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The map label for this gene is ywkA

Identifier: 16080758

GI number: 16080758

Start: 3800418

End: 3802166

Strand: Reverse

Name: ywkA

Synonym: BSU37050

Alternate gene names: 16080758

Gene position: 3802166-3800418 (Counterclockwise)

Preceding gene: 16080759

Following gene: 16080757

Centisome position: 90.19

GC content: 42.6

Gene sequence:

>1749_bases
ATGGGTTATTACTTAACTTGGTTAACTATTTCAAGAAAGAAGGAGATTTGCTTGAATAACATAAAAAAAACGAAAGAAGG
CCACTTAGAAACAACTCTTAGAGGAAAAGAAGTTTTATCTATTCCTACTTTAAATAAAGGCGTTGCTTTTTCACTAGAAG
AAAGACAAGAACTCGGCTTAGAAGGGCTTCTGCCGCCAACTGTACTTTCTCTTGATCAACAAGCACAACGCGCGTACGAG
CAATTCCAAGCGCAGCCTGACCGCCTTCGTCAAAATGTTTACTTAAGTGATCTTGCAAACCGTAACGAAGTTCTTTTCTA
TAAATTGCTGAAAAACCATCTTCGCGAAATGCTTCCTGTTGTGTACACGCCAACAGTTGGTGAAGCGATTCAGGAATACA
GCCATGAATATAGAAGACCTCAAGGAATCTACTTGTCAATCGATAACATTGACGGTATTGAAAAAGCGTTTGAAAACCTT
CATGCGACAGCAGGCGATATTGATCTTATCGTTGCTACTGACTCCGAAAGCATTCTCGGTATCGGTGACTGGGGTGTAGG
CGGTATTAATATTGCAATCGGTAAATTGGCTGTCTACACTGCTGCAGCTGGTATTGACCCTAGCCGTGTTATCCCGGTTG
TGCTTGATGTCGGTACGAACAACGAAAAACTTCTAAACGATCCTTTATATATTGGTAACAAGCATGAACGTGTTCAAGGT
GAACGCTACGAAGCTTTCATTGACGCTTATGTAAAAGCTGCATTGAAATTCTTCCCTAAAGCGCTTCTTCACTGGGAAGA
CCTTGGCAACAAAAATGCACGCAACATCATGAAAAAATACAATCATGAAATCCTGACATTTAATGATGATATTCAAGGTA
CTGGCGCGATTACACTTGCAGGTGTGCTTGCCGCTATGAAGAAAACCGGCGCTTCTATTAAAGATCAGCGTGTTGTTATC
TTCGGTGCTGGTTCTGCTGGTATCGGTATTGCTGACCAAATCCGTGACACAATGGTGCTAGCCGGATTATCTGAAGAAGA
AGCTAACAAACGTTTCTACACACTTGATTACAGAGGCTTGCTGACTGAAGACATCGAAGGCATCCTTGATTTCCAAAAAC
CTTACCTTCGCAATGCGGATGAAGTGAAAGACTGGAAGCGCGATGAAAAAGGTCAAATTCCATTCGATGAAGTTGTTCGT
CAAGCGAAACCAACAATCTTGATTGGTACTTCAGGGGTTTCAGGTGCATTCACAGAGGAAATTGTAAAAGAAATGGCATC
TCACGTAGACCGTCCGGTTATCATGCCGATGTCTAACCCGACTCACCTTGCTGAAGCTGTTCCTGAAGATCTGTTCAAAT
GGACTGACGGAAAAGTGCTTATTGCAACAGGAAGCCCATTTGACAATGTAGAATACAACGGTGTTTCTTATGAAATCGGA
CAATCTAACAACGCATTCGCGTTCCCTGGTCTTGGACTTGGTTCAATTGTAGCTGAAGCGCGTATCATTACACCTGCTAT
GTTTGCTGCAACTGCTGATGCGATTGCTGAAATGGTTGATCTTGAAACACCTGGAGCAGGATTGCTTCCAAGCATTGATA
AGCTTCAAGAGGTTTCTATCCAAGTTGCGATTGCCGTTGCTGAAGCAGCAATTAAAGACGGCGTGGCAAATCGTCAGCCT
GAAGATGTGAAACAAGCGGTACTTGATGCAATGTGGACACCTGAATACAAAAAAGTCATCGCGAAATAA

