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The map label for this gene is dnaB

Identifier: 16079951

GI number: 16079951

Start: 2964148

End: 2965566

Strand: Reverse

Name: dnaB

Synonym: BSU28990

Alternate gene names: 16079951

Gene position: 2965566-2964148 (Counterclockwise)

Preceding gene: 16079952

Following gene: 16079950

Centisome position: 70.35

GC content: 45.24

Gene sequence:

>1419_bases
ATGGCTGACTATTGGAAAGATGTACTGCCTGTAGACCCGTATGTGGTTAAAAGCAGATCGATGCTGCAGGATATTGACAG
ACAAATTATTACACAACTGTACCAGCCCTTAATCGGCCCTGTTGCATTCTCTCTTTATATGACATTGTGGGGAGAGCTTG
AACAAAACCGTCTGTGGGGCGGAGAGTCCACACATAGACAGCTGATGGGGATGACACAGTCCAACTTAAAAACGATCCAT
CAGGAACAGGGGAAGCTCGAGGGCATCGGATTGCTGAAAGTATATATGAAAGAATCCGAGCGGCAGGAGCGCCTCTTTAT
ATATGAACTCCTTCCGCCGCTCAGACCAAATGAATTTTTTGAAGACGGAATGCTGAATGTTTTTCTGTATAATAGGGTCG
GAAAAACGAAATATCAGCAATTAAAACAATTTTTTACGCACCCGGCCATTTCAGAAGATGCGAAGGACATTACGCGTCCG
TTTAATCATGCGTTTGAATCGCTGCAGCCGAGTGAATGGAAGCTCACATCCGATATGGAGGAAACGGTAAGGCTTGCCGA
GGGGTCCGAGTATACTTCTGTCGGACAGTCTCCGTCCTACACCATAACGGAGGATGTGTTTGATTTTGATTTGTTTTTAG
CGGGACTTTCTGAGACGATGATACCGAGAAAAGCGATGACCCAGCAGGTGCGGGACACAATCAAAAAGCTTTCTTATCTG
TACGGAATTGACCCTTTGCAAATGCAAAATGTTGTGATGAGCGCAATTGATGAGCGTGACGTCATTACGACGGAGGCATT
AAGAAAAGCGGCGAGCGACTGGTATCAAATTGAAAGAAACGGACAGCTGCCCGATTTGGTGGAAAAAACACAGCCGGTGC
ATCTGCGTGAAGGCGAACAACCGGCAGAAGAGGATTCTCTGGATGGGAAGCTGATTGCATTGCTGGAGGCGATTTCCCCG
AAGAAGCTTCTGCAGGACATTGCTGACGGAACAGAGCCGTCAAAAGCTGATCTGAAGATCATAGAAGAAATTATGTTCGA
ACAGAAGCTCGAGCCAGGCGTCACAAATGTGTTAATTTATTATGTCATGCTGAAAACCGACATGAAGCTGTCGAAAAACT
ATATCCAAAAAATCGCTTCGCATTGGGCACGCAAAAAGGTGAAAACGGTCAGGGAAGCGATGAAGCTCGCAATAGAAGAA
AACCGCCAGTATCTTGAATGGGCTGAGGGAAAAACAAAGTCTTCGAAACGAAACCAAAAAGTGATTCGTGAAGAAAAGCT
TCCTGATTGGATGACAGAAAAGGAAACAGCGTCCGATTCAGAATCCGGACAGCAGAAGCTGCATCCGCAGGATTTGGAAG
AACAGAAGAAAAAGATGATGGAAGAAATGCAAAAACTGAAAAAATACTCTGCCTATTAA

Upstream 100 bases:

>100_bases
TTCTTCTAGCTCTTTTCTCATGTGCAAAATTGGCATTTGATAGATATAAGAAACGGTGTAAAATGAAAGGTAGAAACAAA
TTCGGAAGGTTTGAGAATGT

Downstream 100 bases:

>100_bases
AGACAGAGATGCGGGGTGAAGGAATAGATGGAACCAATCGGCCGTTCCCTGCAGGGCGTAACCGGCAGGCCGGATTTCCA
AAAACGTCTTGAACAAATGA

Product: helicase loading protein; replication initiation membrane attachment protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MADYWKDVLPVDPYVVKSRSMLQDIDRQIITQLYQPLIGPVAFSLYMTLWGELEQNRLWGGESTHRQLMGMTQSNLKTIH
QEQGKLEGIGLLKVYMKESERQERLFIYELLPPLRPNEFFEDGMLNVFLYNRVGKTKYQQLKQFFTHPAISEDAKDITRP
FNHAFESLQPSEWKLTSDMEETVRLAEGSEYTSVGQSPSYTITEDVFDFDLFLAGLSETMIPRKAMTQQVRDTIKKLSYL
YGIDPLQMQNVVMSAIDERDVITTEALRKAASDWYQIERNGQLPDLVEKTQPVHLREGEQPAEEDSLDGKLIALLEAISP
KKLLQDIADGTEPSKADLKIIEEIMFEQKLEPGVTNVLIYYVMLKTDMKLSKNYIQKIASHWARKKVKTVREAMKLAIEE
NRQYLEWAEGKTKSSKRNQKVIREEKLPDWMTEKETASDSESGQQKLHPQDLEEQKKKMMEEMQKLKKYSAY

