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The map label for this gene is yueD

Identifier: 16080235

GI number: 16080235

Start: 3265406

End: 3266137

Strand: Reverse

Name: yueD

Synonym: BSU31840

Alternate gene names: 16080235

Gene position: 3266137-3265406 (Counterclockwise)

Preceding gene: 16080236

Following gene: 16080234

Centisome position: 77.48

GC content: 45.77

Gene sequence:

>732_bases
ATGGAACTTTATATCATCACCGGAGCGTCAAAAGGGCTGGGTCAAGCCATTGCATTACAGGCTTTAGAAAAGGGGCATGA
AGTCCATGCCTTATCCAGAACGAAAACAGATGTCTCTCACAAAAAACTAACGCAGCATCAAATAGACCTCATCAATCTCG
AAGAAGCTGAACAGCAATTTGAAACATTGCTCTCATCCATCGATTCAGATCGTTATTCTGGTATTACCCTTATTAATAAC
GCCGGAATGGTAACGCCGATCAAACGTGCCGGCGAAGCGTCTCTTGACGAGCTTCAGCGCCATTATCAGCTGAACCTGAC
TGCGCCCGTGCTTTTGAGTCAGCTGTTTACAAAACGGTTTGCTTCATACAGCGGCAAAAAGACGGTTGTCAACATTACTT
CAGGAGCCGCCAAAAATCCATATAAGGGATGGAGCGCGTATTGCAGTTCAAAAGCCGGGCTCGACATGTTTACGAGGACA
TTCGGATTTGAACAGGAGGATGAAGAGCTGCCGGTGAACATGATTTCGTTCTCACCTGGAGTGATGGACACTGAGATGCA
GGCCGTCATCCGTTCTTCATCGAAAAAGGATTTCCACCACATTGAACGATTCCGGAAATTAAATGAAACAGGAAGCCTTC
GCAGTCCGGACTTTATTGCCGGCACGCTGCTTTCTTTACTAGAAAAAGGGACGGAAAACGGCCGCATTTATGATATTAAA
GAGTTTTTGTAG

Upstream 100 bases:

>100_bases
TCCCAAAAGTCACAGCGCAGGAAAACAAGAAGGCGTAGGCCGCGTGCTTGTGTTGTGTAAAAGAGGTGCCGTACAATAAT
AACGGAAAAGGAGGTTTCCA

Downstream 100 bases:

>100_bases
AGCAGATATTTTCTGCTCTTTTTTTTATACAAAAGGCTGCAGCCAGCGCATTTTATTGAACAGCCGCATATACTGACTGA
AACGTTGCTTTTAGGGTATA

Product: short chain dehydrogenase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MELYIITGASKGLGQAIALQALEKGHEVHALSRTKTDVSHKKLTQHQIDLINLEEAEQQFETLLSSIDSDRYSGITLINN
AGMVTPIKRAGEASLDELQRHYQLNLTAPVLLSQLFTKRFASYSGKKTVVNITSGAAKNPYKGWSAYCSSKAGLDMFTRT
FGFEQEDEELPVNMISFSPGVMDTEMQAVIRSSSKKDFHHIERFRKLNETGSLRSPDFIAGTLLSLLEKGTENGRIYDIK
EFL

Sequences:

>Translated_243_residues
MELYIITGASKGLGQAIALQALEKGHEVHALSRTKTDVSHKKLTQHQIDLINLEEAEQQFETLLSSIDSDRYSGITLINN
AGMVTPIKRAGEASLDELQRHYQLNLTAPVLLSQLFTKRFASYSGKKTVVNITSGAAKNPYKGWSAYCSSKAGLDMFTRT
FGFEQEDEELPVNMISFSPGVMDTEMQAVIRSSSKKDFHHIERFRKLNETGSLRSPDFIAGTLLSLLEKGTENGRIYDIK
EFL
>Mature_243_residues
MELYIITGASKGLGQAIALQALEKGHEVHALSRTKTDVSHKKLTQHQIDLINLEEAEQQFETLLSSIDSDRYSGITLINN
AGMVTPIKRAGEASLDELQRHYQLNLTAPVLLSQLFTKRFASYSGKKTVVNITSGAAKNPYKGWSAYCSSKAGLDMFTRT
FGFEQEDEELPVNMISFSPGVMDTEMQAVIRSSSKKDFHHIERFRKLNETGSLRSPDFIAGTLLSLLEKGTENGRIYDIK
EFL

Specific function: Reduces benzil stereospecifically to (S)-benzoin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family

Homologues:

Organism=Homo sapiens, GI4507185, Length=158, Percent_Identity=34.8101265822785, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI7705925, Length=198, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1786812, Length=225, Percent_Identity=28.4444444444444, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115534694, Length=213, Percent_Identity=30.0469483568075, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17562910, Length=245, Percent_Identity=27.7551020408163, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17560332, Length=248, Percent_Identity=27.0161290322581, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322227, Length=248, Percent_Identity=27.8225806451613, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322226, Length=242, Percent_Identity=26.4462809917355, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323882, Length=203, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24640597, Length=240, Percent_Identity=28.75, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BZRD_BACSU (O32099)

Other databases:

- EMBL:   AL009126
- PIR:   E70007
- RefSeq:   NP_391062.1
- HSSP:   Q64105
- ProteinModelPortal:   O32099
- SMR:   O32099
- EnsemblBacteria:   EBBACT00000001778
- GeneID:   936558
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31840
- NMPDR:   fig|224308.1.peg.3187
- GenoList:   BSU31840
- GeneTree:   EBGT00050000000462
- HOGENOM:   HBG750976
- OMA:   GVIPRIA
- PhylomeDB:   O32099
- ProtClustDB:   PRK06924
- BioCyc:   BSUB:BSU31840-MONOMER
- GO:   GO:0005737
- GO:   GO:0005488
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR19410
- PRINTS:   PR00081

Pfam domain/function: PF00106 adh_short

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 27109; Mature: 27109

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00061 ADH_SHORT

Important sites: ACT_SITE 147-147 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELYIITGASKGLGQAIALQALEKGHEVHALSRTKTDVSHKKLTQHQIDLINLEEAEQQF
CEEEEEECCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHEECHHHHHHHH
ETLLSSIDSDRYSGITLINNAGMVTPIKRAGEASLDELQRHYQLNLTAPVLLSQLFTKRF
HHHHHHHCCCCCCCEEEECCCCCCCCHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
ASYSGKKTVVNITSGAAKNPYKGWSAYCSSKAGLDMFTRTFGFEQEDEELPVNMISFSPG
HHCCCCEEEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCC
VMDTEMQAVIRSSSKKDFHHIERFRKLNETGSLRSPDFIAGTLLSLLEKGTENGRIYDIK
CCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEHH
EFL
HCC
>Mature Secondary Structure
MELYIITGASKGLGQAIALQALEKGHEVHALSRTKTDVSHKKLTQHQIDLINLEEAEQQF
CEEEEEECCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHEECHHHHHHHH
ETLLSSIDSDRYSGITLINNAGMVTPIKRAGEASLDELQRHYQLNLTAPVLLSQLFTKRF
HHHHHHHCCCCCCCEEEECCCCCCCCHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
ASYSGKKTVVNITSGAAKNPYKGWSAYCSSKAGLDMFTRTFGFEQEDEELPVNMISFSPG
HHCCCCEEEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCC
VMDTEMQAVIRSSSKKDFHHIERFRKLNETGSLRSPDFIAGTLLSLLEKGTENGRIYDIK
CCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEHH
EFL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377