Upstream 100 bases:

>100_bases
TGACAGGCATACAATAGCTTACGTAATGTAAATAGTTTTGAGACATTGTTCGAATAATTGTCACAAAGTCGCATAAAGAT
CATGATCGGTCATATTTTAG

Downstream 100 bases:

>100_bases
TTAGCGAGTAAATTTTTCTGTCCGAGGGAAAGCTTTTTGTAAAGCTAAAGTATACGGACATAGGATTTGGAGGAGAATAT
TTTGAGCATCTTAGATATCT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 2

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MGYYLTWLTISRKKEICLNNIKKTKEGHLETTLRGKEVLSIPTLNKGVAFSLEERQELGLEGLLPPTVLSLDQQAQRAYE
QFQAQPDRLRQNVYLSDLANRNEVLFYKLLKNHLREMLPVVYTPTVGEAIQEYSHEYRRPQGIYLSIDNIDGIEKAFENL
HATAGDIDLIVATDSESILGIGDWGVGGINIAIGKLAVYTAAAGIDPSRVIPVVLDVGTNNEKLLNDPLYIGNKHERVQG
ERYEAFIDAYVKAALKFFPKALLHWEDLGNKNARNIMKKYNHEILTFNDDIQGTGAITLAGVLAAMKKTGASIKDQRVVI
FGAGSAGIGIADQIRDTMVLAGLSEEEANKRFYTLDYRGLLTEDIEGILDFQKPYLRNADEVKDWKRDEKGQIPFDEVVR
QAKPTILIGTSGVSGAFTEEIVKEMASHVDRPVIMPMSNPTHLAEAVPEDLFKWTDGKVLIATGSPFDNVEYNGVSYEIG
QSNNAFAFPGLGLGSIVAEARIITPAMFAATADAIAEMVDLETPGAGLLPSIDKLQEVSIQVAIAVAEAAIKDGVANRQP
EDVKQAVLDAMWTPEYKKVIAK

Sequences:

>Translated_582_residues
MGYYLTWLTISRKKEICLNNIKKTKEGHLETTLRGKEVLSIPTLNKGVAFSLEERQELGLEGLLPPTVLSLDQQAQRAYE
QFQAQPDRLRQNVYLSDLANRNEVLFYKLLKNHLREMLPVVYTPTVGEAIQEYSHEYRRPQGIYLSIDNIDGIEKAFENL
HATAGDIDLIVATDSESILGIGDWGVGGINIAIGKLAVYTAAAGIDPSRVIPVVLDVGTNNEKLLNDPLYIGNKHERVQG
ERYEAFIDAYVKAALKFFPKALLHWEDLGNKNARNIMKKYNHEILTFNDDIQGTGAITLAGVLAAMKKTGASIKDQRVVI
FGAGSAGIGIADQIRDTMVLAGLSEEEANKRFYTLDYRGLLTEDIEGILDFQKPYLRNADEVKDWKRDEKGQIPFDEVVR
QAKPTILIGTSGVSGAFTEEIVKEMASHVDRPVIMPMSNPTHLAEAVPEDLFKWTDGKVLIATGSPFDNVEYNGVSYEIG
QSNNAFAFPGLGLGSIVAEARIITPAMFAATADAIAEMVDLETPGAGLLPSIDKLQEVSIQVAIAVAEAAIKDGVANRQP
EDVKQAVLDAMWTPEYKKVIAK
>Mature_581_residues
GYYLTWLTISRKKEICLNNIKKTKEGHLETTLRGKEVLSIPTLNKGVAFSLEERQELGLEGLLPPTVLSLDQQAQRAYEQ
FQAQPDRLRQNVYLSDLANRNEVLFYKLLKNHLREMLPVVYTPTVGEAIQEYSHEYRRPQGIYLSIDNIDGIEKAFENLH
ATAGDIDLIVATDSESILGIGDWGVGGINIAIGKLAVYTAAAGIDPSRVIPVVLDVGTNNEKLLNDPLYIGNKHERVQGE
RYEAFIDAYVKAALKFFPKALLHWEDLGNKNARNIMKKYNHEILTFNDDIQGTGAITLAGVLAAMKKTGASIKDQRVVIF
GAGSAGIGIADQIRDTMVLAGLSEEEANKRFYTLDYRGLLTEDIEGILDFQKPYLRNADEVKDWKRDEKGQIPFDEVVRQ
AKPTILIGTSGVSGAFTEEIVKEMASHVDRPVIMPMSNPTHLAEAVPEDLFKWTDGKVLIATGSPFDNVEYNGVSYEIGQ
SNNAFAFPGLGLGSIVAEARIITPAMFAATADAIAEMVDLETPGAGLLPSIDKLQEVSIQVAIAVAEAAIKDGVANRQPE
DVKQAVLDAMWTPEYKKVIAK

