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The map label for this gene is ytsJ

Identifier: 16079974

GI number: 16079974

Start: 2989900

End: 2991132

Strand: Reverse

Name: ytsJ

Synonym: BSU29220

Alternate gene names: 16079974

Gene position: 2991132-2989900 (Counterclockwise)

Preceding gene: 16079975

Following gene: 255767673

Centisome position: 70.95

GC content: 45.9

Gene sequence:

>1233_bases
ATGTCATTAAGAGAAGAAGCATTACACCTGCATAAAGTCAACCAGGGGAAACTGGAGTCTAAATCAAAAGTAGAGGTCAG
AAATGCGAAAGATTTAAGCCTCGCGTATTCTCCGGGTGTTGCAGAACCGTGTAAGGATATTCATGAAGACATTAACAAAG
TATATGATTATACAATGAAGGGGAACATGGTAGCAGTTGTAACTGACGGTACAGCTGTGCTTGGCCTTGGAAACATCGGC
CCTGAAGCGGCGCTTCCTGTTATGGAAGGGAAAGCAGTTCTTTTCAAAAGCTTTGCGGGCGTAGATGCGTTCCCGATTGC
TTTAAACACAAACGATGTTGATAAAATCGTTGAAACTGTAAAATTGCTGGAACCGACATTTGGCGGCGTCAACCTTGAAG
ATATTGCAGCGCCAAACTGCTTTATCATTGAAGAGCGCCTCAAAAAAGAAACAAACATCCCGGTTTTCCATGATGATCAG
CACGGTACTGCCATTGTAACCGTAGCAGGTCTTGTGAACGCGCTGAAACTGTCTGGAAAATCCATGTCATCCATTAAAGT
TGTCGCAAACGGCGCGGGTGCGGCTGGCATTGCGATTATCAAGCTTCTTCATCATTATGGCGTTCGCGACATTGTGATGT
GCGATTCCAAAGGGGCCATTTATGAAGGGCGCCCGAACGGCATGAACGATGTCAAAAATGAAGTGGCGAAGTTCACGAAC
CAAGATCGCAAAGACGGTTCTCTGAAAGATGTTATCGTTGATGCTGACGTATTTATCGGTGTCTCTGTAGCAGGCGCGTT
AACAAAAGAAATGGTTCAAAGCATGGCAAAAGATCCGATTATCTTTGCAATGGCAAATCCAAATCCGGAAATCATGCCGG
AAGATGCGCGTGAAGCCGGAGCAAGCGTTGTCGGAACTGGCCGTTCTGACTTCCCGAACCAAGTAAACAATGTTCTTGCA
TTCCCTGGTATTTTCCGCGGAGCGCTTGATGTGCGTGCCACTCATATTAACGAGGAAATGAAAATCGCAGCGGTTGAAGC
CATTGCTTCACTGGTTTCAGAAGATGAGCTTAGCGCAGACTATGTCATCCCTGCACCGTTTGATAAACGTGTTGCGCCTG
CTGTTGCGAAGGCTGTGGCAAAAGCTGCGATGGAAACAGGTGTTGCAAGAATCACTGTTGACCCTGAGGAAGTCGCAGAA
AAAACAAGAAAACTTACGATTATTGGTGAATAA

Upstream 100 bases:

>100_bases
TGATCCTGTTTAAATATTCAGATAGATTTACTCCGCAGAATGCAGCAATCAACGCAGCAGTATACGAATCAAAAAATATC
CATAAGGAGTGTTTAGGAGA

Downstream 100 bases:

>100_bases
TTTTAATTCATTCCAAAAATGGCAGCGCGTTGGCGGAGACCGGCGCGCTGTTCTCAAGTTAAACTGACGGCTGAAATTTT
TTTAGGGGGTTAGCCTTAAA

Product: NADP-dependent malic enzyme

Products: NA

Alternate protein names: NAD-ME 4

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSLREEALHLHKVNQGKLESKSKVEVRNAKDLSLAYSPGVAEPCKDIHEDINKVYDYTMKGNMVAVVTDGTAVLGLGNIG
PEAALPVMEGKAVLFKSFAGVDAFPIALNTNDVDKIVETVKLLEPTFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQ
HGTAIVTVAGLVNALKLSGKSMSSIKVVANGAGAAGIAIIKLLHHYGVRDIVMCDSKGAIYEGRPNGMNDVKNEVAKFTN
QDRKDGSLKDVIVDADVFIGVSVAGALTKEMVQSMAKDPIIFAMANPNPEIMPEDAREAGASVVGTGRSDFPNQVNNVLA
FPGIFRGALDVRATHINEEMKIAAVEAIASLVSEDELSADYVIPAPFDKRVAPAVAKAVAKAAMETGVARITVDPEEVAE
KTRKLTIIGE

Sequences:

>Translated_410_residues
MSLREEALHLHKVNQGKLESKSKVEVRNAKDLSLAYSPGVAEPCKDIHEDINKVYDYTMKGNMVAVVTDGTAVLGLGNIG
PEAALPVMEGKAVLFKSFAGVDAFPIALNTNDVDKIVETVKLLEPTFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQ
HGTAIVTVAGLVNALKLSGKSMSSIKVVANGAGAAGIAIIKLLHHYGVRDIVMCDSKGAIYEGRPNGMNDVKNEVAKFTN
QDRKDGSLKDVIVDADVFIGVSVAGALTKEMVQSMAKDPIIFAMANPNPEIMPEDAREAGASVVGTGRSDFPNQVNNVLA
FPGIFRGALDVRATHINEEMKIAAVEAIASLVSEDELSADYVIPAPFDKRVAPAVAKAVAKAAMETGVARITVDPEEVAE
KTRKLTIIGE
>Mature_409_residues
SLREEALHLHKVNQGKLESKSKVEVRNAKDLSLAYSPGVAEPCKDIHEDINKVYDYTMKGNMVAVVTDGTAVLGLGNIGP
EAALPVMEGKAVLFKSFAGVDAFPIALNTNDVDKIVETVKLLEPTFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQH
GTAIVTVAGLVNALKLSGKSMSSIKVVANGAGAAGIAIIKLLHHYGVRDIVMCDSKGAIYEGRPNGMNDVKNEVAKFTNQ
DRKDGSLKDVIVDADVFIGVSVAGALTKEMVQSMAKDPIIFAMANPNPEIMPEDAREAGASVVGTGRSDFPNQVNNVLAF
PGIFRGALDVRATHINEEMKIAAVEAIASLVSEDELSADYVIPAPFDKRVAPAVAKAVAKAAMETGVARITVDPEEVAEK
TRKLTIIGE

