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The map label for this gene is cysJ

Identifier: 16080397

GI number: 16080397

Start: 3432339

End: 3434156

Strand: Reverse

Name: cysJ

Synonym: BSU33440

Alternate gene names: 16080397

Gene position: 3434156-3432339 (Counterclockwise)

Preceding gene: 16080398

Following gene: 16080396

Centisome position: 81.46

GC content: 50.28

Gene sequence:

>1818_bases
TTGCAACTTCAGGTAATGAACAGTCCGTTTAATCAGGAGCAGGCAGAGCTCCTTAACCGCCTTCTGCCGACCTTGACCGA
ATCCCAGAAAATCTGGCTGAGCGGATATTTATCCGCTCAATCTGTTTCCGCCCAAGAAGCTGCGGGTACGCCGGCGGCAG
CTGTGTCAGCGGAAGCACCGGCACCGGCCGTTTCAAAAGAAGTGACCGTTCTTTATGGTTCACAGACGGGAAACGCACAG
GGGCTTGCAGAAAATGCCGGCAAGCAGCTTGAACAGAGCGGTTTTCAAGTGACCGTCTCATCCATGAGCGATTTTAAGCC
GAATCAGCTGAAGAAGGTAACCAATCTTTTAATCGTCGTCAGTACGCATGGAGAAGGCGAGCCGCCTGATAACGCGCTGT
CGTTTCATGAATTTCTGCACGGAAGACGGGCGCCTAAGCTTGAAGACCTGCGCTTTTCCGTTTTGGCGCTTGGTGACAGT
TCGTACGAATTTTTCTGCCAGACAGGGAAAGAATTTGATCAACGCTTAGAAGAACTCGGCGGAAAACGGATTTCTCCGCG
CGTTGATTGCGATCTTGACTATGATGAACCGGCAGCCGAATGGCTTGAGGGTGTATTGAAAGGATTAAATGAAGCTGGGG
GCGGAAGCGCCGCGCCTGCGCCGGCGGCTGCGTCACAAACCGGTGAGTCTTCGTATTCCAGAACAAACCCGTTTCGGGCT
GAAGTGTTAGAAAATTTAAATTTAAACGGCAGAGGATCAAATAAAGAAACACGCCATGTGGAGCTGTCTCTTGAAGGATC
TGGCCTGACTTACGAGCCTGGTGACAGCCTCGGCGTCTATCCGGAAAACGATCCGGAACTTGTCGAGCTGCTCCTTAAGG
AAATGAATTGGGACCCGGAAGAAATTGTGACGCTTAATAAGCAAGGTGATGTCCGACCGCTGAAAGAAGCGCTGATTTCT
CACTATGAGATTACCGTTCTCACAAAACCGCTTCTTGAACAGGCAGCCCAGCTGACAGGGAACGATGAGCTTAGAGAGCT
TTTAGCCCCGGGAAATGAGGAGAATGTAAAAGCCTATATTGAAGGGCGCGATTTGCTTGATCTCGTCCGTGACTATGGCC
CATTTAGTGTATCTGCGCAGGAGTTTGTCTCGATTTTGCGAAAAATGCCTGCCCGCCTTTATTCGATTGCAAGCAGTTTA
TCCGCAAATCCGGATGAAGTGCACTTAACAATCGGCGCAGTCCGCTACGATGCGCACGGCCGTGAACGAAAAGGCGTCTG
CTCGATTTTGTGCGCGGAACGACTGCAGCCGGGAGACACACTGCCGGTTTATGTACAGCACAATCAAAACTTTAAGCTTC
CGAAAGACCCTGAAACACCGATTATCATGGTGGGCCCTGGGACAGGCGTCGCACCTTTCCGTTCGTTTATGCAGGAGCGT
GAGGAGACGGGAGCGGAAGGAAAGGCATGGATGTTCTTTGGCGATCAGCACTTTGTCACGGATTTCCTTTACCAGACAGA
GTGGCAGAATTGGCTCAAAGACGGTGTGCTGACAAAAATGGATGTGGCGTTCTCACGTGATACGGAAGAAAAAGTATATG
TGCAGCACCGCATGCTTGAACATAGCGCAGAATTGTTTGAATGGCTTCAAGAAGGAGCGGCTGTTTACATTTGCGGAGAT
GAAAAGCACATGGCGCATGACGTCCATAACACATTGCTTGAAATTATCGAAAAAGAAGGCAATATGAGCCGCGAAGAGGC
GGAGGCTTATCTTGCCGATATGCAGCAGCAAAAACGCTATCAGCGTGATGTATATTGA

