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The map label for this gene is clsA

Identifier: 16080712

GI number: 16080712

Start: 3762664

End: 3764112

Strand: Direct

Name: clsA

Synonym: BSU36590

Alternate gene names: 16080712

Gene position: 3762664-3764112 (Clockwise)

Preceding gene: 16080710

Following gene: 16080714

Centisome position: 89.26

GC content: 43.06

Gene sequence:

>1449_bases
GTGAGTATTTCTTCCATCCTTTTATCACTTTTTTTCATTTTAAATATTCTTTTGGCCATCATTGTCATCTTCAAAGAACG
GCGCGATGCCAGTGCATCTTGGGCGTGGCTGCTTGTTCTTTTCTTTATTCCCGTTTTAGGCTTTATTTTATACTTATTAT
TCGGCCATAATCTCAGACGCAAGCATTTGTTTCAATGGGAAGACCGGAAAAAGATTGGAATTGAAAGGCTGCTGAAGCAT
CAGCTTGAAGATTTAGAAACCAAGCAATTTCAGTTTAACAACCGGGCAACCTTTGATAACAAAGATTTAATTTATATGCT
GATTATGAATAACCATGCTGTGTTTACAGAGGATAATTCAGTTGACGTCATTACAGATGGACGGGATAAATTTCAGCGGC
TGCTGAGTGACATTTCAAAGGCTAAGGATCATATCCATTTACAGTATTATATTTATAAAGGCGATGAATTAGGCAAAAAA
TTGCGGGACGCTCTCATCCAAAAGGCGAAAGAAGGTGTTCAGGTCAGAGTGCTTTATGATGAACTCGGGTCCAGAACGCT
GAGAAAAAAGTTTTTTAAAGAGCTTCGCGAGGCTGGCGGGCATGTGGAGGTCTTTTTCCCGTCAAAGCTCAGGCCGATTA
ACCTGCGGCTGAATTACCGGAACCATCGAAAGCTTGTGATCATAGACGGTATGACCGGTTATGTCGGAGGCTTTAACGTC
GGAGATGAGTATCTAGGGCTTAATCCGAAATTCGGTTATTGGCGGGATACGCATATCCGGCTGCAAGGAACGGCCGTGCA
CGCCATCCAAACACGATTCATCCTTGACTGGAACCAAGCTTCTCATCACCATACGCTGACATATATTCCAAATCACTTTC
CTGATTACGGCCCAAAAGGCAACGTCGGGATGCAGATTGTCACAAGCGGCCCTGATTCGGAATGGGAACAAATTAAAAAT
GGCTACATCAAAATGATTTCGAACGCCAAGCGTTCCATTTTGATACAAACACCTTATTTTATCCCAGATGCAAGCCTGCT
GGATGCTTTAAGGATCGCTTGCCTTTCGGGCATTGACGTTAACATTATGATTCCGAACAAGCCCGATCACGCCTTTGTAT
ACTGGGCGACACTTTCGTATATCGGCGATCTGCTGAAAGCCGGAGCCACTGTCTATATTTACGATAACGGCTTTATTCAC
GCGAAAACCATCGTGGTGGATGACGAAATCGCTTCAGTCGGCACAGCGAACATTGATGTCCGAAGCTTCAGGCTGAATTT
TGAGGTTAATGCATTTATTTACGATATCACCATTGCTAAAAAACTGGTTTCAACTTTTAAAGAAGATTTGCTAGTATCCA
GAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTTGTGGATTCGAATAAAAGAATCCGTCTCGCGGCTGTTGTCGCCG
ATCTTATAA

Upstream 100 bases:

>100_bases
AAATGTGAGTGTTATAATTTGTAATGGGAATCAGATCACTGTTATTCATGGAATACTAATGGCAATCTTCTTGATAAATA
TGAATGAGCGGGGTTACAAA

Downstream 100 bases:

>100_bases
GATGCGTAAACCCCCGGCCTTTACGGCCGGGGGTTTTCCTGATGGTCACAGTAAATGATGATTGCCTCCCGCAAAAAAGC
GGCAAGACCTTCTCCATATT

Product: cardiolipin synthase

Products: NA

Alternate protein names: CL synthase 2

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSISSILLSLFFILNILLAIIVIFKERRDASASWAWLLVLFFIPVLGFILYLLFGHNLRRKHLFQWEDRKKIGIERLLKH
QLEDLETKQFQFNNRATFDNKDLIYMLIMNNHAVFTEDNSVDVITDGRDKFQRLLSDISKAKDHIHLQYYIYKGDELGKK
LRDALIQKAKEGVQVRVLYDELGSRTLRKKFFKELREAGGHVEVFFPSKLRPINLRLNYRNHRKLVIIDGMTGYVGGFNV
GDEYLGLNPKFGYWRDTHIRLQGTAVHAIQTRFILDWNQASHHHTLTYIPNHFPDYGPKGNVGMQIVTSGPDSEWEQIKN
GYIKMISNAKRSILIQTPYFIPDASLLDALRIACLSGIDVNIMIPNKPDHAFVYWATLSYIGDLLKAGATVYIYDNGFIH
AKTIVVDDEIASVGTANIDVRSFRLNFEVNAFIYDITIAKKLVSTFKEDLLVSRKFTYEEYLQRPLWIRIKESVSRLLSP
IL

Sequences:

>Translated_482_residues
MSISSILLSLFFILNILLAIIVIFKERRDASASWAWLLVLFFIPVLGFILYLLFGHNLRRKHLFQWEDRKKIGIERLLKH
QLEDLETKQFQFNNRATFDNKDLIYMLIMNNHAVFTEDNSVDVITDGRDKFQRLLSDISKAKDHIHLQYYIYKGDELGKK
LRDALIQKAKEGVQVRVLYDELGSRTLRKKFFKELREAGGHVEVFFPSKLRPINLRLNYRNHRKLVIIDGMTGYVGGFNV
GDEYLGLNPKFGYWRDTHIRLQGTAVHAIQTRFILDWNQASHHHTLTYIPNHFPDYGPKGNVGMQIVTSGPDSEWEQIKN
GYIKMISNAKRSILIQTPYFIPDASLLDALRIACLSGIDVNIMIPNKPDHAFVYWATLSYIGDLLKAGATVYIYDNGFIH
AKTIVVDDEIASVGTANIDVRSFRLNFEVNAFIYDITIAKKLVSTFKEDLLVSRKFTYEEYLQRPLWIRIKESVSRLLSP
IL
>Mature_481_residues
SISSILLSLFFILNILLAIIVIFKERRDASASWAWLLVLFFIPVLGFILYLLFGHNLRRKHLFQWEDRKKIGIERLLKHQ
LEDLETKQFQFNNRATFDNKDLIYMLIMNNHAVFTEDNSVDVITDGRDKFQRLLSDISKAKDHIHLQYYIYKGDELGKKL
RDALIQKAKEGVQVRVLYDELGSRTLRKKFFKELREAGGHVEVFFPSKLRPINLRLNYRNHRKLVIIDGMTGYVGGFNVG
DEYLGLNPKFGYWRDTHIRLQGTAVHAIQTRFILDWNQASHHHTLTYIPNHFPDYGPKGNVGMQIVTSGPDSEWEQIKNG
YIKMISNAKRSILIQTPYFIPDASLLDALRIACLSGIDVNIMIPNKPDHAFVYWATLSYIGDLLKAGATVYIYDNGFIHA
KTIVVDDEIASVGTANIDVRSFRLNFEVNAFIYDITIAKKLVSTFKEDLLVSRKFTYEEYLQRPLWIRIKESVSRLLSPI
L

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1787502, Length=491, Percent_Identity=28.3095723014257, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787007, Length=365, Percent_Identity=28.4931506849315, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081826, Length=397, Percent_Identity=25.1889168765743, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLS2_BACSU (P71040)

Other databases:

- EMBL:   Y08559
- EMBL:   AL009126
- PIR:   G70063
- RefSeq:   NP_391540.1
- ProteinModelPortal:   P71040
- EnsemblBacteria:   EBBACT00000001257
- GeneID:   936957
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU36590
- NMPDR:   fig|224308.1.peg.3666
- GenoList:   BSU36590
- GeneTree:   EBGT00070000032016
- HOGENOM:   HBG694312
- OMA:   MANCVEL
- PhylomeDB:   P71040
- ProtClustDB:   CLSK888033
- BioCyc:   BSUB:BSU36590-MONOMER
- HAMAP:   MF_00190
- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736
- PANTHER:   PTHR18896
- SMART:   SM00155

Pfam domain/function: PF00614 PLDc

EC number: 2.7.8.-

Molecular weight: Translated: 55867; Mature: 55735

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50035 PLD

Important sites: ACT_SITE 222-222 ACT_SITE 224-224 ACT_SITE 229-229 ACT_SITE 400-400 ACT_SITE 402-402 ACT_SITE 407-407

Signals:

None

Transmembrane regions:

HASH(0x12cfcdec)-; HASH(0x119e432c)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISSILLSLFFILNILLAIIVIFKERRDASASWAWLLVLFFIPVLGFILYLLFGHNLRR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
KHLFQWEDRKKIGIERLLKHQLEDLETKQFQFNNRATFDNKDLIYMLIMNNHAVFTEDNS
HHCCCCCCHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCEEEEEEECCEEEEECCCC
VDVITDGRDKFQRLLSDISKAKDHIHLQYYIYKGDELGKKLRDALIQKAKEGVQVRVLYD
EEEEECCHHHHHHHHHHHHHCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEHH
ELGSRTLRKKFFKELREAGGHVEVFFPSKLRPINLRLNYRNHRKLVIIDGMTGYVGGFNV
HHCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCC
GDEYLGLNPKFGYWRDTHIRLQGTAVHAIQTRFILDWNQASHHHTLTYIPNHFPDYGPKG
CCHHCCCCCCCCCEECCEEEEECEEEEEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCC
NVGMQIVTSGPDSEWEQIKNGYIKMISNAKRSILIQTPYFIPDASLLDALRIACLSGIDV
CCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEE
NIMIPNKPDHAFVYWATLSYIGDLLKAGATVYIYDNGFIHAKTIVVDDEIASVGTANIDV
EEEECCCCCCEEEEEHHHHHHHHHHHCCCEEEEEECCEEEEEEEEEECHHHHCCCCCEEE
RSFRLNFEVNAFIYDITIAKKLVSTFKEDLLVSRKFTYEEYLQRPLWIRIKESVSRLLSP
EEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEHHHHHHHHHC
IL
CH
>Mature Secondary Structure 
SISSILLSLFFILNILLAIIVIFKERRDASASWAWLLVLFFIPVLGFILYLLFGHNLRR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
KHLFQWEDRKKIGIERLLKHQLEDLETKQFQFNNRATFDNKDLIYMLIMNNHAVFTEDNS
HHCCCCCCHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCEEEEEEECCEEEEECCCC
VDVITDGRDKFQRLLSDISKAKDHIHLQYYIYKGDELGKKLRDALIQKAKEGVQVRVLYD
EEEEECCHHHHHHHHHHHHHCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEHH
ELGSRTLRKKFFKELREAGGHVEVFFPSKLRPINLRLNYRNHRKLVIIDGMTGYVGGFNV
HHCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCC
GDEYLGLNPKFGYWRDTHIRLQGTAVHAIQTRFILDWNQASHHHTLTYIPNHFPDYGPKG
CCHHCCCCCCCCCEECCEEEEECEEEEEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCC
NVGMQIVTSGPDSEWEQIKNGYIKMISNAKRSILIQTPYFIPDASLLDALRIACLSGIDV
CCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEE
NIMIPNKPDHAFVYWATLSYIGDLLKAGATVYIYDNGFIHAKTIVVDDEIASVGTANIDV
EEEECCCCCCEEEEEHHHHHHHHHHHCCCEEEEEECCEEEEEEEEEECHHHHCCCCCEEE
RSFRLNFEVNAFIYDITIAKKLVSTFKEDLLVSRKFTYEEYLQRPLWIRIKESVSRLLSP
EEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEHHHHHHHHHC
IL
CH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377