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family

Homologues:

Organism=Homo sapiens, GI62420882, Length=404, Percent_Identity=29.2079207920792, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI62420880, Length=404, Percent_Identity=29.2079207920792, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI239049447, Length=404, Percent_Identity=29.2079207920792, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI4505143, Length=395, Percent_Identity=25.0632911392405, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI4505145, Length=408, Percent_Identity=26.2254901960784, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI270265879, Length=320, Percent_Identity=28.125, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788806, Length=406, Percent_Identity=51.9704433497537, Blast_Score=403, Evalue=1e-114,
Organism=Escherichia coli, GI87081919, Length=441, Percent_Identity=28.3446712018141, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537199, Length=420, Percent_Identity=26.6666666666667, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322823, Length=310, Percent_Identity=30.6451612903226, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24646388, Length=451, Percent_Identity=27.7161862527716, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24646386, Length=451, Percent_Identity=27.7161862527716, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI78707242, Length=411, Percent_Identity=26.0340632603406, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI78707238, Length=411, Percent_Identity=26.0340632603406, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356279, Length=452, Percent_Identity=26.7699115044248, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI281362674, Length=452, Percent_Identity=26.7699115044248, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI281362672, Length=452, Percent_Identity=26.7699115044248, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI78707240, Length=394, Percent_Identity=25.6345177664975, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MAO4_BACSU (O34962)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   C70001
- RefSeq:   NP_390800.1
- ProteinModelPortal:   O34962
- SMR:   O34962
- EnsemblBacteria:   EBBACT00000002996
- GeneID:   937378
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29220
- NMPDR:   fig|224308.1.peg.2925
- GenoList:   BSU29220
- GeneTree:   EBGT00050000001535
- HOGENOM:   HBG285702
- OMA:   RATHINE
- PhylomeDB:   O34962
- ProtClustDB:   CLSK2460949
- BioCyc:   BSUB:BSU29220-MONOMER
- BRENDA:   1.1.1.38
- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000106
- PRINTS:   PR00072
- SMART:   SM00919

Pfam domain/function: PF00390 malic; PF03949 Malic_M

EC number: =1.1.1.38

Molecular weight: Translated: 43668; Mature: 43536

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: ACT_SITE 91-91 BINDING 159-159 BINDING 286-286

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLREEALHLHKVNQGKLESKSKVEVRNAKDLSLAYSPGVAEPCKDIHEDINKVYDYTMK
CCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHCEEEC
GNMVAVVTDGTAVLGLGNIGPEAALPVMEGKAVLFKSFAGVDAFPIALNTNDVDKIVETV
CCEEEEEECCEEEEEECCCCCHHCCCHHCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHH
KLLEPTFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQHGTAIVTVAGLVNALKLSGK
HHHCCCCCCCCHHHHCCCCEEEEHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHCCCC
SMSSIKVVANGAGAAGIAIIKLLHHYGVRDIVMCDSKGAIYEGRPNGMNDVKNEVAKFTN
CCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCHHHHHHHHHHHCC
QDRKDGSLKDVIVDADVFIGVSVAGALTKEMVQSMAKDPIIFAMANPNPEIMPEDAREAG
CCCCCCCHHEEEEECHHEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHCC
ASVVGTGRSDFPNQVNNVLAFPGIFRGALDVRATHINEEMKIAAVEAIASLVSEDELSAD
CEEEECCCCCCCHHHHHEEECCHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHCCCCCC
YVIPAPFDKRVAPAVAKAVAKAAMETGVARITVDPEEVAEKTRKLTIIGE
EEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCEEEEEC
>Mature Secondary Structure 
SLREEALHLHKVNQGKLESKSKVEVRNAKDLSLAYSPGVAEPCKDIHEDINKVYDYTMK
CHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHCEEEC
GNMVAVVTDGTAVLGLGNIGPEAALPVMEGKAVLFKSFAGVDAFPIALNTNDVDKIVETV
CCEEEEEECCEEEEEECCCCCHHCCCHHCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHH
KLLEPTFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQHGTAIVTVAGLVNALKLSGK
HHHCCCCCCCCHHHHCCCCEEEEHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHCCCC
SMSSIKVVANGAGAAGIAIIKLLHHYGVRDIVMCDSKGAIYEGRPNGMNDVKNEVAKFTN
CCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCHHHHHHHHHHHCC
QDRKDGSLKDVIVDADVFIGVSVAGALTKEMVQSMAKDPIIFAMANPNPEIMPEDAREAG
CCCCCCCHHEEEEECHHEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHCC
ASVVGTGRSDFPNQVNNVLAFPGIFRGALDVRATHINEEMKIAAVEAIASLVSEDELSAD
CEEEECCCCCCCHHHHHEEECCHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHCCCCCC
YVIPAPFDKRVAPAVAKAVAKAAMETGVARITVDPEEVAEKTRKLTIIGE
EEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377