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family

Homologues:

Organism=Homo sapiens, GI62420882, Length=548, Percent_Identity=39.2335766423358, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI62420880, Length=548, Percent_Identity=39.2335766423358, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI239049447, Length=548, Percent_Identity=39.2335766423358, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI4505145, Length=561, Percent_Identity=39.2156862745098, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI4505143, Length=557, Percent_Identity=38.0610412926391, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI270265879, Length=468, Percent_Identity=40.8119658119658, Blast_Score=353, Evalue=3e-97,
Organism=Escherichia coli, GI87081919, Length=558, Percent_Identity=43.3691756272401, Blast_Score=470, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17537199, Length=555, Percent_Identity=41.6216216216216, Blast_Score=421, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6322823, Length=581, Percent_Identity=43.8898450946644, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI281362674, Length=537, Percent_Identity=40.2234636871508, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI21356279, Length=537, Percent_Identity=40.2234636871508, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI281362672, Length=537, Percent_Identity=40.2234636871508, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24646388, Length=541, Percent_Identity=38.6321626617375, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24646386, Length=541, Percent_Identity=38.6321626617375, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281363505, Length=577, Percent_Identity=33.4488734835355, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI78707236, Length=577, Percent_Identity=33.4488734835355, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI281363503, Length=539, Percent_Identity=34.6938775510204, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78707232, Length=512, Percent_Identity=35.9375, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78707242, Length=574, Percent_Identity=35.3658536585366, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI78707238, Length=574, Percent_Identity=35.3658536585366, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI78707240, Length=544, Percent_Identity=35.8455882352941, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI19922384, Length=598, Percent_Identity=27.5919732441472, Blast_Score=241, Evalue=7e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MAO2_BACSU (P45868)

Other databases:

- EMBL:   Z49782
- EMBL:   AL009126
- PIR:   S55433
- RefSeq:   NP_391586.1
- ProteinModelPortal:   P45868
- SMR:   P45868
- EnsemblBacteria:   EBBACT00000003481
- GeneID:   937045
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37050
- NMPDR:   fig|224308.1.peg.3712
- GenoList:   BSU37050
- GeneTree:   EBGT00050000001535
- HOGENOM:   HBG289821
- OMA:   NARNIMK
- PhylomeDB:   P45868
- ProtClustDB:   PRK13529
- BioCyc:   BSUB:BSU37050-MONOMER
- BRENDA:   1.1.1.38
- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000106
- PRINTS:   PR00072
- SMART:   SM00919