Upstream 100 bases:

>100_bases
GTTATCATCATGTAAATATATTTTACTTAAGCCTAGTATTCCGGTAAAGTTCAATTAGATGCAATTTTCGATCAGTTTAT
AACTTTGTGAGGTGGATACG

Downstream 100 bases:

>100_bases
TTTGACTTGAAAGGAGTTTTTCAACATGGTGACCAAAATTCTAAAAGCACCGGACGGCTCTCCAAGTGATGTTGAACGCA
TTAAAAAAGAAAGTGATTAT

Product: sulfite reductase flavoprotein alpha-subunit

Products: NA

Alternate protein names: SiR-FP

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MQLQVMNSPFNQEQAELLNRLLPTLTESQKIWLSGYLSAQSVSAQEAAGTPAAAVSAEAPAPAVSKEVTVLYGSQTGNAQ
GLAENAGKQLEQSGFQVTVSSMSDFKPNQLKKVTNLLIVVSTHGEGEPPDNALSFHEFLHGRRAPKLEDLRFSVLALGDS
SYEFFCQTGKEFDQRLEELGGKRISPRVDCDLDYDEPAAEWLEGVLKGLNEAGGGSAAPAPAAASQTGESSYSRTNPFRA
EVLENLNLNGRGSNKETRHVELSLEGSGLTYEPGDSLGVYPENDPELVELLLKEMNWDPEEIVTLNKQGDVRPLKEALIS
HYEITVLTKPLLEQAAQLTGNDELRELLAPGNEENVKAYIEGRDLLDLVRDYGPFSVSAQEFVSILRKMPARLYSIASSL
SANPDEVHLTIGAVRYDAHGRERKGVCSILCAERLQPGDTLPVYVQHNQNFKLPKDPETPIIMVGPGTGVAPFRSFMQER
EETGAEGKAWMFFGDQHFVTDFLYQTEWQNWLKDGVLTKMDVAFSRDTEEKVYVQHRMLEHSAELFEWLQEGAAVYICGD
EKHMAHDVHNTLLEIIEKEGNMSREEAEAYLADMQQQKRYQRDVY

Sequences:

>Translated_605_residues
MQLQVMNSPFNQEQAELLNRLLPTLTESQKIWLSGYLSAQSVSAQEAAGTPAAAVSAEAPAPAVSKEVTVLYGSQTGNAQ
GLAENAGKQLEQSGFQVTVSSMSDFKPNQLKKVTNLLIVVSTHGEGEPPDNALSFHEFLHGRRAPKLEDLRFSVLALGDS
SYEFFCQTGKEFDQRLEELGGKRISPRVDCDLDYDEPAAEWLEGVLKGLNEAGGGSAAPAPAAASQTGESSYSRTNPFRA
EVLENLNLNGRGSNKETRHVELSLEGSGLTYEPGDSLGVYPENDPELVELLLKEMNWDPEEIVTLNKQGDVRPLKEALIS
HYEITVLTKPLLEQAAQLTGNDELRELLAPGNEENVKAYIEGRDLLDLVRDYGPFSVSAQEFVSILRKMPARLYSIASSL
SANPDEVHLTIGAVRYDAHGRERKGVCSILCAERLQPGDTLPVYVQHNQNFKLPKDPETPIIMVGPGTGVAPFRSFMQER
EETGAEGKAWMFFGDQHFVTDFLYQTEWQNWLKDGVLTKMDVAFSRDTEEKVYVQHRMLEHSAELFEWLQEGAAVYICGD
EKHMAHDVHNTLLEIIEKEGNMSREEAEAYLADMQQQKRYQRDVY
>Mature_605_residues
MQLQVMNSPFNQEQAELLNRLLPTLTESQKIWLSGYLSAQSVSAQEAAGTPAAAVSAEAPAPAVSKEVTVLYGSQTGNAQ
GLAENAGKQLEQSGFQVTVSSMSDFKPNQLKKVTNLLIVVSTHGEGEPPDNALSFHEFLHGRRAPKLEDLRFSVLALGDS
SYEFFCQTGKEFDQRLEELGGKRISPRVDCDLDYDEPAAEWLEGVLKGLNEAGGGSAAPAPAAASQTGESSYSRTNPFRA
EVLENLNLNGRGSNKETRHVELSLEGSGLTYEPGDSLGVYPENDPELVELLLKEMNWDPEEIVTLNKQGDVRPLKEALIS
HYEITVLTKPLLEQAAQLTGNDELRELLAPGNEENVKAYIEGRDLLDLVRDYGPFSVSAQEFVSILRKMPARLYSIASSL
SANPDEVHLTIGAVRYDAHGRERKGVCSILCAERLQPGDTLPVYVQHNQNFKLPKDPETPIIMVGPGTGVAPFRSFMQER
EETGAEGKAWMFFGDQHFVTDFLYQTEWQNWLKDGVLTKMDVAFSRDTEEKVYVQHRMLEHSAELFEWLQEGAAVYICGD
EKHMAHDVHNTLLEIIEKEGNMSREEAEAYLADMQQQKRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain

Homologues:

Organism=Homo sapiens, GI127139033, Length=604, Percent_Identity=31.6225165562914, Blast_Score=262, Evalue=7e-70,
Organism=Homo sapiens, GI7657393, Length=599, Percent_Identity=31.5525876460768, Blast_Score=249, Evalue=7e-66,
Organism=Homo sapiens, GI221316705, Length=608, Percent_Identity=31.0855263157895, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI221316709, Length=599, Percent_Identity=31.3856427378965, Blast_Score=238, Evalue=9e-63,
Organism=Homo sapiens, GI24041029, Length=594, Percent_Identity=30.6397306397306, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI10835173, Length=667, Percent_Identity=28.4857571214393, Blast_Score=209, Evalue=6e-54,
Organism=Homo sapiens, GI40254422, Length=663, Percent_Identity=29.7134238310709, Blast_Score=209, Evalue=9e-54,
Organism=Homo sapiens, GI221316707, Length=522, Percent_Identity=31.2260536398467, Blast_Score=206, Evalue=6e-53,
Organism=Homo sapiens, GI169790956, Length=434, Percent_Identity=30.4147465437788, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI169790958, Length=437, Percent_Identity=30.2059496567506, Blast_Score=172, Evalue=9e-43,
Organism=Escherichia coli, GI1789123, Length=600, Percent_Identity=43.5, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI1790181, Length=140, Percent_Identity=31.4285714285714, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789154, Length=144, Percent_Identity=34.7222222222222, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554134, Length=595, Percent_Identity=32.1008403361345, Blast_Score=287, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17566446, Length=583, Percent_Identity=27.6157804459691, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17531441, Length=410, Percent_Identity=23.4146341463415, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321832, Length=655, Percent_Identity=28.7022900763359, Blast_Score=210, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6325305, Length=629, Percent_Identity=25.5961844197138, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321143, Length=390, Percent_Identity=28.4615384615385, Blast_Score=153, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6322597, Length=195, Percent_Identity=29.7435897435897, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17137192, Length=632, Percent_Identity=32.2784810126582, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24582192, Length=522, Percent_Identity=32.5670498084291, Blast_Score=239, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24660907, Length=591, Percent_Identity=28.9340101522843, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24660903, Length=591, Percent_Identity=28.9340101522843, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI78706872, Length=530, Percent_Identity=30.5660377358491, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24583543, Length=530, Percent_Identity=30.5660377358491, Blast_Score=177, Evalue=3e-44,
Organism=Drosophila melanogaster, GI78706876, Length=530, Percent_Identity=30.5660377358491, Blast_Score=177, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24647438, Length=416, Percent_Identity=25.2403846153846, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSJ_BACSU (O32214)

Other databases:

- EMBL:   AL009126
- PIR:   G70040
- RefSeq:   NP_391224.1
- HSSP:   P38038
- ProteinModelPortal:   O32214
- SMR:   O32214
- EnsemblBacteria:   EBBACT00000001826
- GeneID:   936022
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU33440
- NMPDR:   fig|224308.1.peg.3350
- GenoList:   BSU33440
- GeneTree:   EBGT00050000001044
- HOGENOM:   HBG736048
- OMA:   FHKDGAL
- PhylomeDB:   O32214
- ProtClustDB:   CLSK2752511
- BioCyc:   BSUB:BSU33440-MONOMER
- GO:   GO:0006810
- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938
- Gene3D:   G3DSA:1.20.990.10
- PIRSF:   PIRSF000207
- PRINTS:   PR00369
- PRINTS:   PR00371
- TIGRFAMs:   TIGR01931

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =1.8.1.2

Molecular weight: Translated: 67260; Mature: 67260

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS51384 FAD_FR; PS50902 FLAVODOXIN_LIKE

Important sites: BINDING 495-495

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLQVMNSPFNQEQAELLNRLLPTLTESQKIWLSGYLSAQSVSAQEAAGTPAAAVSAEAP
CEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCHHHHCCCCCHHCCCCCC
APAVSKEVTVLYGSQTGNAQGLAENAGKQLEQSGFQVTVSSMSDFKPNQLKKVTNLLIVV
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCEEEEE
STHGEGEPPDNALSFHEFLHGRRAPKLEDLRFSVLALGDSSYEFFCQTGKEFDQRLEELG
EECCCCCCCCHHHHHHHHHCCCCCCCHHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHHC
GKRISPRVDCDLDYDEPAAEWLEGVLKGLNEAGGGSAAPAPAAASQTGESSYSRTNPFRA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
EVLENLNLNGRGSNKETRHVELSLEGSGLTYEPGDSLGVYPENDPELVELLLKEMNWDPE
HHHHCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCHH
EIVTLNKQGDVRPLKEALISHYEITVLTKPLLEQAAQLTGNDELRELLAPGNEENVKAYI
HEEEECCCCCCHHHHHHHHCCHHHEEHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEE
EGRDLLDLVRDYGPFSVSAQEFVSILRKMPARLYSIASSLSANPDEVHLTIGAVRYDAHG
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCC
RERKGVCSILCAERLQPGDTLPVYVQHNQNFKLPKDPETPIIMVGPGTGVAPFRSFMQER
CCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
EETGAEGKAWMFFGDQHFVTDFLYQTEWQNWLKDGVLTKMDVAFSRDTEEKVYVQHRMLE
HHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHHH
HSAELFEWLQEGAAVYICGDEKHMAHDVHNTLLEIIEKEGNMSREEAEAYLADMQQQKRY
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QRDVY
HHCCC
>Mature Secondary Structure
MQLQVMNSPFNQEQAELLNRLLPTLTESQKIWLSGYLSAQSVSAQEAAGTPAAAVSAEAP
CEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCHHHHCCCCCHHCCCCCC
APAVSKEVTVLYGSQTGNAQGLAENAGKQLEQSGFQVTVSSMSDFKPNQLKKVTNLLIVV
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCEEEEE
STHGEGEPPDNALSFHEFLHGRRAPKLEDLRFSVLALGDSSYEFFCQTGKEFDQRLEELG
EECCCCCCCCHHHHHHHHHCCCCCCCHHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHHC
GKRISPRVDCDLDYDEPAAEWLEGVLKGLNEAGGGSAAPAPAAASQTGESSYSRTNPFRA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
EVLENLNLNGRGSNKETRHVELSLEGSGLTYEPGDSLGVYPENDPELVELLLKEMNWDPE
HHHHCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCHH
EIVTLNKQGDVRPLKEALISHYEITVLTKPLLEQAAQLTGNDELRELLAPGNEENVKAYI
HEEEECCCCCCHHHHHHHHCCHHHEEHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEE
EGRDLLDLVRDYGPFSVSAQEFVSILRKMPARLYSIASSLSANPDEVHLTIGAVRYDAHG
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCC
RERKGVCSILCAERLQPGDTLPVYVQHNQNFKLPKDPETPIIMVGPGTGVAPFRSFMQER
CCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
EETGAEGKAWMFFGDQHFVTDFLYQTEWQNWLKDGVLTKMDVAFSRDTEEKVYVQHRMLE
HHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHHH
HSAELFEWLQEGAAVYICGDEKHMAHDVHNTLLEIIEKEGNMSREEAEAYLADMQQQKRY
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QRDVY
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377