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The map label for this gene is kapD

Identifier: 16080199

GI number: 16080199

Start: 3231812

End: 3232429

Strand: Reverse

Name: kapD

Synonym: BSU31470

Alternate gene names: 16080199

Gene position: 3232429-3231812 (Counterclockwise)

Preceding gene: 255767717

Following gene: 255767714

Centisome position: 76.68

GC content: 41.26

Gene sequence:

>618_bases
GTGACGACAAACAGCTTACTCATTATTGATTTCGAATTTACAATGCCTGATGGAAAGTACAGTCCTCAAAACTTCTTCCC
GGAAATCATTGAAGCCGGAATTGTAAAGTCGATTGATGATGAAGTGGTTGAGACGTTTTCTAGCTATGTTAGACCGAAAA
AGTTCCCGAAGCTTACGAAACGCTGCAAATCATTTTTAAAAATAACCCAAAAACAGGTAGACGAAGGAATGAGATTCGAG
GATTTCATCCGTAAACTAAACGAGCTGGATCCTGAGAAAAACAGCACAATTATTACGTGGGGAAACATGGATATGAAAGT
GCTGAAGCAAAACTGTATGTTTAACCATATCCCTTTCCCGTTTAAGGGAGAAATGAGAGATTTATCGCTTGAATACAAAA
ACTTTTTTGGAGACCGGACATTGACGGGTTTATGGAAAGCTGCCGAAGAGTATGGAGATTCCGGAACCGGCACCCATCAT
AAAGCGCTTGATGATGCTCTCACAGCATATAAACTTTTTAAGCTTGTTGAGCAGGATAAGCAGTATCTCGAAAAGCCAAA
ACCGCCCACAATCGGCGAGAGAATCGATTTAACCGAACTGTTGAAGCGCGCGACGTAA

Upstream 100 bases:

>100_bases
GAAAATTCATTATTTTTAGAAAATGAAGAAATGTCGAGAAGGGGAATTGATATACTATAAAGATAGCATTTTCTGATCGG
AGGAAAGGCAGGTGTAAAAG

Downstream 100 bases:

>100_bases
AAAACCCAATCTTTTGTCAGACTGGGTTATGGCCGGTTAAAATATTCTTTTTCTAAAATGTCCAGATTATGAAGGGAGCG
CTTTGCCCACTCCGAACCAT

Product: sporulation inhibitor KapD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTTNSLLIIDFEFTMPDGKYSPQNFFPEIIEAGIVKSIDDEVVETFSSYVRPKKFPKLTKRCKSFLKITQKQVDEGMRFE
DFIRKLNELDPEKNSTIITWGNMDMKVLKQNCMFNHIPFPFKGEMRDLSLEYKNFFGDRTLTGLWKAAEEYGDSGTGTHH
KALDDALTAYKLFKLVEQDKQYLEKPKPPTIGERIDLTELLKRAT

Sequences:

>Translated_205_residues
MTTNSLLIIDFEFTMPDGKYSPQNFFPEIIEAGIVKSIDDEVVETFSSYVRPKKFPKLTKRCKSFLKITQKQVDEGMRFE
DFIRKLNELDPEKNSTIITWGNMDMKVLKQNCMFNHIPFPFKGEMRDLSLEYKNFFGDRTLTGLWKAAEEYGDSGTGTHH
KALDDALTAYKLFKLVEQDKQYLEKPKPPTIGERIDLTELLKRAT
>Mature_204_residues
TTNSLLIIDFEFTMPDGKYSPQNFFPEIIEAGIVKSIDDEVVETFSSYVRPKKFPKLTKRCKSFLKITQKQVDEGMRFED
FIRKLNELDPEKNSTIITWGNMDMKVLKQNCMFNHIPFPFKGEMRDLSLEYKNFFGDRTLTGLWKAAEEYGDSGTGTHHK
ALDDALTAYKLFKLVEQDKQYLEKPKPPTIGERIDLTELLKRAT

Specific function: Specifically inhibits the kinA pathway to sporulation

COG id: COG5018

COG function: function code R; Inhibitor of the KinA pathway to sporulation, predicted exonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain

Homologues:

Organism=Homo sapiens, GI31543184, Length=185, Percent_Identity=31.8918918918919, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17541516, Length=187, Percent_Identity=26.7379679144385, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KAPD_BACSU (O05231)

Other databases:

- EMBL:   U63302
- EMBL:   Z93933
- EMBL:   AL009126
- PIR:   B69647
- RefSeq:   NP_391025.1
- HSSP:   Q8IV48
- ProteinModelPortal:   O05231
- SMR:   O05231
- EnsemblBacteria:   EBBACT00000001441
- GeneID:   937171
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31470
- NMPDR:   fig|224308.1.peg.3150
- GenoList:   BSU31470
- GeneTree:   EBGT00050000001314
- HOGENOM:   HBG338263
- OMA:   NFFPEII
- ProtClustDB:   PRK07748
- BioCyc:   BSUB:BSU31470-MONOMER
- GO:   GO:0005622
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR012337
- SMART:   SM00479

Pfam domain/function: PF00929 Exonuc_X-T; SSF53098 RNaseH_fold

EC number: NA

Molecular weight: Translated: 23824; Mature: 23692

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 160-160 BINDING 12-12 BINDING 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNSLLIIDFEFTMPDGKYSPQNFFPEIIEAGIVKSIDDEVVETFSSYVRPKKFPKLTK
CCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RCKSFLKITQKQVDEGMRFEDFIRKLNELDPEKNSTIITWGNMDMKVLKQNCMFNHIPFP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCC
FKGEMRDLSLEYKNFFGDRTLTGLWKAAEEYGDSGTGTHHKALDDALTAYKLFKLVEQDK
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QYLEKPKPPTIGERIDLTELLKRAT
HHHHCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
TTNSLLIIDFEFTMPDGKYSPQNFFPEIIEAGIVKSIDDEVVETFSSYVRPKKFPKLTK
CCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RCKSFLKITQKQVDEGMRFEDFIRKLNELDPEKNSTIITWGNMDMKVLKQNCMFNHIPFP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCC
FKGEMRDLSLEYKNFFGDRTLTGLWKAAEEYGDSGTGTHHKALDDALTAYKLFKLVEQDK
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QYLEKPKPPTIGERIDLTELLKRAT
HHHHCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377