Click here to switch to the map view.

The map label for this gene is lgt

Identifier: 16080552

GI number: 16080552

Start: 3593419

End: 3594228

Strand: Reverse

Name: lgt

Synonym: BSU34990

Alternate gene names: 16080552

Gene position: 3594228-3593419 (Counterclockwise)

Preceding gene: 16080553

Following gene: 16080551

Centisome position: 85.26

GC content: 46.67

Gene sequence:

>810_bases
ATGAATGAAGCGATAGAACCACTCAATCCGATAGCATTTCAGCTGGGGCCGTTAGCCGTCCACTGGTACGGAATCATTAT
CGGTCTCGGCGCTTTGCTCGGGCTGTGGATAGCGATGAGGGAAAGTGAAAAACGGGGGCTGCAGAAAGATACGTTTATTG
ATCTTGTCCTGTTTGCCATTCCAATTGCGATTATTTGCGCACGAATTTATTATGTTGCCTTTGAATGGGACTATTATGCG
GCACATCCGGGAGAAATCATTAAGATTTGGAAGGGCGGCATTGCCATTCATGGCGGATTAATCGGCGCCATTTTAACGGG
TTATGTGTTTTCGAGAGTGAAAAACCTTTCGTTCTGGAAGCTTGCGGATATTGCCGCGCCCAGCATTTTGCTCGGCCAGG
CGATCGGCCGCTGGGGGAACTTCATGAATCAGGAAGCGCACGGCGAGGCTGTCAGCAGAGCCTTTTTAGAAAACCTGCAC
CTGCCTGAATTTATCATCAATCAAATGTATATCAATGGGCAATATTATCATCCTACTTTTTTATATGAATCACTATGGAG
CTTTGTCGGCGTCATTGTTTTACTGCTGCTGCGCAGAGCGAATTTGCGTAGAGGCGAGATGTTCTTGATCTATATCATTT
GGTATTCAATCGGAAGATACTTTATTGAAGGAATGCGGACTGACAGCTTAATGCTGACTGATTCACTTCGCATCGCTCAG
GTGATTTCAATCGTATTGATTGTTTTGGCTGTGGCAGCCATCATCTTCAGACGCGTCAAGGGATACTCGAAGGAGCGGTA
CGCGGAGTAG

Upstream 100 bases:

>100_bases
AGATTGAAGCGGATGGGGCTGAATGCCGCTGAACAATTTACCAACAAGCTGGCGGACGTCATTGAAGACGGTGAACAAGA
AGAATAGGAGTTGGCGTTAA

Downstream 100 bases:

>100_bases
ACGTCAACAAAGGGGGATGAGGGATGAAGAAGATCTTTCTGGCCGGTCTTGCAGCAGGTTTTCAAACAACATGGACGCTG
GGTAAAGTGATTTTCCCGGT

Product: prolipoprotein diacylglyceryl transferase

Products: NA

Alternate protein names: Spore germination protein gerF

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNEAIEPLNPIAFQLGPLAVHWYGIIIGLGALLGLWIAMRESEKRGLQKDTFIDLVLFAIPIAIICARIYYVAFEWDYYA
AHPGEIIKIWKGGIAIHGGLIGAILTGYVFSRVKNLSFWKLADIAAPSILLGQAIGRWGNFMNQEAHGEAVSRAFLENLH
LPEFIINQMYINGQYYHPTFLYESLWSFVGVIVLLLLRRANLRRGEMFLIYIIWYSIGRYFIEGMRTDSLMLTDSLRIAQ
VISIVLIVLAVAAIIFRRVKGYSKERYAE

Sequences:

