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The map label for this gene is hprP

Identifier: 16080550

GI number: 16080550

Start: 3591810

End: 3592460

Strand: Reverse

Name: hprP

Synonym: BSU34970

Alternate gene names: 16080550

Gene position: 3592460-3591810 (Counterclockwise)

Preceding gene: 16080551

Following gene: 16080549

Centisome position: 85.22

GC content: 43.63

Gene sequence:

>651_bases
ATGAGTGACAAACAAGTAACGACGATTCTGTTTGATTTAGACGGAACGCTTATTAATACGAATGAATTAATTATCGCGTC
CTTTTTGCATACACTGGAGCATTATTATCCGAGCAAGTATAAACGAGAAGATGTCCTTGCATTTATCGGGCCGTCTCTTT
TTGATACGTTCTCTTCGATGGACCCTGATAAATGCGAGGATATGATCGCTATGTACAGAGCGTACAATCATGACATGCAT
GATTCACTCGTCACTGAATATGAAACGGTATATGAGACCCTGGATGCATTAAAAAAAGCCGGCTTTACATTAGGAATTGT
GACAACAAAATTAAGAGATACTGTCAATATGGGCCTCAAGCTCACGGGAATCGGCGAATTCTTTGAAACAGTTGTCACCC
TTGATGATGTGACGAATGCAAAGCCTGATCCCGAGCCTGTGCTGTTAGCGCTGAAACAGCTAGGAAGCGAGCCGGCGGAA
GCCATTATGGTCGGTGATAATTACCATGATGTGCTGGCAGGGAAAAACGCCGGTACAAAAACGGCAGGAGTCGCATGGAC
GATTAAAGGGCCGGAAATGCTGGCGAAGCATGAGCCGGATTTTATGCTTGAGAAAATGAGTGATCTATTACAAATCGTTG
GAGTGAAGTAA

Upstream 100 bases:

>100_bases
CGCTTCTTTTGATTCGTGTCACCACAGCAGTTCTGCTGACAATGGCTATTGCGCACACGTGGAAAAAATGGAAACCCTCA
GCGGTTGGAAAGGAAGCAAT

Downstream 100 bases:

>100_bases
TCTGTGAGAAAAACAGATCGTCATCCGGTCTCGGGGGCCAACTCACTGTGGCATGTCTATCAAACGGTACCTTTTTTAAA
GGTCGTCAAAAATTTCATTG

Product: pyrophosphatase PpaX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSDKQVTTILFDLDGTLINTNELIIASFLHTLEHYYPSKYKREDVLAFIGPSLFDTFSSMDPDKCEDMIAMYRAYNHDMH
DSLVTEYETVYETLDALKKAGFTLGIVTTKLRDTVNMGLKLTGIGEFFETVVTLDDVTNAKPDPEPVLLALKQLGSEPAE
AIMVGDNYHDVLAGKNAGTKTAGVAWTIKGPEMLAKHEPDFMLEKMSDLLQIVGVK

Sequences:

>Translated_216_residues
MSDKQVTTILFDLDGTLINTNELIIASFLHTLEHYYPSKYKREDVLAFIGPSLFDTFSSMDPDKCEDMIAMYRAYNHDMH
DSLVTEYETVYETLDALKKAGFTLGIVTTKLRDTVNMGLKLTGIGEFFETVVTLDDVTNAKPDPEPVLLALKQLGSEPAE
AIMVGDNYHDVLAGKNAGTKTAGVAWTIKGPEMLAKHEPDFMLEKMSDLLQIVGVK
>Mature_215_residues
SDKQVTTILFDLDGTLINTNELIIASFLHTLEHYYPSKYKREDVLAFIGPSLFDTFSSMDPDKCEDMIAMYRAYNHDMHD
SLVTEYETVYETLDALKKAGFTLGIVTTKLRDTVNMGLKLTGIGEFFETVVTLDDVTNAKPDPEPVLLALKQLGSEPAEA
IMVGDNYHDVLAGKNAGTKTAGVAWTIKGPEMLAKHEPDFMLEKMSDLLQIVGVK

Specific function: Hydrolyzes pyrophosphate formed during P-Ser-HPr dephosphorylation by HPrK/P. Might play a role in controlling the intracellular pyrophosphate pool

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. PpaX family

Homologues:

Organism=Escherichia coli, GI1789787, Length=237, Percent_Identity=25.3164556962025, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPAX_BACSU (Q9JMQ2)

Other databases:

- EMBL:   AF017113
- EMBL:   AL009126
- PIR:   G70044
- RefSeq:   NP_391377.1
- ProteinModelPortal:   Q9JMQ2
- SMR:   Q9JMQ2
- PptaseDB:   P3D0409133
- EnsemblBacteria:   EBBACT00000003231
- GeneID:   936613
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34970
- NMPDR:   fig|224308.1.peg.3503
- GenoList:   BSU34970
- GeneTree:   EBGT00050000000474
- HOGENOM:   HBG742904
- OMA:   QYEPDFM
- PhylomeDB:   Q9JMQ2
- ProtClustDB:   PRK13288
- BioCyc:   BSUB:BSU34970-MONOMER
- BRENDA:   3.6.1.1
- HAMAP:   MF_01250
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- Gene3D:   G3DSA:3.40.50.1000
- PRINTS:   PR00413
- TIGRFAMs:   TIGR01549
- TIGRFAMs:   TIGR01509

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.6.1.1

Molecular weight: Translated: 24004; Mature: 23873

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: ACT_SITE 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKQVTTILFDLDGTLINTNELIIASFLHTLEHYYPSKYKREDVLAFIGPSLFDTFSSM
CCCCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHCC
DPDKCEDMIAMYRAYNHDMHDSLVTEYETVYETLDALKKAGFTLGIVTTKLRDTVNMGLK
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCEE
LTGIGEFFETVVTLDDVTNAKPDPEPVLLALKQLGSEPAEAIMVGDNYHDVLAGKNAGTK
EEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHCCCCCCCC
TAGVAWTIKGPEMLAKHEPDFMLEKMSDLLQIVGVK
CCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDKQVTTILFDLDGTLINTNELIIASFLHTLEHYYPSKYKREDVLAFIGPSLFDTFSSM
CCCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHCC
DPDKCEDMIAMYRAYNHDMHDSLVTEYETVYETLDALKKAGFTLGIVTTKLRDTVNMGLK
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCEE
LTGIGEFFETVVTLDDVTNAKPDPEPVLLALKQLGSEPAEAIMVGDNYHDVLAGKNAGTK
EEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHCCCCCCCC
TAGVAWTIKGPEMLAKHEPDFMLEKMSDLLQIVGVK
CCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 9465101; 12359880