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The map label for this gene is pbpI

Identifier: 16079785

GI number: 16079785

Start: 2789686

End: 2791440

Strand: Reverse

Name: pbpI

Synonym: BSU27310

Alternate gene names: 16079785

Gene position: 2791440-2789686 (Counterclockwise)

Preceding gene: 16079786

Following gene: 16079784

Centisome position: 66.22

GC content: 43.82

Gene sequence:

>1755_bases
ATGAAGATATCGAAACGAATGAAGCTGGCAGTCATCGCTTTTTTGATTGTATTTTTTCTGCTTCTGCTGAGACTTGCAGA
AATCCAGCTGTTTTTCACAGAATCATTTTCTAAAAAGAAAATTAATTTGATTCAAGAAAGTGTGAAACAGCGGACGGAGG
AAGTGCTCATTTCAGATGGAAGAGGCTCTTTTTTAGATCGAAACGGGCGGGCGCTGACAGGACAAAGCGAGCCTGCGGTT
GTTTTGTTCCCTTTTCTGCTGACGCAGGATTGGCCGATCAAAAAAGTTGCGGACATCCTCGGCATGTCTGAAGATGATTT
GCGCCAGACACTAGGTCAGGCAAAAAAACCGGTCATTTTGCAGCAAAAGAAAATCAAAACACTTTCAAAACAATCTATCA
CAAAAATCAATTCTTTGAAATATCCCGGCATTTACGGCGTATATATGGAGAATGAAGACAAGCCCAGCCTTGCTTCACAT
ACGATAGGGAGCACGAACCAAGATCCGGCGCTGCTTCGCAAGAAATATCCTGACAAGGAGAGCCTTCCGATTACAACAGA
AATCGGAACAACCGGTTTAGAACGGACATTTGACGAGTTTTTGCTTCCGGAACAAGACACAAAGCTTTTATATCATGTGG
ACGGAAAAGGAAACCCTTTGTTTGGCATGGACGTCAAATATACAGCTGAAGCCAATACATTTTATCCGCTCCAGATCAAA
ACAACGATCGATCAAAGCATTCAAAAAGCGATGGAGGAGGTATTGGATGAACAGGGGCTGAAAAAGGGAGGAGCTGTGCT
GCTGGACATCGAAAATAGCAGTGTTCTGGGAATTGTAAGCAAACCAGATGCAGACGTGTCCCGGCAAAACACACTTCAAA
ATTATATGCTGACGCCAATCTATCCCGGATCAGTTTTTAAAACAGTCATTGCCGCAGCTGCAATCGAAAATAATATGGTG
AAGCCTAGTCAAACCTTTAATTGCAATTTAAACCTGTACGGGGAACCGGGGGACGACAAAGGCACCTTGTCTTTTGATGA
AAGTTTTGCCCAAAGCTGTAATTACACATTTACGAGTCTTGCAGAACAATTAATGAAAAAAGACAGCTCGGTTATTGAAG
ATATGTCCGAGAAGCTGGCTCTCACTGATCGGGCAGGCTGGGAGGGAAAACTGTACCACGAAACCGATTTCCGCCAGCTG
TATAACGAAAAAAGCGGCGTGATTTGGGGGGATGAAAAAGACAAATCAGTCAAAAAAGCCATTGCGCAAACAGCAATCGG
GCAGAAAAATGTAAAGGTAACGCCGCTGGAGGTAGCCAATATGATGGCGACAATCGCCCGCGGCGGAGAAAAACGGCAGG
TGAAAATTGCCGAACAAATTGAGTATAAGAACGGCACAACGCTCGTTACGTTTAAAGATCAGAAGCTCAAAGGGGAAACG
ATAGACAAATATACGTCACAGCAGCTTCAAAAAATACTGCGGCGGGTAGTAGAATCTCCTTCCGGAACGGGCAGAAGGTT
TCAAGATCTTCCGTATACTGTAGCAGGGAAGTCGGGCACAGCCCAGACAGGCAAGCTTTCAAAAGAGAAGGAAACGCTCT
ACGAAAAATGGTTTGCCGGATATTTTCCCGCGGATAAGCCGAAATACGCTCTAGTTGTTTTACACATGGATACACCGGGA
GATAAGGCTCTGACAAATTCGGTGTTTTATGATATTGTTAAAAAAGTACACGAAATTGAAATCAATCAGAAATAG

Upstream 100 bases:

>100_bases
AAACACCGGGCGGAGAAATGCTCGTGAAAATTGTGAAAATTTCATAATATCTGTTTAGCAGCGAAACACCTCGTCCACAA
TGTGGGTGAGGTGTTTTTTT

Downstream 100 bases:

>100_bases
AACAACTTCTCAGCACATGTTAAAATATATGCTGAGCCAAGAGGGATAAAGGAGTGCAGAATTATTGAGCAATAATCAAT
CTCGTTATGAAAATCGTGAT

Product: penicillin-binding protein PBP4B

Products: NA

Alternate protein names: PBP-4B; PBP4b; Penicillin-binding protein I

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MKISKRMKLAVIAFLIVFFLLLLRLAEIQLFFTESFSKKKINLIQESVKQRTEEVLISDGRGSFLDRNGRALTGQSEPAV
VLFPFLLTQDWPIKKVADILGMSEDDLRQTLGQAKKPVILQQKKIKTLSKQSITKINSLKYPGIYGVYMENEDKPSLASH
TIGSTNQDPALLRKKYPDKESLPITTEIGTTGLERTFDEFLLPEQDTKLLYHVDGKGNPLFGMDVKYTAEANTFYPLQIK
TTIDQSIQKAMEEVLDEQGLKKGGAVLLDIENSSVLGIVSKPDADVSRQNTLQNYMLTPIYPGSVFKTVIAAAAIENNMV
KPSQTFNCNLNLYGEPGDDKGTLSFDESFAQSCNYTFTSLAEQLMKKDSSVIEDMSEKLALTDRAGWEGKLYHETDFRQL
YNEKSGVIWGDEKDKSVKKAIAQTAIGQKNVKVTPLEVANMMATIARGGEKRQVKIAEQIEYKNGTTLVTFKDQKLKGET
IDKYTSQQLQKILRRVVESPSGTGRRFQDLPYTVAGKSGTAQTGKLSKEKETLYEKWFAGYFPADKPKYALVVLHMDTPG
DKALTNSVFYDIVKKVHEIEINQK

Sequences:

>Translated_584_residues
MKISKRMKLAVIAFLIVFFLLLLRLAEIQLFFTESFSKKKINLIQESVKQRTEEVLISDGRGSFLDRNGRALTGQSEPAV
VLFPFLLTQDWPIKKVADILGMSEDDLRQTLGQAKKPVILQQKKIKTLSKQSITKINSLKYPGIYGVYMENEDKPSLASH
TIGSTNQDPALLRKKYPDKESLPITTEIGTTGLERTFDEFLLPEQDTKLLYHVDGKGNPLFGMDVKYTAEANTFYPLQIK
TTIDQSIQKAMEEVLDEQGLKKGGAVLLDIENSSVLGIVSKPDADVSRQNTLQNYMLTPIYPGSVFKTVIAAAAIENNMV
KPSQTFNCNLNLYGEPGDDKGTLSFDESFAQSCNYTFTSLAEQLMKKDSSVIEDMSEKLALTDRAGWEGKLYHETDFRQL
YNEKSGVIWGDEKDKSVKKAIAQTAIGQKNVKVTPLEVANMMATIARGGEKRQVKIAEQIEYKNGTTLVTFKDQKLKGET
IDKYTSQQLQKILRRVVESPSGTGRRFQDLPYTVAGKSGTAQTGKLSKEKETLYEKWFAGYFPADKPKYALVVLHMDTPG
DKALTNSVFYDIVKKVHEIEINQK
>Mature_584_residues
MKISKRMKLAVIAFLIVFFLLLLRLAEIQLFFTESFSKKKINLIQESVKQRTEEVLISDGRGSFLDRNGRALTGQSEPAV
VLFPFLLTQDWPIKKVADILGMSEDDLRQTLGQAKKPVILQQKKIKTLSKQSITKINSLKYPGIYGVYMENEDKPSLASH
TIGSTNQDPALLRKKYPDKESLPITTEIGTTGLERTFDEFLLPEQDTKLLYHVDGKGNPLFGMDVKYTAEANTFYPLQIK
TTIDQSIQKAMEEVLDEQGLKKGGAVLLDIENSSVLGIVSKPDADVSRQNTLQNYMLTPIYPGSVFKTVIAAAAIENNMV
KPSQTFNCNLNLYGEPGDDKGTLSFDESFAQSCNYTFTSLAEQLMKKDSSVIEDMSEKLALTDRAGWEGKLYHETDFRQL
YNEKSGVIWGDEKDKSVKKAIAQTAIGQKNVKVTPLEVANMMATIARGGEKRQVKIAEQIEYKNGTTLVTFKDQKLKGET
IDKYTSQQLQKILRRVVESPSGTGRRFQDLPYTVAGKSGTAQTGKLSKEKETLYEKWFAGYFPADKPKYALVVLHMDTPG
DKALTNSVFYDIVKKVHEIEINQK

Specific function: Penicillin-binding protein with an unknown catalytic activity. Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active site of these p

