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The map label for this gene is rbsK

Identifier: 16080645

GI number: 16080645

Start: 3702393

End: 3703274

Strand: Direct

Name: rbsK

Synonym: BSU35920

Alternate gene names: 16080645

Gene position: 3702393-3703274 (Clockwise)

Preceding gene: 16080644

Following gene: 16080646

Centisome position: 87.83

GC content: 50.91

Gene sequence:

>882_bases
ATGCGTAATATTTGTGTGATTGGTAGCTGTTCTATGGATTTAGTGGTCACCTCGGACAAACGCCCAAAAGCCGGTGAAAC
GGTTCTTGGCACGTCATTTCAGACTGTGCCGGGCGGCAAAGGAGCAAATCAGGCCGTTGCCGCAGCCAGACTTGGGGCGC
AAGTGTTTATGGTCGGCAAAGTTGGAGACGATCATTATGGAACAGCTATTTTGAATAATCTCAAAGCTAACGGCGTTCGC
ACTGATTATATGGAACCGGTTACACATACGGAAAGCGGAACCGCTCATATTGTGCTTGCTGAAGGCGACAACAGCATTGT
CGTTGTCAAAGGCGCGAACGATGACATCACCCCAGCGTATGCGTTAAACGCGCTCGAACAGATTGAAAAGGTCGATATGG
TGCTGATTCAGCAGGAAATCCCAGAAGAAACGGTTGATGAGGTGTGCAAATACTGCCATTCTCACGATATCCCGATCATA
CTAAACCCAGCACCGGCCCGCCCGCTCAAGCAGGAAACAATTGATCACGCTACCTATCTGACGCCGAATGAACACGAGGC
TTCGATTTTATTCCCTGAGCTTACCATTTCCGAAGCTCTGGCGCTTTATCCGGCAAAGCTCTTTATTACGGAAGGAAAAC
AAGGCGTCCGCTATTCGGCTGGCAGCAAAGAGGTGCTCATCCCGTCCTTCCCTGTAGAACCCGTTGATACAACCGGAGCC
GGTGATACGTTTAACGCAGCATTTGCCGTGGCCCTGGCTGAGGGAAAAGACATTGAAGCCGCATTGCGGTTTGCCAATCG
TGCCGCTTCCCTTTCTGTCTGCTCCTTCGGTGCCCAAGGCGGAATGCCGACAAGAAATGAAGTAGAGGAGCTGCTGTCAT
GA

Upstream 100 bases:

>100_bases
CTGCTTTTGGGTATCATTAAAAAACAGCCGCTGGCAGAAACGGCAATTCAAATGCCTGTCACCTATATAGGAAGAAAGAC
GACAAGAAAGGAAGATTAAG

Downstream 100 bases:

>100_bases
AAAAACACGGTATACTGAACAGCCATCTTGCCAAGATTTTAGCCGACCTTGGCCACACTGATAAAATTGTCATCGCGGAT
GCCGGACTGCCGGTTCCTGA

Product: ribokinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MRNICVIGSCSMDLVVTSDKRPKAGETVLGTSFQTVPGGKGANQAVAAARLGAQVFMVGKVGDDHYGTAILNNLKANGVR
TDYMEPVTHTESGTAHIVLAEGDNSIVVVKGANDDITPAYALNALEQIEKVDMVLIQQEIPEETVDEVCKYCHSHDIPII
LNPAPARPLKQETIDHATYLTPNEHEASILFPELTISEALALYPAKLFITEGKQGVRYSAGSKEVLIPSFPVEPVDTTGA
GDTFNAAFAVALAEGKDIEAALRFANRAASLSVCSFGAQGGMPTRNEVEELLS

Sequences:

>Translated_293_residues
MRNICVIGSCSMDLVVTSDKRPKAGETVLGTSFQTVPGGKGANQAVAAARLGAQVFMVGKVGDDHYGTAILNNLKANGVR
TDYMEPVTHTESGTAHIVLAEGDNSIVVVKGANDDITPAYALNALEQIEKVDMVLIQQEIPEETVDEVCKYCHSHDIPII
LNPAPARPLKQETIDHATYLTPNEHEASILFPELTISEALALYPAKLFITEGKQGVRYSAGSKEVLIPSFPVEPVDTTGA
GDTFNAAFAVALAEGKDIEAALRFANRAASLSVCSFGAQGGMPTRNEVEELLS
>Mature_293_residues
MRNICVIGSCSMDLVVTSDKRPKAGETVLGTSFQTVPGGKGANQAVAAARLGAQVFMVGKVGDDHYGTAILNNLKANGVR
TDYMEPVTHTESGTAHIVLAEGDNSIVVVKGANDDITPAYALNALEQIEKVDMVLIQQEIPEETVDEVCKYCHSHDIPII
LNPAPARPLKQETIDHATYLTPNEHEASILFPELTISEALALYPAKLFITEGKQGVRYSAGSKEVLIPSFPVEPVDTTGA
GDTFNAAFAVALAEGKDIEAALRFANRAASLSVCSFGAQGGMPTRNEVEELLS

