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The map label for this gene is yvkA

Identifier: 16080574

GI number: 16080574

Start: 3618964

End: 3620298

Strand: Direct

Name: yvkA

Synonym: BSU35210

Alternate gene names: 16080574

Gene position: 3618964-3620298 (Clockwise)

Preceding gene: 16080573

Following gene: 16080582

Centisome position: 85.85

GC content: 45.84

Gene sequence:

>1335_bases
ATGAGCTCAAGAAAAGAATGGGCGCTGATCGTCTCGCTATTATTGGGGGCCATTCTGGTTCCCATTAACTCAACGATGAT
CGCGGTCGCGCTCTCATCTATTTCCCATACGTATAACGAGTCCATCGCAAGCATCACTTGGGTGGTTACCGTTTATTTAA
TCGTGATGGCAGTCACACAGCCGATTGCAGGAAAGCTCGGTGACATGTACGGCAATAAAACAATGTACTTATGGGGAGTC
GGCCTTTTTCTAATCGCATCTCTAGGCTGCGCCCTTTCACCGAGTCTGCTCCTGTTAATTGTCTTTCGGGCATTGCAGGC
TGTCGGCGGGGCATTGCTTACGCCAAACAGCATTGCCATTATCCGGCATGTTGTTTCTGAAAAACGCCTACCGAAAGTGT
TTGGATTTTTCGGACTGGGCGCGGGACTTGGTGCAGCACTCGGGCCTTTTATCGGCTCTATACTGATTGACAGCTTCAGC
TGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATTAACGATGTTTCCTCAATATAA
AGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGA
CGAAAAATGAGGCTCCATGGGGATATACTGTATACAGTGTATTGATACTTCTTTTTGTTCCGCTTTTTTTCAGAAGAGAA
AAACGTACCCAGCACCCCATCATTGACTTTGCACTGTTTAAAAGCTCAACCTTTACAAATGCGAATCTATCAGTCTTGTT
AAGCAATTTGATGATGTATGCCGTCTTATTGATTATGCCGTTATTTATGACGAACCAATTTGGGCTTAATACATCTAACA
GCGGGATGGCGCTTTCTGTATTTTCGATCTTCATGTCAGCCAGCAACTGGGTGGGCGCGCAGCTCCATCACAAATGGGGA
GCCAAAAAAATCATTTTTCTTTCATTTGCCATGATGGCTGGTGCGAATCTATTATTTTTGCTGTTAAGCAGCTCGCATTC
TGTCTTGTTCCTCATGCTCTCTCTTATCCTTGGCGGGCTCGCTTCCGGAGTCGGTCTGACCAGCATGCAGGTTTCATCCC
TTGCCACAGTAGACCCCGGTATGTCAGGAGTGGCATCTGGGATTTTCTCGACCTTCCGTTATTTCGGAAGCATCATTTCA
TCAGCCTTAATTGGCCTGATTTCGGGATATCACACCCTATTTATGATTCTCTTTGCTGTATCCATCATCGGCGTTTTTGT
TTCATTGGGCATCAAATCTGATGAAACGGCACGGATAGAAAAAAACTCAGCGTAA

Upstream 100 bases:

>100_bases
ATCAATGCCCCAGGAAAAAATGCTGGAGCATAGCACAGCTCTATTTGTCAAAGGAATTTCTGCTGATTAACTCCTTGGCA
TAATAAAAAGGAGGATCCCA

Downstream 100 bases:

>100_bases
CACAAAACAGCCCGGCATCGCATCATCCTGCGGTGCCGGGCTGCCTTTTATGATCCCGAAGCGACTGCTTCGTCTTCACG
GTCATCCTTTACGAAGAGGA

Product: efflux transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSSRKEWALIVSLLLGAILVPINSTMIAVALSSISHTYNESIASITWVVTVYLIVMAVTQPIAGKLGDMYGNKTMYLWGV
GLFLIASLGCALSPSLLLLIVFRALQAVGGALLTPNSIAIIRHVVSEKRLPKVFGFFGLGAGLGAALGPFIGSILIDSFS
WHSIFWVNIPFLAIALFTALTMFPQYKENKSDAPLDIIGSLLLAGSIVSIILLTKNEAPWGYTVYSVLILLFVPLFFRRE
KRTQHPIIDFALFKSSTFTNANLSVLLSNLMMYAVLLIMPLFMTNQFGLNTSNSGMALSVFSIFMSASNWVGAQLHHKWG
AKKIIFLSFAMMAGANLLFLLLSSSHSVLFLMLSLILGGLASGVGLTSMQVSSLATVDPGMSGVASGIFSTFRYFGSIIS
SALIGLISGYHTLFMILFAVSIIGVFVSLGIKSDETARIEKNSA

Sequences:

