Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is fumC

Identifier: 16080357

GI number: 16080357

Start: 3389024

End: 3390412

Strand: Reverse

Name: fumC

Synonym: BSU33040

Alternate gene names: 16080357

Gene position: 3390412-3389024 (Counterclockwise)

Preceding gene: 255767753

Following gene: 255767752

Centisome position: 80.43

GC content: 47.66

Gene sequence:

>1389_bases
ATGGAATACAGAATTGAACGAGACACCATGGGAGAAGTAAAAGTTCCTGCTGATAAATTTTGGGGCGCCCAAACACAGCG
AAGCAAGGAGAACTTTAAGATCGGTTCTGAAAAAATGCCGATGCGTGTTGTGAAGGCGTTTGCAATTTTAAAACGAAGCA
CTGCTCTAGCTAATAAACGCCTCGGCAATCTGGACGCTGAAAAAGCAGAAGCGATTGCAGCGGTTTGTGATGATGTGCTC
AAAGGCAAGTACGATGACAACTTCCCGCTTGTCGTATGGCAGACCGGAAGCGGCACACAAAGCAATATGAACATGAACGA
AGTGGTAGCCAACCGTGCGACTGCTTTATTAAAAGAGAAGAACTCTGATCAAACGATTCATCCAAATGATGACGTGAACC
GAAGCCAAAGCTCAAACGACACATTCCCGACTGCGATGCATGTTGCCGCTGTTTTGGCTGTTTACGAGCAGCTTGTGCCG
GCGCTTGATCAGCTTCGCAATACACTGGATGAAAAAGCAAAAGCATACAATGATATTGTCAAAATCGGACGCACGCATCT
TCAGGATGCTACGCCACTGACCCTCGGACAGGAAATCAGCGGATGGGTTCACATGCTGGACCGTTCGAAGGAAATGATTT
TGGAAGCGACAGATAAAATGCGTGCGCTTGCAATTGGTGGAACGGCTGTCGGAACGGGTATCAACGCCCACCCAGAGTTT
GGAGAGCTTGTCTCAGAAGAGATCACTAAGCTGACCGGCCAAACGTTCAGCAGCTCGCCGAATAAATTCCACGCGCTGAC
AAGCCATGACGAAATCACTTATGCGCATGGCGCATTAAAAGCGCTTGCGGCTGACTTAATGAAAATCGCGAACGATGTCA
GATGGCTCGCAAGCGGACCCCGCTGCGGCATCGGGGAGATCGTCATTCCTGAAAATGAGCCGGGAAGCTCTATTATGCCT
GGTAAAGTCAATCCGACACAAAGCGAAGCGTTAACAATGATCGCAGCGCAAATCATGGGGAACGATGCGACAATCGGCTT
TGCGGCGAGTCAGGGGAACTTTGAACTGAACGTGTTTAAGCCTGTTATTATTTACAACTTCCTGCAATCTGTGCAGCTGC
TCAGTGACGGCATGAATTCATTCCATGATAAATGTGCTGTCGGTATTGAGCCTAATAAAGAAACCATTCAGGAAAACCTG
TCAAATTCATTAATGCTTGTTACGGCGCTGAACCCGCATATCGGTTATGAAAACGCGGCGAAAATCGCCAAGCTTGCCCA
TAAAGAGGGATTGACGCTCAAAGAAGCAGCTTTGAAGCTTGAATTGCTGACAGAGGAGCAATTTAACGAAATGGTTAAGC
CGGAAGACATGGTAAAACCAAAGGCGTAA

Upstream 100 bases:

>100_bases
ATTAAAATTAAAATCTGCCAAGATCGAAAAATAAAAAGGATTTTTTGTGTCATTGGCGAATTATGATCTATTGAAGCAAC
CGTTATGGAGGGATACATAA

Downstream 100 bases:

>100_bases
TAGGAAGAACGGCTGCTTTTTAAGCAGCCGTTCTTCTAAATATGTTGCTGTTTTAATAAATATTTAAGCTGTTCCGCATC
TTTGGCGAGAGCTTCAGACT

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MEYRIERDTMGEVKVPADKFWGAQTQRSKENFKIGSEKMPMRVVKAFAILKRSTALANKRLGNLDAEKAEAIAAVCDDVL
KGKYDDNFPLVVWQTGSGTQSNMNMNEVVANRATALLKEKNSDQTIHPNDDVNRSQSSNDTFPTAMHVAAVLAVYEQLVP
ALDQLRNTLDEKAKAYNDIVKIGRTHLQDATPLTLGQEISGWVHMLDRSKEMILEATDKMRALAIGGTAVGTGINAHPEF
GELVSEEITKLTGQTFSSSPNKFHALTSHDEITYAHGALKALAADLMKIANDVRWLASGPRCGIGEIVIPENEPGSSIMP
GKVNPTQSEALTMIAAQIMGNDATIGFAASQGNFELNVFKPVIIYNFLQSVQLLSDGMNSFHDKCAVGIEPNKETIQENL
SNSLMLVTALNPHIGYENAAKIAKLAHKEGLTLKEAALKLELLTEEQFNEMVKPEDMVKPKA

Sequences:

>Translated_462_residues
MEYRIERDTMGEVKVPADKFWGAQTQRSKENFKIGSEKMPMRVVKAFAILKRSTALANKRLGNLDAEKAEAIAAVCDDVL
KGKYDDNFPLVVWQTGSGTQSNMNMNEVVANRATALLKEKNSDQTIHPNDDVNRSQSSNDTFPTAMHVAAVLAVYEQLVP
ALDQLRNTLDEKAKAYNDIVKIGRTHLQDATPLTLGQEISGWVHMLDRSKEMILEATDKMRALAIGGTAVGTGINAHPEF
GELVSEEITKLTGQTFSSSPNKFHALTSHDEITYAHGALKALAADLMKIANDVRWLASGPRCGIGEIVIPENEPGSSIMP
GKVNPTQSEALTMIAAQIMGNDATIGFAASQGNFELNVFKPVIIYNFLQSVQLLSDGMNSFHDKCAVGIEPNKETIQENL
SNSLMLVTALNPHIGYENAAKIAKLAHKEGLTLKEAALKLELLTEEQFNEMVKPEDMVKPKA
>Mature_462_residues
MEYRIERDTMGEVKVPADKFWGAQTQRSKENFKIGSEKMPMRVVKAFAILKRSTALANKRLGNLDAEKAEAIAAVCDDVL
KGKYDDNFPLVVWQTGSGTQSNMNMNEVVANRATALLKEKNSDQTIHPNDDVNRSQSSNDTFPTAMHVAAVLAVYEQLVP
ALDQLRNTLDEKAKAYNDIVKIGRTHLQDATPLTLGQEISGWVHMLDRSKEMILEATDKMRALAIGGTAVGTGINAHPEF
GELVSEEITKLTGQTFSSSPNKFHALTSHDEITYAHGALKALAADLMKIANDVRWLASGPRCGIGEIVIPENEPGSSIMP
GKVNPTQSEALTMIAAQIMGNDATIGFAASQGNFELNVFKPVIIYNFLQSVQLLSDGMNSFHDKCAVGIEPNKETIQENL
SNSLMLVTALNPHIGYENAAKIAKLAHKEGLTLKEAALKLELLTEEQFNEMVKPEDMVKPKA

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=57.7922077922078, Blast_Score=534, Evalue=1e-152,
Organism=Escherichia coli, GI1787896, Length=456, Percent_Identity=64.0350877192982, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI87082375, Length=461, Percent_Identity=38.8286334056399, Blast_Score=327, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI17553882, Length=463, Percent_Identity=57.8833693304536, Blast_Score=550, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=58.4097859327217, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=57.0498915401301, Blast_Score=551, Evalue=1e-158,
Organism=Drosophila melanogaster, GI24640179, Length=464, Percent_Identity=56.4655172413793, Blast_Score=541, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24640177, Length=464, Percent_Identity=56.4655172413793, Blast_Score=540, Evalue=1e-154,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=54.7413793103448, Blast_Score=521, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=52.4731182795699, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=47.3002159827214, Blast_Score=435, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FUMC_BACSU (P07343)

