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The map label for this gene is yvaB

Identifier: 16080407

GI number: 16080407

Start: 3445442

End: 3446077

Strand: Reverse

Name: yvaB

Synonym: BSU33540

Alternate gene names: 16080407

Gene position: 3446077-3445442 (Counterclockwise)

Preceding gene: 16080408

Following gene: 16080405

Centisome position: 81.75

GC content: 43.55

Gene sequence:

>636_bases
ATGGCAAAAGTATTATATATCACTGCTCATCCACATGACGAAGCAACTTCGTACAGTATGGCAACAGGTAAAGCATTTAT
CGAATCTTACAAAGAAGCGAATCCAAATGATGAAGTTGTACATATCGATTTGTACAAAGAAAACATTCCGCACATTGACG
CAGATGTATTTAGCGGATGGGGAAAGCTTCAGTCAGGTACAGGCTTTGAAGAGCTTTCTGAAAGCGAAAAAGCGAAAGTA
GGCCGTTTGGGAGAGCTTAGCGATCAATTTGCATCAGCTGATAAGTACGTATTTGTTACTCCTTTATGGAACTTCTCATT
CCCGCCAGTGATGAAAGCGTATCTCGATTCTGTTGCTGTTGCAGGAAAATCATTCAAATACACAGAACAAGGTCCTGTCG
GCTTGTTAACTGATAAAAAAGCAATTCATATCCAAGCGCGCGGCGGCTACTATTCAGAAGGCCCTGCGGCTGAGATGGAA
ATGGGGCACCGCTACATCGGCATCATGATGAACTTCTTCGGCGTACCATCATTCGATGGCATTTTCGTAGAGGGACATAA
TGCGGAGCCTGATAAAGCACAGCAAATCAAAGAAGACGCGATTGCGCGTGCGAAAGAAGCAGGAAAAACATTCTAA

Upstream 100 bases:

>100_bases
TTTGTCTCTATAATAAAGTCTAAGAGAGACAGAATAATCATTATGCATCTGTATGATAATAATTGATGTGTGATTTTTAA
AAACGAAAGGGCTGGTAAAA

Downstream 100 bases:

>100_bases
TGAAAAAGCCTCCCCTTATTTGGGGAGGCTTTTGTTTTAGTGCTCCAGCATAATGGTTCGTTTCTCTTTACTGCTTTCCA
TCGCGGCTTCAATGATGCGG

Product: azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 2; FMN-dependent NADH-azo compound oxidoreductase 2

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MAKVLYITAHPHDEATSYSMATGKAFIESYKEANPNDEVVHIDLYKENIPHIDADVFSGWGKLQSGTGFEELSESEKAKV
GRLGELSDQFASADKYVFVTPLWNFSFPPVMKAYLDSVAVAGKSFKYTEQGPVGLLTDKKAIHIQARGGYYSEGPAAEME
MGHRYIGIMMNFFGVPSFDGIFVEGHNAEPDKAQQIKEDAIARAKEAGKTF

Sequences:

>Translated_211_residues
MAKVLYITAHPHDEATSYSMATGKAFIESYKEANPNDEVVHIDLYKENIPHIDADVFSGWGKLQSGTGFEELSESEKAKV
GRLGELSDQFASADKYVFVTPLWNFSFPPVMKAYLDSVAVAGKSFKYTEQGPVGLLTDKKAIHIQARGGYYSEGPAAEME
MGHRYIGIMMNFFGVPSFDGIFVEGHNAEPDKAQQIKEDAIARAKEAGKTF
>Mature_210_residues
AKVLYITAHPHDEATSYSMATGKAFIESYKEANPNDEVVHIDLYKENIPHIDADVFSGWGKLQSGTGFEELSESEKAKVG
RLGELSDQFASADKYVFVTPLWNFSFPPVMKAYLDSVAVAGKSFKYTEQGPVGLLTDKKAIHIQARGGYYSEGPAAEMEM
GHRYIGIMMNFFGVPSFDGIFVEGHNAEPDKAQQIKEDAIARAKEAGKTF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity. Confers resistance to catechol, 2-methylhydroquinone (2-MHQ), and diamide. Probably co

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=177, Percent_Identity=32.2033898305085, Blast_Score=95, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR2_BACSU (O32224)

Other databases:

- EMBL:   AL009126
- PIR:   H70026
- RefSeq:   NP_391234.1
- ProteinModelPortal:   O32224
- SMR:   O32224
- EnsemblBacteria:   EBBACT00000003610
- GeneID:   936142
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU33540
- NMPDR:   fig|224308.1.peg.3360
- GenoList:   BSU33540
- GeneTree:   EBGT00050000000772
- HOGENOM:   HBG565834
- OMA:   FSGWGKL
- PhylomeDB:   O32224
- ProtClustDB:   PRK00170
- BioCyc:   BSUB:BSU33540-MONOMER
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14

Molecular weight: Translated: 23272; Mature: 23141

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVLYITAHPHDEATSYSMATGKAFIESYKEANPNDEVVHIDLYKENIPHIDADVFSGW
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHCCC
GKLQSGTGFEELSESEKAKVGRLGELSDQFASADKYVFVTPLWNFSFPPVMKAYLDSVAV
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHH
AGKSFKYTEQGPVGLLTDKKAIHIQARGGYYSEGPAAEMEMGHRYIGIMMNFFGVPSFDG
CCCCCEECCCCCEEEEECCEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCCCE
IFVEGHNAEPDKAQQIKEDAIARAKEAGKTF
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKVLYITAHPHDEATSYSMATGKAFIESYKEANPNDEVVHIDLYKENIPHIDADVFSGW
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHCCC
GKLQSGTGFEELSESEKAKVGRLGELSDQFASADKYVFVTPLWNFSFPPVMKAYLDSVAV
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHH
AGKSFKYTEQGPVGLLTDKKAIHIQARGGYYSEGPAAEMEMGHRYIGIMMNFFGVPSFDG
CCCCCEECCCCCEEEEECCEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCCCE
IFVEGHNAEPDKAQQIKEDAIARAKEAGKTF
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377