>Translated_444_residues
MSSRKEWALIVSLLLGAILVPINSTMIAVALSSISHTYNESIASITWVVTVYLIVMAVTQPIAGKLGDMYGNKTMYLWGV
GLFLIASLGCALSPSLLLLIVFRALQAVGGALLTPNSIAIIRHVVSEKRLPKVFGFFGLGAGLGAALGPFIGSILIDSFS
WHSIFWVNIPFLAIALFTALTMFPQYKENKSDAPLDIIGSLLLAGSIVSIILLTKNEAPWGYTVYSVLILLFVPLFFRRE
KRTQHPIIDFALFKSSTFTNANLSVLLSNLMMYAVLLIMPLFMTNQFGLNTSNSGMALSVFSIFMSASNWVGAQLHHKWG
AKKIIFLSFAMMAGANLLFLLLSSSHSVLFLMLSLILGGLASGVGLTSMQVSSLATVDPGMSGVASGIFSTFRYFGSIIS
SALIGLISGYHTLFMILFAVSIIGVFVSLGIKSDETARIEKNSA
>Mature_443_residues
SSRKEWALIVSLLLGAILVPINSTMIAVALSSISHTYNESIASITWVVTVYLIVMAVTQPIAGKLGDMYGNKTMYLWGVG
LFLIASLGCALSPSLLLLIVFRALQAVGGALLTPNSIAIIRHVVSEKRLPKVFGFFGLGAGLGAALGPFIGSILIDSFSW
HSIFWVNIPFLAIALFTALTMFPQYKENKSDAPLDIIGSLLLAGSIVSIILLTKNEAPWGYTVYSVLILLFVPLFFRREK
RTQHPIIDFALFKSSTFTNANLSVLLSNLMMYAVLLIMPLFMTNQFGLNTSNSGMALSVFSIFMSASNWVGAQLHHKWGA
KKIIFLSFAMMAGANLLFLLLSSSHSVLFLMLSLILGGLASGVGLTSMQVSSLATVDPGMSGVASGIFSTFRYFGSIISS
ALIGLISGYHTLFMILFAVSIIGVFVSLGIKSDETARIEKNSA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family

Homologues:

Organism=Escherichia coli, GI1790195, Length=373, Percent_Identity=27.6139410187668, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789042, Length=332, Percent_Identity=24.0963855421687, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87081983, Length=407, Percent_Identity=23.5872235872236, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788710, Length=301, Percent_Identity=28.2392026578073, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788392, Length=189, Percent_Identity=32.8042328042328, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790146, Length=181, Percent_Identity=30.3867403314917, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=322, Percent_Identity=22.6708074534161, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6319770, Length=376, Percent_Identity=24.468085106383, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324264, Length=170, Percent_Identity=28.8235294117647, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324954, Length=232, Percent_Identity=28.0172413793103, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YVKA_BACSU (O34502)

Other databases:

- EMBL:   AF017113
- EMBL:   AL009126
- PIR:   G70042
- RefSeq:   NP_391401.1
- ProteinModelPortal:   O34502
- EnsemblBacteria:   EBBACT00000001486
- GeneID:   938566
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU35210
- NMPDR:   fig|224308.1.peg.3527
- GenoList:   BSU35210
- GeneTree:   EBGT00050000000114
- HOGENOM:   HBG338354
- OMA:   SIASITW
- ProtClustDB:   CLSK887947
- BioCyc:   BSUB:BSU35210-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411
- PRINTS:   PR01036

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 47799; Mature: 47667

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1299c220)-; HASH(0x11b0b924)-; HASH(0x11e40658)-; HASH(0x1217019c)-; HASH(0x40c28ec4)-; HASH(0x40c16468)-; HASH(0x1287c1b0)-; HASH(0x12766834)-; HASH(0x128c964c)-; HASH(0x12a36994)-; HASH(0x125bfe54)-; HASH(0x12176928)-; HASH(0x1258b518)-; HASH(0x11c7f9f8)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRKEWALIVSLLLGAILVPINSTMIAVALSSISHTYNESIASITWVVTVYLIVMAVTQ
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIAGKLGDMYGNKTMYLWGVGLFLIASLGCALSPSLLLLIVFRALQAVGGALLTPNSIAI
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEECCCHHHH
IRHVVSEKRLPKVFGFFGLGAGLGAALGPFIGSILIDSFSWHSIFWVNIPFLAIALFTAL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
TMFPQYKENKSDAPLDIIGSLLLAGSIVSIILLTKNEAPWGYTVYSVLILLFVPLFFRRE
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
KRTQHPIIDFALFKSSTFTNANLSVLLSNLMMYAVLLIMPLFMTNQFGLNTSNSGMALSV
HCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
FSIFMSASNWVGAQLHHKWGAKKIIFLSFAMMAGANLLFLLLSSSHSVLFLMLSLILGGL
HHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHH
ASGVGLTSMQVSSLATVDPGMSGVASGIFSTFRYFGSIISSALIGLISGYHTLFMILFAV
HHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIIGVFVSLGIKSDETARIEKNSA
HHHHHHHHHCCCCCCCCCCCCCCH
>Mature Secondary Structure 
SSRKEWALIVSLLLGAILVPINSTMIAVALSSISHTYNESIASITWVVTVYLIVMAVTQ
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIAGKLGDMYGNKTMYLWGVGLFLIASLGCALSPSLLLLIVFRALQAVGGALLTPNSIAI
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEECCCHHHH
IRHVVSEKRLPKVFGFFGLGAGLGAALGPFIGSILIDSFSWHSIFWVNIPFLAIALFTAL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
TMFPQYKENKSDAPLDIIGSLLLAGSIVSIILLTKNEAPWGYTVYSVLILLFVPLFFRRE
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
KRTQHPIIDFALFKSSTFTNANLSVLLSNLMMYAVLLIMPLFMTNQFGLNTSNSGMALSV
HCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
FSIFMSASNWVGAQLHHKWGAKKIIFLSFAMMAGANLLFLLLSSSHSVLFLMLSLILGGL
HHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHH
ASGVGLTSMQVSSLATVDPGMSGVASGIFSTFRYFGSIISSALIGLISGYHTLFMILFAV
HHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIIGVFVSLGIKSDETARIEKNSA
HHHHHHHHHCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377