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBPI_BACSU (O32032)

Other databases:

- EMBL:   AL009126
- PIR:   A69980
- RefSeq:   NP_390609.1
- ProteinModelPortal:   O32032
- EnsemblBacteria:   EBBACT00000000763
- GeneID:   936016
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27310
- NMPDR:   fig|224308.1.peg.2734
- GenoList:   BSU27310
- GeneTree:   EBGT00070000032156
- HOGENOM:   HBG314114
- OMA:   KISKQTP
- ProtClustDB:   CLSK887662
- BioCyc:   BSUB:BSU27310-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase; SSF56519 PBP_dimer; SSF56601 PBP_transp_fold

EC number: NA

Molecular weight: Translated: 65523; Mature: 65523

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: ACT_SITE 304-304

Signals:

None

Transmembrane regions:

HASH(0xfb637e8)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISKRMKLAVIAFLIVFFLLLLRLAEIQLFFTESFSKKKINLIQESVKQRTEEVLISDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHEEECC
RGSFLDRNGRALTGQSEPAVVLFPFLLTQDWPIKKVADILGMSEDDLRQTLGQAKKPVIL
CCCCCCCCCCEECCCCCCCEEEEEHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEE
QQKKIKTLSKQSITKINSLKYPGIYGVYMENEDKPSLASHTIGSTNQDPALLRKKYPDKE
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHCCCCCC
SLPITTEIGTTGLERTFDEFLLPEQDTKLLYHVDGKGNPLFGMDVKYTAEANTFYPLQIK
CCCEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCEECCCEEEEECCCEEEEEEEE
TTIDQSIQKAMEEVLDEQGLKKGGAVLLDIENSSVLGIVSKPDADVSRQNTLQNYMLTPI
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHCEECCC
YPGSVFKTVIAAAAIENNMVKPSQTFNCNLNLYGEPGDDKGTLSFDESFAQSCNYTFTSL
CCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCEEHHHHHHHHCCCHHHHH
AEQLMKKDSSVIEDMSEKLALTDRAGWEGKLYHETDFRQLYNEKSGVIWGDEKDKSVKKA
HHHHHHCCHHHHHHHHHHHEECCCCCCCCEEECCHHHHHHHCCCCCEEECCCCHHHHHHH
IAQTAIGQKNVKVTPLEVANMMATIARGGEKRQVKIAEQIEYKNGTTLVTFKDQKLKGET
HHHHHCCCCCCEECHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCEEEEEECCCCCCCH
IDKYTSQQLQKILRRVVESPSGTGRRFQDLPYTVAGKSGTAQTGKLSKEKETLYEKWFAG
HHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHC
YFPADKPKYALVVLHMDTPGDKALTNSVFYDIVKKVHEIEINQK
CCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCH
>Mature Secondary Structure
MKISKRMKLAVIAFLIVFFLLLLRLAEIQLFFTESFSKKKINLIQESVKQRTEEVLISDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHEEECC
RGSFLDRNGRALTGQSEPAVVLFPFLLTQDWPIKKVADILGMSEDDLRQTLGQAKKPVIL
CCCCCCCCCCEECCCCCCCEEEEEHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEE
QQKKIKTLSKQSITKINSLKYPGIYGVYMENEDKPSLASHTIGSTNQDPALLRKKYPDKE
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHCCCCCC
SLPITTEIGTTGLERTFDEFLLPEQDTKLLYHVDGKGNPLFGMDVKYTAEANTFYPLQIK
CCCEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCEECCCEEEEECCCEEEEEEEE
TTIDQSIQKAMEEVLDEQGLKKGGAVLLDIENSSVLGIVSKPDADVSRQNTLQNYMLTPI
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHCEECCC
YPGSVFKTVIAAAAIENNMVKPSQTFNCNLNLYGEPGDDKGTLSFDESFAQSCNYTFTSL
CCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCEEHHHHHHHHCCCHHHHH
AEQLMKKDSSVIEDMSEKLALTDRAGWEGKLYHETDFRQLYNEKSGVIWGDEKDKSVKKA
HHHHHHCCHHHHHHHHHHHEECCCCCCCCEEECCHHHHHHHCCCCCEEECCCCHHHHHHH
IAQTAIGQKNVKVTPLEVANMMATIARGGEKRQVKIAEQIEYKNGTTLVTFKDQKLKGET
HHHHHCCCCCCEECHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCEEEEEECCCCCCCH
IDKYTSQQLQKILRRVVESPSGTGRRFQDLPYTVAGKSGTAQTGKLSKEKETLYEKWFAG
HHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHC
YFPADKPKYALVVLHMDTPGDKALTNSVFYDIVKKVHEIEINQK
CCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377