Pfam domain/function: PF00390 malic; PF03949 Malic_M

EC number: =1.1.1.38

Molecular weight: Translated: 64104; Mature: 63973

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: ACT_SITE 122-122 ACT_SITE 195-195 BINDING 290-290 BINDING 439-439

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYYLTWLTISRKKEICLNNIKKTKEGHLETTLRGKEVLSIPTLNKGVAFSLEERQELGL
CCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEECCCEEEECCCCCCCCEEEEHHHHHCCC
EGLLPPTVLSLDQQAQRAYEQFQAQPDRLRQNVYLSDLANRNEVLFYKLLKNHLREMLPV
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCE
VYTPTVGEAIQEYSHEYRRPQGIYLSIDNIDGIEKAFENLHATAGDIDLIVATDSESILG
EECCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEE
IGDWGVGGINIAIGKLAVYTAAAGIDPSRVIPVVLDVGTNNEKLLNDPLYIGNKHERVQG
ECCCCCCCEEEEHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEECCCEEECCCHHHCCC
ERYEAFIDAYVKAALKFFPKALLHWEDLGNKNARNIMKKYNHEILTFNDDIQGTGAITLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHH
GVLAAMKKTGASIKDQRVVIFGAGSAGIGIADQIRDTMVLAGLSEEEANKRFYTLDYRGL
HHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHEECCCHHHCCCEEEEEECCCC
LTEDIEGILDFQKPYLRNADEVKDWKRDEKGQIPFDEVVRQAKPTILIGTSGVSGAFTEE
HHHHHHHHHHHCCHHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCEEEEECCCCCCHHHHH
IVKEMASHVDRPVIMPMSNPTHLAEAVPEDLFKWTDGKVLIATGSPFDNVEYNGVSYEIG
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECCEEEEEC
QSNNAFAFPGLGLGSIVAEARIITPAMFAATADAIAEMVDLETPGAGLLPSIDKLQEVSI
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
QVAIAVAEAAIKDGVANRQPEDVKQAVLDAMWTPEYKKVIAK
HEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
GYYLTWLTISRKKEICLNNIKKTKEGHLETTLRGKEVLSIPTLNKGVAFSLEERQELGL
CEEEEEEEECCCHHHHHHHHHHCCCCCCCEEECCCEEEECCCCCCCCEEEEHHHHHCCC
EGLLPPTVLSLDQQAQRAYEQFQAQPDRLRQNVYLSDLANRNEVLFYKLLKNHLREMLPV
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCE
VYTPTVGEAIQEYSHEYRRPQGIYLSIDNIDGIEKAFENLHATAGDIDLIVATDSESILG
EECCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEE
IGDWGVGGINIAIGKLAVYTAAAGIDPSRVIPVVLDVGTNNEKLLNDPLYIGNKHERVQG
ECCCCCCCEEEEHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEECCCEEECCCHHHCCC
ERYEAFIDAYVKAALKFFPKALLHWEDLGNKNARNIMKKYNHEILTFNDDIQGTGAITLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHH
GVLAAMKKTGASIKDQRVVIFGAGSAGIGIADQIRDTMVLAGLSEEEANKRFYTLDYRGL
HHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHEECCCHHHCCCEEEEEECCCC
LTEDIEGILDFQKPYLRNADEVKDWKRDEKGQIPFDEVVRQAKPTILIGTSGVSGAFTEE
HHHHHHHHHHHCCHHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCEEEEECCCCCCHHHHH
IVKEMASHVDRPVIMPMSNPTHLAEAVPEDLFKWTDGKVLIATGSPFDNVEYNGVSYEIG
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECCEEEEEC
QSNNAFAFPGLGLGSIVAEARIITPAMFAATADAIAEMVDLETPGAGLLPSIDKLQEVSI
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
QVAIAVAEAAIKDGVANRQPEDVKQAVLDAMWTPEYKKVIAK
HEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 12949160