Other databases:

- EMBL:   X01701
- EMBL:   AJ223978
- EMBL:   AL009126
- EMBL:   M11918
- EMBL:   Z93941
- PIR:   A23033
- RefSeq:   NP_391184.1
- ProteinModelPortal:   P07343
- SMR:   P07343
- EnsemblBacteria:   EBBACT00000000200
- GeneID:   938591
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU33040
- NMPDR:   fig|224308.1.peg.3310
- GenoList:   BSU33040
- GeneTree:   EBGT00070000031773
- HOGENOM:   HBG284369
- OMA:   RIEKDTM
- PhylomeDB:   P07343
- ProtClustDB:   PRK00485
- BioCyc:   BSUB:BSU33040-MONOMER
- BRENDA:   4.2.1.2
- HAMAP:   MF_00743
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00979

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.2.1.2

Molecular weight: Translated: 50532; Mature: 50532

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: BINDING 99-99

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MEYRIERDTMGEVKVPADKFWGAQTQRSKENFKIGSEKMPMRVVKAFAILKRSTALANKR
CCEEECCCCCCCEECCHHHHCCCCHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LGNLDAEKAEAIAAVCDDVLKGKYDDNFPLVVWQTGSGTQSNMNMNEVVANRATALLKEK
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHC
NSDQTIHPNDDVNRSQSSNDTFPTAMHVAAVLAVYEQLVPALDQLRNTLDEKAKAYNDIV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIGRTHLQDATPLTLGQEISGWVHMLDRSKEMILEATDKMRALAIGGTAVGTGINAHPEF
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHCCCCCCCCCHH
GELVSEEITKLTGQTFSSSPNKFHALTSHDEITYAHGALKALAADLMKIANDVRWLASGP
HHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RCGIGEIVIPENEPGSSIMPGKVNPTQSEALTMIAAQIMGNDATIGFAASQGNFELNVFK
CCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEHH
PVIIYNFLQSVQLLSDGMNSFHDKCAVGIEPNKETIQENLSNSLMLVTALNPHIGYENAA
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHH
KIAKLAHKEGLTLKEAALKLELLTEEQFNEMVKPEDMVKPKA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCC
>Mature Secondary Structure
MEYRIERDTMGEVKVPADKFWGAQTQRSKENFKIGSEKMPMRVVKAFAILKRSTALANKR
CCEEECCCCCCCEECCHHHHCCCCHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LGNLDAEKAEAIAAVCDDVLKGKYDDNFPLVVWQTGSGTQSNMNMNEVVANRATALLKEK
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHC
NSDQTIHPNDDVNRSQSSNDTFPTAMHVAAVLAVYEQLVPALDQLRNTLDEKAKAYNDIV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIGRTHLQDATPLTLGQEISGWVHMLDRSKEMILEATDKMRALAIGGTAVGTGINAHPEF
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHCCCCCCCCCHH
GELVSEEITKLTGQTFSSSPNKFHALTSHDEITYAHGALKALAADLMKIANDVRWLASGP
HHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RCGIGEIVIPENEPGSSIMPGKVNPTQSEALTMIAAQIMGNDATIGFAASQGNFELNVFK
CCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEHH
PVIIYNFLQSVQLLSDGMNSFHDKCAVGIEPNKETIQENLSNSLMLVTALNPHIGYENAA
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHH
KIAKLAHKEGLTLKEAALKLELLTEEQFNEMVKPEDMVKPKA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3923430; 9639930; 9384377; 2998952; 2509423; 9353931