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The map label for this gene is glnH

Identifier: 16079797

GI number: 16079797

Start: 2803108

End: 2803929

Strand: Direct

Name: glnH

Synonym: BSU27440

Alternate gene names: 16079797

Gene position: 2803108-2803929 (Clockwise)

Preceding gene: 16079796

Following gene: 16079798

Centisome position: 66.49

GC content: 41.0

Gene sequence:

>822_bases
ATGAAAAAGATTTTTTCTTTAGCATTGATTTCTTTATTCGCTGTCATCTTGCTTGCCGCTTGCGGCAGCAAGGGATCAAA
CGGAGAAGCCTCAAAGGAGAGCAAAAAAGATACGCTAGCCGCCATTAAAGACAATGACAAAATTGTCTTCGGCGTAAAAA
CGGACACTCGACTGTTCGGTCTTAAAAATCCAAGCTCAGGTGAGATTGAGGGTTTTGATATTGATATTGCTAAACAAATC
GCTAAAGACATTTTAGGTGATGAGAAAAAAGCTCAATTTAAAGAGGTCACCTCAAAAACAAGGATTCCTATGCTGCAAAA
CGGGGATATCGACGCCATTGTCGCCACAATGACGATCACTGAAGAACGAAAAAAGGAAGTTGACTTTTCTGATGTCTATT
TCGAGGCCGGGCAATCGCTTCTCGTGAAAAAAGGCAGCAAAATCAAGAGTGTCGAGAATCTTGGCAAAGGCTCAAAAGTT
CTTGCAGTAAAAGGCTCCACCTCCTCTCAAAACATTCGTGAAAAGGCGCCTGAAGCATCTGTTTTAGAATTTGAAAACTA
CGCAGAAGCCTTTACAGCCCTTAAATCGGGGCAAGGCGATGCCCTCACCACAGATAATGCAATTTTATATGGAATGGCTG
ATGAAAATAAAAATTATCAATTAACAGGAAAGCCTTTTACAGATGAGCCTTACGGGATCGCCGTGAAAAAAGGGCAATCT
GCTCTCGCGAAAGAAATCAACGCTTCACTTAAAAAGATGAAGTCGGATGGCAGATACGATGAAATTTATAAAAAATGGAT
CAAAGAAGACCCAGCTGAATAA

Upstream 100 bases:

>100_bases
AGCAGTGCCGGCTGAATTTTACGCGAACCCGAAGGAAGAACGGGCCCGCTTATTCTTAAGCCGTATTTTAAATCATTAAA
ACAGAAATGGGGGAGCTTGT

Downstream 100 bases:

>100_bases
CAACCATCCCGGGATGAGCAGGCGATGCTCATCCCAATTTGATAAGGAGTGAATAGGCAGATGAATGTGTCAATTCTTTT
CGATAACTTCAGCATGTATA

Product: glutamine ABC transporter glutamine-binding lipoprotein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKKIFSLALISLFAVILLAACGSKGSNGEASKESKKDTLAAIKDNDKIVFGVKTDTRLFGLKNPSSGEIEGFDIDIAKQI
AKDILGDEKKAQFKEVTSKTRIPMLQNGDIDAIVATMTITEERKKEVDFSDVYFEAGQSLLVKKGSKIKSVENLGKGSKV
LAVKGSTSSQNIREKAPEASVLEFENYAEAFTALKSGQGDALTTDNAILYGMADENKNYQLTGKPFTDEPYGIAVKKGQS
ALAKEINASLKKMKSDGRYDEIYKKWIKEDPAE

Sequences:

>Translated_273_residues
MKKIFSLALISLFAVILLAACGSKGSNGEASKESKKDTLAAIKDNDKIVFGVKTDTRLFGLKNPSSGEIEGFDIDIAKQI
AKDILGDEKKAQFKEVTSKTRIPMLQNGDIDAIVATMTITEERKKEVDFSDVYFEAGQSLLVKKGSKIKSVENLGKGSKV
LAVKGSTSSQNIREKAPEASVLEFENYAEAFTALKSGQGDALTTDNAILYGMADENKNYQLTGKPFTDEPYGIAVKKGQS
ALAKEINASLKKMKSDGRYDEIYKKWIKEDPAE
>Mature_273_residues
MKKIFSLALISLFAVILLAACGSKGSNGEASKESKKDTLAAIKDNDKIVFGVKTDTRLFGLKNPSSGEIEGFDIDIAKQI
AKDILGDEKKAQFKEVTSKTRIPMLQNGDIDAIVATMTITEERKKEVDFSDVYFEAGQSLLVKKGSKIKSVENLGKGSKV
LAVKGSTSSQNIREKAPEASVLEFENYAEAFTALKSGQGDALTTDNAILYGMADENKNYQLTGKPFTDEPYGIAVKKGQS
ALAKEINASLKKMKSDGRYDEIYKKWIKEDPAE

Specific function: Part of the ABC transporter complex glnHMPQ involved in glutamine transport

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family

Homologues:

Organism=Escherichia coli, GI1787031, Length=228, Percent_Identity=34.6491228070175, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1787088, Length=210, Percent_Identity=32.3809523809524, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787085, Length=232, Percent_Identity=31.0344827586207, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1786876, Length=242, Percent_Identity=28.5123966942149, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788228, Length=266, Percent_Identity=28.9473684210526, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788648, Length=217, Percent_Identity=31.3364055299539, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788649, Length=211, Percent_Identity=27.9620853080569, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GLNH_BACSU (O34563)

Other databases:

- EMBL:   Z69371
- EMBL:   AL009126
- PIR:   D69633
- RefSeq:   NP_390621.1
- HSSP:   P19491
- ProteinModelPortal:   O34563
- SMR:   O34563
- EnsemblBacteria:   EBBACT00000003264
- GeneID:   938139
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27440
- NMPDR:   fig|224308.1.peg.2746
- GenoList:   BSU27440
- GeneTree:   EBGT00050000000372
- HOGENOM:   HBG698334
- OMA:   ESKTITW
- PhylomeDB:   O34563
- ProtClustDB:   CLSK887667
- BioCyc:   BSUB:BSU27440-MONOMER
- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313
- PANTHER:   PTHR18966
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3

EC number: NA

Molecular weight: Translated: 29756; Mature: 29756

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS01039 SBP_BACTERIAL_3; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIFSLALISLFAVILLAACGSKGSNGEASKESKKDTLAAIKDNDKIVFGVKTDTRLFG
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEECCCEEEEEEECCCEEEE
LKNPSSGEIEGFDIDIAKQIAKDILGDEKKAQFKEVTSKTRIPMLQNGDIDAIVATMTIT
CCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEC
EERKKEVDFSDVYFEAGQSLLVKKGSKIKSVENLGKGSKVLAVKGSTSSQNIREKAPEAS
HHHHHCCCHHHHHHHCCCCEEHHCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCH
VLEFENYAEAFTALKSGQGDALTTDNAILYGMADENKNYQLTGKPFTDEPYGIAVKKGQS
HHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCEEEECCCCCCCCCEEEEECCHH
ALAKEINASLKKMKSDGRYDEIYKKWIKEDPAE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKIFSLALISLFAVILLAACGSKGSNGEASKESKKDTLAAIKDNDKIVFGVKTDTRLFG
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEECCCEEEEEEECCCEEEE
LKNPSSGEIEGFDIDIAKQIAKDILGDEKKAQFKEVTSKTRIPMLQNGDIDAIVATMTIT
CCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEC
EERKKEVDFSDVYFEAGQSLLVKKGSKIKSVENLGKGSKVLAVKGSTSSQNIREKAPEAS
HHHHHCCCHHHHHHHCCCCEEHHCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCH
VLEFENYAEAFTALKSGQGDALTTDNAILYGMADENKNYQLTGKPFTDEPYGIAVKKGQS
HHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCEEEECCCCCCCCCEEEEECCHH
ALAKEINASLKKMKSDGRYDEIYKKWIKEDPAE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 9384377