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The map label for this gene is murC

Identifier: 16080031

GI number: 16080031

Start: 3048177

End: 3049475

Strand: Reverse

Name: murC

Synonym: BSU29790

Alternate gene names: 16080031

Gene position: 3049475-3048177 (Counterclockwise)

Preceding gene: 255767684

Following gene: 255767683

Centisome position: 72.34

GC content: 44.03

Gene sequence:

>1299_bases
ATGACTGTTTATCATTTTGTTGGAATAAAAGGGACCGGTATGAGTCCGCTTGCCCAAATACTTCATGATAATGGATATAC
TGTCCAAGGATCGGATATCGAAAAATTTATTTTTACGCAAACAGCGCTTGAAAAAAGAAATATTACGATTCTTCCTTTTA
GCGCGGAAAATATTAAACCCGGCATGACAGTCATCGCCGGAAACGCATTCCCAGACACGCATCCTGAAATAGAAAAAGCG
ATGTCTGAGGGAATTCCGGTGATACGTTATCATAAGTTTTTAGGCGACTACATGAAAAAATTCACGAGTGTTGCCGTTAC
GGGCGCACACGGCAAAACGTCGACTACGGGTCTGCTGGCTCACGTGATCCAAAACGCAAAACCGACGTCTTTCTTAATCG
GAGACGGAACAGGCCAGGGAAATGAAAACAGCGAATACTTTGTGTTTGAAGCGTGCGAATACCGCCGCCATTTCTTAAGC
TATCAGCCAGATTATGCGATTATGACGAATATTGATTTTGATCACCCTGATTACTTCTCAAGCATTGACGATGTATTTGA
CGCTTTCCAAGAGATGGCTCTTCAAGTCAACAAAGGCATTATTGCCTGCGGAGACGATGAGCATCTGCCGAAAATCCATG
CGAACGTCCCGGTTGTGTATTATGGCACGGGGGAAGAAAATGATTTTCAAGCCAGAAACATCGTAAAAAGCACGGAAGGG
ACAACTTTTGATGTCTTTGTCCGCAATACGTTCTATGATACGTTTTATATTCCTGCGTACGGCCACCACAATGTATTAAA
CTCATTGGCGGTCATTGCGTTATGCCATTATGAAGAAATTGATTCCAGCATCATTAAGCATGCTCTCAAATCCTTTGGGG
GCGTCAAACGCAGATTCAATGAGAAGCAGCTTGGGGATCAAGTGCTGATTGATGACTATGCCCATCATCCGACAGAAATA
AAAGTGACAATCGAGGCGGCAAGACAGAAGTATCCTGATCGGGAAATTGTCGCGGTATTCCAGCCTCATACATTTACCCG
GACGCAGCAGTTCCTTGACGAATTTGCAGAAAGCCTGAGCGGCGCCGACTGTGTGTATTTATGCGATATTTTCGGCTCAG
CCCGTGAGAATGCCGGAAAGCTGACGATCGGTGATCTCCAGGGAAAAATTCATAATGCCAAGCTGATTGAAGAAGATGAC
ACATCTGTTTTAAAAGCTCATGATAAAGCCGTTCTCATCTTCATGGGAGCAGGAGATATCCAAAAATATATGAGAGCCTA
CGAAAACGTCATGGCATAA

Upstream 100 bases:

>100_bases
AAAATACGCCGCTAAGGGAGGCTTTTCAGCCGCCGCGGTCGCAAATTGAAATGAGCGTCATATACTGGGGAACAGATAGA
CGTTTGTTGGAGGTACAATT

Downstream 100 bases:

>100_bases
TAAAAAGCAGTGATCTCACTGCTTTTTATTTATCTGATGGGAAAGCTTATTAAAGGATTTTCTTACATAACGGAGAATAG
TATGTATACAGCCCAGTACA

Product: UDP-N-acetylmuramate--L-alanine ligase

Products: NA

Alternate protein names: UDP-N-acetylmuramoyl-L-alanine synthetase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTVYHFVGIKGTGMSPLAQILHDNGYTVQGSDIEKFIFTQTALEKRNITILPFSAENIKPGMTVIAGNAFPDTHPEIEKA
MSEGIPVIRYHKFLGDYMKKFTSVAVTGAHGKTSTTGLLAHVIQNAKPTSFLIGDGTGQGNENSEYFVFEACEYRRHFLS
YQPDYAIMTNIDFDHPDYFSSIDDVFDAFQEMALQVNKGIIACGDDEHLPKIHANVPVVYYGTGEENDFQARNIVKSTEG
TTFDVFVRNTFYDTFYIPAYGHHNVLNSLAVIALCHYEEIDSSIIKHALKSFGGVKRRFNEKQLGDQVLIDDYAHHPTEI
KVTIEAARQKYPDREIVAVFQPHTFTRTQQFLDEFAESLSGADCVYLCDIFGSARENAGKLTIGDLQGKIHNAKLIEEDD
TSVLKAHDKAVLIFMGAGDIQKYMRAYENVMA

Sequences:

>Translated_432_residues
MTVYHFVGIKGTGMSPLAQILHDNGYTVQGSDIEKFIFTQTALEKRNITILPFSAENIKPGMTVIAGNAFPDTHPEIEKA
MSEGIPVIRYHKFLGDYMKKFTSVAVTGAHGKTSTTGLLAHVIQNAKPTSFLIGDGTGQGNENSEYFVFEACEYRRHFLS
YQPDYAIMTNIDFDHPDYFSSIDDVFDAFQEMALQVNKGIIACGDDEHLPKIHANVPVVYYGTGEENDFQARNIVKSTEG
TTFDVFVRNTFYDTFYIPAYGHHNVLNSLAVIALCHYEEIDSSIIKHALKSFGGVKRRFNEKQLGDQVLIDDYAHHPTEI
KVTIEAARQKYPDREIVAVFQPHTFTRTQQFLDEFAESLSGADCVYLCDIFGSARENAGKLTIGDLQGKIHNAKLIEEDD
TSVLKAHDKAVLIFMGAGDIQKYMRAYENVMA
>Mature_431_residues
TVYHFVGIKGTGMSPLAQILHDNGYTVQGSDIEKFIFTQTALEKRNITILPFSAENIKPGMTVIAGNAFPDTHPEIEKAM
SEGIPVIRYHKFLGDYMKKFTSVAVTGAHGKTSTTGLLAHVIQNAKPTSFLIGDGTGQGNENSEYFVFEACEYRRHFLSY
QPDYAIMTNIDFDHPDYFSSIDDVFDAFQEMALQVNKGIIACGDDEHLPKIHANVPVVYYGTGEENDFQARNIVKSTEGT
TFDVFVRNTFYDTFYIPAYGHHNVLNSLAVIALCHYEEIDSSIIKHALKSFGGVKRRFNEKQLGDQVLIDDYAHHPTEIK
VTIEAARQKYPDREIVAVFQPHTFTRTQQFLDEFAESLSGADCVYLCDIFGSARENAGKLTIGDLQGKIHNAKLIEEDDT
SVLKAHDKAVLIFMGAGDIQKYMRAYENVMA

Specific function: Cell wall formation

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786279, Length=463, Percent_Identity=30.2375809935205, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1790680, Length=386, Percent_Identity=30.0518134715026, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURC_BACSU (P40778)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- EMBL:   L31845
- PIR:   C69662
- RefSeq:   NP_390857.1
- ProteinModelPortal:   P40778
- SMR:   P40778
- EnsemblBacteria:   EBBACT00000002617
- GeneID:   937311
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29790
- NMPDR:   fig|224308.1.peg.2982
- GenoList:   BSU29790
- GeneTree:   EBGT00050000000630
- HOGENOM:   HBG476594
- OMA:   YHEFLGD
- ProtClustDB:   PRK00421
- BioCyc:   BSUB:BSU29790-MONOMER
- BRENDA:   6.3.2.8
- GO:   GO:0005737
- HAMAP:   MF_00046
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR016040
- InterPro:   IPR005758
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01082

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.8

Molecular weight: Translated: 48365; Mature: 48234

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVYHFVGIKGTGMSPLAQILHDNGYTVQGSDIEKFIFTQTALEKRNITILPFSAENIKP
CEEEEEEEECCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCEEEEEECCCCCCC
GMTVIAGNAFPDTHPEIEKAMSEGIPVIRYHKFLGDYMKKFTSVAVTGAHGKTSTTGLLA
CEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHH
HVIQNAKPTSFLIGDGTGQGNENSEYFVFEACEYRRHFLSYQPDYAIMTNIDFDHPDYFS
HHHHCCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
SIDDVFDAFQEMALQVNKGIIACGDDEHLPKIHANVPVVYYGTGEENDFQARNIVKSTEG
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHCCCC
TTFDVFVRNTFYDTFYIPAYGHHNVLNSLAVIALCHYEEIDSSIIKHALKSFGGVKRRFN
CEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EKQLGDQVLIDDYAHHPTEIKVTIEAARQKYPDREIVAVFQPHTFTRTQQFLDEFAESLS
HHHCCCEEEEHHCCCCCCEEEEEEEHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
GADCVYLCDIFGSARENAGKLTIGDLQGKIHNAKLIEEDDTSVLKAHDKAVLIFMGAGDI
CCCEEEEEEHHCCCCCCCCCEEEECCCCCCCCCEEECCCCCHHHEECCCEEEEEECCHHH
QKYMRAYENVMA
HHHHHHHHHHCC
>Mature Secondary Structure 
TVYHFVGIKGTGMSPLAQILHDNGYTVQGSDIEKFIFTQTALEKRNITILPFSAENIKP
EEEEEEEECCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCEEEEEECCCCCCC
GMTVIAGNAFPDTHPEIEKAMSEGIPVIRYHKFLGDYMKKFTSVAVTGAHGKTSTTGLLA
CEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHH
HVIQNAKPTSFLIGDGTGQGNENSEYFVFEACEYRRHFLSYQPDYAIMTNIDFDHPDYFS
HHHHCCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
SIDDVFDAFQEMALQVNKGIIACGDDEHLPKIHANVPVVYYGTGEENDFQARNIVKSTEG
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHCCCC
TTFDVFVRNTFYDTFYIPAYGHHNVLNSLAVIALCHYEEIDSSIIKHALKSFGGVKRRFN
CEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EKQLGDQVLIDDYAHHPTEIKVTIEAARQKYPDREIVAVFQPHTFTRTQQFLDEFAESLS
HHHCCCEEEEHHCCCCCCEEEEEEEHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
GADCVYLCDIFGSARENAGKLTIGDLQGKIHNAKLIEEDDTSVLKAHDKAVLIFMGAGDI
CCCEEEEEEHHCCCCCCCCCEEEECCCCCCCCCEEECCCCCHHHEECCCEEEEEECCHHH
QKYMRAYENVMA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8733232