Sequences:

>Translated_472_residues
MADYWKDVLPVDPYVVKSRSMLQDIDRQIITQLYQPLIGPVAFSLYMTLWGELEQNRLWGGESTHRQLMGMTQSNLKTIH
QEQGKLEGIGLLKVYMKESERQERLFIYELLPPLRPNEFFEDGMLNVFLYNRVGKTKYQQLKQFFTHPAISEDAKDITRP
FNHAFESLQPSEWKLTSDMEETVRLAEGSEYTSVGQSPSYTITEDVFDFDLFLAGLSETMIPRKAMTQQVRDTIKKLSYL
YGIDPLQMQNVVMSAIDERDVITTEALRKAASDWYQIERNGQLPDLVEKTQPVHLREGEQPAEEDSLDGKLIALLEAISP
KKLLQDIADGTEPSKADLKIIEEIMFEQKLEPGVTNVLIYYVMLKTDMKLSKNYIQKIASHWARKKVKTVREAMKLAIEE
NRQYLEWAEGKTKSSKRNQKVIREEKLPDWMTEKETASDSESGQQKLHPQDLEEQKKKMMEEMQKLKKYSAY
>Mature_471_residues
ADYWKDVLPVDPYVVKSRSMLQDIDRQIITQLYQPLIGPVAFSLYMTLWGELEQNRLWGGESTHRQLMGMTQSNLKTIHQ
EQGKLEGIGLLKVYMKESERQERLFIYELLPPLRPNEFFEDGMLNVFLYNRVGKTKYQQLKQFFTHPAISEDAKDITRPF
NHAFESLQPSEWKLTSDMEETVRLAEGSEYTSVGQSPSYTITEDVFDFDLFLAGLSETMIPRKAMTQQVRDTIKKLSYLY
GIDPLQMQNVVMSAIDERDVITTEALRKAASDWYQIERNGQLPDLVEKTQPVHLREGEQPAEEDSLDGKLIALLEAISPK
KLLQDIADGTEPSKADLKIIEEIMFEQKLEPGVTNVLIYYVMLKTDMKLSKNYIQKIASHWARKKVKTVREAMKLAIEEN
RQYLEWAEGKTKSSKRNQKVIREEKLPDWMTEKETASDSESGQQKLHPQDLEEQKKKMMEEMQKLKKYSAY

Specific function: Probable component of primosome involved in the initiation of DNA replication. It is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110

COG id: COG3611

COG function: function code L; Replication initiation/membrane attachment protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAB_BACSU (P07908)

Other databases:

- EMBL:   M15183
- EMBL:   X04963
- EMBL:   Z75208
- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   B26580
- RefSeq:   NP_390777.1
- ProteinModelPortal:   P07908
- SMR:   P07908
- EnsemblBacteria:   EBBACT00000000935
- GeneID:   937396
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28990
- NMPDR:   fig|224308.1.peg.2902
- GenoList:   BSU28990
- GeneTree:   EBGT00050000001335
- HOGENOM:   HBG314027
- OMA:   PVMNVLI
- ProtClustDB:   CLSK2752474
- BioCyc:   BSUB:BSU28990-MONOMER
- InterPro:   IPR006343

Pfam domain/function: PF07261 DnaB_2

EC number: NA

Molecular weight: Translated: 54891; Mature: 54759

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCHHCCHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADYWKDVLPVDPYVVKSRSMLQDIDRQIITQLYQPLIGPVAFSLYMTLWGELEQNRLWG
CCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GESTHRQLMGMTQSNLKTIHQEQGKLEGIGLLKVYMKESERQERLFIYELLPPLRPNEFF
CCHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
EDGMLNVFLYNRVGKTKYQQLKQFFTHPAISEDAKDITRPFNHAFESLQPSEWKLTSDME
HCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
ETVRLAEGSEYTSVGQSPSYTITEDVFDFDLFLAGLSETMIPRKAMTQQVRDTIKKLSYL
HHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YGIDPLQMQNVVMSAIDERDVITTEALRKAASDWYQIERNGQLPDLVEKTQPVHLREGEQ
HCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCC
PAEEDSLDGKLIALLEAISPKKLLQDIADGTEPSKADLKIIEEIMFEQKLEPGVTNVLIY
CCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
YVMLKTDMKLSKNYIQKIASHWARKKVKTVREAMKLAIEENRQYLEWAEGKTKSSKRNQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHH
VIREEKLPDWMTEKETASDSESGQQKLHPQDLEEQKKKMMEEMQKLKKYSAY
HHHHHCCCCHHCCHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3027697; 3027671; 8969504; 9387221; 9384377