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The map label for this gene is menB

Identifier: 16080132

GI number: 16080132

Start: 3148901

End: 3149716

Strand: Reverse

Name: menB

Synonym: BSU30800

Alternate gene names: 16080132

Gene position: 3149716-3148901 (Counterclockwise)

Preceding gene: 16080133

Following gene: 16080131

Centisome position: 74.72

GC content: 47.43

Gene sequence:

>816_bases
ATGGCTGAATGGAAAACAAAACGGACATACGATGAGATATTGTATGAAACGTATAATGGCATTGCAAAAATAACAATCAA
CCGACCTGAGGTACATAATGCGTTTACCCCTAAAACGGTTGCTGAAATGATTGATGCGTTTGCTGACGCAAGAGACGACC
AAAACGTTGGGGTTATCGTGCTTGCCGGTGCAGGGGACAAAGCATTTTGTTCTGGCGGAGACCAAAAAGTGCGCGGCCAC
GGTGGATATGTAGGAGACGACCAGATCCCTCGTCTTAACGTATTGGATCTTCAGCGTTTAATCCGCGTCATCCCGAAACC
GGTTGTTGCGATGGTGTCCGGATATGCGATCGGCGGAGGCCATGTGCTTCACATCGTATGTGACTTGACAATTGCTGCGG
ACAACGCAATTTTTGGACAAACAGGCCCTAAAGTGGGAAGCTTCGATGCAGGTTACGGTTCTGGCTACCTGGCTCGAATT
GTAGGACATAAAAAAGCACGTGAAATCTGGTACCTATGCCGTCAGTACAACGCACAGGAAGCACTGGACATGGGTCTTGT
CAACACAGTCGTTCCTTTGGAACAGCTTGAAGAAGAAACGATTAAATGGTGTGAAGAAATGCTTGAAAAAAGCCCGACCG
CACTGCGCTTTCTTAAAGCTGCGTTTAACGCGGACACAGACGGACTTGCTGGAATTCAGCAGTTTGCAGGGGATGCTACC
CTTCTTTACTACACAACAGATGAAGCAAAAGAAGGCCGTGATTCCTTTAAGGAAAAACGCAAACCTGATTTCGGACAGTT
CCCTCGTTTTCCGTGA

Upstream 100 bases:

>100_bases
ACGGTCCATGTGGAGCAGCCGCGATTGTTTGGTAAAATAGTGAGTGAGTTTTTGACAAGCATCTGACTCATTCACATAGA
GAATAAAAGGAGGTCATCAT

Downstream 100 bases:

>100_bases
TCAGCAATATCTAGTAAACCAACAGCTTGAGACTTTGCGGTCCAAGCTGTTTTCTTTTCAATACAGACATTTTACCTCGG
AGATGATGACATGCTGACAG

Product: naphthoate synthase

Products: NA

Alternate protein names: DHNA-CoA synthase

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MAEWKTKRTYDEILYETYNGIAKITINRPEVHNAFTPKTVAEMIDAFADARDDQNVGVIVLAGAGDKAFCSGGDQKVRGH
GGYVGDDQIPRLNVLDLQRLIRVIPKPVVAMVSGYAIGGGHVLHIVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLARI
VGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEQLEEETIKWCEEMLEKSPTALRFLKAAFNADTDGLAGIQQFAGDAT
LLYYTTDEAKEGRDSFKEKRKPDFGQFPRFP

Sequences:

>Translated_271_residues
MAEWKTKRTYDEILYETYNGIAKITINRPEVHNAFTPKTVAEMIDAFADARDDQNVGVIVLAGAGDKAFCSGGDQKVRGH
GGYVGDDQIPRLNVLDLQRLIRVIPKPVVAMVSGYAIGGGHVLHIVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLARI
VGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEQLEEETIKWCEEMLEKSPTALRFLKAAFNADTDGLAGIQQFAGDAT
LLYYTTDEAKEGRDSFKEKRKPDFGQFPRFP
>Mature_270_residues
AEWKTKRTYDEILYETYNGIAKITINRPEVHNAFTPKTVAEMIDAFADARDDQNVGVIVLAGAGDKAFCSGGDQKVRGHG
GYVGDDQIPRLNVLDLQRLIRVIPKPVVAMVSGYAIGGGHVLHIVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLARIV
GHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEQLEEETIKWCEEMLEKSPTALRFLKAAFNADTDGLAGIQQFAGDATL
LYYTTDEAKEGRDSFKEKRKPDFGQFPRFP

