKKAKFGNWED
CCCCEEEEECCCCCCEEEEEEECCCCCEEEEEECCEEEECCCHHHHHHCCCCCCCCCCCC
YHLRMIGEGVHDLQTLFASDLKRNTGIELGSDVWPKLQQGTISHKIYATDGYSLENIYLA
EEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCEEEEEECCCCCCCCEEEH
NIAQAKNRLTVCTPYYIPSKPLQEALINARKNGVSVRIIVPMKSDHPLVREAAFTYYSEL
HHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
LDAGCLIYRYYQGFYHVKALIIDDHLSIIGTANFDKRSLFLNEEVNVEIDDEAFTSEVYA
HHHHHHHHHHHHHHEEEEEEEEECCEEEEEECCCCCCEEEECCCCCEEECCHHHHHHHHH
TIEEDMKKSELLTMEDFSKRTFRQRPAEWLGRALSYFL
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCH
>Mature Secondary Structure
MKVFIVIMIIVVIFFALILLDIFMGRAGYRKKAYEPVFSKKKSDIELIHCGADLVERMMN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCCCCEEEEECCHHHHHHHHH
DIRQAASSVHMMFFIMKNDEVSHNMYTLLKTKAQAGVSVYLLLDWAGCRAIKKTALQTMK
HHHHHHHCEEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHH
NAGVHVHVMNRPRFPFFFFHMQKRNHRKITVIDGKIGYIGGFNIAEEYLGKKAKFGNWED
CCCCEEEEECCCCCCEEEEEEECCCCCEEEEEECCEEEECCCHHHHHHCCCCCCCCCCCC
YHLRMIGEGVHDLQTLFASDLKRNTGIELGSDVWPKLQQGTISHKIYATDGYSLENIYLA
EEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCEEEEEECCCCCCCCEEEH
NIAQAKNRLTVCTPYYIPSKPLQEALINARKNGVSVRIIVPMKSDHPLVREAAFTYYSEL
HHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
LDAGCLIYRYYQGFYHVKALIIDDHLSIIGTANFDKRSLFLNEEVNVEIDDEAFTSEVYA
HHHHHHHHHHHHHHEEEEEEEEECCEEEEEECCCCCCEEEECCCCCEEECCHHHHHHHHH
TIEEDMKKSELLTMEDFSKRTFRQRPAEWLGRALSYFL
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377