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The map label for this gene is ytpR

Identifier: 16080034

GI number: 16080034

Start: 3052743

End: 3053348

Strand: Reverse

Name: ytpR

Synonym: BSU29820

Alternate gene names: 16080034

Gene position: 3053348-3052743 (Counterclockwise)

Preceding gene: 16080035

Following gene: 255767684

Centisome position: 72.43

GC content: 44.22

Gene sequence:

>606_bases
ATGAACGCTTTTTATAATAAAGAAGGTGTGGGAGACACGCTCCTGATCTCTCTTCAAGATGTGACACGCGAACAATTAGG
CTATGAAAAACACGGCGACGTCGTCAAAATTTTCAATAACGAAACAAAAGAAACAACGGGCTTCAACATTTTCAATGCGT
CTTCTTACTTGACCATTGATGAAAACGGCCCTGTTGCGCTCTCAGAAACATTCGTGCAAGATGTGAATGAGATTTTAAAC
AGAAACGGCGTTGAAGAAACATTAGTCGTTGATTTATCTCCGAAGTTTGTTGTGGGTTATGTAGAATCAAAAGAAAAACA
TCCGAATGCGGATAAATTAAGCGTATGTAAAGTCAACGTAGGAGAAGAAACGCTTCAGATCGTCTGCGGCGCGCCTAACG
TTGACCAAGGCCAAAAAGTTGTCGTTGCCAAAGTCGGTGCTGTGATGCCTAGCGGACTTGTCATTAAGGATGCAGAGCTT
CGTGGCGTTCCGTCAAGCGGGATGATCTGCTCAGCGAAAGAGCTTGATTTGCCGGATGCCCCTGCTGAAAAAGGAATCCT
TGTGTTAGAAGGAGACTATGAGGCGGGAGACGCATTTCAGTTTTAG

Upstream 100 bases:

>100_bases
GATTACCGCCCTATCATTTTTGTACAATGAAGGAAAGCTGGAACCGGTGTTTATCCTGGCGAAAAGCCGTCCGAAAAAGG
ATTAGAAAGGATTTTTACTT

Downstream 100 bases:

>100_bases
ACTCCCATAAATCCGCCAAAAAAAACGAATCATGTGAAAAGCCGCTAAGCCCTTGTTTAGCGGCTTTGTTTCTGCTGATA
GCTTAATCTTTTCTGGGCCG

Product: hypothetical protein

Products: AMP; diphosphate; L-phenylalanyl-tRNAPhe [C]

Alternate protein names: Partial phenylalanyl-tRNA synthetase; Partial PheRS; pFRS

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MNAFYNKEGVGDTLLISLQDVTREQLGYEKHGDVVKIFNNETKETTGFNIFNASSYLTIDENGPVALSETFVQDVNEILN
RNGVEETLVVDLSPKFVVGYVESKEKHPNADKLSVCKVNVGEETLQIVCGAPNVDQGQKVVVAKVGAVMPSGLVIKDAEL
RGVPSSGMICSAKELDLPDAPAEKGILVLEGDYEAGDAFQF

Sequences:

>Translated_201_residues
MNAFYNKEGVGDTLLISLQDVTREQLGYEKHGDVVKIFNNETKETTGFNIFNASSYLTIDENGPVALSETFVQDVNEILN
RNGVEETLVVDLSPKFVVGYVESKEKHPNADKLSVCKVNVGEETLQIVCGAPNVDQGQKVVVAKVGAVMPSGLVIKDAEL
RGVPSSGMICSAKELDLPDAPAEKGILVLEGDYEAGDAFQF
>Mature_201_residues
MNAFYNKEGVGDTLLISLQDVTREQLGYEKHGDVVKIFNNETKETTGFNIFNASSYLTIDENGPVALSETFVQDVNEILN
RNGVEETLVVDLSPKFVVGYVESKEKHPNADKLSVCKVNVGEETLQIVCGAPNVDQGQKVVVAKVGAVMPSGLVIKDAEL
RGVPSSGMICSAKELDLPDAPAEKGILVLEGDYEAGDAFQF

Specific function: Unknown

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788006, Length=92, Percent_Identity=54.3478260869565, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): YTPR_BACSU (O34943)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   A69999
- RefSeq:   NP_390860.1
- HSSP:   P42589
- ProteinModelPortal:   O34943
- SMR:   O34943
- EnsemblBacteria:   EBBACT00000000292
- GeneID:   937964
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29820
- NMPDR:   fig|224308.1.peg.2985
- GenoList:   BSU29820
- GeneTree:   EBGT00050000001777
- HOGENOM:   HBG571920
- OMA:   GAMMPNG
- ProtClustDB:   CLSK887731
- BioCyc:   BSUB:BSU29820-MONOMER
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR002547
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB

EC number: 6.1.1.20 [C]

Molecular weight: Translated: 21697; Mature: 21697

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFYNKEGVGDTLLISLQDVTREQLGYEKHGDVVKIFNNETKETTGFNIFNASSYLTID
CCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCEEEEEC
ENGPVALSETFVQDVNEILNRNGVEETLVVDLSPKFVVGYVESKEKHPNADKLSVCKVNV
CCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCEEEEEEEC
GEETLQIVCGAPNVDQGQKVVVAKVGAVMPSGLVIKDAELRGVPSSGMICSAKELDLPDA
CCCEEEEEECCCCCCCCCEEEEEEECCCCCCCEEEECCHHCCCCCCCCEEEHHCCCCCCC
PAEKGILVLEGDYEAGDAFQF
CCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNAFYNKEGVGDTLLISLQDVTREQLGYEKHGDVVKIFNNETKETTGFNIFNASSYLTID
CCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCEEEEEC
ENGPVALSETFVQDVNEILNRNGVEETLVVDLSPKFVVGYVESKEKHPNADKLSVCKVNV
CCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCEEEEEEEC
GEETLQIVCGAPNVDQGQKVVVAKVGAVMPSGLVIKDAELRGVPSSGMICSAKELDLPDA
CCCEEEEEECCCCCCCCCEEEEEEECCCCCCCEEEECCHHCCCCCCCCEEEHHCCCCCCC
PAEKGILVLEGDYEAGDAFQF
CCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.125 {2'-deoxyadenosine} 0.009 {ATP}} 0.2 {2-chloroadenosine} 0.11 {2-chloroadenosine} [C]

Substrates: ATP; @PHEN01.txt*phenylalanine!; tRNAPhe [C]

Specific reaction: ATP + @PHEN01.txt*phenylalanine! + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 3'-Deoxyadenosine 5'-triphosphate; 3'-Methyl adenosine 5'-triphosphate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377