Specific function: Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4- dihydroxy-2-naphthoyl-CoA (DHNA-CoA)

COG id: COG0447

COG function: function code H; Dihydroxynaphthoic acid synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=33.8709677419355, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI31542718, Length=262, Percent_Identity=29.0076335877863, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4502327, Length=279, Percent_Identity=30.1075268817204, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI45643119, Length=213, Percent_Identity=27.6995305164319, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI260275230, Length=213, Percent_Identity=27.6995305164319, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI260274832, Length=213, Percent_Identity=27.6995305164319, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI70995211, Length=217, Percent_Identity=29.4930875576037, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI37594469, Length=154, Percent_Identity=27.9220779220779, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI37594471, Length=154, Percent_Identity=27.9220779220779, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788597, Length=272, Percent_Identity=68.75, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI221142681, Length=268, Percent_Identity=31.3432835820896, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87082183, Length=266, Percent_Identity=28.9473684210526, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=29.3436293436293, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=28.6821705426357, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=30.8943089430894, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=31.3492063492063, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=207, Percent_Identity=29.4685990338164, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=216, Percent_Identity=28.2407407407407, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=219, Percent_Identity=29.6803652968037, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=181, Percent_Identity=30.939226519337, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=211, Percent_Identity=26.5402843601896, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=261, Percent_Identity=27.2030651340996, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=166, Percent_Identity=26.5060240963855, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=166, Percent_Identity=26.5060240963855, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=33.7448559670782, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=33.7448559670782, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=29.6992481203008, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21357171, Length=187, Percent_Identity=33.6898395721925, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922422, Length=262, Percent_Identity=26.7175572519084, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24650670, Length=192, Percent_Identity=30.2083333333333, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24654903, Length=200, Percent_Identity=27.5, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENB_BACSU (P23966)

Other databases:

- EMBL:   M74521
- EMBL:   M74538
- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   F69656
- RefSeq:   NP_390958.1
- ProteinModelPortal:   P23966
- SMR:   P23966
- EnsemblBacteria:   EBBACT00000001509
- GeneID:   937195
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30800
- NMPDR:   fig|224308.1.peg.3083
- GenoList:   BSU30800
- GeneTree:   EBGT00070000031824
- HOGENOM:   HBG748731
- OMA:   GEDDIPR
- PhylomeDB:   P23966
- ProtClustDB:   PRK07396
- BioCyc:   BSUB:BSU30800-MONOMER
- BioCyc:   MetaCyc:MONOMER-13812
- BRENDA:   4.1.3.36
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376
- InterPro:   IPR010198
- Gene3D:   G3DSA:1.10.12.10
- TIGRFAMs:   TIGR01929

Pfam domain/function: PF00378 ECH

EC number: =4.1.3.36

Molecular weight: Translated: 29899; Mature: 29768

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: BINDING 141-141 BINDING 147-147

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEWKTKRTYDEILYETYNGIAKITINRPEVHNAFTPKTVAEMIDAFADARDDQNVGVIV
CCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEE
LAGAGDKAFCSGGDQKVRGHGGYVGDDQIPRLNVLDLQRLIRVIPKPVVAMVSGYAIGGG
EECCCCCHHHCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCC
HVLHIVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLARIVGHKKAREIWYLCRQYNAQE
EEEEEEEEEEEECCCCEECCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCHHH
ALDMGLVNTVVPLEQLEEETIKWCEEMLEKSPTALRFLKAAFNADTDGLAGIQQFAGDAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCEE
LLYYTTDEAKEGRDSFKEKRKPDFGQFPRFP
EEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AEWKTKRTYDEILYETYNGIAKITINRPEVHNAFTPKTVAEMIDAFADARDDQNVGVIV
CCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEE
LAGAGDKAFCSGGDQKVRGHGGYVGDDQIPRLNVLDLQRLIRVIPKPVVAMVSGYAIGGG
EECCCCCHHHCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCC
HVLHIVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLARIVGHKKAREIWYLCRQYNAQE
EEEEEEEEEEEECCCCEECCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCHHH
ALDMGLVNTVVPLEQLEEETIKWCEEMLEKSPTALRFLKAAFNADTDGLAGIQQFAGDAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCEE
LLYYTTDEAKEGRDSFKEKRKPDFGQFPRFP
EEEEECCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377