Specific function: Ribose metabolism; first step. [C]

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family

Homologues:

Organism=Homo sapiens, GI11545855, Length=296, Percent_Identity=35.1351351351351, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI1790193, Length=300, Percent_Identity=36, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI87082343, Length=297, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081971, Length=281, Percent_Identity=26.6903914590747, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788491, Length=289, Percent_Identity=24.9134948096886, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17532905, Length=302, Percent_Identity=36.4238410596026, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6319884, Length=333, Percent_Identity=30.6306306306306, Blast_Score=119, Evalue=7e-28,
Organism=Drosophila melanogaster, GI20128807, Length=300, Percent_Identity=33, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24583886, Length=308, Percent_Identity=28.2467532467532, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RBSK_BACSU (P36945)

Other databases:

- EMBL:   Z25798
- EMBL:   Z92953
- EMBL:   AL009126
- PIR:   D69690
- RefSeq:   NP_391473.1
- ProteinModelPortal:   P36945
- SMR:   P36945
- EnsemblBacteria:   EBBACT00000000963
- GeneID:   936844
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU35920
- NMPDR:   fig|224308.1.peg.3599
- GenoList:   BSU35920
- GeneTree:   EBGT00050000001879
- HOGENOM:   HBG697973
- OMA:   NEHECKE
- PhylomeDB:   P36945
- ProtClustDB:   CLSK887989
- BioCyc:   BSUB:BSU35920-MONOMER
- BRENDA:   2.7.1.15
- GO:   GO:0005737
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR011877
- InterPro:   IPR002139
- PRINTS:   PR00990
- TIGRFAMs:   TIGR02152

Pfam domain/function: PF00294 PfkB

EC number: =2.7.1.15

Molecular weight: Translated: 31139; Mature: 31139

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNICVIGSCSMDLVVTSDKRPKAGETVLGTSFQTVPGGKGANQAVAAARLGAQVFMVGK
CCCEEEEECCCEEEEEECCCCCCCCCEEECCCEEECCCCCCCCHHHHHHHCCCEEEEEEE
VGDDHYGTAILNNLKANGVRTDYMEPVTHTESGTAHIVLAEGDNSIVVVKGANDDITPAY
CCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCHHH
ALNALEQIEKVDMVLIQQEIPEETVDEVCKYCHSHDIPIILNPAPARPLKQETIDHATYL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHEEC
TPNEHEASILFPELTISEALALYPAKLFITEGKQGVRYSAGSKEVLIPSFPVEPVDTTGA
CCCCCCCEEEECCHHHHHHHHHCCEEEEEECCCCCCEECCCCCEEEECCCCCCCCCCCCC
GDTFNAAFAVALAEGKDIEAALRFANRAASLSVCSFGAQGGMPTRNEVEELLS
CCCCCCEEEEEEECCCCHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHC
>Mature Secondary Structure
MRNICVIGSCSMDLVVTSDKRPKAGETVLGTSFQTVPGGKGANQAVAAARLGAQVFMVGK
CCCEEEEECCCEEEEEECCCCCCCCCEEECCCEEECCCCCCCCHHHHHHHCCCEEEEEEE
VGDDHYGTAILNNLKANGVRTDYMEPVTHTESGTAHIVLAEGDNSIVVVKGANDDITPAY
CCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCHHH
ALNALEQIEKVDMVLIQQEIPEETVDEVCKYCHSHDIPIILNPAPARPLKQETIDHATYL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHEEC
TPNEHEASILFPELTISEALALYPAKLFITEGKQGVRYSAGSKEVLIPSFPVEPVDTTGA
CCCCCCCEEEECCHHHHHHHHHCCEEEEEECCCCCCEECCCCCEEEECCCCCCCCCCCCC
GDTFNAAFAVALAEGKDIEAALRFANRAASLSVCSFGAQGGMPTRNEVEELLS
CCCCCCEEEEEEECCCCHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7921236; 9353933; 9384377