>Translated_269_residues
MNEAIEPLNPIAFQLGPLAVHWYGIIIGLGALLGLWIAMRESEKRGLQKDTFIDLVLFAIPIAIICARIYYVAFEWDYYA
AHPGEIIKIWKGGIAIHGGLIGAILTGYVFSRVKNLSFWKLADIAAPSILLGQAIGRWGNFMNQEAHGEAVSRAFLENLH
LPEFIINQMYINGQYYHPTFLYESLWSFVGVIVLLLLRRANLRRGEMFLIYIIWYSIGRYFIEGMRTDSLMLTDSLRIAQ
VISIVLIVLAVAAIIFRRVKGYSKERYAE
>Mature_269_residues
MNEAIEPLNPIAFQLGPLAVHWYGIIIGLGALLGLWIAMRESEKRGLQKDTFIDLVLFAIPIAIICARIYYVAFEWDYYA
AHPGEIIKIWKGGIAIHGGLIGAILTGYVFSRVKNLSFWKLADIAAPSILLGQAIGRWGNFMNQEAHGEAVSRAFLENLH
LPEFIINQMYINGQYYHPTFLYESLWSFVGVIVLLLLRRANLRRGEMFLIYIIWYSIGRYFIEGMRTDSLMLTDSLRIAQ
VISIVLIVLAVAAIIFRRVKGYSKERYAE

Specific function: Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins. Required for spore germination

COG id: COG0682

COG function: function code M; Prolipoprotein diacylglyceryltransferase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lgt family

Homologues:

Organism=Escherichia coli, GI1789192, Length=277, Percent_Identity=28.8808664259928, Blast_Score=103, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LGT_BACSU (O34752)

Other databases:

- EMBL:   U63310
- EMBL:   AF017113
- EMBL:   AL009126
- PIR:   C69651
- RefSeq:   NP_391379.1
- EnsemblBacteria:   EBBACT00000001706
- GeneID:   936609
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34990
- NMPDR:   fig|224308.1.peg.3505
- GenoList:   BSU34990
- GeneTree:   EBGT00050000000942
- HOGENOM:   HBG450278
- OMA:   CARAYYV
- PhylomeDB:   O34752
- ProtClustDB:   PRK12437
- BioCyc:   BSUB:BSU34990-MONOMER
- HAMAP:   MF_01147
- InterPro:   IPR001640
- TIGRFAMs:   TIGR00544

Pfam domain/function: PF01790 LGT

EC number: 2.4.99.- [C]

Molecular weight: Translated: 30620; Mature: 30620

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS01311 LGT

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x126485a0)-; HASH(0x12376984)-; HASH(0x122ba2f4)-; HASH(0x1271e0d0)-; HASH(0x11e71c04)-; HASH(0x40bf5ef4)-; HASH(0x40bf5a98)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEAIEPLNPIAFQLGPLAVHWYGIIIGLGALLGLWIAMRESEKRGLQKDTFIDLVLFAI
CCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
PIAIICARIYYVAFEWDYYAAHPGEIIKIWKGGIAIHGGLIGAILTGYVFSRVKNLSFWK
HHHHHHHHHHHHHEECCEEECCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCHHH
LADIAAPSILLGQAIGRWGNFMNQEAHGEAVSRAFLENLHLPEFIINQMYINGQYYHPTF
HHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEECHHH
LYESLWSFVGVIVLLLLRRANLRRGEMFLIYIIWYSIGRYFIEGMRTDSLMLTDSLRIAQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHH
VISIVLIVLAVAAIIFRRVKGYSKERYAE
HHHHHHHHHHHHHHHHHHHHCCCHHHCCH
>Mature Secondary Structure
MNEAIEPLNPIAFQLGPLAVHWYGIIIGLGALLGLWIAMRESEKRGLQKDTFIDLVLFAI
CCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
PIAIICARIYYVAFEWDYYAAHPGEIIKIWKGGIAIHGGLIGAILTGYVFSRVKNLSFWK
HHHHHHHHHHHHHEECCEEECCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCHHH
LADIAAPSILLGQAIGRWGNFMNQEAHGEAVSRAFLENLHLPEFIINQMYINGQYYHPTF
HHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEECHHH
LYESLWSFVGVIVLLLLRRANLRRGEMFLIYIIWYSIGRYFIEGMRTDSLMLTDSLRIAQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHH
VISIVLIVLAVAAIIFRRVKGYSKERYAE
HHHHHHHHHHHHHHHHHHHHCCCHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 10096076