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The map label for this gene is ywlF

Identifier: 16080745

GI number: 16080745

Start: 3791199

End: 3791648

Strand: Reverse

Name: ywlF

Synonym: BSU36920

Alternate gene names: 16080745

Gene position: 3791648-3791199 (Counterclockwise)

Preceding gene: 16080746

Following gene: 16080744

Centisome position: 89.94

GC content: 48.22

Gene sequence:

>450_bases
ATGAAAGTAGCCATTGCATCGGATCATGGCGGCGTTCACATTCGAAATGAAATCAAAGAGTTAATGGACGAATTGCAAAT
TGAATATATTGATATGGGCTGTGACTGCGGCAGCGGCTCTGTCGATTATCCGGATTATGCTTTTCCGGTGGCCGAAAAAG
TGGTTAGCGGCGAAGTTGACAGAGGCATTTTAATTTGCGGGACAGGCATCGGCATGAGCATTTCCGCTAATAAAGTAAAA
GGGATTCGCTGCGCGCTGGCGCACGATACCTTCAGCGCGAAGGCGACGAGGGAGCATAATGACACAAACATCCTTGCGAT
GGGTGAACGGGTGATCGGACCTGGTTTGGCTCGGGAAATCGCAAAAATCTGGCTGACTACTGAGTTTACCGGGGGAAGAC
ACCAAACGCGTATTGGAAAAATCTCCGATTATGAAGAGAAAAACCTGTAG

Upstream 100 bases:

>100_bases
ATAGTGAAAAGCACATCATATGGTGTGAATCAAAGAGCGGACAGGCTGCGCGGTCAATAAAGCGGGCGGGCCGCATCACA
CACGCTGGGAGGAAAAGCAG

Downstream 100 bases:

>100_bases
GGGTGCTGTTTAATGAATGAGTTGAAACAAACGTGGAAGACGATGCTGTCTGAGTTTCAGGACCAAGCGGAATTGAAACA
AGATCAGCTCTTCGTCCTTG

Product: ribose-5-phosphate isomerase B

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKVAIASDHGGVHIRNEIKELMDELQIEYIDMGCDCGSGSVDYPDYAFPVAEKVVSGEVDRGILICGTGIGMSISANKVK
GIRCALAHDTFSAKATREHNDTNILAMGERVIGPGLAREIAKIWLTTEFTGGRHQTRIGKISDYEEKNL

Sequences:

>Translated_149_residues
MKVAIASDHGGVHIRNEIKELMDELQIEYIDMGCDCGSGSVDYPDYAFPVAEKVVSGEVDRGILICGTGIGMSISANKVK
GIRCALAHDTFSAKATREHNDTNILAMGERVIGPGLAREIAKIWLTTEFTGGRHQTRIGKISDYEEKNL
>Mature_149_residues
MKVAIASDHGGVHIRNEIKELMDELQIEYIDMGCDCGSGSVDYPDYAFPVAEKVVSGEVDRGILICGTGIGMSISANKVK
GIRCALAHDTFSAKATREHNDTNILAMGERVIGPGLAREIAKIWLTTEFTGGRHQTRIGKISDYEEKNL

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family

Homologues:

Organism=Escherichia coli, GI1790528, Length=146, Percent_Identity=47.2602739726027, Blast_Score=134, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YWLF_BACSU (P39156)

Other databases:

- EMBL:   Z38002
- EMBL:   AL009126
- PIR:   I40481
- RefSeq:   NP_391573.1
- ProteinModelPortal:   P39156
- SMR:   P39156
- EnsemblBacteria:   EBBACT00000002937
- GeneID:   937014
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU36920
- NMPDR:   fig|224308.1.peg.3699
- GenoList:   BSU36920
- GeneTree:   EBGT00050000002850
- HOGENOM:   HBG636953
- OMA:   YEGGRHE
- PhylomeDB:   P39156
- ProtClustDB:   PRK05571
- BioCyc:   BSUB:BSU36920-MONOMER
- InterPro:   IPR003500
- InterPro:   IPR004785
- Gene3D:   G3DSA:3.40.1400.10
- PIRSF:   PIRSF005384
- TIGRFAMs:   TIGR01120
- TIGRFAMs:   TIGR00689

Pfam domain/function: PF02502 LacAB_rpiB; SSF89623 Rib/Gal_isomrase

EC number: 5.3.1.6

Molecular weight: Translated: 16211; Mature: 16211

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAIASDHGGVHIRNEIKELMDELQIEYIDMGCDCGSGSVDYPDYAFPVAEKVVSGEVD
CEEEEECCCCCEEEHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCC
RGILICGTGIGMSISANKVKGIRCALAHDTFSAKATREHNDTNILAMGERVIGPGLAREI
CCEEEEECCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHH
AKIWLTTEFTGGRHQTRIGKISDYEEKNL
HHHHEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVAIASDHGGVHIRNEIKELMDELQIEYIDMGCDCGSGSVDYPDYAFPVAEKVVSGEVD
CEEEEECCCCCEEEHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCC
RGILICGTGIGMSISANKVKGIRCALAHDTFSAKATREHNDTNILAMGERVIGPGLAREI
CCEEEEECCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHH
AKIWLTTEFTGGRHQTRIGKISDYEEKNL
HHHHEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377